Restriction map for
pFN21ASDA1001
(Circular) (Six-base) MAPSORT of: pFN21ASDA1001.seq Check: 1740 from: 1 to: 6304
pFN21ASDA1001 6304 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2279 2279
Size: 6304
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3195 3489 4114 278
Size: 53 83 186 936 1659 294 625 2468
Fragments arranged by size:
2468 1659 936 625 294 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3647 1937
Size: 1710 4594
Acc65I G'GTAC_C
Cuts at: 3625 3625
Size: 6304
AclI AA'CG_TT
Cuts at: 1563 4431 4804 1563
Size: 2868 373 3063
Fragments arranged by size:
3063 2868 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3528 4367 5751 6019 1632
Size: 1896 839 1384 268 1917
Fragments arranged by size:
1917 1896 1384 839 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6107
AflIII A'CryG_T
Cuts at: 1203 5203 6180 1203
Size: 4000 977 1327
Fragments arranged by size:
4000 1327 977
AgeI A'CCGG_T
Cuts at: 3323 5135 3323
Size: 1812 4492
AhdI GACnn_n'nnGTC
Cuts at: 5033 5033
Size: 6304
AleI CACnn'nnGTG
Cuts at: 1166 2351 2615 1166
Size: 1185 264 4855
Fragments arranged by size:
4855 1185 264
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6304
AlwNI CAG_nnn'CTG
Cuts at: 3416 4047 5619 3416
Size: 631 1572 4101
Fragments arranged by size:
4101 1572 631
ApaLI G'TGCA_C
Cuts at: 4361 5517 4361
Size: 1156 5148
ApoI r'AATT_y
Cuts at: 1493 3613 3839 3991 6100 1493
Size: 2120 226 152 2109 1697
Fragments arranged by size:
2120 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4858 160
Size: 872 3826 1606
Fragments arranged by size:
3826 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6304
AvaI C'yCGr_G
Cuts at: 1870 1943 2283 3461 3629 1870
Size: 73 340 1178 168 4545
Fragments arranged by size:
4545 1178 340 168 73
BamHI G'GATC_C
Cuts at: 3634 4001 3634
Size: 367 5937
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3042 3625 5080 618
Size: 325 217 627 1255 583 1455 1842
Fragments arranged by size:
1842 1455 1255 627 583 325 217
BanII G_rGCy'C
Cuts at: 1391 2841 2944 3280 3623 1391
Size: 1450 103 336 343 4072
Fragments arranged by size:
4072 1450 343 336 103
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3405 928
Size: 971 1506 3827
Fragments arranged by size:
3827 1506 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4495 4529 1903
Size: 34 2558 34 3678
Fragments arranged by size:
3678 2558 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4196 5406 4196
Size: 1210 5094
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6304
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2310 4915 136
Size: 107 122 71 631 605 638 2605 1525
Fragments arranged by size:
2605 1525 638 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6299 6299
Size: 6304
BlpI GC'TnA_GC
Cuts at: 3721 3721
Size: 6304
Bme1580I G_kGCm'C
Cuts at: 1790 2781 3045 3231 3423 4365 5521 1790
Size: 991 264 186 192 942 1156 2573
Fragments arranged by size:
2573 1156 991 942 264 192 186
BmgBI CAC'GTC
Cuts at: 1318 2617 1318
Size: 1299 5005
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2194 2427 2701
Size: 416 53 83 399 24 777 233 274
Cuts at: 2701 4035 4993 442
Size: 1334 958 1753
Fragments arranged by size:
1753 1334 958 777 416 399 274 233
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2960 1056
Size: 1904 4400
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2517 3285 3317 3502 4964 1239
Size: 1278 768 32 185 1462 2579
Fragments arranged by size:
2579 1462 1278 768 185 32
Bpu10I CC'TnA_GC
Cuts at: 3360 3360
Size: 6304
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2689 3150 4429 5294 5592 2689
Size: 461 1279 865 298 3401
Fragments arranged by size:
3401 1279 865 461 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2369 4967 882
Size: 617 870 2598 2219
Fragments arranged by size:
2598 2219 870 617
BsaAI yAC'GTr
Cuts at: 493 1204 2776 3601 4095 493
Size: 711 1572 825 494 2702
Fragments arranged by size:
2702 1572 825 711 494
BsaBI GATnn'nnATC
Cuts at: 6298 6298
Size: 6304
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3192 3486 4111 4493
Size: 53 83 186 936 1659 294 625 382
Cuts at: 4493 5128 275
Size: 635 1451
Fragments arranged by size:
1659 1451 936 635 625 382 294 186
83 53
BsaWI w'CCGG_w
Cuts at: 3323 4737 5135 5409 5556 5967 3323
Size: 1414 398 274 147 411 3660
Fragments arranged by size:
3660 1414 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2515 2545 3500 3530 1178
Size: 30 165 30 1112 30 955 30 3952
Fragments arranged by size:
3952 1112 955 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6304
BseYI C'CCAG_C
Cuts at: 3052 5507 6003 3052
Size: 2455 496 3353
Fragments arranged by size:
3353 2455 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2853 1163
Size: 573 1117 4614
Fragments arranged by size:
4614 1117 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2305 3754 4515 5543 1540
Size: 275 186 304 1449 761 1028 2301
Fragments arranged by size:
2301 1449 1028 761 304 275 186
BsiHKAI G_wGCw'C
Cuts at: 3207 3353 4365 4450 5521 3207
Size: 146 1012 85 1071 3990
Fragments arranged by size:
3990 1071 1012 146 85
BsmI GAATG_Cn'
Cuts at: 3815 3908 3815
Size: 93 6211
BsmBI CGTCTCn'nnnn_
Cuts at: 3199 3199
Size: 6304
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2781 2841 2944 3045 3207 3231 3280
Size: 399 991 60 103 101 162 24 49
Cuts at: 3280 3353 3423 3623 4365 4450 5521 1391
Size: 73 70 200 742 85 1071 2174
Fragments arranged by size:
2174 1071 991 742 399 200 162 103
101 85 73 70 60 49 24
BspEI T'CCGG_A
Cuts at: 5967 5967
Size: 6304
BspHI T'CATG_A
Cuts at: 4193 5923 4193
Size: 1730 4574
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2279 3659 844
Size: 1435 1380 3489
Fragments arranged by size:
3489 1435 1380
BsrBI CCG'CTC
Cuts at: 1394 1823 2368 4191 1394
Size: 429 545 1823 3507
Fragments arranged by size:
3507 1823 545 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2326 3498 4799 4973 65
Size: 1183 1078 1172 1301 174 1396
Fragments arranged by size:
1396 1301 1183 1172 1078 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2300 2304 2659 3213 3323 4948 5135
Size: 110 490 4 355 554 110 1625 187
Cuts at: 5135 1700
Size: 2869
Fragments arranged by size:
2869 1625 554 490 355 187 110 110
4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6304
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6304
BssSI C'ACGA_G
Cuts at: 4364 5376 4364
Size: 1012 5292
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3235 794
Size: 2441 3863
BstBI TT'CG_AA
Cuts at: 2007 5108 2007
Size: 3101 3203
BstEII G'GTnAC_C
Cuts at: 1184 2168 1184
Size: 984 5320
BstYI r'GATC_y
Cuts at: 1972 3634 4001 4386 4403 5855 5941 5953 6299
Size: 1662 367 385 17 1452 86 12 346
Cuts at: 6299 1972
Size: 1977
Fragments arranged by size:
1977 1662 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2449 2473 2810 2936 513
Size: 552 957 427 24 337 126 3881
Fragments arranged by size:
3881 957 552 427 337 126 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5565
Fragments arranged by size:
5565 683 56
BtsI GCAGTG_nn'
Cuts at: 2202 2971 3099 3820 4614 4634 2202
Size: 769 128 721 794 20 3872
Fragments arranged by size:
3872 794 769 721 128 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6304
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2455 2490 2534 2569 529
Size: 35 1891 35 44 35 4264
Fragments arranged by size:
4264 1891 44 35 35 35
DraI TTT'AAA
Cuts at: 3608 3608
Size: 6304
DraIII CAC_nnn'GTG
Cuts at: 2573 2893 3235 2573
Size: 320 342 5642
Fragments arranged by size:
5642 342 320
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5311 809
Size: 507 3995 1802
Fragments arranged by size:
3995 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2148 2302 3211
Size: 54 1425 50 275 18 318 154 909
Cuts at: 3211 3751 4640 6269 8
Size: 540 889 1629 43
Fragments arranged by size:
1629 1425 909 889 540 318 275 154
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2302 3751 1537
Size: 275 490 1449 4090
Fragments arranged by size:
4090 1449 490 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4234 1353
Size: 2881 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3281 4891 5265 5411 387
Size: 1064 138 1692 1610 374 146 1280
Fragments arranged by size:
1692 1610 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2517 3285 3317 3502 3528 4367 4964
Size: 393 885 768 32 185 26 839 597
Cuts at: 4964 5751 6019 1239
Size: 787 268 1524
Fragments arranged by size:
1524 885 839 787 768 597 393 268
185 32 26
EcoNI CCTnn'n_nnAGG
Cuts at: 3149 3149
Size: 6304
EcoO109I rG'GnC_Cy
Cuts at: 2739 2972 3049 3356 3538 2739
Size: 233 77 307 182 5505
Fragments arranged by size:
5505 307 233 182 77
EcoRI G'AATT_C
Cuts at: 3613 3991 3613
Size: 378 5926
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2149 2149
Size: 6304
FspI TGC'GCA
Cuts at: 4810 4810
Size: 6304
HaeII r_GCGC'y
Cuts at: 1678 2810 3178 5451 1678
Size: 1132 368 2273 2531
Fragments arranged by size:
2531 2273 1132 368
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2567 2599 2839 2871 3500
Size: 32 135 32 995 32 240 32 629
Cuts at: 3500 3532 4945 4977 5019 5051 1373
Size: 32 1413 32 42 32 2626
Fragments arranged by size:
2626 1413 995 629 240 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2884 3648 3894 669
Size: 988 281 946 764 246 3079
Fragments arranged by size:
3079 988 946 764 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6304
HpaI GTT'AAC
Cuts at: 3894 3894
Size: 6304
KpnI G_GTAC'C
Cuts at: 3629 3629
Size: 6304
MfeI C'AATT_G
Cuts at: 3903 3903
Size: 6304
MluI A'CGCG_T
Cuts at: 6180 6180
Size: 6304
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3155 5418 5602 1305
Size: 1850 2263 184 2007
Fragments arranged by size:
2263 2007 1850 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4482
Fragments arranged by size:
4482 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2351 2615 2774 4262 4621 4780 518
Size: 648 1185 264 159 1488 359 159 2042
Fragments arranged by size:
2042 1488 1185 648 359 264 159 159
MspA1I CmG'CkG
Cuts at: 1606 2261 2733 3255 3263 3585 3720 4397 5545
Size: 655 472 522 8 322 135 677 1148
Cuts at: 5545 5790 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 655 522 472 322 245
135 8
NaeI GCC'GGC
Cuts at: 2306 2306
Size: 6304
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4795
Fragments arranged by size:
4795 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6304
NgoMIV G'CCGG_C
Cuts at: 2304 2304
Size: 6304
NheI G'CTAG_C
Cuts at: 1052 2956 1052
Size: 1904 4400
NotI GC'GGCC_GC
Cuts at: 3751 3751
Size: 6304
NspI r_CATG'y
Cuts at: 1132 1229 2773 3662 5207 1132
Size: 97 1544 889 1545 2229
Fragments arranged by size:
2229 1545 1544 889 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6304
PciI A'CATG_T
Cuts at: 5203 5203
Size: 6304
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2028 2515 1108
Size: 920 487 4897
Fragments arranged by size:
4897 920 487
PmeI GTTT'AAAC
Cuts at: 3608 3608
Size: 6304
PmlI CAC'GTG
Cuts at: 2776 2776
Size: 6304
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4373 4405 5886 5918 4373
Size: 32 1481 32 4759
Fragments arranged by size:
4759 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2972 3049 3538 2972
Size: 77 489 5738
Fragments arranged by size:
5738 489 77
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6304
PsiI TTA'TAA
Cuts at: 3874 3874
Size: 6304
PstI C_TGCA'G
Cuts at: 830 2400 3285 3369 3656 830
Size: 1570 885 84 287 3478
Fragments arranged by size:
3478 1570 885 287 84
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6304
PvuII CAG'CTG
Cuts at: 2261 3263 2261
Size: 1002 5302
SalI G'TCGA_C
Cuts at: 1936 3646 1936
Size: 1710 4594
SanDI GG'GwC_CC
Cuts at: 2972 3049 2972
Size: 77 6227
SbfI CC_TGCA'GG
Cuts at: 3656 3656
Size: 6304
ScaI AGT'ACT
Cuts at: 4552 6264 4552
Size: 1712 4592
SfcI C'TryA_G
Cuts at: 826 1045 2396 3281 3365 3652 4787 5468 5659
Size: 219 1351 885 84 287 1135 681 191
Cuts at: 5659 826
Size: 1471
Fragments arranged by size:
1471 1351 1135 885 681 287 219 191
84
SfiI GGCCn_nnn'nGGCC
Cuts at: 2310 2310
Size: 6304
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6304
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6304
SmaI CCC'GGG
Cuts at: 1872 2285 3631 1872
Size: 413 1346 4545
Fragments arranged by size:
4545 1346 413
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2668 3165 4408 5309 5571 820
Size: 197 926 725 497 1243 901 262 1553
Fragments arranged by size:
1553 1243 926 901 725 497 262 197
SnaBI TAC'GTA
Cuts at: 493 3601 4095 493
Size: 3108 494 2702
Fragments arranged by size:
3108 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6304
SphI G_CATG'C
Cuts at: 1132 3662 1132
Size: 2530 3774
SspI AAT'ATT
Cuts at: 5 52 4228 5
Size: 47 4176 2081
Fragments arranged by size:
4176 2081 47
StuI AGG'CCT
Cuts at: 1517 2361 3311 1517
Size: 844 950 4510
Fragments arranged by size:
4510 950 844
StyI C'CwwG_G
Cuts at: 513 1065 2022 2382 3743 513
Size: 552 957 360 1361 3074
Fragments arranged by size:
3074 1361 957 552 360
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4331 4348 4501 4686 5131 325
Size: 1255 2751 17 153 185 445 1498
Fragments arranged by size:
2751 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2522 4550 6262
Size: 274 80 33 51 1443 545 2028 1712
Cuts at: 6262 96
Size: 138
Fragments arranged by size:
2028 1712 1443 545 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6264 6296 6264
Size: 32 6272
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6304
XbaI T'CTAG_A
Cuts at: 3640 3640
Size: 6304
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6304
XmaI C'CCGG_G
Cuts at: 1870 2283 3629 1870
Size: 413 1346 4545
Fragments arranged by size:
4545 1346 413
XmnI GAAnn'nnTTC
Cuts at: 4433 4433
Size: 6304
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3193 3487 4112 276
Size: 53 83 186 936 1659 294 625 2468
Fragments arranged by size:
2468 1659 936 625 294 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI
SfoI SrfI SwaI XcmI