Restriction map for
pFN21ASDA0992
(Circular) (Six-base) MAPSORT of: pFN21ASDA0992.seq Check: 3480 from: 1 to: 6745
pFN21ASDA0992 6745 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4555 278
Size: 53 83 186 936 3019 2468
Fragments arranged by size:
3019 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2692 3806 4088 1937
Size: 755 1114 282 4594
Fragments arranged by size:
4594 1114 755 282
Acc65I G'GTAC_C
Cuts at: 3633 4066 3633
Size: 433 6312
AclI AA'CG_TT
Cuts at: 1563 4872 5245 1563
Size: 3309 373 3063
Fragments arranged by size:
3309 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2293 3271 4808 6192 6460 1632
Size: 661 978 1537 1384 268 1917
Fragments arranged by size:
1917 1537 1384 978 661 268
AfeI AGC'GCT
Cuts at: 2455 2455
Size: 6745
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6548
AflIII A'CryG_T
Cuts at: 1203 2340 2951 3509 5644 6621 1203
Size: 1137 611 558 2135 977 1327
Fragments arranged by size:
2135 1327 1137 977 611 558
AgeI A'CCGG_T
Cuts at: 5576 5576
Size: 6745
AhdI GACnn_n'nnGTC
Cuts at: 5474 5474
Size: 6745
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6745
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6745
AlwNI CAG_nnn'CTG
Cuts at: 4488 6060 4488
Size: 1572 5173
ApaI G_GGCC'C
Cuts at: 2124 2124
Size: 6745
ApaLI G'TGCA_C
Cuts at: 3458 4802 5958 3458
Size: 1344 1156 4245
Fragments arranged by size:
4245 1344 1156
ApoI r'AATT_y
Cuts at: 1493 2577 4054 4280 4432 6541 1493
Size: 1084 1477 226 152 2109 1697
Fragments arranged by size:
2109 1697 1477 1084 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5299 160
Size: 872 4267 1606
Fragments arranged by size:
4267 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6745
AvaI C'yCGr_G
Cuts at: 1870 1943 2117 2252 2374 2380 4070 1870
Size: 73 174 135 122 6 1690 4545
Fragments arranged by size:
4545 1690 174 135 122 73 6
BamHI G'GATC_C
Cuts at: 4075 4442 4075
Size: 367 6378
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2332 2887 3633 4066 5521
Size: 325 217 627 545 555 746 433 1455
Cuts at: 5521 618
Size: 1842
Fragments arranged by size:
1842 1455 746 627 555 545 433 325
217
BanII G_rGCy'C
Cuts at: 1391 2124 2253 2270 2381 4064 1391
Size: 733 129 17 111 1683 4072
Fragments arranged by size:
4072 1683 733 129 111 17
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2299 928
Size: 971 400 5374
Fragments arranged by size:
5374 971 400
BbvCI CC'TCA_GC
Cuts at: 2450 3919 2450
Size: 1469 5276
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3924 3958 4936 4970 1903
Size: 34 1987 34 978 34 3678
Fragments arranged by size:
3678 1987 978 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4637 5847 4637
Size: 1210 5535
BclI T'GATC_A
Cuts at: 1548 3025 3468 1548
Size: 1477 443 4825
Fragments arranged by size:
4825 1477 443
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5356 136
Size: 107 122 71 631 605 3684 1525
Fragments arranged by size:
3684 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2922 2982 3009 3046 3316 3382 6740 2922
Size: 60 27 37 270 66 3358 2927
Fragments arranged by size:
3358 2927 270 66 60 37 27
BlpI GC'TnA_GC
Cuts at: 2247 4162 2247
Size: 1915 4830
Bme1580I G_kGCm'C
Cuts at: 1790 2124 2337 3462 4806 5962 1790
Size: 334 213 1125 1344 1156 2573
Fragments arranged by size:
2573 1344 1156 1125 334 213
BmgBI CAC'GTC
Cuts at: 1318 2341 3486 1318
Size: 1023 1145 4577
Fragments arranged by size:
4577 1145 1023
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3632 3865 4476
Size: 416 53 83 399 24 2215 233 611
Cuts at: 4476 5434 442
Size: 958 1753
Fragments arranged by size:
2215 1753 958 611 416 399 233 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6745
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2134 2758 3333 3572 5405 1239
Size: 895 624 575 239 1833 2579
Fragments arranged by size:
2579 1833 895 624 575 239
Bpu10I CC'TnA_GC
Cuts at: 2450 3919 2450
Size: 1469 5276
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4870 5735 6033 4870
Size: 865 298 5582
Fragments arranged by size:
5582 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2829 3204 5408 882
Size: 617 1330 375 2204 2219
Fragments arranged by size:
2219 2204 1330 617 375
BsaAI yAC'GTr
Cuts at: 493 1204 3491 4042 4536 493
Size: 711 2287 551 494 2702
Fragments arranged by size:
2702 2287 711 551 494
BsaBI GATnn'nnATC
Cuts at: 6739 6739
Size: 6745
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2192 2390 2435 4552
Size: 53 83 186 936 659 198 45 2117
Cuts at: 4552 4934 5569 275
Size: 382 635 1451
Fragments arranged by size:
2117 1451 936 659 635 382 198 186
83 53 45
BsaWI w'CCGG_w
Cuts at: 5178 5576 5850 5997 6408 5178
Size: 398 274 147 411 5515
Fragments arranged by size:
5515 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2355 2385 2756 2786 1178
Size: 30 165 30 952 30 371 30 5137
Fragments arranged by size:
5137 952 371 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2260 2263 1186
Size: 1074 3 5668
Fragments arranged by size:
5668 1074 3
BseYI C'CCAG_C
Cuts at: 2156 3797 5948 6444 2156
Size: 1641 2151 496 2457
Fragments arranged by size:
2457 2151 1641 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6172
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2209 2487 4195 4956 5984 1540
Size: 275 186 208 278 1708 761 1028 2301
Fragments arranged by size:
2301 1708 1028 761 278 275 208 186
BsiHKAI G_wGCw'C
Cuts at: 3430 3462 3896 4806 4891 5962 3430
Size: 32 434 910 85 1071 4213
Fragments arranged by size:
4213 1071 910 434 85 32
BsiWI C'GTAC_G
Cuts at: 3412 3412
Size: 6745
BsmI GAATG_Cn'
Cuts at: 2940 4256 4349 2940
Size: 1316 93 5336
Fragments arranged by size:
5336 1316 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3249 3249
Size: 6745
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2124 2253 2270 2337 2381 3430 3462
Size: 399 334 129 17 67 44 1049 32
Cuts at: 3462 3896 4064 4806 4891 5962 1391
Size: 434 168 742 85 1071 2174
Fragments arranged by size:
2174 1071 1049 742 434 399 334 168
129 85 67 44 32 17
BspEI T'CCGG_A
Cuts at: 6408 6408
Size: 6745
BspHI T'CATG_A
Cuts at: 4634 6364 4634
Size: 1730 5015
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3068 4100 844
Size: 2224 1032 3489
Fragments arranged by size:
3489 2224 1032
BsrBI CCG'CTC
Cuts at: 1394 1823 2043 2371 4632 1394
Size: 429 220 328 2261 3507
Fragments arranged by size:
3507 2261 429 328 220
BsrDI GCAATG_nn'
Cuts at: 65 1248 5240 5414 65
Size: 1183 3992 174 1396
Fragments arranged by size:
3992 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2143 2479 3497 5389 5576 1700
Size: 110 333 336 1018 1892 187 2869
Fragments arranged by size:
2869 1892 1018 336 333 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6745
BssHII G'CGCG_C
Cuts at: 1925 2028 2084 1925
Size: 103 56 6586
Fragments arranged by size:
6586 103 56
BssSI C'ACGA_G
Cuts at: 4805 5817 4805
Size: 1012 5733
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6745
BstBI TT'CG_AA
Cuts at: 2007 5549 2007
Size: 3542 3203
BstEII G'GTnAC_C
Cuts at: 1184 3369 1184
Size: 2185 4560
BstYI r'GATC_y
Cuts at: 1972 2922 2982 3009 3046 3316 3382 4075 4442
Size: 950 60 27 37 270 66 693 367
Cuts at: 4442 4827 4844 6296 6382 6394 6740 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 950 693 385 367 346 270
86 66 60 37 27 17 12
BstZ17I GTA'TAC
Cuts at: 3807 3807
Size: 6745
Bsu36I CC'TnA_GG
Cuts at: 3129 3205 3981 3129
Size: 76 776 5893
Fragments arranged by size:
5893 776 76
BtgI C'CryG_G
Cuts at: 513 1065 2134 2261 2316 2425 2439 3751 513
Size: 552 1069 127 55 109 14 1312 3507
Fragments arranged by size:
3507 1312 1069 552 127 109 55 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2086 487
Size: 683 56 860 5146
Fragments arranged by size:
5146 860 683 56
BtsI GCAGTG_nn'
Cuts at: 2348 2395 4261 5055 5075 2348
Size: 47 1866 794 20 4018
Fragments arranged by size:
4018 1866 794 47 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6745
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3346 3381 529
Size: 35 2782 35 3893
Fragments arranged by size:
3893 2782 35 35
DraI TTT'AAA
Cuts at: 2995 4049 2995
Size: 1054 5691
DraIII CAC_nnn'GTG
Cuts at: 3873 3873
Size: 6745
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3359 5752 809
Size: 507 2043 2393 1802
Fragments arranged by size:
2393 2043 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2206 2422 2428
Size: 54 1425 50 275 18 376 216 6
Cuts at: 2428 2484 2653 4192 5081 6710 8
Size: 56 169 1539 889 1629 43
Fragments arranged by size:
1629 1539 1425 889 376 275 216 169
56 54 50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2206 2484 4192 1537
Size: 275 394 278 1708 4090
Fragments arranged by size:
4090 1708 394 278 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2736 4675 1353
Size: 1383 1939 3423
Fragments arranged by size:
3423 1939 1383
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2035 5332 5706 5852 387
Size: 1064 138 446 3297 374 146 1280
Fragments arranged by size:
3297 1280 1064 446 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2134 2293 2758 3271 3333 3572 4808
Size: 393 502 159 465 513 62 239 1236
Cuts at: 4808 5405 6192 6460 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1236 787 597 513 502 465 393
268 239 159 62
EcoO109I rG'GnC_Cy
Cuts at: 2257 2638 3057 4033 2257
Size: 381 419 976 4969
Fragments arranged by size:
4969 976 419 381
EcoRI G'AATT_C
Cuts at: 4054 4432 4054
Size: 378 6367
FspI TGC'GCA
Cuts at: 5251 5251
Size: 6745
HaeII r_GCGC'y
Cuts at: 1678 2247 2457 5892 1678
Size: 569 210 3435 2531
Fragments arranged by size:
3435 2531 569 210
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3945 3977 5386 5418 5460
Size: 32 135 32 2373 32 1409 32 42
Cuts at: 5460 5492 1373
Size: 32 2626
Fragments arranged by size:
2626 2373 1409 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2491 2667 2693 3175 4089 4335
Size: 988 281 553 176 26 482 914 246
Cuts at: 4335 669
Size: 3079
Fragments arranged by size:
3079 988 914 553 482 281 246 176
26
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6745
HpaI GTT'AAC
Cuts at: 2667 4335 2667
Size: 1668 5077
KpnI G_GTAC'C
Cuts at: 3637 4070 3637
Size: 433 6312
MfeI C'AATT_G
Cuts at: 4344 4344
Size: 6745
MluI A'CGCG_T
Cuts at: 6621 6621
Size: 6745
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4000 5859 6043 1305
Size: 2695 1859 184 2007
Fragments arranged by size:
2695 2007 1859 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2655 10
Size: 54 1425 343 823 4100
Fragments arranged by size:
4100 1425 823 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3216 4703 5062 5221 518
Size: 648 2050 1487 359 159 2042
Fragments arranged by size:
2050 2042 1487 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2441 2778 4161 4838 5986 6231 1606
Size: 835 337 1383 677 1148 245 2120
Fragments arranged by size:
2120 1383 1148 835 677 337 245
NaeI GCC'GGC
Cuts at: 2145 2481 3499 2145
Size: 336 1018 5391
Fragments arranged by size:
5391 1018 336
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6193
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6745
NgoMIV G'CCGG_C
Cuts at: 2143 2479 3497 2143
Size: 336 1018 5391
Fragments arranged by size:
5391 1018 336
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6745
NotI GC'GGCC_GC
Cuts at: 4192 4192
Size: 6745
NspI r_CATG'y
Cuts at: 1132 1229 2955 3513 3739 4103 5648 1132
Size: 97 1726 558 226 364 1545 2229
Fragments arranged by size:
2229 1726 1545 558 364 226 97
PasI CC'CwG_GG
Cuts at: 1348 2497 1348
Size: 1149 5596
PciI A'CATG_T
Cuts at: 2951 3509 5644 2951
Size: 558 2135 4052
Fragments arranged by size:
4052 2135 558
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6745
PfoI T'CCnGG_A
Cuts at: 3310 3310
Size: 6745
PmeI GTTT'AAAC
Cuts at: 4049 4049
Size: 6745
PmlI CAC'GTG
Cuts at: 3491 3491
Size: 6745
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2775 2807 4814 4846 6327 6359 2775
Size: 32 2007 32 1481 32 3161
Fragments arranged by size:
3161 2007 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2638 3057 4033 2638
Size: 419 976 5350
Fragments arranged by size:
5350 976 419
PshAI GACnn'nnGTC
Cuts at: 1613 2389 1613
Size: 776 5969
PsiI TTA'TAA
Cuts at: 4315 4315
Size: 6745
PspOMI G'GGCC_C
Cuts at: 2120 2120
Size: 6745
PstI C_TGCA'G
Cuts at: 830 3347 4097 830
Size: 2517 750 3478
Fragments arranged by size:
3478 2517 750
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6745
PvuII CAG'CTG
Cuts at: 2778 2778
Size: 6745
SacII CC_GC'GG
Cuts at: 2442 2442
Size: 6745
SalI G'TCGA_C
Cuts at: 1936 2691 4087 1936
Size: 755 1396 4594
Fragments arranged by size:
4594 1396 755
SbfI CC_TGCA'GG
Cuts at: 4097 4097
Size: 6745
ScaI AGT'ACT
Cuts at: 4993 6705 4993
Size: 1712 5033
SfcI C'TryA_G
Cuts at: 826 1045 3343 4093 5228 5909 6100 826
Size: 219 2298 750 1135 681 191 1471
Fragments arranged by size:
2298 1471 1135 750 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6745
SgrAI Cr'CCGG_yG
Cuts at: 1700 2479 1700
Size: 779 5966
SmaI CCC'GGG
Cuts at: 1872 2382 4072 1872
Size: 510 1690 4545
Fragments arranged by size:
4545 1690 510
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4849 5750 6012 820
Size: 197 926 2906 901 262 1553
Fragments arranged by size:
2906 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4042 4536 493
Size: 3549 494 2702
Fragments arranged by size:
3549 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6745
SphI G_CATG'C
Cuts at: 1132 3739 4103 1132
Size: 2607 364 3774
Fragments arranged by size:
3774 2607 364
SspI AAT'ATT
Cuts at: 5 52 2728 4669 5
Size: 47 2676 1941 2081
Fragments arranged by size:
2676 2081 1941 47
StuI AGG'CCT
Cuts at: 1517 3229 1517
Size: 1712 5033
StyI C'CwwG_G
Cuts at: 513 1065 2508 4017 4184 513
Size: 552 1443 1509 167 3074
Fragments arranged by size:
3074 1509 1443 552 167
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3741 3789 3884 4772 4789 4942 5127
Size: 1255 2161 48 95 888 17 153 185
Cuts at: 5127 5572 325
Size: 445 1498
Fragments arranged by size:
2161 1498 1255 888 445 185 153 95
48 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2808 3146 3845
Size: 274 80 33 51 1443 831 338 699
Cuts at: 3845 4991 6703 96
Size: 1146 1712 138
Fragments arranged by size:
1712 1443 1146 831 699 338 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2355 2387 6705 6737 2355
Size: 32 4318 32 2363
Fragments arranged by size:
4318 2363 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6745
XbaI T'CTAG_A
Cuts at: 3236 4081 3236
Size: 845 5900
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2029 2985 3387 2029
Size: 956 402 5387
Fragments arranged by size:
5387 956 402
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6745
XmaI C'CCGG_G
Cuts at: 1870 2380 4070 1870
Size: 510 1690 4545
Fragments arranged by size:
4545 1690 510
XmnI GAAnn'nnTTC
Cuts at: 3270 3542 4874 3270
Size: 272 1332 5141
Fragments arranged by size:
5141 1332 272
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4553 276
Size: 53 83 186 936 3019 2468
Fragments arranged by size:
3019 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BplI
BstXI EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PspXI PsrI RsrII SacI
SanDI SapI SexAI SfiI SfoI SrfI SwaI