Restriction map for
pFN21ASDA0988
(Circular) (Six-base) MAPSORT of: pFN21ASDA0988.seq Check: 111 from: 1 to: 8305
pFN21ASDA0988 8305 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3084 5448 6115 278
Size: 53 83 186 936 1548 2364 667 2468
Fragments arranged by size:
2468 2364 1548 936 667 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5009 5648 1937
Size: 3072 639 4594
Fragments arranged by size:
4594 3072 639
Acc65I G'GTAC_C
Cuts at: 2973 5626 2973
Size: 2653 5652
AclI AA'CG_TT
Cuts at: 1563 6432 6805 1563
Size: 4869 373 3063
Fragments arranged by size:
4869 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2969 3149 3226 3377 3491 4007 5071 5174
Size: 1337 180 77 151 114 516 1064 103
Cuts at: 5174 6368 7752 8020 1632
Size: 1194 1384 268 1917
Fragments arranged by size:
1917 1384 1337 1194 1064 516 268 180
151 114 103 77
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8108
AflIII A'CryG_T
Cuts at: 1203 2669 3429 7204 8181 1203
Size: 1466 760 3775 977 1327
Fragments arranged by size:
3775 1466 1327 977 760
AgeI A'CCGG_T
Cuts at: 7136 7136
Size: 8305
AhdI GACnn_n'nnGTC
Cuts at: 3855 7034 3855
Size: 3179 5126
AleI CACnn'nnGTG
Cuts at: 1166 3146 1166
Size: 1980 6325
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5323 1184
Size: 4139 4166
AlwNI CAG_nnn'CTG
Cuts at: 2645 2791 2896 2932 3118 3469 3554 3744 3985
Size: 146 105 36 186 351 85 190 241
Cuts at: 3985 4303 5077 6048 7620 2645
Size: 318 774 971 1572 3330
Fragments arranged by size:
3330 1572 971 774 351 318 241 190
186 146 105 85 36
ApaLI G'TGCA_C
Cuts at: 2159 3498 4902 6362 7518 2159
Size: 1339 1404 1460 1156 2946
Fragments arranged by size:
2946 1460 1404 1339 1156
ApoI r'AATT_y
Cuts at: 1493 2333 5263 5614 5840 5992 8101 1493
Size: 840 2930 351 226 152 2109 1697
Fragments arranged by size:
2930 2109 1697 840 351 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6859 160
Size: 872 5827 1606
Fragments arranged by size:
5827 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8305
AvaI C'yCGr_G
Cuts at: 1870 1943 2057 2130 2631 3493 3806 3832 4322
Size: 73 114 73 501 862 313 26 490
Cuts at: 4322 4439 5630 1870
Size: 117 1191 4545
Fragments arranged by size:
4545 1191 862 501 490 313 117 114
73 73 26
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2965 2998 2965
Size: 33 8272
BamHI G'GATC_C
Cuts at: 5635 6002 5635
Size: 367 7938
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2856 2973 3386 3401 3802
Size: 325 217 627 1069 117 413 15 401
Cuts at: 3802 4361 4625 5578 5591 5626 7081 618
Size: 559 264 953 13 35 1455 1842
Fragments arranged by size:
1842 1455 1069 953 627 559 413 401
325 264 217 117 35 15 13
BanII G_rGCy'C
Cuts at: 1391 3205 4528 5624 1391
Size: 1814 1323 1096 4072
Fragments arranged by size:
4072 1814 1323 1096
BbeI G_GCGC'C
Cuts at: 4365 4365
Size: 8305
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2829 3820 5162 5405 928
Size: 971 930 991 1342 243 3828
Fragments arranged by size:
3828 1342 991 971 930 243
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3763 3797 4194 4228 6496 6530 1903
Size: 34 1826 34 397 34 2268 34 3678
Fragments arranged by size:
3678 2268 1826 397 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6197 7407 6197
Size: 1210 7095
BclI T'GATC_A
Cuts at: 1548 4290 1548
Size: 2742 5563
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2865 3800 4748
Size: 107 122 71 631 605 1193 935 948
Cuts at: 4748 6916 136
Size: 2168 1525
Fragments arranged by size:
2168 1525 1193 948 935 631 605 122
107 71
BglII A'GATC_T
Cuts at: 3862 5106 8300 3862
Size: 1244 3194 3867
Fragments arranged by size:
3867 3194 1244
BlpI GC'TnA_GC
Cuts at: 4308 5722 4308
Size: 1414 6891
Bme1580I G_kGCm'C
Cuts at: 1790 2163 3404 3502 3590 4628 4842 4906 5583
Size: 373 1241 98 88 1038 214 64 677
Cuts at: 5583 6366 7522 1790
Size: 783 1156 2573
Fragments arranged by size:
2573 1241 1156 1038 783 677 373 214
98 88 64
BmgBI CAC'GTC
Cuts at: 1318 2672 4621 4966 5335 1318
Size: 1354 1949 345 369 4288
Fragments arranged by size:
4288 1949 1354 369 345
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2942 3754 6036
Size: 416 53 83 399 24 1525 812 2282
Cuts at: 6036 6994 442
Size: 958 1753
Fragments arranged by size:
2282 1753 1525 958 812 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8305
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4061 4061
Size: 8305
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2213 2289 2734 4379 4918 6965 1239
Size: 974 76 445 1645 539 2047 2579
Fragments arranged by size:
2579 2047 1645 974 539 445 76
Bpu10I CC'TnA_GC
Cuts at: 2030 4695 2030
Size: 2665 5640
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4159 6430 7295 7593 4159
Size: 2271 865 298 4871
Fragments arranged by size:
4871 2271 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2120 6968 882
Size: 617 621 4848 2219
Fragments arranged by size:
4848 2219 621 617
BsaAI yAC'GTr
Cuts at: 493 1204 3430 4837 5170 5602 6096 493
Size: 711 2226 1407 333 432 494 2702
Fragments arranged by size:
2702 2226 1407 711 494 432 333
BsaBI GATnn'nnATC
Cuts at: 8299 8299
Size: 8305
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3081 3417 4362 5445
Size: 53 83 186 936 1548 336 945 1083
Cuts at: 5445 6112 6494 7129 275
Size: 667 382 635 1451
Fragments arranged by size:
1548 1451 1083 945 936 667 635 382
336 186 83 53
BsaWI w'CCGG_w
Cuts at: 5394 6738 7136 7410 7557 7968 5394
Size: 1344 398 274 147 411 5731
Fragments arranged by size:
5731 1344 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4539 4569 1178
Size: 30 165 30 3136 30 4914
Fragments arranged by size:
4914 3136 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2075 2078 2534 3005 4298 4340 4863 5222
Size: 889 3 456 471 1293 42 523 359
Cuts at: 5222 5353 5360 1186
Size: 131 7 4131
Fragments arranged by size:
4131 1293 889 523 471 456 359 131
42 7 3
BseYI C'CCAG_C
Cuts at: 2140 2550 2908 3298 3795 4579 4743 4932 4972
Size: 410 358 390 497 784 164 189 40
Cuts at: 4972 5571 5583 7508 8004 2140
Size: 599 12 1925 496 2441
Fragments arranged by size:
2441 1925 784 599 497 496 410 390
358 189 164 40 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2945 3767 3998 4793 5339 1163
Size: 573 1209 822 231 795 546 4129
Fragments arranged by size:
4129 1209 822 795 573 546 231
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2046 3147 5755 6516 7544 1540
Size: 275 186 45 1101 2608 761 1028 2301
Fragments arranged by size:
2608 2301 1101 1028 761 275 186 45
BsiHKAI G_wGCw'C
Cuts at: 2163 3502 4780 4906 5175 5487 6366 6451 7522
Size: 1339 1278 126 269 312 879 85 1071
Cuts at: 7522 2163
Size: 2946
Fragments arranged by size:
2946 1339 1278 1071 879 312 269 126
85
BsmI GAATG_Cn'
Cuts at: 5055 5816 5909 5055
Size: 761 93 7451
Fragments arranged by size:
7451 761 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3330 4464 3330
Size: 1134 7171
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2163 3205 3404 3502 3590 4528 4628
Size: 399 373 1042 199 98 88 938 100
Cuts at: 4628 4780 4842 4906 5175 5487 5583 5624 6366
Size: 152 62 64 269 312 96 41 742
Cuts at: 6366 6451 7522 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 1042 938 742 399 373 312
269 199 152 100 98 96 88 85
64 62 41
BspEI T'CCGG_A
Cuts at: 5394 7968 5394
Size: 2574 5731
BspHI T'CATG_A
Cuts at: 2392 4047 6194 7924 2392
Size: 1655 2147 1730 2773
Fragments arranged by size:
2773 2147 1730 1655
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2510 4390 5660 844
Size: 1666 1880 1270 3489
Fragments arranged by size:
3489 1880 1666 1270
BsrBI CCG'CTC
Cuts at: 1394 1823 2197 6192 1394
Size: 429 374 3995 3507
Fragments arranged by size:
3995 3507 429 374
BsrDI GCAATG_nn'
Cuts at: 65 1248 4139 4485 5426 6800 6974 65
Size: 1183 2891 346 941 1374 174 1396
Fragments arranged by size:
2891 1396 1374 1183 941 346 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2041 3683 4389 6949 7136 1700
Size: 110 231 1642 706 2560 187 2869
Fragments arranged by size:
2869 2560 1642 706 231 187 110
BsrGI T'GTAC_A
Cuts at: 96 2317 3918 96
Size: 2221 1601 4483
Fragments arranged by size:
4483 2221 1601
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8305
BssSI C'ACGA_G
Cuts at: 3332 6365 7377 3332
Size: 3033 1012 4260
Fragments arranged by size:
4260 3033 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3554 3905 3976 4731 794
Size: 2760 351 71 755 4368
Fragments arranged by size:
4368 2760 755 351 71
BstBI TT'CG_AA
Cuts at: 2007 7109 2007
Size: 5102 3203
BstEII G'GTnAC_C
Cuts at: 1184 3724 1184
Size: 2540 5765
BstXI CCAn_nnnn'nTGG
Cuts at: 3205 3749 4508 4660 4940 3205
Size: 544 759 152 280 6570
Fragments arranged by size:
6570 759 544 280 152
BstYI r'GATC_y
Cuts at: 1972 3647 3862 4267 4663 5106 5635 6002 6387
Size: 1675 215 405 396 443 529 367 385
Cuts at: 6387 6404 7856 7942 7954 8300 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1675 1452 529 443 405 396 385
367 346 215 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2852 3462 2852
Size: 610 7695
BtgI C'CryG_G
Cuts at: 513 1065 2022 2046 2769 4602 5179 5202 5403
Size: 552 957 24 723 1833 577 23 201
Cuts at: 5403 513
Size: 3415
Fragments arranged by size:
3415 1833 957 723 577 552 201 24
23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5281 487
Size: 683 56 4055 3511
Fragments arranged by size:
4055 3511 683 56
BtsI GCAGTG_nn'
Cuts at: 3981 4065 4240 5307 5493 5523 5821 6615 6635
Size: 84 175 1067 186 30 298 794 20
Cuts at: 6635 3981
Size: 5651
Fragments arranged by size:
5651 1067 794 298 186 175 84 30
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8305
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8270
DraI TTT'AAA
Cuts at: 2332 2806 5609 2332
Size: 474 2803 5028
Fragments arranged by size:
5028 2803 474
DraIII CAC_nnn'GTG
Cuts at: 5499 5499
Size: 8305
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7312 809
Size: 507 5996 1802
Fragments arranged by size:
5996 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 5406 5752
Size: 54 1425 50 275 18 213 3363 346
Cuts at: 5752 6641 8270 8
Size: 889 1629 43
Fragments arranged by size:
3363 1629 1425 889 346 275 213 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2043 5752 1537
Size: 275 231 3709 4090
Fragments arranged by size:
4090 3709 275 231
EarI CTCTTCn'nnn_
Cuts at: 1353 3538 6235 1353
Size: 2185 2697 3423
Fragments arranged by size:
3423 2697 2185
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2084 3224 6892 7266 7412 387
Size: 1064 138 495 1140 3668 374 146 1280
Fragments arranged by size:
3668 1280 1140 1064 495 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2213 2289 2734 2969 3149 3226 3377
Size: 393 581 76 445 235 180 77 151
Cuts at: 3377 3491 4007 4379 4918 5071 5174 6368 6965
Size: 114 516 372 539 153 103 1194 597
Cuts at: 6965 7752 8020 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1194 787 597 581 539 516 445
393 372 268 235 180 153 151 114
103 77 76
EcoNI CCTnn'n_nnAGG
Cuts at: 2153 3020 2153
Size: 867 7438
EcoO109I rG'GnC_Cy
Cuts at: 2788 3982 5074 5094 5347 5568 2788
Size: 1194 1092 20 253 221 5525
Fragments arranged by size:
5525 1194 1092 253 221 20
EcoRI G'AATT_C
Cuts at: 5263 5614 5992 5263
Size: 351 378 7576
Fragments arranged by size:
7576 378 351
EcoRV GAT'ATC
Cuts at: 5275 5275
Size: 8305
FspI TGC'GCA
Cuts at: 3898 6811 3898
Size: 2913 5392
HaeII r_GCGC'y
Cuts at: 1678 2102 2138 3290 3365 3782 3819 4365 7452
Size: 424 36 1152 75 417 37 546 3087
Cuts at: 7452 1678
Size: 2531
Fragments arranged by size:
3087 2531 1152 546 424 417 75 37
36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2285 2317 3841 3847 3873
Size: 32 135 32 713 32 1524 6 26
Cuts at: 3873 3879 4871 4903 5123 5155 6946 6978 7020
Size: 6 992 32 220 32 1791 32 42
Cuts at: 7020 7052 1373
Size: 32 2626
Fragments arranged by size:
2626 1791 1524 992 713 220 135 42
32 32 32 32 32 32 32 26
6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 5010 5649 5895 669
Size: 988 281 3072 639 246 3079
Fragments arranged by size:
3079 3072 988 639 281 246
HindIII A'AGCT_T
Cuts at: 748 2457 2918 748
Size: 1709 461 6135
Fragments arranged by size:
6135 1709 461
HpaI GTT'AAC
Cuts at: 5895 5895
Size: 8305
KasI G'GCGC_C
Cuts at: 4361 4361
Size: 8305
KpnI G_GTAC'C
Cuts at: 2977 5630 2977
Size: 2653 5652
MfeI C'AATT_G
Cuts at: 5904 5904
Size: 8305
MluI A'CGCG_T
Cuts at: 8181 8181
Size: 8305
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2223 2259 3675 7419 7603 1305
Size: 918 36 1416 3744 184 2007
Fragments arranged by size:
3744 2007 1416 918 184 36
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6483
Fragments arranged by size:
6483 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2768 3146 3747 4716 5300 6263 6622
Size: 648 1602 378 601 969 584 963 359
Cuts at: 6622 6781 518
Size: 159 2042
Fragments arranged by size:
2042 1602 969 963 648 601 584 378
359 159
MspA1I CmG'CkG
Cuts at: 1606 2048 2642 3115 3623 5091 5149 5359 5389
Size: 442 594 473 508 1468 58 210 30
Cuts at: 5389 5575 5721 6398 7546 7791 1606
Size: 186 146 677 1148 245 2120
Fragments arranged by size:
2120 1468 1148 677 594 508 473 442
245 210 186 146 58 30
NaeI GCC'GGC
Cuts at: 3685 4391 3685
Size: 706 7599
NarI GG'CG_CC
Cuts at: 4362 4362
Size: 8305
NcoI C'CATG_G
Cuts at: 513 1065 2022 2769 513
Size: 552 957 747 6049
Fragments arranged by size:
6049 957 747 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8305
NgoMIV G'CCGG_C
Cuts at: 3683 4389 3683
Size: 706 7599
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8305
NotI GC'GGCC_GC
Cuts at: 5752 5752
Size: 8305
NspI r_CATG'y
Cuts at: 1132 1229 2479 5663 7208 1132
Size: 97 1250 3184 1545 2229
Fragments arranged by size:
3184 2229 1545 1250 97
PasI CC'CwG_GG
Cuts at: 1348 4990 5077 1348
Size: 3642 87 4576
Fragments arranged by size:
4576 3642 87
PciI A'CATG_T
Cuts at: 7204 7204
Size: 8305
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8305
PfoI T'CCnGG_A
Cuts at: 2706 3249 3564 4463 2706
Size: 543 315 899 6548
Fragments arranged by size:
6548 899 543 315
PmeI GTTT'AAAC
Cuts at: 5609 5609
Size: 8305
PmlI CAC'GTG
Cuts at: 4837 5170 4837
Size: 333 7972
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6374 6406 7887 7919 6374
Size: 32 1481 32 6760
Fragments arranged by size:
6760 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 5094 5094
Size: 8305
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8305
PsiI TTA'TAA
Cuts at: 3668 5875 3668
Size: 2207 6098
PstI C_TGCA'G
Cuts at: 830 2787 2873 3012 3163 3395 3542 5106 5358
Size: 1957 86 139 151 232 147 1564 252
Cuts at: 5358 5657 830
Size: 299 3478
Fragments arranged by size:
3478 1957 1564 299 252 232 151 147
139 86
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8305
PvuII CAG'CTG
Cuts at: 2642 3115 3623 5359 5575 2642
Size: 473 508 1736 216 5372
Fragments arranged by size:
5372 1736 508 473 216
SacII CC_GC'GG
Cuts at: 2049 2049
Size: 8305
SalI G'TCGA_C
Cuts at: 1936 5008 5647 1936
Size: 3072 639 4594
Fragments arranged by size:
4594 3072 639
SanDI GG'GwC_CC
Cuts at: 5094 5094
Size: 8305
SapI GCTCTTCn'nnn_
Cuts at: 3538 3538
Size: 8305
SbfI CC_TGCA'GG
Cuts at: 5657 5657
Size: 8305
ScaI AGT'ACT
Cuts at: 6553 8265 6553
Size: 1712 6593
SexAI A'CCwGG_T
Cuts at: 3720 3720
Size: 8305
SfcI C'TryA_G
Cuts at: 826 1045 2783 2869 3008 3159 3391 3538 5102
Size: 219 1738 86 139 151 232 147 1564
Cuts at: 5102 5354 5437 5653 6788 7469 7660 826
Size: 252 83 216 1135 681 191 1471
Fragments arranged by size:
1738 1564 1471 1135 681 252 232 219
216 191 151 147 139 86 83
SfiI GGCCn_nnn'nGGCC
Cuts at: 4748 4748
Size: 8305
SfoI GGC'GCC
Cuts at: 4363 4363
Size: 8305
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8305
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8305
SmaI CCC'GGG
Cuts at: 1872 2132 5632 1872
Size: 260 3500 4545
Fragments arranged by size:
4545 3500 260
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3493 4174 6409 7310 7572 820
Size: 197 926 1550 681 2235 901 262 1553
Fragments arranged by size:
2235 1553 1550 926 901 681 262 197
SnaBI TAC'GTA
Cuts at: 493 5602 6096 493
Size: 5109 494 2702
Fragments arranged by size:
5109 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8305
SphI G_CATG'C
Cuts at: 1132 5663 1132
Size: 4531 3774
SspI AAT'ATT
Cuts at: 5 52 2802 4241 6229 5
Size: 47 2750 1439 1988 2081
Fragments arranged by size:
2750 2081 1988 1439 47
StuI AGG'CCT
Cuts at: 1517 3312 4916 1517
Size: 1795 1604 4906
Fragments arranged by size:
4906 1795 1604
StyI C'CwwG_G
Cuts at: 513 1065 2022 2352 2445 2769 3165 4356 4939
Size: 552 957 330 93 324 396 1191 583
Cuts at: 4939 5744 513
Size: 805 3074
Fragments arranged by size:
3074 1191 957 805 583 552 396 330
324 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2564 3071 3540 4946 5042 5192 6332
Size: 1255 984 507 469 1406 96 150 1140
Cuts at: 6332 6349 6502 6687 7132 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1406 1255 1140 984 507 469 445
185 153 150 96 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2317 3696 3918
Size: 274 80 33 51 1443 340 1379 222
Cuts at: 3918 5043 6551 8263 96
Size: 1125 1508 1712 138
Fragments arranged by size:
1712 1508 1443 1379 1125 340 274 222
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8265 8297 8265
Size: 32 8273
Tth111I GACn'n_nGTC
Cuts at: 1316 3080 3143 5005 1316
Size: 1764 63 1862 4616
Fragments arranged by size:
4616 1862 1764 63
XbaI T'CTAG_A
Cuts at: 5641 5641
Size: 8305
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3658 4879 5572 3658
Size: 1221 693 6391
Fragments arranged by size:
6391 1221 693
XhoI C'TCGA_G
Cuts at: 1943 3493 1943
Size: 1550 6755
XmaI C'CCGG_G
Cuts at: 1870 2130 5630 1870
Size: 260 3500 4545
Fragments arranged by size:
4545 3500 260
XmnI GAAnn'nnTTC
Cuts at: 6434 6434
Size: 8305
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3082 5446 6113 276
Size: 53 83 186 936 1548 2364 667 2468
Fragments arranged by size:
2468 2364 1548 936 667 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbvCI BfrBI
BsiWI BstZ17I EcoICRI FalI FseI FspAI NruI NsiI
PacI PspOMI PspXI PsrI RsrII SacI SrfI SwaI