Restriction map for
pFN21ASDA0990
(Circular) (Six-base) MAPSORT of: pFN21ASDA0990.seq Check: 3385 from: 1 to: 7135
pFN21ASDA0990 7135 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3918 4945 278
Size: 53 83 186 936 2382 1027 2468
Fragments arranged by size:
2468 2382 1027 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3918 4478 1937
Size: 1981 560 4594
Fragments arranged by size:
4594 1981 560
Acc65I G'GTAC_C
Cuts at: 4456 4456
Size: 7135
AclI AA'CG_TT
Cuts at: 1563 5262 5635 1563
Size: 3699 373 3063
Fragments arranged by size:
3699 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2144 3575 3929 3985 5198 6582 6850 1632
Size: 512 1431 354 56 1213 1384 268 1917
Fragments arranged by size:
1917 1431 1384 1213 512 354 268 56
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6938
AflIII A'CryG_T
Cuts at: 1203 2541 3432 3657 3692 4109 6034 7011 1203
Size: 1338 891 225 35 417 1925 977 1327
Fragments arranged by size:
1925 1338 1327 977 891 417 225 35
AgeI A'CCGG_T
Cuts at: 5966 5966
Size: 7135
AhdI GACnn_n'nnGTC
Cuts at: 5864 5864
Size: 7135
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7135
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7135
AlwNI CAG_nnn'CTG
Cuts at: 4351 4878 6450 4351
Size: 527 1572 5036
Fragments arranged by size:
5036 1572 527
ApaI G_GGCC'C
Cuts at: 2138 2138
Size: 7135
ApaLI G'TGCA_C
Cuts at: 5192 6348 5192
Size: 1156 5979
ApoI r'AATT_y
Cuts at: 1493 2910 3066 3938 4444 4670 4822 6931 1493
Size: 1417 156 872 506 226 152 2109 1697
Fragments arranged by size:
2109 1697 1417 872 506 226 156 152
AscI GG'CGCG_CC
Cuts at: 2041 2041
Size: 7135
AseI AT'TA_AT
Cuts at: 160 1032 5689 160
Size: 872 4657 1606
Fragments arranged by size:
4657 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7135
AvaI C'yCGr_G
Cuts at: 1870 1943 2063 2109 2156 2322 4460 1870
Size: 73 120 46 47 166 2138 4545
Fragments arranged by size:
4545 2138 166 120 73 47 46
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2391 2424 2391
Size: 33 7102
BamHI G'GATC_C
Cuts at: 3527 3872 4465 4832 3527
Size: 345 593 367 5830
Fragments arranged by size:
5830 593 367 345
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2142 2627 2728 4456 5911
Size: 325 217 627 355 485 101 1728 1455
Cuts at: 5911 618
Size: 1842
Fragments arranged by size:
1842 1728 1455 627 485 355 325 217
101
BanII G_rGCy'C
Cuts at: 1391 2070 2138 2601 3003 3856 4454 1391
Size: 679 68 463 402 853 598 4072
Fragments arranged by size:
4072 853 679 598 463 402 68
BbeI G_GCGC'C
Cuts at: 2146 2146
Size: 7135
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3695 4226 928
Size: 971 1796 531 3837
Fragments arranged by size:
3837 1796 971 531
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5326 5360 1903
Size: 34 3389 34 3678
Fragments arranged by size:
3678 3389 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5027 6237 5027
Size: 1210 5925
BclI T'GATC_A
Cuts at: 1548 2697 3267 1548
Size: 1149 570 5416
Fragments arranged by size:
5416 1149 570
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2160 2201 5746
Size: 107 122 71 631 605 488 41 3545
Cuts at: 5746 136
Size: 1525
Fragments arranged by size:
3545 1525 631 605 488 122 107 71
41
BglII A'GATC_T
Cuts at: 2893 4126 7130 2893
Size: 1233 3004 2898
Fragments arranged by size:
3004 2898 1233
BlpI GC'TnA_GC
Cuts at: 2051 2981 4552 2051
Size: 930 1571 4634
Fragments arranged by size:
4634 1571 930
Bme1580I G_kGCm'C
Cuts at: 1790 2138 2630 5196 6352 1790
Size: 348 492 2566 1156 2573
Fragments arranged by size:
2573 2566 1156 492 348
BmgBI CAC'GTC
Cuts at: 1318 2542 2779 3693 4255 1318
Size: 1224 237 914 562 4198
Fragments arranged by size:
4198 1224 914 562 237
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3874 4038 4866
Size: 416 53 83 399 24 2457 164 828
Cuts at: 4866 5824 442
Size: 958 1753
Fragments arranged by size:
2457 1753 958 828 416 399 164 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7135
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2585 2678 3068 3858 4105 4196 5795 1239
Size: 1346 93 390 790 247 91 1599 2579
Fragments arranged by size:
2579 1599 1346 790 390 247 93 91
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2368 2465 5260 6125 6423 2368
Size: 97 2795 865 298 3080
Fragments arranged by size:
3080 2795 865 298 97
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2550 5798 882
Size: 617 1051 3248 2219
Fragments arranged by size:
3248 2219 1051 617
BsaAI yAC'GTr
Cuts at: 493 1204 4110 4432 4926 493
Size: 711 2906 322 494 2702
Fragments arranged by size:
2906 2702 711 494 322
BsaBI GATnn'nnATC
Cuts at: 4281 7129 4281
Size: 2848 4287
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2143 3915 4942 5324
Size: 53 83 186 936 610 1772 1027 382
Cuts at: 5324 5959 275
Size: 635 1451
Fragments arranged by size:
1772 1451 1027 936 635 610 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2799 5568 5966 6240 6387 6798 2799
Size: 2769 398 274 147 411 3136
Fragments arranged by size:
3136 2769 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4116 4146 1178
Size: 30 165 30 2713 30 4167
Fragments arranged by size:
4167 2713 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2593 3149 3439 3494 1186
Size: 1407 556 290 55 4827
Fragments arranged by size:
4827 1407 556 290 55
BseYI C'CCAG_C
Cuts at: 3088 4167 6338 6834 3088
Size: 1079 2171 496 3389
Fragments arranged by size:
3389 2171 1079 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2837 3008 1163
Size: 573 1101 171 5290
Fragments arranged by size:
5290 1101 573 171
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2038 4585 5346 6374 1540
Size: 275 186 37 2547 761 1028 2301
Fragments arranged by size:
2547 2301 1028 761 275 186 37
BsiHKAI G_wGCw'C
Cuts at: 3003 3600 5196 5281 6352 3003
Size: 597 1596 85 1071 3786
Fragments arranged by size:
3786 1596 1071 597 85
BsmI GAATG_Cn'
Cuts at: 4646 4739 4646
Size: 93 7042
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2138 2601 2630 3003 3600 3856
Size: 399 280 68 463 29 373 597 256
Cuts at: 3856 4454 5196 5281 6352 1391
Size: 598 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 598 597 463 399 373
280 256 85 68 29
BspEI T'CCGG_A
Cuts at: 2799 6798 2799
Size: 3999 3136
BspHI T'CATG_A
Cuts at: 2295 2472 2700 3108 5024 6754 2295
Size: 177 228 408 1916 1730 2676
Fragments arranged by size:
2676 1916 1730 408 228 177
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2322 3244 3381 4202 4490 844
Size: 1478 922 137 821 288 3489
Fragments arranged by size:
3489 1478 922 821 288 137
BsrBI CCG'CTC
Cuts at: 1394 1823 2132 5022 1394
Size: 429 309 2890 3507
Fragments arranged by size:
3507 2890 429 309
BsrDI GCAATG_nn'
Cuts at: 65 1248 5630 5804 65
Size: 1183 4382 174 1396
Fragments arranged by size:
4382 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2037 2166 2195 5779 5966 1700
Size: 110 227 129 29 3584 187 2869
Fragments arranged by size:
3584 2869 227 187 129 110 29
BsrGI T'GTAC_A
Cuts at: 96 2544 2760 3384 96
Size: 2448 216 624 3847
Fragments arranged by size:
3847 2448 624 216
BssHII G'CGCG_C
Cuts at: 1925 2030 2041 2204 2216 1925
Size: 105 11 163 12 6844
Fragments arranged by size:
6844 163 105 12 11
BssSI C'ACGA_G
Cuts at: 3722 5195 6207 3722
Size: 1473 1012 4650
Fragments arranged by size:
4650 1473 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3826 794
Size: 3032 4103
BstBI TT'CG_AA
Cuts at: 2007 5939 2007
Size: 3932 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7135
BstYI r'GATC_y
Cuts at: 1972 2893 3315 3527 3872 4126 4465 4832 5217
Size: 921 422 212 345 254 339 367 385
Cuts at: 5217 5234 6686 6772 6784 7130 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 921 422 385 367 346 345
339 254 212 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2576 2576
Size: 7135
BtgI C'CryG_G
Cuts at: 513 1065 2022 2138 2328 2631 513
Size: 552 957 116 190 303 5017
Fragments arranged by size:
5017 957 552 303 190 116
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2226 2993 3964 487
Size: 683 56 1000 767 971 3658
Fragments arranged by size:
3658 1000 971 767 683 56
BtsI GCAGTG_nn'
Cuts at: 2825 4651 5445 5465 2825
Size: 1826 794 20 4495
Fragments arranged by size:
4495 1826 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7135
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7100
DraI TTT'AAA
Cuts at: 4439 4439
Size: 7135
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3245 4336 6142 809
Size: 507 1929 1091 1806 1802
Fragments arranged by size:
1929 1806 1802 1091 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2035 2234
Size: 54 1425 50 275 18 195 10 199
Cuts at: 2234 2331 3190 3501 4582 5471 7100 8
Size: 97 859 311 1081 889 1629 43
Fragments arranged by size:
1629 1425 1081 889 859 311 275 199
195 97 54 50 43 18 10
EagI C'GGCC_G
Cuts at: 1537 1812 2035 4582 1537
Size: 275 223 2547 4090
Fragments arranged by size:
4090 2547 275 223
EarI CTCTTCn'nnn_
Cuts at: 1353 2288 3199 3584 3908 5065 1353
Size: 935 911 385 324 1157 3423
Fragments arranged by size:
3423 1157 935 911 385 324
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2993 3208 5722 6096 6242 387
Size: 1064 138 1404 215 2514 374 146 1280
Fragments arranged by size:
2514 1404 1280 1064 374 215 146 138
EcoICRI GAG'CTC
Cuts at: 3001 3001
Size: 7135
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2144 2585 2678 3068 3575 3858 3929
Size: 393 512 441 93 390 507 283 71
Cuts at: 3929 3985 4105 4196 5198 5795 6582 6850 1239
Size: 56 120 91 1002 597 787 268 1524
Fragments arranged by size:
1524 1002 787 597 512 507 441 393
390 283 268 120 93 91 71 56
EcoNI CCTnn'n_nnAGG
Cuts at: 3109 3204 3109
Size: 95 7040
EcoO109I rG'GnC_Cy
Cuts at: 2687 3079 2687
Size: 392 6743
EcoRI G'AATT_C
Cuts at: 3938 4444 4822 3938
Size: 506 378 6251
Fragments arranged by size:
6251 506 378
EcoRV GAT'ATC
Cuts at: 2994 2994
Size: 7135
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2485 2485
Size: 7135
FspI TGC'GCA
Cuts at: 5641 5641
Size: 7135
HaeII r_GCGC'y
Cuts at: 1678 2146 6282 1678
Size: 468 4136 2531
Fragments arranged by size:
4136 2531 468
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3282 3314 3499 3531 4116
Size: 32 135 32 1710 32 185 32 585
Cuts at: 4116 4148 5776 5808 5850 5882 1373
Size: 32 1628 32 42 32 2626
Fragments arranged by size:
2626 1710 1628 585 185 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3187 3919 4479 4725 669
Size: 988 281 1249 732 560 246 3079
Fragments arranged by size:
3079 1249 988 732 560 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7135
HpaI GTT'AAC
Cuts at: 4725 4725
Size: 7135
KasI G'GCGC_C
Cuts at: 2142 2142
Size: 7135
KpnI G_GTAC'C
Cuts at: 4460 4460
Size: 7135
MfeI C'AATT_G
Cuts at: 4734 4734
Size: 7135
MluI A'CGCG_T
Cuts at: 3432 7011 3432
Size: 3579 3556
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3774 6249 6433 1305
Size: 2469 2475 184 2007
Fragments arranged by size:
2475 2469 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3503 10
Size: 54 1425 343 1671 3642
Fragments arranged by size:
3642 1671 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3248 3263 3662 5093 5452 5611 518
Size: 648 2082 15 399 1431 359 159 2042
Fragments arranged by size:
2082 2042 1431 648 399 359 159 15
MspA1I CmG'CkG
Cuts at: 1606 3022 3088 4351 4551 5228 6376 6621 1606
Size: 1416 66 1263 200 677 1148 245 2120
Fragments arranged by size:
2120 1416 1263 1148 677 245 200 66
NaeI GCC'GGC
Cuts at: 2039 2197 2039
Size: 158 6977
NarI GG'CG_CC
Cuts at: 2143 2143
Size: 7135
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5626
Fragments arranged by size:
5626 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7135
NgoMIV G'CCGG_C
Cuts at: 2037 2195 2037
Size: 158 6977
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7135
NotI GC'GGCC_GC
Cuts at: 4582 4582
Size: 7135
NspI r_CATG'y
Cuts at: 1132 1229 2491 2980 3661 3820 4493 6038 1132
Size: 97 1262 489 681 159 673 1545 2229
Fragments arranged by size:
2229 1545 1262 681 673 489 159 97
PasI CC'CwG_GG
Cuts at: 1348 3581 1348
Size: 2233 4902
PciI A'CATG_T
Cuts at: 3657 6034 3657
Size: 2377 4758
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2503 3469 1108
Size: 1395 966 4774
Fragments arranged by size:
4774 1395 966
PfoI T'CCnGG_A
Cuts at: 2751 3366 2751
Size: 615 6520
PmeI GTTT'AAAC
Cuts at: 4439 4439
Size: 7135
PmlI CAC'GTG
Cuts at: 4110 4110
Size: 7135
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5204 5236 6717 6749 5204
Size: 32 1481 32 5590
Fragments arranged by size:
5590 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3079 3079
Size: 7135
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7135
PsiI TTA'TAA
Cuts at: 4119 4705 4119
Size: 586 6549
PspOMI G'GGCC_C
Cuts at: 2134 2134
Size: 7135
PstI C_TGCA'G
Cuts at: 830 2319 4487 830
Size: 1489 2168 3478
Fragments arranged by size:
3478 2168 1489
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7135
PvuII CAG'CTG
Cuts at: 3022 3088 4351 3022
Size: 66 1263 5806
Fragments arranged by size:
5806 1263 66
RsrII CG'GwC_CG
Cuts at: 2169 2169
Size: 7135
SacI G_AGCT'C
Cuts at: 3003 3003
Size: 7135
SalI G'TCGA_C
Cuts at: 1936 3917 4477 1936
Size: 1981 560 4594
Fragments arranged by size:
4594 1981 560
SapI GCTCTTCn'nnn_
Cuts at: 3908 3908
Size: 7135
SbfI CC_TGCA'GG
Cuts at: 4487 4487
Size: 7135
ScaI AGT'ACT
Cuts at: 5383 7095 5383
Size: 1712 5423
SfcI C'TryA_G
Cuts at: 826 1045 2315 2338 3933 4483 5618 6299 6490
Size: 219 1270 23 1595 550 1135 681 191
Cuts at: 6490 826
Size: 1471
Fragments arranged by size:
1595 1471 1270 1135 681 550 219 191
23
SfoI GGC'GCC
Cuts at: 2144 2144
Size: 7135
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7135
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7135
SmaI CCC'GGG
Cuts at: 1872 2111 4462 1872
Size: 239 2351 4545
Fragments arranged by size:
4545 2351 239
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2383 2480 5239 6140 6402 820
Size: 197 926 440 97 2759 901 262 1553
Fragments arranged by size:
2759 1553 926 901 440 262 197 97
SnaBI TAC'GTA
Cuts at: 493 4432 4926 493
Size: 3939 494 2702
Fragments arranged by size:
3939 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7135
SphI G_CATG'C
Cuts at: 1132 4493 1132
Size: 3361 3774
SspI AAT'ATT
Cuts at: 5 52 5059 5
Size: 47 5007 2081
Fragments arranged by size:
5007 2081 47
StuI AGG'CCT
Cuts at: 1517 2622 1517
Size: 1105 6030
StyI C'CwwG_G
Cuts at: 513 1065 2022 3234 4162 4574 513
Size: 552 957 1212 928 412 3074
Fragments arranged by size:
3074 1212 957 928 552 412
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2783 4130 5162 5179 5332 5517 5962
Size: 1255 1203 1347 1032 17 153 185 445
Cuts at: 5962 325
Size: 1498
Fragments arranged by size:
1498 1347 1255 1203 1032 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2484 2544 2760
Size: 274 80 33 51 1443 507 60 216
Cuts at: 2760 2958 3360 3384 4299 5381 7093 96
Size: 198 402 24 915 1082 1712 138
Fragments arranged by size:
1712 1443 1082 915 507 402 274 216
198 138 80 60 51 33 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7095 7127 7095
Size: 32 7103
Tth111I GACn'n_nGTC
Cuts at: 1316 3245 3923 1316
Size: 1929 678 4528
Fragments arranged by size:
4528 1929 678
XbaI T'CTAG_A
Cuts at: 4471 4471
Size: 7135
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2638 4348 2638
Size: 1710 5425
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7135
XmaI C'CCGG_G
Cuts at: 1870 2109 4460 1870
Size: 239 2351 4545
Fragments arranged by size:
4545 2351 239
XmnI GAAnn'nnTTC
Cuts at: 2468 3942 5264 2468
Size: 1474 1322 4339
Fragments arranged by size:
4339 1474 1322
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3916 4943 276
Size: 53 83 186 936 2382 1027 2468
Fragments arranged by size:
2468 2382 1027 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AvrII BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I DraIII FseI FspAI NruI
NsiI PacI PspXI PsrI SacII SanDI SexAI SfiI
SrfI SwaI