Restriction map for
pFN21ASDA0979
(Circular) (Six-base) MAPSORT of: pFN21ASDA0979.seq Check: 3347 from: 1 to: 9070
pFN21ASDA0979 9070 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6880 278
Size: 53 83 186 936 5344 2468
Fragments arranged by size:
5344 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2556 6413 1937
Size: 619 3857 4594
Fragments arranged by size:
4594 3857 619
Acc65I G'GTAC_C
Cuts at: 2632 6391 2632
Size: 3759 5311
AclI AA'CG_TT
Cuts at: 1563 7197 7570 1563
Size: 5634 373 3063
Fragments arranged by size:
5634 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2190 2199 2322 2708 3771 4961 5775 7133
Size: 558 9 123 386 1063 1190 814 1358
Cuts at: 7133 8517 8785 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1358 1190 1063 814 558 386
268 123 9
AflII C'TTAA_G
Cuts at: 820 1017 3980 4573 820
Size: 197 2963 593 5317
Fragments arranged by size:
5317 2963 593 197
AflIII A'CryG_T
Cuts at: 1203 7969 8946 1203
Size: 6766 977 1327
Fragments arranged by size:
6766 1327 977
AgeI A'CCGG_T
Cuts at: 7901 7901
Size: 9070
AhdI GACnn_n'nnGTC
Cuts at: 7799 7799
Size: 9070
AleI CACnn'nnGTG
Cuts at: 1166 3674 1166
Size: 2508 6562
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9070
AlwNI CAG_nnn'CTG
Cuts at: 2743 3296 4082 5798 6813 8385 2743
Size: 553 786 1716 1015 1572 3428
Fragments arranged by size:
3428 1716 1572 1015 786 553
ApaLI G'TGCA_C
Cuts at: 3854 7127 8283 3854
Size: 3273 1156 4641
Fragments arranged by size:
4641 3273 1156
ApoI r'AATT_y
Cuts at: 1493 2478 2647 2855 3026 3264 3625 3665 4092
Size: 985 169 208 171 238 361 40 427
Cuts at: 4092 4497 5332 6379 6605 6757 8866 1493
Size: 405 835 1047 226 152 2109 1697
Fragments arranged by size:
2109 1697 1047 985 835 427 405 361
238 226 208 171 169 152 40
AseI AT'TA_AT
Cuts at: 160 1032 5105 7624 160
Size: 872 4073 2519 1606
Fragments arranged by size:
4073 2519 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9070
AvaI C'yCGr_G
Cuts at: 1870 1943 6395 1870
Size: 73 4452 4545
Fragments arranged by size:
4545 4452 73
BamHI G'GATC_C
Cuts at: 6400 6767 6400
Size: 367 8703
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2632 6391 7846 618
Size: 325 217 627 845 3759 1455 1842
Fragments arranged by size:
3759 1842 1455 845 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2390 2570 2808 4891 6389 1391
Size: 999 180 238 2083 1498 4072
Fragments arranged by size:
4072 2083 1498 999 238 180
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5583 6157 928
Size: 971 3684 574 3841
Fragments arranged by size:
3841 3684 971 574
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7261 7295 1903
Size: 34 5324 34 3678
Fragments arranged by size:
5324 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2371 2605 4229 6962 8172 2371
Size: 234 1624 2733 1210 3269
Fragments arranged by size:
3269 2733 1624 1210 234
BclI T'GATC_A
Cuts at: 1548 3181 3822 3949 4357 1548
Size: 1633 641 127 408 6261
Fragments arranged by size:
6261 1633 641 408 127
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2965 7681 136
Size: 107 122 71 631 605 1293 4716 1525
Fragments arranged by size:
4716 1525 1293 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4408 9065 4408
Size: 4657 4413
BlpI GC'TnA_GC
Cuts at: 6487 6487
Size: 9070
Bme1580I G_kGCm'C
Cuts at: 1790 3858 4855 7131 8287 1790
Size: 2068 997 2276 1156 2573
Fragments arranged by size:
2573 2276 2068 1156 997
BmgBI CAC'GTC
Cuts at: 1318 4652 1318
Size: 3334 5736
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4243 6801 7759
Size: 416 53 83 399 24 2826 2558 958
Cuts at: 7759 442
Size: 1753
Fragments arranged by size:
2826 2558 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3036 1056
Size: 1980 7090
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2715 4905 2715
Size: 2190 6880
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5970 7730 1239
Size: 4731 1760 2579
Fragments arranged by size:
4731 2579 1760
Bpu10I CC'TnA_GC
Cuts at: 3648 3648
Size: 9070
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2213 2438 2685 4853 7195 8060 8358 2213
Size: 225 247 2168 2342 865 298 2925
Fragments arranged by size:
2925 2342 2168 865 298 247 225
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4238 7733 882
Size: 617 2739 3495 2219
Fragments arranged by size:
3495 2739 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 6367 6861 493
Size: 711 5163 494 2702
Fragments arranged by size:
5163 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 9064 9064
Size: 9070
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6877 7259 7894 275
Size: 53 83 186 936 5344 382 635 1451
Fragments arranged by size:
5344 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 7503 7901 8175 8322 8733 7503
Size: 398 274 147 411 7840
Fragments arranged by size:
7840 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 8845
Fragments arranged by size:
8845 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2123 2897 5714 6011 6014 6320 6323 1186
Size: 937 774 2817 297 3 306 3 3933
Fragments arranged by size:
3933 2817 937 774 306 297 3 3
BseYI C'CCAG_C
Cuts at: 2487 2847 4667 8273 8769 2487
Size: 360 1820 3606 496 2788
Fragments arranged by size:
3606 2788 1820 496 360
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4068 1163
Size: 573 2332 6165
Fragments arranged by size:
6165 2332 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6520 7281 8309 1540
Size: 275 186 4519 761 1028 2301
Fragments arranged by size:
4519 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2570 2808 3858 3883 4891 6079 7131 7216 8287
Size: 238 1050 25 1008 1188 1052 85 1071
Cuts at: 8287 2570
Size: 3353
Fragments arranged by size:
3353 1188 1071 1052 1050 1008 238 85
25
BsmI GAATG_Cn'
Cuts at: 4769 6581 6674 4769
Size: 1812 93 7165
Fragments arranged by size:
7165 1812 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2113 5619 2113
Size: 3506 5564
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2390 2570 2808 3858 3883 4855 4891
Size: 399 600 180 238 1050 25 972 36
Cuts at: 4891 6079 6389 7131 7216 8287 1391
Size: 1188 310 742 85 1071 2174
Fragments arranged by size:
2174 1188 1071 1050 972 742 600 399
310 238 180 85 36 25
BspEI T'CCGG_A
Cuts at: 8733 8733
Size: 9070
BspHI T'CATG_A
Cuts at: 3045 3657 4416 6959 8689 3045
Size: 612 759 2543 1730 3426
Fragments arranged by size:
3426 2543 1730 759 612
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2644 2973 3956 6425 844
Size: 1800 329 983 2469 3489
Fragments arranged by size:
3489 2469 1800 983 329
BsrBI CCG'CTC
Cuts at: 1394 1823 6957 1394
Size: 429 5134 3507
Fragments arranged by size:
5134 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2424 2479 2878 4907 5497 7565 7739
Size: 1183 1176 55 399 2029 590 2068 174
Cuts at: 7739 65
Size: 1396
Fragments arranged by size:
2068 2029 1396 1183 1176 590 399 174
55
BsrFI r'CCGG_y
Cuts at: 1700 1810 7714 7901 1700
Size: 110 5904 187 2869
Fragments arranged by size:
5904 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 5217 96
Size: 5121 3949
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9070
BssSI C'ACGA_G
Cuts at: 7130 8142 7130
Size: 1012 8058
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4082 4850 794
Size: 3288 768 5014
Fragments arranged by size:
5014 3288 768
BstBI TT'CG_AA
Cuts at: 2007 7874 2007
Size: 5867 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9070
BstYI r'GATC_y
Cuts at: 1972 3393 4288 4408 4867 6400 6767 7152 7169
Size: 1421 895 120 459 1533 367 385 17
Cuts at: 7169 8621 8707 8719 9065 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1533 1452 1421 895 459 385 367
346 120 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3108 5817 3108
Size: 2709 6361
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 7561
Fragments arranged by size:
7561 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8331
Fragments arranged by size:
8331 683 56
BtsI GCAGTG_nn'
Cuts at: 2975 5816 6586 7380 7400 2975
Size: 2841 770 794 20 4645
Fragments arranged by size:
4645 2841 794 770 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9070
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9035
DraI TTT'AAA
Cuts at: 2193 2652 2860 3480 6374 2193
Size: 459 208 620 2894 4889
Fragments arranged by size:
4889 2894 620 459 208
DraIII CAC_nnn'GTG
Cuts at: 5226 6064 5226
Size: 838 8232
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4191 8077 809
Size: 507 2875 3886 1802
Fragments arranged by size:
3886 2875 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2524 6517 7406
Size: 54 1425 50 275 18 694 3993 889
Cuts at: 7406 9035 8
Size: 1629 43
Fragments arranged by size:
3993 1629 1425 889 694 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6517 1537
Size: 275 4705 4090
Fragments arranged by size:
4705 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2684 4116 4879 4983 5928 7000 1353
Size: 1331 1432 763 104 945 1072 3423
Fragments arranged by size:
3423 1432 1331 1072 945 763 104
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2058 2999 5862 7657 8031 8177
Size: 1064 138 469 941 2863 1795 374 146
Cuts at: 8177 387
Size: 1280
Fragments arranged by size:
2863 1795 1280 1064 941 469 374 146
138
EcoICRI GAG'CTC
Cuts at: 2568 2806 4889 2568
Size: 238 2083 6749
Fragments arranged by size:
6749 2083 238
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2190 2199 2322 2708 3771 4961 5775
Size: 393 558 9 123 386 1063 1190 814
Cuts at: 5775 5970 7133 7730 8517 8785 1239
Size: 195 1163 597 787 268 1524
Fragments arranged by size:
1524 1190 1163 1063 814 787 597 558
393 386 268 195 123 9
EcoO109I rG'GnC_Cy
Cuts at: 4184 4184
Size: 9070
EcoRI G'AATT_C
Cuts at: 6379 6757 6379
Size: 378 8692
EcoRV GAT'ATC
Cuts at: 2995 2995
Size: 9070
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3850 5099 3850
Size: 1249 7821
FspI TGC'GCA
Cuts at: 7576 7576
Size: 9070
HaeII r_GCGC'y
Cuts at: 1678 2890 8217 1678
Size: 1212 5327 2531
Fragments arranged by size:
5327 2531 1212
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2084 2116 2150 2182 2281
Size: 32 135 32 512 32 34 32 99
Cuts at: 2281 2313 2579 2611 3385 3417 3854 3886 4273
Size: 32 266 32 774 32 437 32 387
Cuts at: 4273 4305 7711 7743 7785 7817 1373
Size: 32 3406 32 42 32 2626
Fragments arranged by size:
3406 2626 774 512 437 387 266 135
99 42 34 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5425 6414 6660 669
Size: 988 281 3487 989 246 3079
Fragments arranged by size:
3487 3079 989 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9070
HpaI GTT'AAC
Cuts at: 5425 6660 5425
Size: 1235 7835
KpnI G_GTAC'C
Cuts at: 2636 6395 2636
Size: 3759 5311
MfeI C'AATT_G
Cuts at: 6111 6669 6111
Size: 558 8512
MluI A'CGCG_T
Cuts at: 8946 8946
Size: 9070
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3553 3752 3968 5750 8184 8368 1305
Size: 2248 199 216 1782 2434 184 2007
Fragments arranged by size:
2434 2248 2007 1782 216 199 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2526 10
Size: 54 1425 343 694 6554
Fragments arranged by size:
6554 1425 694 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3674 4602 7028 7387 7546 518
Size: 648 2508 928 2426 359 159 2042
Fragments arranged by size:
2508 2426 2042 928 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3152 3296 4955 6486 7163 8311 8556 1606
Size: 1546 144 1659 1531 677 1148 245 2120
Fragments arranged by size:
2120 1659 1546 1531 1148 677 245 144
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 7561
Fragments arranged by size:
7561 957 552
NdeI CA'TA_TG
Cuts at: 387 2450 2544 2665 387
Size: 2063 94 121 6792
Fragments arranged by size:
6792 2063 121 94
NheI G'CTAG_C
Cuts at: 1052 3032 1052
Size: 1980 7090
NotI GC'GGCC_GC
Cuts at: 6517 6517
Size: 9070
NspI r_CATG'y
Cuts at: 1132 1229 2786 3735 4952 6428 7973 1132
Size: 97 1557 949 1217 1476 1545 2229
Fragments arranged by size:
2229 1557 1545 1476 1217 949 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9070
PciI A'CATG_T
Cuts at: 7969 7969
Size: 9070
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 9070
PfoI T'CCnGG_A
Cuts at: 3813 5386 5521 3813
Size: 1573 135 7362
Fragments arranged by size:
7362 1573 135
PmeI GTTT'AAAC
Cuts at: 6374 6374
Size: 9070
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2011 2043 7139 7171 8652 8684 2011
Size: 32 5096 32 1481 32 2397
Fragments arranged by size:
5096 2397 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4184 4184
Size: 9070
PshAI GACnn'nnGTC
Cuts at: 1613 4490 1613
Size: 2877 6193
PsiI TTA'TAA
Cuts at: 2351 2516 3270 6640 2351
Size: 165 754 3370 4781
Fragments arranged by size:
4781 3370 754 165
PstI C_TGCA'G
Cuts at: 830 6422 830
Size: 5592 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9070
PvuII CAG'CTG
Cuts at: 3152 3296 4955 3152
Size: 144 1659 7267
Fragments arranged by size:
7267 1659 144
SacI G_AGCT'C
Cuts at: 2570 2808 4891 2570
Size: 238 2083 6749
Fragments arranged by size:
6749 2083 238
SalI G'TCGA_C
Cuts at: 1936 6412 1936
Size: 4476 4594
SapI GCTCTTCn'nnn_
Cuts at: 4879 5928 4879
Size: 1049 8021
SbfI CC_TGCA'GG
Cuts at: 6422 6422
Size: 9070
ScaI AGT'ACT
Cuts at: 3717 5263 7318 9030 3717
Size: 1546 2055 1712 3757
Fragments arranged by size:
3757 2055 1712 1546
SfcI C'TryA_G
Cuts at: 826 1045 3432 6321 6418 7553 8234 8425 826
Size: 219 2387 2889 97 1135 681 191 1471
Fragments arranged by size:
2889 2387 1471 1135 681 219 191 97
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9070
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9070
SmaI CCC'GGG
Cuts at: 1872 6397 1872
Size: 4525 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2228 2417 2700 3980 4573 4832
Size: 197 926 285 189 283 1280 593 259
Cuts at: 4832 7174 8075 8337 820
Size: 2342 901 262 1553
Fragments arranged by size:
2342 1553 1280 926 901 593 285 283
262 259 197 189
SnaBI TAC'GTA
Cuts at: 493 6367 6861 493
Size: 5874 494 2702
Fragments arranged by size:
5874 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9070
SphI G_CATG'C
Cuts at: 1132 4952 6428 1132
Size: 3820 1476 3774
Fragments arranged by size:
3820 3774 1476
SspI AAT'ATT
Cuts at: 5 52 2815 4593 5103 5161 6994 5
Size: 47 2763 1778 510 58 1833 2081
Fragments arranged by size:
2763 2081 1833 1778 510 58 47
StuI AGG'CCT
Cuts at: 1517 4809 1517
Size: 3292 5778
StyI C'CwwG_G
Cuts at: 513 1065 2022 5182 6509 513
Size: 552 957 3160 1327 3074
Fragments arranged by size:
3160 3074 1327 957 552
SwaI ATTT'AAAT
Cuts at: 2860 3480 2860
Size: 620 8450
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2501 7097 7114 7267 7452 7897 325
Size: 1255 921 4596 17 153 185 445 1498
Fragments arranged by size:
4596 1498 1255 921 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3715 5044 5217
Size: 274 80 33 51 1443 1738 1329 173
Cuts at: 5217 5261 7316 9028 96
Size: 44 2055 1712 138
Fragments arranged by size:
2055 1738 1712 1443 1329 274 173 138
80 51 44 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5599 5631 9030 9062 5599
Size: 32 3399 32 5607
Fragments arranged by size:
5607 3399 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9070
XbaI T'CTAG_A
Cuts at: 6406 6406
Size: 9070
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9070
XmaI C'CCGG_G
Cuts at: 1870 6395 1870
Size: 4525 4545
XmnI GAAnn'nnTTC
Cuts at: 3665 7199 3665
Size: 3534 5536
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6878 276
Size: 53 83 186 936 5344 2468
Fragments arranged by size:
5344 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BstXI BstZ17I EcoNI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI
SfoI SrfI XcmI