Restriction map for
pFN21ASDA0997
(Circular) (Six-base) MAPSORT of: pFN21ASDA0997.seq Check: 4120 from: 1 to: 6661
pFN21ASDA0997 6661 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4471 278
Size: 53 83 186 936 2935 2468
Fragments arranged by size:
2935 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4004 1937
Size: 2067 4594
Acc65I G'GTAC_C
Cuts at: 3982 3982
Size: 6661
AclI AA'CG_TT
Cuts at: 1563 4788 5161 1563
Size: 3225 373 3063
Fragments arranged by size:
3225 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2039 3138 3395 4724 6108 6376 1632
Size: 407 1099 257 1329 1384 268 1917
Fragments arranged by size:
1917 1384 1329 1099 407 268 257
AflII C'TTAA_G
Cuts at: 820 1017 3799 820
Size: 197 2782 3682
Fragments arranged by size:
3682 2782 197
AflIII A'CryG_T
Cuts at: 1203 2641 3851 5560 6537 1203
Size: 1438 1210 1709 977 1327
Fragments arranged by size:
1709 1438 1327 1210 977
AgeI A'CCGG_T
Cuts at: 5492 5492
Size: 6661
AhdI GACnn_n'nnGTC
Cuts at: 5390 5390
Size: 6661
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6661
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2217 1184
Size: 1033 5628
AlwNI CAG_nnn'CTG
Cuts at: 4404 5976 4404
Size: 1572 5089
ApaLI G'TGCA_C
Cuts at: 4718 5874 4718
Size: 1156 5505
ApoI r'AATT_y
Cuts at: 1493 2822 3970 4196 4348 6457 1493
Size: 1329 1148 226 152 2109 1697
Fragments arranged by size:
2109 1697 1329 1148 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5215 160
Size: 872 4183 1606
Fragments arranged by size:
4183 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6661
AvaI C'yCGr_G
Cuts at: 1870 1943 3986 1870
Size: 73 2043 4545
Fragments arranged by size:
4545 2043 73
BamHI G'GATC_C
Cuts at: 3991 4358 3991
Size: 367 6294
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3982 5437 618
Size: 325 217 627 2195 1455 1842
Fragments arranged by size:
2195 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3441 3980 1391
Size: 2050 539 4072
Fragments arranged by size:
4072 2050 539
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2370 928
Size: 971 471 5219
Fragments arranged by size:
5219 971 471
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4852 4886 1903
Size: 34 2915 34 3678
Fragments arranged by size:
3678 2915 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3593 4553 5763 3593
Size: 960 1210 4491
Fragments arranged by size:
4491 1210 960
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6661
BfrBI ATG'CAT
Cuts at: 3284 3284
Size: 6661
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5272 136
Size: 107 122 71 631 605 3600 1525
Fragments arranged by size:
3600 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2765 3445 6656 2765
Size: 680 3211 2770
Fragments arranged by size:
3211 2770 680
BlpI GC'TnA_GC
Cuts at: 4078 4078
Size: 6661
Bme1580I G_kGCm'C
Cuts at: 1790 4722 5878 1790
Size: 2932 1156 2573
Fragments arranged by size:
2932 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6661
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4392 5350 442
Size: 416 53 83 399 24 2975 958 1753
Fragments arranged by size:
2975 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6661
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2281 5321 1239
Size: 1042 3040 2579
Fragments arranged by size:
3040 2579 1042
Bpu10I CC'TnA_GC
Cuts at: 2580 2580
Size: 6661
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4786 5651 5949 4786
Size: 865 298 5498
Fragments arranged by size:
5498 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5324 882
Size: 617 3825 2219
Fragments arranged by size:
3825 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3958 4452 493
Size: 711 2754 494 2702
Fragments arranged by size:
2754 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 6655 6655
Size: 6661
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4468 4850 5485 275
Size: 53 83 186 936 2935 382 635 1451
Fragments arranged by size:
2935 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5094 5492 5766 5913 6324 5094
Size: 398 274 147 411 5431
Fragments arranged by size:
5431 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3311 3341 1178
Size: 30 165 30 1908 30 4498
Fragments arranged by size:
4498 1908 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2892 3332 3491 1186
Size: 1706 440 159 4356
Fragments arranged by size:
4356 1706 440 159
BseYI C'CCAG_C
Cuts at: 2033 5864 6360 2033
Size: 3831 496 2334
Fragments arranged by size:
3831 2334 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2187 2256 2523 2841 3875 1163
Size: 573 451 69 267 318 1034 3949
Fragments arranged by size:
3949 1034 573 451 318 267 69
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4111 4872 5900 1540
Size: 275 186 2110 761 1028 2301
Fragments arranged by size:
2301 2110 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4722 4807 5878 4722
Size: 85 1071 5505
Fragments arranged by size:
5505 1071 85
BsmI GAATG_Cn'
Cuts at: 4172 4265 4172
Size: 93 6568
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3441 3980 4722 4807 5878 1391
Size: 399 1651 539 742 85 1071 2174
Fragments arranged by size:
2174 1651 1071 742 539 399 85
BspEI T'CCGG_A
Cuts at: 6324 6324
Size: 6661
BspHI T'CATG_A
Cuts at: 2181 4550 6280 2181
Size: 2369 1730 2562
Fragments arranged by size:
2562 2369 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4016 844
Size: 3172 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4548 1394
Size: 429 2725 3507
Fragments arranged by size:
3507 2725 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2328 2488 5156 5330 65
Size: 1183 1080 160 2668 174 1396
Fragments arranged by size:
2668 1396 1183 1080 174 160
BsrFI r'CCGG_y
Cuts at: 1700 1810 5305 5492 1700
Size: 110 3495 187 2869
Fragments arranged by size:
3495 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6661
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6661
BssSI C'ACGA_G
Cuts at: 4721 5733 4721
Size: 1012 5649
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6661
BstBI TT'CG_AA
Cuts at: 2007 5465 2007
Size: 3458 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6661
BstYI r'GATC_y
Cuts at: 1972 2765 3445 3991 4358 4743 4760 6212 6298
Size: 793 680 546 367 385 17 1452 86
Cuts at: 6298 6310 6656 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 793 680 546 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5152
Fragments arranged by size:
5152 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5922
Fragments arranged by size:
5922 683 56
BtsI GCAGTG_nn'
Cuts at: 4177 4971 4991 4177
Size: 794 20 5847
Fragments arranged by size:
5847 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6661
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6626
DraI TTT'AAA
Cuts at: 2314 3604 3965 2314
Size: 1290 361 5010
Fragments arranged by size:
5010 1290 361
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5668 809
Size: 507 4352 1802
Fragments arranged by size:
4352 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3640 3915 4108
Size: 54 1425 50 275 18 1810 275 193
Cuts at: 4108 4997 6626 8
Size: 889 1629 43
Fragments arranged by size:
1810 1629 1425 889 275 275 193 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4108 1537
Size: 275 2296 4090
Fragments arranged by size:
4090 2296 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2410 2547 2958 3114 3489 4591 1353
Size: 1057 137 411 156 375 1102 3423
Fragments arranged by size:
3423 1102 1057 411 375 156 137
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2677 5248 5622 5768 387
Size: 1064 138 1088 2571 374 146 1280
Fragments arranged by size:
2571 1280 1088 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2281 3138 3395 4724 5321 6108
Size: 393 407 242 857 257 1329 597 787
Cuts at: 6108 6376 1239
Size: 268 1524
Fragments arranged by size:
1524 1329 857 787 597 407 393 268
257 242
EcoO109I rG'GnC_Cy
Cuts at: 3240 3322 3932 3240
Size: 82 610 5969
Fragments arranged by size:
5969 610 82
EcoRI G'AATT_C
Cuts at: 3970 4348 3970
Size: 378 6283
FspI TGC'GCA
Cuts at: 5167 5167
Size: 6661
HaeII r_GCGC'y
Cuts at: 1678 5808 1678
Size: 4130 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2540 2572 3311 3314 3343
Size: 32 135 32 968 32 739 3 29
Cuts at: 3343 3346 3497 3529 5302 5334 5376 5408 1373
Size: 3 151 32 1773 32 42 32 2626
Fragments arranged by size:
2626 1773 968 739 151 135 42 32
32 32 32 32 32 29 3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 4005 4251 669
Size: 988 281 2067 246 3079
Fragments arranged by size:
3079 2067 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6661
HpaI GTT'AAC
Cuts at: 4251 4251
Size: 6661
KpnI G_GTAC'C
Cuts at: 3986 3986
Size: 6661
MfeI C'AATT_G
Cuts at: 2109 4260 2109
Size: 2151 4510
MluI A'CGCG_T
Cuts at: 6537 6537
Size: 6661
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5775 5959 1305
Size: 4470 184 2007
Fragments arranged by size:
4470 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3642 3917 10
Size: 54 1425 343 1810 275 2754
Fragments arranged by size:
2754 1810 1425 343 275 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4619 4978 5137 518
Size: 648 3453 359 159 2042
Fragments arranged by size:
3453 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3790 4077 4754 5902 6147 1606
Size: 2184 287 677 1148 245 2120
Fragments arranged by size:
2184 2120 1148 677 287 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5152
Fragments arranged by size:
5152 957 552
NdeI CA'TA_TG
Cuts at: 387 3678 387
Size: 3291 3370
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6661
NotI GC'GGCC_GC
Cuts at: 4108 4108
Size: 6661
NsiI A_TGCA'T
Cuts at: 3286 3286
Size: 6661
NspI r_CATG'y
Cuts at: 1132 1229 2726 3372 3422 3855 4019 5564 1132
Size: 97 1497 646 50 433 164 1545 2229
Fragments arranged by size:
2229 1545 1497 646 433 164 97 50
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6661
PciI A'CATG_T
Cuts at: 3851 5560 3851
Size: 1709 4952
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2496 1108
Size: 1388 5273
PfoI T'CCnGG_A
Cuts at: 2370 2370
Size: 6661
PmeI GTTT'AAAC
Cuts at: 3965 3965
Size: 6661
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4730 4762 6243 6275 4730
Size: 32 1481 32 5116
Fragments arranged by size:
5116 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3322 3322
Size: 6661
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6661
PsiI TTA'TAA
Cuts at: 4231 4231
Size: 6661
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2593 2625 2593
Size: 32 6629
PstI C_TGCA'G
Cuts at: 830 4013 830
Size: 3183 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6661
SalI G'TCGA_C
Cuts at: 1936 4003 1936
Size: 2067 4594
SapI GCTCTTCn'nnn_
Cuts at: 3114 3114
Size: 6661
SbfI CC_TGCA'GG
Cuts at: 4013 4013
Size: 6661
ScaI AGT'ACT
Cuts at: 2200 4909 6621 2200
Size: 2709 1712 2240
Fragments arranged by size:
2709 2240 1712
SfcI C'TryA_G
Cuts at: 826 1045 2155 2333 3027 4009 5144 5825 6016
Size: 219 1110 178 694 982 1135 681 191
Cuts at: 6016 826
Size: 1471
Fragments arranged by size:
1471 1135 1110 982 694 681 219 191
178
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6661
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6661
SmaI CCC'GGG
Cuts at: 1872 3988 1872
Size: 2116 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3799 4765 5666 5928 820
Size: 197 926 1856 966 901 262 1553
Fragments arranged by size:
1856 1553 966 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3958 4452 493
Size: 3465 494 2702
Fragments arranged by size:
3465 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6661
SphI G_CATG'C
Cuts at: 1132 4019 1132
Size: 2887 3774
SspI AAT'ATT
Cuts at: 5 52 4585 5
Size: 47 4533 2081
Fragments arranged by size:
4533 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6661
StyI C'CwwG_G
Cuts at: 513 1065 2022 4100 513
Size: 552 957 2078 3074
Fragments arranged by size:
3074 2078 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4688 4705 4858 5043 5488 325
Size: 1255 3108 17 153 185 445 1498
Fragments arranged by size:
3108 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2198 2735 3262
Size: 274 80 33 51 1443 221 537 527
Cuts at: 3262 4907 6619 96
Size: 1645 1712 138
Fragments arranged by size:
1712 1645 1443 537 527 274 221 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6621 6653 6621
Size: 32 6629
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6661
XbaI T'CTAG_A
Cuts at: 3997 3997
Size: 6661
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6661
XmaI C'CCGG_G
Cuts at: 1870 3986 1870
Size: 2116 4545
XmnI GAAnn'nnTTC
Cuts at: 3021 4790 3021
Size: 1769 4892
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4469 276
Size: 53 83 186 936 2935 2468
Fragments arranged by size:
2935 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PspOMI PspXI PvuII
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI