Restriction map for
pFN21ASDA0989
(Circular) (Six-base) MAPSORT of: pFN21ASDA0989.seq Check: 8681 from: 1 to: 7069
pFN21ASDA0989 7069 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2902 3596 4250 2902
Size: 694 654 5721
Fragments arranged by size:
5721 694 654
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4879 278
Size: 53 83 186 936 3343 2468
Fragments arranged by size:
3343 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4412 1937
Size: 2475 4594
Acc65I G'GTAC_C
Cuts at: 2712 4390 2712
Size: 1678 5391
AclI AA'CG_TT
Cuts at: 1563 5196 5569 1563
Size: 3633 373 3063
Fragments arranged by size:
3633 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2495 2769 3944 5132 6516 6784 1632
Size: 863 274 1175 1188 1384 268 1917
Fragments arranged by size:
1917 1384 1188 1175 863 274 268
AfeI AGC'GCT
Cuts at: 3968 3968
Size: 7069
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6872
AflIII A'CryG_T
Cuts at: 1203 5968 6945 1203
Size: 4765 977 1327
Fragments arranged by size:
4765 1327 977
AgeI A'CCGG_T
Cuts at: 5900 5900
Size: 7069
AhdI GACnn_n'nnGTC
Cuts at: 5798 5798
Size: 7069
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7069
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2388 1184
Size: 1204 5865
AlwNI CAG_nnn'CTG
Cuts at: 2137 2218 2815 3406 3700 3931 4812 6384 2137
Size: 81 597 591 294 231 881 1572 2822
Fragments arranged by size:
2822 1572 881 597 591 294 231 81
ApaI G_GGCC'C
Cuts at: 2431 2545 2603 2618 2633 2431
Size: 114 58 15 15 6867
Fragments arranged by size:
6867 114 58 15 15
ApaLI G'TGCA_C
Cuts at: 3588 5126 6282 3588
Size: 1538 1156 4375
Fragments arranged by size:
4375 1538 1156
ApoI r'AATT_y
Cuts at: 1493 4378 4604 4756 6865 1493
Size: 2885 226 152 2109 1697
Fragments arranged by size:
2885 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5623 160
Size: 872 4591 1606
Fragments arranged by size:
4591 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7069
AvaI C'yCGr_G
Cuts at: 1870 1943 2044 2431 2538 2640 2938 3432 3500
Size: 73 101 387 107 102 298 494 68
Cuts at: 3500 4394 1870
Size: 894 4545
Fragments arranged by size:
4545 894 494 387 298 107 102 101
73 68
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2642 2675 2642
Size: 33 7036
BamHI G'GATC_C
Cuts at: 2757 4399 4766 2757
Size: 1642 367 5060
Fragments arranged by size:
5060 1642 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2534 2712 2773 3359 3608
Size: 325 217 627 747 178 61 586 249
Cuts at: 3608 4024 4390 5845 618
Size: 416 366 1455 1842
Fragments arranged by size:
1842 1455 747 627 586 416 366 325
249 217 178 61
BanII G_rGCy'C
Cuts at: 1391 2174 2244 2431 2545 2561 2603 2618 2633
Size: 783 70 187 114 16 42 15 15
Cuts at: 2633 2882 3035 3651 4004 4077 4388 1391
Size: 249 153 616 353 73 311 4072
Fragments arranged by size:
4072 783 616 353 311 249 187 153
114 73 70 42 16 15 15
BbeI G_GCGC'C
Cuts at: 2538 3363 2538
Size: 825 6244
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2043 3149 928
Size: 971 144 1106 4848
Fragments arranged by size:
4848 1106 971 144
BbvCI CC'TCA_GC
Cuts at: 2471 2625 2929 2975 2471
Size: 154 304 46 6565
Fragments arranged by size:
6565 304 154 46
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5260 5294 1903
Size: 34 3323 34 3678
Fragments arranged by size:
3678 3323 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4961 6171 4961
Size: 1210 5859
BclI T'GATC_A
Cuts at: 1548 3948 1548
Size: 2400 4669
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2384 2954 5680
Size: 107 122 71 631 605 712 570 2726
Cuts at: 5680 136
Size: 1525
Fragments arranged by size:
2726 1525 712 631 605 570 122 107
71
BglII A'GATC_T
Cuts at: 7064 7064
Size: 7069
BlpI GC'TnA_GC
Cuts at: 3650 3798 4486 3650
Size: 148 688 6233
Fragments arranged by size:
6233 688 148
Bme1580I G_kGCm'C
Cuts at: 1790 2431 2484 2545 2603 2618 2633 2722 2920
Size: 641 53 61 58 15 15 89 198
Cuts at: 2920 2950 3592 3613 5130 6286 1790
Size: 30 642 21 1517 1156 2573
Fragments arranged by size:
2573 1517 1156 642 641 198 89 61
58 53 30 21 15 15
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7069
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2166 4800 5758
Size: 416 53 83 399 24 749 2634 958
Cuts at: 5758 442
Size: 1753
Fragments arranged by size:
2634 1753 958 749 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2120 1056
Size: 1064 6005
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2207 2543 3047 3158 3353 3416 3560 3584
Size: 968 336 504 111 195 63 144 24
Cuts at: 3584 3627 3728 3953 3955 5729 1239
Size: 43 101 225 2 1774 2579
Fragments arranged by size:
2579 1774 968 504 336 225 195 144
111 101 63 43 24 2
Bpu10I CC'TnA_GC
Cuts at: 2471 2625 2876 2929 2975 2471
Size: 154 251 53 46 6565
Fragments arranged by size:
6565 251 154 53 46
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3560 5194 6059 6357 3560
Size: 1634 865 298 4272
Fragments arranged by size:
4272 1634 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2678 3645 3972 5732 882
Size: 617 1179 967 327 1760 2219
Fragments arranged by size:
2219 1760 1179 967 617 327
BsaAI yAC'GTr
Cuts at: 493 1204 2485 4366 4860 493
Size: 711 1281 1881 494 2702
Fragments arranged by size:
2702 1881 1281 711 494
BsaBI GATnn'nnATC
Cuts at: 3217 4358 7063 3217
Size: 1141 2705 3223
Fragments arranged by size:
3223 2705 1141
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2535 3360 4104 4876
Size: 53 83 186 936 1002 825 744 772
Cuts at: 4876 5258 5893 275
Size: 382 635 1451
Fragments arranged by size:
1451 1002 936 825 772 744 635 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 4252 5502 5900 6174 6321 6732 4252
Size: 1250 398 274 147 411 4589
Fragments arranged by size:
4589 1250 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2543 2573 3722 3752 4220
Size: 30 165 30 1140 30 1149 30 468
Cuts at: 4220 4250 1178
Size: 30 3997
Fragments arranged by size:
3997 1149 1140 468 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2032 2342 2360 2551 2917 3109 3190 3458
Size: 846 310 18 191 366 192 81 268
Cuts at: 3458 3704 4160 1186
Size: 246 456 4095
Fragments arranged by size:
4095 846 456 366 310 268 246 192
191 81 18
BseYI C'CCAG_C
Cuts at: 2315 2379 2546 3831 3842 6272 6768 2315
Size: 64 167 1285 11 2430 496 2616
Fragments arranged by size:
2616 2430 1285 496 167 64 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2042 4297 1163
Size: 573 306 2255 3935
Fragments arranged by size:
3935 2255 573 306
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4519 5280 6308 1540
Size: 275 186 2518 761 1028 2301
Fragments arranged by size:
2518 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2259 2561 2835 2882 3035 3592 3651 3918 4138
Size: 302 274 47 153 557 59 267 220
Cuts at: 4138 4208 5130 5215 6286 2259
Size: 70 922 85 1071 3042
Fragments arranged by size:
3042 1071 922 557 302 274 267 220
153 85 70 59 47
BsmI GAATG_Cn'
Cuts at: 2989 4580 4673 2989
Size: 1591 93 5385
Fragments arranged by size:
5385 1591 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2096 2096
Size: 7069
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2174 2244 2259 2431 2484 2545 2561
Size: 399 384 70 15 172 53 61 16
Cuts at: 2561 2603 2618 2633 2722 2835 2882 2920 2950
Size: 42 15 15 89 113 47 38 30
Cuts at: 2950 3035 3592 3613 3651 3918 4004 4077 4138
Size: 85 557 21 38 267 86 73 61
Cuts at: 4138 4208 4388 5130 5215 6286 1391
Size: 70 180 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 557 399 384 267 180
172 113 89 86 85 85 73 70
70 61 61 53 47 42 38 38
30 21 16 15 15 15
BspEI T'CCGG_A
Cuts at: 6732 6732
Size: 7069
BspHI T'CATG_A
Cuts at: 2680 4958 6688 2680
Size: 2278 1730 3061
Fragments arranged by size:
3061 2278 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2823 2902 3596 3894 4250 4424 844
Size: 1979 79 694 298 356 174 3489
Fragments arranged by size:
3489 1979 694 356 298 174 79
BsrBI CCG'CTC
Cuts at: 1394 1823 3010 3484 4956 1394
Size: 429 1187 474 1472 3507
Fragments arranged by size:
3507 1472 1187 474 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3418 5564 5738 65
Size: 1183 2170 2146 174 1396
Fragments arranged by size:
2170 2146 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2298 3263 5713 5900 1700
Size: 110 488 965 2450 187 2869
Fragments arranged by size:
2869 2450 965 488 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7069
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7069
BssSI C'ACGA_G
Cuts at: 5129 6141 5129
Size: 1012 6057
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7069
BstBI TT'CG_AA
Cuts at: 2007 5873 2007
Size: 3866 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7069
BstYI r'GATC_y
Cuts at: 1972 2190 2416 2757 3199 3696 3927 4399 4766
Size: 218 226 341 442 497 231 472 367
Cuts at: 4766 5151 5168 6620 6706 6718 7064 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 497 472 442 385 367 346
341 231 226 218 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2138 2138
Size: 7069
BtgI C'CryG_G
Cuts at: 513 1065 2422 2708 2722 2866 3771 3786 513
Size: 552 1357 286 14 144 905 15 3796
Fragments arranged by size:
3796 1357 905 552 286 144 15 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6330
Fragments arranged by size:
6330 683 56
BtsI GCAGTG_nn'
Cuts at: 2691 2950 3347 3631 3637 3951 4585 5379 5399
Size: 259 397 284 6 314 634 794 20
Cuts at: 5399 2691
Size: 4361
Fragments arranged by size:
4361 794 634 397 314 284 259 20
6
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7069
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7034
DraI TTT'AAA
Cuts at: 4373 4373
Size: 7069
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6076 809
Size: 507 4760 1802
Fragments arranged by size:
4760 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2396 2863 3049
Size: 54 1425 50 275 18 566 467 186
Cuts at: 3049 3282 3877 4516 5405 7034 8
Size: 233 595 639 889 1629 43
Fragments arranged by size:
1629 1425 889 639 595 566 467 275
233 186 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4516 1537
Size: 275 2704 4090
Fragments arranged by size:
4090 2704 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3435 4084 4999 1353
Size: 2082 649 915 3423
Fragments arranged by size:
3423 2082 915 649
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5656 6030 6176 387
Size: 1064 138 4067 374 146 1280
Fragments arranged by size:
4067 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2559 2880 3033 3649 2559
Size: 321 153 616 5979
Fragments arranged by size:
5979 616 321 153
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2207 2495 2543 2769 3047 3158 3353
Size: 393 575 288 48 226 278 111 195
Cuts at: 3353 3416 3560 3584 3627 3728 3944 3953 3955
Size: 63 144 24 43 101 216 9 2
Cuts at: 3955 5132 5729 6516 6784 1239
Size: 1177 597 787 268 1524
Fragments arranged by size:
1524 1177 787 597 575 393 288 278
268 226 216 195 144 111 101 63
48 43 24 9 2
EcoNI CCTnn'n_nnAGG
Cuts at: 2069 3566 3935 2069
Size: 1497 369 5203
Fragments arranged by size:
5203 1497 369
EcoO109I rG'GnC_Cy
Cuts at: 2134 2391 2427 2428 2458 2542 2599 2600 2614
Size: 257 36 1 30 84 57 1 14
Cuts at: 2614 2615 2629 2645 3133 3580 3955 2134
Size: 1 14 16 488 447 375 5248
Fragments arranged by size:
5248 488 447 375 257 84 57 36
30 16 14 14 1 1 1
EcoRI G'AATT_C
Cuts at: 4378 4756 4378
Size: 378 6691
FspI TGC'GCA
Cuts at: 3417 3452 5575 3417
Size: 35 2123 4911
Fragments arranged by size:
4911 2123 35
HaeII r_GCGC'y
Cuts at: 1678 2538 3363 3500 3657 3707 3970 6216 1678
Size: 860 825 137 157 50 263 2246 2531
Fragments arranged by size:
2531 2246 860 825 263 157 137 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4220 4252 5710 5742 5784
Size: 32 135 32 2648 32 1458 32 42
Cuts at: 5784 5816 1373
Size: 32 2626
Fragments arranged by size:
2648 2626 1458 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4413 4659 669
Size: 988 281 2475 246 3079
Fragments arranged by size:
3079 2475 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7069
HpaI GTT'AAC
Cuts at: 4659 4659
Size: 7069
KasI G'GCGC_C
Cuts at: 2534 3359 2534
Size: 825 6244
KpnI G_GTAC'C
Cuts at: 2716 4394 2716
Size: 1678 5391
MfeI C'AATT_G
Cuts at: 4668 4668
Size: 7069
MluI A'CGCG_T
Cuts at: 6945 6945
Size: 7069
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2423 2815 3844 6183 6367 1305
Size: 1118 392 1029 2339 184 2007
Fragments arranged by size:
2339 2007 1118 1029 392 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3284 3879 10
Size: 54 1425 343 1452 595 3200
Fragments arranged by size:
3200 1452 1425 595 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5027 5386 5545 518
Size: 648 3861 359 159 2042
Fragments arranged by size:
3861 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2080 2581 2724 2855 3082 3376 3508 3538
Size: 474 501 143 131 227 294 132 30
Cuts at: 3538 4485 5162 6310 6555 1606
Size: 947 677 1148 245 2120
Fragments arranged by size:
2120 1148 947 677 501 474 294 245
227 143 132 131 30
NaeI GCC'GGC
Cuts at: 3265 3265
Size: 7069
NarI GG'CG_CC
Cuts at: 2535 3360 2535
Size: 825 6244
NcoI C'CATG_G
Cuts at: 513 1065 2866 3771 513
Size: 552 1801 905 3811
Fragments arranged by size:
3811 1801 905 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7069
NgoMIV G'CCGG_C
Cuts at: 3263 3263
Size: 7069
NheI G'CTAG_C
Cuts at: 1052 2116 1052
Size: 1064 6005
NotI GC'GGCC_GC
Cuts at: 4516 4516
Size: 7069
NspI r_CATG'y
Cuts at: 1132 1229 2405 2747 4427 5972 1132
Size: 97 1176 342 1680 1545 2229
Fragments arranged by size:
2229 1680 1545 1176 342 97
PasI CC'CwG_GG
Cuts at: 1348 2245 4060 1348
Size: 897 1815 4357
Fragments arranged by size:
4357 1815 897
PciI A'CATG_T
Cuts at: 5968 5968
Size: 7069
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3931 1108
Size: 2823 4246
PfoI T'CCnGG_A
Cuts at: 2070 3930 3971 4329 2070
Size: 1860 41 358 4810
Fragments arranged by size:
4810 1860 358 41
PmeI GTTT'AAAC
Cuts at: 4373 4373
Size: 7069
PmlI CAC'GTG
Cuts at: 2485 2485
Size: 7069
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5138 5170 6651 6683 5138
Size: 32 1481 32 5524
Fragments arranged by size:
5524 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2391 2645 3955 2391
Size: 254 1310 5505
Fragments arranged by size:
5505 1310 254
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7069
PsiI TTA'TAA
Cuts at: 4639 4639
Size: 7069
PspOMI G'GGCC_C
Cuts at: 2427 2541 2599 2614 2629 2427
Size: 114 58 15 15 6867
Fragments arranged by size:
6867 114 58 15 15
PspXI vC'TCGA_Gb
Cuts at: 3432 3432
Size: 7069
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2146 2178 2279 2311 2146
Size: 32 101 32 6904
Fragments arranged by size:
6904 101 32 32
PstI C_TGCA'G
Cuts at: 830 2223 2766 2820 2997 3018 3492 3513 4421
Size: 1393 543 54 177 21 474 21 908
Cuts at: 4421 830
Size: 3478
Fragments arranged by size:
3478 1393 908 543 474 177 54 21
21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7069
PvuII CAG'CTG
Cuts at: 2080 2581 2855 3508 3538 2080
Size: 501 274 653 30 5611
Fragments arranged by size:
5611 653 501 274 30
SacI G_AGCT'C
Cuts at: 2561 2882 3035 3651 2561
Size: 321 153 616 5979
Fragments arranged by size:
5979 616 321 153
SacII CC_GC'GG
Cuts at: 2725 2725
Size: 7069
SalI G'TCGA_C
Cuts at: 1936 4411 1936
Size: 2475 4594
SanDI GG'GwC_CC
Cuts at: 2391 2391
Size: 7069
SbfI CC_TGCA'GG
Cuts at: 2766 4421 2766
Size: 1655 5414
ScaI AGT'ACT
Cuts at: 2369 5317 7029 2369
Size: 2948 1712 2409
Fragments arranged by size:
2948 2409 1712
SfcI C'TryA_G
Cuts at: 826 1045 2219 2762 2816 2993 3014 3488 3509
Size: 219 1174 543 54 177 21 474 21
Cuts at: 3509 4122 4311 4417 5552 6233 6424 826
Size: 613 189 106 1135 681 191 1471
Fragments arranged by size:
1471 1174 1135 681 613 543 474 219
191 189 177 106 54 21 21
SfoI GGC'GCC
Cuts at: 2536 3361 2536
Size: 825 6244
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7069
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7069
SmaI CCC'GGG
Cuts at: 1872 2433 2540 4396 1872
Size: 561 107 1856 4545
Fragments arranged by size:
4545 1856 561 107
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3432 3575 5173 6074 6336 820
Size: 197 926 1489 143 1598 901 262 1553
Fragments arranged by size:
1598 1553 1489 926 901 262 197 143
SnaBI TAC'GTA
Cuts at: 493 4366 4860 493
Size: 3873 494 2702
Fragments arranged by size:
3873 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7069
SphI G_CATG'C
Cuts at: 1132 2747 4427 1132
Size: 1615 1680 3774
Fragments arranged by size:
3774 1680 1615
SrfI GCCC'GGGC
Cuts at: 2540 2540
Size: 7069
SspI AAT'ATT
Cuts at: 5 52 4993 5
Size: 47 4941 2081
Fragments arranged by size:
4941 2081 47
StuI AGG'CCT
Cuts at: 1517 2180 2924 3464 1517
Size: 663 744 540 5122
Fragments arranged by size:
5122 744 663 540
StyI C'CwwG_G
Cuts at: 513 1065 2866 3771 4508 513
Size: 552 1801 905 737 3074
Fragments arranged by size:
3074 1801 905 737 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2427 3856 4153 4223 5096 5113 5266
Size: 1255 847 1429 297 70 873 17 153
Cuts at: 5266 5451 5896 325
Size: 185 445 1498
Fragments arranged by size:
1498 1429 1255 873 847 445 297 185
153 70 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2367 2671 5315
Size: 274 80 33 51 1443 390 304 2644
Cuts at: 5315 7027 96
Size: 1712 138
Fragments arranged by size:
2644 1712 1443 390 304 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2543 2575 7029 7061 2543
Size: 32 4454 32 2551
Fragments arranged by size:
4454 2551 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7069
XbaI T'CTAG_A
Cuts at: 4405 4405
Size: 7069
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2029 2029
Size: 7069
XhoI C'TCGA_G
Cuts at: 1943 3432 1943
Size: 1489 5580
XmaI C'CCGG_G
Cuts at: 1870 2431 2538 4394 1870
Size: 561 107 1856 4545
Fragments arranged by size:
4545 1856 561 107
XmnI GAAnn'nnTTC
Cuts at: 5198 5198
Size: 7069
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4877 276
Size: 53 83 186 936 3343 2468
Fragments arranged by size:
3343 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII SacI SacII SalI SanDI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BfrBI BplI BsiWI BstXI BstZ17I
DraIII EcoRV FalI FseI FspAI NruI NsiI PacI
RsrII SapI SexAI SfiI SwaI