Restriction map for
pFN21ASDA0976
(Circular) (Six-base) MAPSORT of: pFN21ASDA0976.seq Check: 4616 from: 1 to: 5821
pFN21ASDA0976 5821 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3631 278
Size: 53 83 186 936 2095 2468
Fragments arranged by size:
2468 2095 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2663 3164 1937
Size: 726 501 4594
Fragments arranged by size:
4594 726 501
Acc65I G'GTAC_C
Cuts at: 3142 3142
Size: 5821
AclI AA'CG_TT
Cuts at: 1563 3948 4321 1563
Size: 2385 373 3063
Fragments arranged by size:
3063 2385 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3884 5268 5536 1632
Size: 2252 1384 268 1917
Fragments arranged by size:
2252 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5624
AflIII A'CryG_T
Cuts at: 1203 2280 4720 5697 1203
Size: 1077 2440 977 1327
Fragments arranged by size:
2440 1327 1077 977
AgeI A'CCGG_T
Cuts at: 4652 4652
Size: 5821
AhdI GACnn_n'nnGTC
Cuts at: 4550 4550
Size: 5821
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5821
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5821
AlwNI CAG_nnn'CTG
Cuts at: 3564 5136 3564
Size: 1572 4249
ApaLI G'TGCA_C
Cuts at: 3878 5034 3878
Size: 1156 4665
ApoI r'AATT_y
Cuts at: 1493 3130 3356 3508 5617 1493
Size: 1637 226 152 2109 1697
Fragments arranged by size:
2109 1697 1637 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4375 160
Size: 872 3343 1606
Fragments arranged by size:
3343 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
AvaI C'yCGr_G
Cuts at: 1870 1943 3146 1870
Size: 73 1203 4545
Fragments arranged by size:
4545 1203 73
BamHI G'GATC_C
Cuts at: 2587 3151 3518 2587
Size: 564 367 4890
Fragments arranged by size:
4890 564 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3142 4597 618
Size: 325 217 627 1355 1455 1842
Fragments arranged by size:
1842 1455 1355 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3140 1391
Size: 1749 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2362 928
Size: 971 463 4387
Fragments arranged by size:
4387 971 463
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2892 2926 4012 4046 1903
Size: 34 955 34 1086 34 3678
Fragments arranged by size:
3678 1086 955 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2409 2758 3095 3713 4923 2409
Size: 349 337 618 1210 3307
Fragments arranged by size:
3307 1210 618 349 337
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5821
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4432 136
Size: 107 122 71 631 605 2760 1525
Fragments arranged by size:
2760 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5816 5816
Size: 5821
BlpI GC'TnA_GC
Cuts at: 3238 3238
Size: 5821
Bme1580I G_kGCm'C
Cuts at: 1790 3882 5038 1790
Size: 2092 1156 2573
Fragments arranged by size:
2573 2092 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5821
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3084 3552 4510
Size: 416 53 83 399 24 1667 468 958
Cuts at: 4510 442
Size: 1753
Fragments arranged by size:
1753 1667 958 468 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5821
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2396 2528 4481 1239
Size: 1157 132 1953 2579
Fragments arranged by size:
2579 1953 1157 132
Bpu10I CC'TnA_GC
Cuts at: 2313 2331 2313
Size: 18 5803
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3946 4811 5109 3946
Size: 865 298 4658
Fragments arranged by size:
4658 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2235 4484 882
Size: 617 736 2249 2219
Fragments arranged by size:
2249 2219 736 617
BsaAI yAC'GTr
Cuts at: 493 1204 3118 3612 493
Size: 711 1914 494 2702
Fragments arranged by size:
2702 1914 711 494
BsaBI GATnn'nnATC
Cuts at: 5815 5815
Size: 5821
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2488 3628 4010 4645
Size: 53 83 186 936 955 1140 382 635
Cuts at: 4645 275
Size: 1451
Fragments arranged by size:
1451 1140 955 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2224 4254 4652 4926 5073 5484 2224
Size: 2030 398 274 147 411 2561
Fragments arranged by size:
2561 2030 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5596
Fragments arranged by size:
5596 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5821
BseYI C'CCAG_C
Cuts at: 5024 5520 5024
Size: 496 5325
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5248
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3271 4032 5060 1540
Size: 275 186 1270 761 1028 2301
Fragments arranged by size:
2301 1270 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2979 3882 3967 5038 2979
Size: 903 85 1071 3762
Fragments arranged by size:
3762 1071 903 85
BsmI GAATG_Cn'
Cuts at: 3332 3425 3332
Size: 93 5728
BsmBI CGTCTCn'nnnn_
Cuts at: 2246 2246
Size: 5821
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2979 3140 3882 3967 5038 1391
Size: 399 1189 161 742 85 1071 2174
Fragments arranged by size:
2174 1189 1071 742 399 161 85
BspEI T'CCGG_A
Cuts at: 2224 5484 2224
Size: 3260 2561
BspHI T'CATG_A
Cuts at: 3710 5440 3710
Size: 1730 4091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3176 844
Size: 2332 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3708 1394
Size: 429 1885 3507
Fragments arranged by size:
3507 1885 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2271 4316 4490 65
Size: 1183 1023 2045 174 1396
Fragments arranged by size:
2045 1396 1183 1023 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4465 4652 1700
Size: 110 2655 187 2869
Fragments arranged by size:
2869 2655 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5821
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5821
BssSI C'ACGA_G
Cuts at: 3881 4893 3881
Size: 1012 4809
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5821
BstBI TT'CG_AA
Cuts at: 2007 4625 2007
Size: 2618 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5821
BstYI r'GATC_y
Cuts at: 1972 2587 3151 3518 3903 3920 5372 5458 5470
Size: 615 564 367 385 17 1452 86 12
Cuts at: 5470 5816 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 615 564 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2664 2664
Size: 5821
Bsu36I CC'TnA_GG
Cuts at: 2807 2807
Size: 5821
BtgI C'CryG_G
Cuts at: 513 1065 2184 513
Size: 552 1119 4150
Fragments arranged by size:
4150 1119 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5082
Fragments arranged by size:
5082 683 56
BtsI GCAGTG_nn'
Cuts at: 3069 3337 4131 4151 3069
Size: 268 794 20 4739
Fragments arranged by size:
4739 794 268 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5821
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5786
DraI TTT'AAA
Cuts at: 3125 3125
Size: 5821
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3036 4828 809
Size: 507 1720 1792 1802
Fragments arranged by size:
1802 1792 1720 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3268 4157 5786
Size: 54 1425 50 275 18 1438 889 1629
Cuts at: 5786 8
Size: 43
Fragments arranged by size:
1629 1438 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3268 1537
Size: 275 1456 4090
Fragments arranged by size:
4090 1456 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2640 3751 1353
Size: 1287 1111 3423
Fragments arranged by size:
3423 1287 1111
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4408 4782 4928 387
Size: 1064 138 2819 374 146 1280
Fragments arranged by size:
2819 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2396 2528 3884 4481 5268 5536 1239
Size: 393 764 132 1356 597 787 268 1524
Fragments arranged by size:
1524 1356 787 764 597 393 268 132
EcoRI G'AATT_C
Cuts at: 3130 3508 3130
Size: 378 5443
FspI TGC'GCA
Cuts at: 4327 4327
Size: 5821
HaeII r_GCGC'y
Cuts at: 1678 4968 1678
Size: 3290 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2185 2217 2579 2611 4462
Size: 32 135 32 613 32 362 32 1851
Cuts at: 4462 4494 4536 4568 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1851 613 362 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2419 3165 3411 669
Size: 988 281 481 746 246 3079
Fragments arranged by size:
3079 988 746 481 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5821
HpaI GTT'AAC
Cuts at: 2419 3411 2419
Size: 992 4829
KpnI G_GTAC'C
Cuts at: 3146 3146
Size: 5821
MfeI C'AATT_G
Cuts at: 3420 3420
Size: 5821
MluI A'CGCG_T
Cuts at: 5697 5697
Size: 5821
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4935 5119 1305
Size: 3630 184 2007
Fragments arranged by size:
3630 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3999
Fragments arranged by size:
3999 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2189 2897 2968 3779 4138 4297 518
Size: 648 1023 708 71 811 359 159 2042
Fragments arranged by size:
2042 1023 811 708 648 359 159 71
MspA1I CmG'CkG
Cuts at: 1606 2761 3237 3914 5062 5307 1606
Size: 1155 476 677 1148 245 2120
Fragments arranged by size:
2120 1155 1148 677 476 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5269
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5821
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5821
NotI GC'GGCC_GC
Cuts at: 3268 3268
Size: 5821
NspI r_CATG'y
Cuts at: 1132 1229 2284 2967 3179 4724 1132
Size: 97 1055 683 212 1545 2229
Fragments arranged by size:
2229 1545 1055 683 212 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5821
PciI A'CATG_T
Cuts at: 2280 4720 2280
Size: 2440 3381
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2373 2834 2999 1108
Size: 1265 461 165 3930
Fragments arranged by size:
3930 1265 461 165
PfoI T'CCnGG_A
Cuts at: 2505 2505
Size: 5821
PmeI GTTT'AAAC
Cuts at: 3125 3125
Size: 5821
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3890 3922 5403 5435 3890
Size: 32 1481 32 4276
Fragments arranged by size:
4276 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5821
PsiI TTA'TAA
Cuts at: 3391 3391
Size: 5821
PstI C_TGCA'G
Cuts at: 830 3173 830
Size: 2343 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5821
PvuII CAG'CTG
Cuts at: 2761 2761
Size: 5821
SalI G'TCGA_C
Cuts at: 1936 3163 1936
Size: 1227 4594
SbfI CC_TGCA'GG
Cuts at: 3173 3173
Size: 5821
ScaI AGT'ACT
Cuts at: 2651 4069 5781 2651
Size: 1418 1712 2691
Fragments arranged by size:
2691 1712 1418
SfcI C'TryA_G
Cuts at: 826 1045 2985 3169 4304 4985 5176 826
Size: 219 1940 184 1135 681 191 1471
Fragments arranged by size:
1940 1471 1135 681 219 191 184
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5821
SmaI CCC'GGG
Cuts at: 1872 3148 1872
Size: 1276 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3925 4826 5088 820
Size: 197 926 1982 901 262 1553
Fragments arranged by size:
1982 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3118 3612 493
Size: 2625 494 2702
Fragments arranged by size:
2702 2625 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5821
SphI G_CATG'C
Cuts at: 1132 3179 1132
Size: 2047 3774
SspI AAT'ATT
Cuts at: 5 52 2844 3745 5
Size: 47 2792 901 2081
Fragments arranged by size:
2792 2081 901 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5821
StyI C'CwwG_G
Cuts at: 513 1065 3032 3260 513
Size: 552 1967 228 3074
Fragments arranged by size:
3074 1967 552 228
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3848 3865 4018 4203 4648 325
Size: 1255 2268 17 153 185 445 1498
Fragments arranged by size:
2268 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2649 4067 5779
Size: 274 80 33 51 1443 672 1418 1712
Cuts at: 5779 96
Size: 138
Fragments arranged by size:
1712 1443 1418 672 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2974 3006 5781 5813 2974
Size: 32 2775 32 2982
Fragments arranged by size:
2982 2775 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5821
XbaI T'CTAG_A
Cuts at: 3157 3157
Size: 5821
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5821
XmaI C'CCGG_G
Cuts at: 1870 3146 1870
Size: 1276 4545
XmnI GAAnn'nnTTC
Cuts at: 3950 3950
Size: 5821
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3629 276
Size: 53 83 186 936 2095 2468
Fragments arranged by size:
2468 2095 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BstXI DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI