Restriction map for
pFN21ASDA0977
(Circular) (Six-base) MAPSORT of: pFN21ASDA0977.seq Check: 8703 from: 1 to: 8227
pFN21ASDA0977 8227 bp
With 182 enzymes: SgfI *
July 22, 2011 10:10 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6037 278
Size: 53 83 186 936 4501 2468
Fragments arranged by size:
4501 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5570 1937
Size: 3633 4594
Acc65I G'GTAC_C
Cuts at: 5548 5548
Size: 8227
AclI AA'CG_TT
Cuts at: 1563 6354 6727 1563
Size: 4791 373 3063
Fragments arranged by size:
4791 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2109 3195 4989 5060 6290 7674 7942 1632
Size: 477 1086 1794 71 1230 1384 268 1917
Fragments arranged by size:
1917 1794 1384 1230 1086 477 268 71
AflII C'TTAA_G
Cuts at: 820 1017 4069 4891 820
Size: 197 3052 822 4156
Fragments arranged by size:
4156 3052 822 197
AflIII A'CryG_T
Cuts at: 1203 7126 8103 1203
Size: 5923 977 1327
Fragments arranged by size:
5923 1327 977
AgeI A'CCGG_T
Cuts at: 7058 7058
Size: 8227
AhdI GACnn_n'nnGTC
Cuts at: 6956 6956
Size: 8227
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8227
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3303 1184
Size: 2119 6108
AlwNI CAG_nnn'CTG
Cuts at: 2132 5970 7542 2132
Size: 3838 1572 2817
Fragments arranged by size:
3838 2817 1572
ApaI G_GGCC'C
Cuts at: 3680 3680
Size: 8227
ApaLI G'TGCA_C
Cuts at: 2286 3283 4170 6284 7440 2286
Size: 997 887 2114 1156 3073
Fragments arranged by size:
3073 2114 1156 997 887
ApoI r'AATT_y
Cuts at: 1493 2354 3082 4103 4219 4657 4691 5222 5287
Size: 861 728 1021 116 438 34 531 65
Cuts at: 5287 5536 5762 5914 8023 1493
Size: 249 226 152 2109 1697
Fragments arranged by size:
2109 1697 1021 861 728 531 438 249
226 152 116 65 34
AseI AT'TA_AT
Cuts at: 160 1032 2475 6781 160
Size: 872 1443 4306 1606
Fragments arranged by size:
4306 1606 1443 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8227
AvaI C'yCGr_G
Cuts at: 1870 1943 4701 5552 1870
Size: 73 2758 851 4545
Fragments arranged by size:
4545 2758 851 73
BamHI G'GATC_C
Cuts at: 5557 5924 5557
Size: 367 7860
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2083 4118 5060 5548 7003
Size: 325 217 627 296 2035 942 488 1455
Cuts at: 7003 618
Size: 1842
Fragments arranged by size:
2035 1842 1455 942 627 488 325 296
217
BanII G_rGCy'C
Cuts at: 1391 2908 3680 4577 5546 1391
Size: 1517 772 897 969 4072
Fragments arranged by size:
4072 1517 969 897 772
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2501 3738 5048 5210 928
Size: 971 602 1237 1310 162 3945
Fragments arranged by size:
3945 1310 1237 971 602 162
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5388 5422 6418 6452 1903
Size: 34 3451 34 996 34 3678
Fragments arranged by size:
3678 3451 996 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5035 6119 7329 5035
Size: 1084 1210 5933
Fragments arranged by size:
5933 1210 1084
BclI T'GATC_A
Cuts at: 1548 4408 1548
Size: 2860 5367
BfrBI ATG'CAT
Cuts at: 2522 4420 2522
Size: 1898 6329
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6838 136
Size: 107 122 71 631 605 5166 1525
Fragments arranged by size:
5166 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8222 8222
Size: 8227
BlpI GC'TnA_GC
Cuts at: 3062 5644 3062
Size: 2582 5645
Bme1580I G_kGCm'C
Cuts at: 1790 2290 2794 3287 3329 3680 4174 4563 5065
Size: 500 504 493 42 351 494 389 502
Cuts at: 5065 6288 7444 1790
Size: 1223 1156 2573
Fragments arranged by size:
2573 1223 1156 504 502 500 494 493
389 351 42
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8227
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3912 4557 5958
Size: 416 53 83 399 24 2495 645 1401
Cuts at: 5958 6916 442
Size: 958 1753
Fragments arranged by size:
2495 1753 1401 958 645 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8227
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2922 3328 2922
Size: 406 7821
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2079 3183 5166 6887 1239
Size: 840 1104 1983 1721 2579
Fragments arranged by size:
2579 1983 1721 1104 840
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4081 5062 6352 7217 7515 4081
Size: 981 1290 865 298 4793
Fragments arranged by size:
4793 1290 981 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2096 2769 3811 6890 882
Size: 617 597 673 1042 3079 2219
Fragments arranged by size:
3079 2219 1042 673 617 597
BsaAI yAC'GTr
Cuts at: 493 1204 5524 6018 493
Size: 711 4320 494 2702
Fragments arranged by size:
4320 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 8221 8221
Size: 8227
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2052 6034 6416 7051
Size: 53 83 186 936 519 3982 382 635
Cuts at: 7051 275
Size: 1451
Fragments arranged by size:
3982 1451 936 635 519 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4870 6660 7058 7332 7479 7890 4870
Size: 1790 398 274 147 411 5207
Fragments arranged by size:
5207 1790 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4870 4900 1178
Size: 30 165 30 3467 30 4505
Fragments arranged by size:
4505 3467 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3245 4971 1186
Size: 2059 1726 4442
Fragments arranged by size:
4442 2059 1726
BseYI C'CCAG_C
Cuts at: 2056 4727 7430 7926 2056
Size: 2671 2703 496 2357
Fragments arranged by size:
2703 2671 2357 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3942 5256 1163
Size: 573 2206 1314 4134
Fragments arranged by size:
4134 2206 1314 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2032 5677 6438 7466 1540
Size: 275 186 31 3645 761 1028 2301
Fragments arranged by size:
3645 2301 1028 761 275 186 31
BsiHKAI G_wGCw'C
Cuts at: 2290 3287 4174 4577 6288 6373 7444 2290
Size: 997 887 403 1711 85 1071 3073
Fragments arranged by size:
3073 1711 1071 997 887 403 85
BsmI GAATG_Cn'
Cuts at: 5738 5831 5738
Size: 93 8134
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2290 2794 2908 3287 3329 3680 4174
Size: 399 500 504 114 379 42 351 494
Cuts at: 4174 4563 4577 5065 5546 6288 6373 7444 1391
Size: 389 14 488 481 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 504 500 494 488 481
399 389 379 351 114 85 42 14
BspEI T'CCGG_A
Cuts at: 4870 7890 4870
Size: 3020 5207
BspHI T'CATG_A
Cuts at: 4444 6116 7846 4444
Size: 1672 1730 4825
Fragments arranged by size:
4825 1730 1672
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3038 4618 5582 844
Size: 2194 1580 964 3489
Fragments arranged by size:
3489 2194 1580 964
BsrBI CCG'CTC
Cuts at: 1394 1823 6114 1394
Size: 429 4291 3507
Fragments arranged by size:
4291 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3538 3637 6722 6896 65
Size: 1183 2290 99 3085 174 1396
Fragments arranged by size:
3085 2290 1396 1183 174 99
BsrFI r'CCGG_y
Cuts at: 1700 1810 6871 7058 1700
Size: 110 5061 187 2869
Fragments arranged by size:
5061 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8227
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8227
BssSI C'ACGA_G
Cuts at: 4302 6287 7299 4302
Size: 1985 1012 5230
Fragments arranged by size:
5230 1985 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4558 794
Size: 3764 4463
BstBI TT'CG_AA
Cuts at: 2007 7031 2007
Size: 5024 3203
BstEII G'GTnAC_C
Cuts at: 1184 5437 1184
Size: 4253 3974
BstXI CCAn_nnnn'nTGG
Cuts at: 3001 3301 4360 5170 3001
Size: 300 1059 810 6058
Fragments arranged by size:
6058 1059 810 300
BstYI r'GATC_y
Cuts at: 1972 4141 4159 5557 5924 6309 6326 7778 7864
Size: 2169 18 1398 367 385 17 1452 86
Cuts at: 7864 7876 8222 1972
Size: 12 346 1977
Fragments arranged by size:
2169 1977 1452 1398 385 367 346 86
18 17 12
Bsu36I CC'TnA_GG
Cuts at: 3228 3339 3228
Size: 111 8116
BtgI C'CryG_G
Cuts at: 513 1065 2022 3561 4590 513
Size: 552 957 1539 1029 4150
Fragments arranged by size:
4150 1539 1029 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2508 2681 487
Size: 683 56 1282 173 6033
Fragments arranged by size:
6033 1282 683 173 56
BtsI GCAGTG_nn'
Cuts at: 2148 2909 3053 3285 3287 4224 4511 5743 6537
Size: 761 144 232 2 937 287 1232 794
Cuts at: 6537 6557 2148
Size: 20 3818
Fragments arranged by size:
3818 1232 937 794 761 287 232 144
20 2
ClaI AT'CG_AT
Cuts at: 1554 2322 1554
Size: 768 7459
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3520 3555 529
Size: 35 2956 35 5201
Fragments arranged by size:
5201 2956 35 35
DraI TTT'AAA
Cuts at: 2406 4696 5531 2406
Size: 2290 835 5102
Fragments arranged by size:
5102 2290 835
DraIII CAC_nnn'GTG
Cuts at: 3611 4774 5159 3611
Size: 1163 385 6679
Fragments arranged by size:
6679 1163 385
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7234 809
Size: 507 5918 1802
Fragments arranged by size:
5918 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2029 5515 5674
Size: 54 1425 50 275 18 199 3486 159
Cuts at: 5674 6563 8192 8
Size: 889 1629 43
Fragments arranged by size:
3486 1629 1425 889 275 199 159 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2029 5674 1537
Size: 275 217 3645 4090
Fragments arranged by size:
4090 3645 275 217
EarI CTCTTCn'nnn_
Cuts at: 1353 2128 2891 3031 3387 4500 4748 6157 1353
Size: 775 763 140 356 1113 248 1409 3423
Fragments arranged by size:
3423 1409 1113 775 763 356 248 140
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6814 7188 7334 387
Size: 1064 138 5225 374 146 1280
Fragments arranged by size:
5225 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4575 4575
Size: 8227
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2079 2109 3183 3195 4989 5060 5166
Size: 393 447 30 1074 12 1794 71 106
Cuts at: 5166 6290 6887 7674 7942 1239
Size: 1124 597 787 268 1524
Fragments arranged by size:
1794 1524 1124 1074 787 597 447 393
268 106 71 30 12
EcoRI G'AATT_C
Cuts at: 3082 4103 5287 5536 5914 3082
Size: 1021 1184 249 378 5395
Fragments arranged by size:
5395 1184 1021 378 249
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4163 4163
Size: 8227
FspI TGC'GCA
Cuts at: 6733 6733
Size: 8227
HaeII r_GCGC'y
Cuts at: 1678 7374 1678
Size: 5696 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2315 2347 2497 2529 2588
Size: 32 135 32 743 32 150 32 59
Cuts at: 2588 2617 2620 2649 3221 3253 3503 3535 3806
Size: 29 3 29 572 32 250 32 271
Cuts at: 3806 3838 4367 4399 4870 4902 5303 5335 6868
Size: 32 529 32 471 32 401 32 1533
Cuts at: 6868 6900 6942 6974 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1533 743 572 529 471 401 271
250 150 135 59 42 32 32 32
32 32 32 32 32 32 32 32
32 29 29 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2677 3649 5571 5817 669
Size: 988 281 739 972 1922 246 3079
Fragments arranged by size:
3079 1922 988 972 739 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8227
HpaI GTT'AAC
Cuts at: 5817 5817
Size: 8227
KpnI G_GTAC'C
Cuts at: 5552 5552
Size: 8227
MfeI C'AATT_G
Cuts at: 5826 5826
Size: 8227
MluI A'CGCG_T
Cuts at: 8103 8103
Size: 8227
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2364 2400 2862 2892 5200 5330 7341 7525
Size: 1059 36 462 30 2308 130 2011 184
Cuts at: 7525 1305
Size: 2007
Fragments arranged by size:
2308 2011 2007 1059 462 184 130 36
30
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6405
Fragments arranged by size:
6405 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2999 3147 3803 4179 4443 4449 5330
Size: 648 1833 148 656 376 264 6 881
Cuts at: 5330 6185 6544 6703 518
Size: 855 359 159 2042
Fragments arranged by size:
2042 1833 881 855 656 648 376 359
264 159 148 6
MspA1I CmG'CkG
Cuts at: 1606 2099 2129 2336 4787 5643 6320 7468 7713
Size: 493 30 207 2451 856 677 1148 245
Cuts at: 7713 1606
Size: 2120
Fragments arranged by size:
2451 2120 1148 856 677 493 245 207
30
NcoI C'CATG_G
Cuts at: 513 1065 2022 3561 4590 513
Size: 552 957 1539 1029 4150
Fragments arranged by size:
4150 1539 1029 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8227
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8227
NotI GC'GGCC_GC
Cuts at: 5674 5674
Size: 8227
NsiI A_TGCA'T
Cuts at: 2524 4422 2524
Size: 1898 6329
NspI r_CATG'y
Cuts at: 1132 1229 2522 3073 4420 4424 5585 7130 1132
Size: 97 1293 551 1347 4 1161 1545 2229
Fragments arranged by size:
2229 1545 1347 1293 1161 551 97 4
PasI CC'CwG_GG
Cuts at: 1348 3260 3329 1348
Size: 1912 69 6246
Fragments arranged by size:
6246 1912 69
PciI A'CATG_T
Cuts at: 7126 7126
Size: 8227
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3188 5243 5312 1108
Size: 2080 2055 69 4023
Fragments arranged by size:
4023 2080 2055 69
PfoI T'CCnGG_A
Cuts at: 2961 2961
Size: 8227
PmeI GTTT'AAAC
Cuts at: 5531 5531
Size: 8227
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3034 3066 5326 5358 6296 6328 7809 7841 3034
Size: 32 2260 32 938 32 1481 32 3420
Fragments arranged by size:
3420 2260 1481 938 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8227
PsiI TTA'TAA
Cuts at: 4338 5797 4338
Size: 1459 6768
PspOMI G'GGCC_C
Cuts at: 3676 3676
Size: 8227
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3177 3209 3177
Size: 32 8195
PstI C_TGCA'G
Cuts at: 830 3060 3079 4786 5579 830
Size: 2230 19 1707 793 3478
Fragments arranged by size:
3478 2230 1707 793 19
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8227
PvuII CAG'CTG
Cuts at: 2099 2129 2336 4787 2099
Size: 30 207 2451 5539
Fragments arranged by size:
5539 2451 207 30
SacI G_AGCT'C
Cuts at: 4577 4577
Size: 8227
SalI G'TCGA_C
Cuts at: 1936 5569 1936
Size: 3633 4594
SapI GCTCTTCn'nnn_
Cuts at: 4500 4500
Size: 8227
SbfI CC_TGCA'GG
Cuts at: 5579 5579
Size: 8227
ScaI AGT'ACT
Cuts at: 6475 8187 6475
Size: 1712 6515
SfcI C'TryA_G
Cuts at: 826 1045 2139 3056 3075 4200 4782 5109 5575
Size: 219 1094 917 19 1125 582 327 466
Cuts at: 5575 6710 7391 7582 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1125 1094 917 681 582 466
327 219 191 19
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8227
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8227
SmaI CCC'GGG
Cuts at: 1872 5554 1872
Size: 3682 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4069 4096 4891 5077 6331 7232
Size: 197 926 2126 27 795 186 1254 901
Cuts at: 7232 7494 820
Size: 262 1553
Fragments arranged by size:
2126 1553 1254 926 901 795 262 197
186 27
SnaBI TAC'GTA
Cuts at: 493 5524 6018 493
Size: 5031 494 2702
Fragments arranged by size:
5031 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8227
SphI G_CATG'C
Cuts at: 1132 4424 5585 1132
Size: 3292 1161 3774
Fragments arranged by size:
3774 3292 1161
SspI AAT'ATT
Cuts at: 5 52 3448 4678 6151 5
Size: 47 3396 1230 1473 2081
Fragments arranged by size:
3396 2081 1473 1230 47
StuI AGG'CCT
Cuts at: 1517 2971 1517
Size: 1454 6773
StyI C'CwwG_G
Cuts at: 513 1065 2022 2079 3526 3561 4359 4590 5666
Size: 552 957 57 1447 35 798 231 1076
Cuts at: 5666 513
Size: 3074
Fragments arranged by size:
3074 1447 1076 957 798 552 231 57
35
SwaI ATTT'AAAT
Cuts at: 4696 4696
Size: 8227
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2905 4943 6254 6271 6424 6609 7054
Size: 1255 1325 2038 1311 17 153 185 445
Cuts at: 7054 325
Size: 1498
Fragments arranged by size:
2038 1498 1325 1311 1255 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6473 8185 96
Size: 274 80 33 51 1443 4496 1712 138
Fragments arranged by size:
4496 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8187 8219 8187
Size: 32 8195
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8227
XbaI T'CTAG_A
Cuts at: 5563 5563
Size: 8227
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8227
XmaI C'CCGG_G
Cuts at: 1870 5552 1870
Size: 3682 4545
XmnI GAAnn'nnTTC
Cuts at: 3252 6356 3252
Size: 3104 5123
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6035 276
Size: 53 83 186 936 4501 2468
Fragments arranged by size:
4501 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BsmBI BstZ17I EcoNI EcoO109I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PpuMI PspXI RsrII SacII SanDI SexAI SfiI SfoI
SrfI XcmI