Restriction map for
pFN21ASDA0975
(Circular) (Six-base) MAPSORT of: pFN21ASDA0975.seq Check: 1166 from: 1 to: 9241
pFN21ASDA0975 9241 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3803 5770 6421 3803
Size: 1967 651 6623
Fragments arranged by size:
6623 1967 651
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7051 278
Size: 53 83 186 936 5515 2468
Fragments arranged by size:
5515 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2304 4596 4605 5013 6584 1937
Size: 367 2292 9 408 1571 4594
Fragments arranged by size:
4594 2292 1571 408 367 9
Acc65I G'GTAC_C
Cuts at: 4953 6234 6562 4953
Size: 1281 328 7632
Fragments arranged by size:
7632 1281 328
AclI AA'CG_TT
Cuts at: 1563 7368 7741 1563
Size: 5805 373 3063
Fragments arranged by size:
5805 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2433 2823 2853 5066 5253 7304 8688 8956
Size: 801 390 30 2213 187 2051 1384 268
Cuts at: 8956 1632
Size: 1917
Fragments arranged by size:
2213 2051 1917 1384 801 390 268 187
30
AflII C'TTAA_G
Cuts at: 820 1017 2582 2591 820
Size: 197 1565 9 7470
Fragments arranged by size:
7470 1565 197 9
AflIII A'CryG_T
Cuts at: 1203 3240 8140 9117 1203
Size: 2037 4900 977 1327
Fragments arranged by size:
4900 2037 1327 977
AgeI A'CCGG_T
Cuts at: 8072 8072
Size: 9241
AhdI GACnn_n'nnGTC
Cuts at: 5719 7970 5719
Size: 2251 6990
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9241
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2502 3894 5415 1184
Size: 1318 1392 1521 5010
Fragments arranged by size:
5010 1521 1392 1318
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5993 6025 5993
Size: 32 9209
AlwNI CAG_nnn'CTG
Cuts at: 2443 2647 3013 3710 3838 5044 5210 6984 8556
Size: 204 366 697 128 1206 166 1774 1572
Cuts at: 8556 2443
Size: 3128
Fragments arranged by size:
3128 1774 1572 1206 697 366 204 166
128
ApaI G_GGCC'C
Cuts at: 2049 3999 4102 6122 2049
Size: 1950 103 2020 5168
Fragments arranged by size:
5168 2020 1950 103
ApaLI G'TGCA_C
Cuts at: 7298 8454 7298
Size: 1156 8085
ApoI r'AATT_y
Cuts at: 1493 2813 6550 6776 6928 9037 1493
Size: 1320 3737 226 152 2109 1697
Fragments arranged by size:
3737 2109 1697 1320 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7795 160
Size: 872 6763 1606
Fragments arranged by size:
6763 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9241
AvaI C'yCGr_G
Cuts at: 1870 1943 2048 2552 3614 4260 4908 5072 5341
Size: 73 105 504 1062 646 648 164 269
Cuts at: 5341 6200 6566 1870
Size: 859 366 4545
Fragments arranged by size:
4545 1062 859 648 646 504 366 269
164 105 73
BamHI G'GATC_C
Cuts at: 6571 6938 6571
Size: 367 8874
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3757 4814 4913 4953 5499
Size: 325 217 627 1970 1057 99 40 546
Cuts at: 5499 6234 6382 6562 8017 618
Size: 735 148 180 1455 1842
Fragments arranged by size:
1970 1842 1455 1057 735 627 546 325
217 180 148 99 40
BanII G_rGCy'C
Cuts at: 1391 2049 2093 2421 3999 4102 5099 5457 6122
Size: 658 44 328 1578 103 997 358 665
Cuts at: 6122 6528 6560 1391
Size: 406 32 4072
Fragments arranged by size:
4072 1578 997 665 658 406 358 328
103 44 32
BbeI G_GCGC'C
Cuts at: 4917 4917
Size: 9241
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3453 4228 5054 5246 6313 928
Size: 971 1554 775 826 192 1067 3856
Fragments arranged by size:
3856 1554 1067 971 826 775 192
BbvCI CC'TCA_GC
Cuts at: 5221 5475 5495 6056 5221
Size: 254 20 561 8406
Fragments arranged by size:
8406 561 254 20
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2444 2478 5312 5346 7432 7466 1903
Size: 34 507 34 2834 34 2086 34 3678
Fragments arranged by size:
3678 2834 2086 507 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4196 7133 8343 4196
Size: 2937 1210 5094
Fragments arranged by size:
5094 2937 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9241
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2066 5436 7852
Size: 107 122 71 631 605 394 3370 2416
Cuts at: 7852 136
Size: 1525
Fragments arranged by size:
3370 2416 1525 631 605 394 122 107
71
BglII A'GATC_T
Cuts at: 6159 9236 6159
Size: 3077 6164
BlpI GC'TnA_GC
Cuts at: 2709 4850 6033 6658 2709
Size: 2141 1183 625 5292
Fragments arranged by size:
5292 2141 1183 625
Bme1580I G_kGCm'C
Cuts at: 1790 2049 2559 3999 4102 6122 7302 8458 1790
Size: 259 510 1440 103 2020 1180 1156 2573
Fragments arranged by size:
2573 2020 1440 1180 1156 510 259 103
BmgBI CAC'GTC
Cuts at: 1318 3700 3967 4006 5422 6331 1318
Size: 2382 267 39 1416 909 4228
Fragments arranged by size:
4228 2382 1416 909 267 39
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5180 5329 5683
Size: 416 53 83 399 24 3763 149 354
Cuts at: 5683 5976 6438 6972 7930 442
Size: 293 462 534 958 1753
Fragments arranged by size:
3763 1753 958 534 462 416 399 354
293 149 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9241
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2456 3334 6482 2456
Size: 878 3148 5215
Fragments arranged by size:
5215 3148 878
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2294 2318 3254 4148 5187 5289 5564 5813
Size: 1055 24 936 894 1039 102 275 249
Cuts at: 5813 7901 1239
Size: 2088 2579
Fragments arranged by size:
2579 2088 1055 1039 936 894 275 249
102 24
Bpu10I CC'TnA_GC
Cuts at: 2746 2903 3839 5221 5475 5495 6056 2746
Size: 157 936 1382 254 20 561 5931
Fragments arranged by size:
5931 1382 936 561 254 157 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2248 4130 4352 7366 8231 8529 2248
Size: 1882 222 3014 865 298 2960
Fragments arranged by size:
3014 2960 1882 865 298 222
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7904 882
Size: 617 6405 2219
Fragments arranged by size:
6405 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2523 3241 4933 5790 6073 6538 7032
Size: 711 1319 718 1692 857 283 465 494
Cuts at: 7032 493
Size: 2702
Fragments arranged by size:
2702 1692 1319 857 718 711 494 465
283
BsaBI GATnn'nnATC
Cuts at: 9235 9235
Size: 9241
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4410 4914 5291 7048
Size: 53 83 186 936 2877 504 377 1757
Cuts at: 7048 7430 8065 275
Size: 382 635 1451
Fragments arranged by size:
2877 1757 1451 936 635 504 382 377
186 83 53
BsaWI w'CCGG_w
Cuts at: 3207 3282 7674 8072 8346 8493 8904 3207
Size: 75 4392 398 274 147 411 3544
Fragments arranged by size:
4392 3544 411 398 274 147 75
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2903 2933 3038 3068 3227
Size: 30 165 30 1500 30 105 30 159
Cuts at: 3227 3257 3395 3425 3490 3520 4268 4298 5885
Size: 30 138 30 65 30 748 30 1587
Cuts at: 5885 5915 6485 6515 1178
Size: 30 570 30 3904
Fragments arranged by size:
3904 1587 1500 748 570 165 159 138
105 65 30 30 30 30 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3046 3215 3584 3923 3947 3950 3959 3962
Size: 1860 169 369 339 24 3 9 3
Cuts at: 3962 3974 4025 4028 4031 4038 4058 4061 4070
Size: 12 51 3 3 7 20 3 9
Cuts at: 4070 4088 4091 4276 5300 5387 5504 6002 6005
Size: 18 3 185 1024 87 117 498 3
Cuts at: 6005 1186
Size: 4422
Fragments arranged by size:
4422 1860 1024 498 369 339 185 169
117 87 51 24 20 18 12 9
9 7 3 3 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 3082 3482 3528 4461 4764 4902 5161 5197 5233
Size: 400 46 933 303 138 259 36 36
Cuts at: 5233 5251 5317 6049 8444 8940 3082
Size: 18 66 732 2395 496 3383
Fragments arranged by size:
3383 2395 933 732 496 400 303 259
138 66 46 36 36 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3125 4196 4406 4646 4657 6095 1163
Size: 573 1389 1071 210 240 11 1438 4309
Fragments arranged by size:
4309 1438 1389 1071 573 240 210 11
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2031 4635 6691 7452 8480 1540
Size: 275 186 30 2604 2056 761 1028 2301
Fragments arranged by size:
2604 2301 2056 1028 761 275 186 30
BsiHKAI G_wGCw'C
Cuts at: 2421 3240 3621 3845 4134 4215 4368 5352 5457
Size: 819 381 224 289 81 153 984 105
Cuts at: 5457 5595 6282 6528 7302 7387 8458 2421
Size: 138 687 246 774 85 1071 3204
Fragments arranged by size:
3204 1071 984 819 774 687 381 289
246 224 153 138 105 85 81
BsmI GAATG_Cn'
Cuts at: 6752 6845 6752
Size: 93 9148
BsmBI CGTCTCn'nnnn_
Cuts at: 5282 5282
Size: 9241
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2093 2421 2559 3240 3621 3845
Size: 399 259 44 328 138 681 381 224
Cuts at: 3845 3999 4102 4134 4215 4368 5099 5352 5457
Size: 154 103 32 81 153 731 253 105
Cuts at: 5457 5595 6122 6282 6528 6560 7302 7387 8458
Size: 138 527 160 246 32 742 85 1071
Cuts at: 8458 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 731 681 527 399 381
328 259 253 246 224 160 154 153
138 138 105 103 85 81 44 32
32
BspEI T'CCGG_A
Cuts at: 3207 8904 3207
Size: 5697 3544
BspHI T'CATG_A
Cuts at: 6340 7130 8860 6340
Size: 790 1730 6721
Fragments arranged by size:
6721 1730 790
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2196 2238 3803 4428 4449 5724 5770 6421
Size: 1352 42 1565 625 21 1275 46 651
Cuts at: 6421 6596 844
Size: 175 3489
Fragments arranged by size:
3489 1565 1352 1275 651 625 175 46
42 21
BsrBI CCG'CTC
Cuts at: 1394 1823 2053 3868 4864 7128 1394
Size: 429 230 1815 996 2264 3507
Fragments arranged by size:
3507 2264 1815 996 429 230
BsrDI GCAATG_nn'
Cuts at: 65 1248 3627 5143 5469 7736 7910 65
Size: 1183 2379 1516 326 2267 174 1396
Fragments arranged by size:
2379 2267 1516 1396 1183 326 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2065 2450 6237 6530 7885 8072 1700
Size: 110 255 385 3787 293 1355 187 2869
Fragments arranged by size:
3787 2869 1355 385 293 255 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9241
BssHII G'CGCG_C
Cuts at: 1925 2033 2078 4707 1925
Size: 108 45 2629 6459
Fragments arranged by size:
6459 2629 108 45
BssSI C'ACGA_G
Cuts at: 4424 4946 4988 7301 8313 4424
Size: 522 42 2313 1012 5352
Fragments arranged by size:
5352 2313 1012 522 42
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2647 794
Size: 1853 7388
BstBI TT'CG_AA
Cuts at: 2007 8045 2007
Size: 6038 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9241
BstXI CCAn_nnnn'nTGG
Cuts at: 2353 3295 2353
Size: 942 8299
BstYI r'GATC_y
Cuts at: 1972 2293 3169 4242 5382 6159 6571 6938 7323
Size: 321 876 1073 1140 777 412 367 385
Cuts at: 7323 7340 8792 8878 8890 9236 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1140 1073 876 777 412 385
367 346 321 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 5227 5227
Size: 9241
BtgI C'CryG_G
Cuts at: 513 1065 2022 2400 4810 513
Size: 552 957 378 2410 4944
Fragments arranged by size:
4944 2410 957 552 378
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4213 5044 487
Size: 683 56 2987 831 4684
Fragments arranged by size:
4684 2987 831 683 56
BtsI GCAGTG_nn'
Cuts at: 4965 5627 5818 6194 6361 6757 7551 7571 4965
Size: 662 191 376 167 396 794 20 6635
Fragments arranged by size:
6635 794 662 396 376 191 167 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9241
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9206
DraI TTT'AAA
Cuts at: 6545 6545
Size: 9241
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8248 809
Size: 507 6932 1802
Fragments arranged by size:
6932 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2067 3579 3769
Size: 54 1425 50 275 18 237 1512 190
Cuts at: 3769 3810 4470 4632 4927 5110 6250 6505 6688
Size: 41 660 162 295 183 1140 255 183
Cuts at: 6688 7577 9206 8
Size: 889 1629 43
Fragments arranged by size:
1629 1512 1425 1140 889 660 295 275
255 237 190 183 183 162 54 50
43 41 18
EagI C'GGCC_G
Cuts at: 1537 1812 4632 6688 1537
Size: 275 2820 2056 4090
Fragments arranged by size:
4090 2820 2056 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2276 2409 3951 4035 4047 4059 4400 5462
Size: 923 133 1542 84 12 12 341 1062
Cuts at: 5462 7171 1353
Size: 1709 3423
Fragments arranged by size:
3423 1709 1542 1062 923 341 133 84
12 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2226 4178 4720 5597 7828 8202
Size: 1064 138 637 1952 542 877 2231 374
Cuts at: 8202 8348 387
Size: 146 1280
Fragments arranged by size:
2231 1952 1280 1064 877 637 542 374
146 138
EcoICRI GAG'CTC
Cuts at: 2419 5455 6526 2419
Size: 3036 1071 5134
Fragments arranged by size:
5134 3036 1071
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2294 2318 2433 2823 2853 3254 4148
Size: 393 662 24 115 390 30 401 894
Cuts at: 4148 5066 5187 5253 5289 5564 5813 7304 7901
Size: 918 121 66 36 275 249 1491 597
Cuts at: 7901 8688 8956 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1491 918 894 787 662 597 401
393 390 275 268 249 121 115 66
36 30 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2681 5357 6173 2681
Size: 2676 816 5749
Fragments arranged by size:
5749 2676 816
EcoO109I rG'GnC_Cy
Cuts at: 2612 2852 3315 3574 3995 3996 4099 5041 5158
Size: 240 463 259 421 1 103 942 117
Cuts at: 5158 5326 6001 6046 6118 6119 6265 6307 6496
Size: 168 675 45 72 1 146 42 189
Cuts at: 6496 2612
Size: 5357
Fragments arranged by size:
5357 942 675 463 421 259 240 189
168 146 117 103 72 45 42 1
1
EcoRI G'AATT_C
Cuts at: 6550 6928 6550
Size: 378 8863
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2761 4807 5017 2761
Size: 2046 210 6985
Fragments arranged by size:
6985 2046 210
FspI TGC'GCA
Cuts at: 7747 7747
Size: 9241
HaeII r_GCGC'y
Cuts at: 1678 3838 4084 4699 4917 5091 5445 8388 1678
Size: 2160 246 615 218 174 354 2943 2531
Fragments arranged by size:
2943 2531 2160 615 354 246 218 174
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2712 2744 3227 3259 3395
Size: 32 135 32 1140 32 483 32 136
Cuts at: 3395 3427 4430 4462 5705 5737 5885 5917 7882
Size: 32 1003 32 1243 32 148 32 1965
Cuts at: 7882 7914 7956 7988 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1965 1243 1140 1003 483 148 136
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3163 5887 6585 6831 669
Size: 988 281 1225 2724 698 246 3079
Fragments arranged by size:
3079 2724 1225 988 698 281 246
HindIII A'AGCT_T
Cuts at: 748 2269 5705 748
Size: 1521 3436 4284
Fragments arranged by size:
4284 3436 1521
HpaI GTT'AAC
Cuts at: 6831 6831
Size: 9241
KasI G'GCGC_C
Cuts at: 4913 4913
Size: 9241
KpnI G_GTAC'C
Cuts at: 4957 6238 6566 4957
Size: 1281 328 7632
Fragments arranged by size:
7632 1281 328
MfeI C'AATT_G
Cuts at: 6840 6840
Size: 9241
MluI A'CGCG_T
Cuts at: 9117 9117
Size: 9241
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 8355 8539 1305
Size: 7050 184 2007
Fragments arranged by size:
7050 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3581 3771 4472 4929 5112
Size: 54 1425 343 1749 190 701 457 183
Cuts at: 5112 10
Size: 4139
Fragments arranged by size:
4139 1749 1425 701 457 343 190 183
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2379 2703 4472 4976 5774 5843 7199
Size: 648 1213 324 1769 504 798 69 1356
Cuts at: 7199 7558 7717 518
Size: 359 159 2042
Fragments arranged by size:
2042 1769 1356 1213 798 648 504 359
324 159 69
MspA1I CmG'CkG
Cuts at: 1606 2402 2482 2503 3464 3874 3904 4534 4671
Size: 796 80 21 961 410 30 630 137
Cuts at: 4671 4896 5023 6657 7334 8482 8727 1606
Size: 225 127 1634 677 1148 245 2120
Fragments arranged by size:
2120 1634 1148 961 796 677 630 410
245 225 137 127 80 30 21
NaeI GCC'GGC
Cuts at: 2067 2452 2067
Size: 385 8856
NarI GG'CG_CC
Cuts at: 4914 4914
Size: 9241
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 7732
Fragments arranged by size:
7732 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9241
NgoMIV G'CCGG_C
Cuts at: 2065 2450 2065
Size: 385 8856
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9241
NotI GC'GGCC_GC
Cuts at: 6688 6688
Size: 9241
NspI r_CATG'y
Cuts at: 1132 1229 3737 6097 6599 8144 1132
Size: 97 2508 2360 502 1545 2229
Fragments arranged by size:
2508 2360 2229 1545 502 97
PasI CC'CwG_GG
Cuts at: 1348 3634 4102 5092 5537 6104 1348
Size: 2286 468 990 445 567 4485
Fragments arranged by size:
4485 2286 990 567 468 445
PciI A'CATG_T
Cuts at: 8140 8140
Size: 9241
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4468 6028 6104 1108
Size: 3360 1560 76 4245
Fragments arranged by size:
4245 3360 1560 76
PfoI T'CCnGG_A
Cuts at: 2562 2964 3174 6168 6261 2562
Size: 402 210 2994 93 5542
Fragments arranged by size:
5542 2994 402 210 93
PmeI GTTT'AAAC
Cuts at: 6545 6545
Size: 9241
PmlI CAC'GTG
Cuts at: 2523 3241 4933 5790 2523
Size: 718 1692 857 5974
Fragments arranged by size:
5974 1692 857 718
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7310 7342 8823 8855 7310
Size: 32 1481 32 7696
Fragments arranged by size:
7696 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2612 5041 5158 6001 6265 2612
Size: 2429 117 843 264 5588
Fragments arranged by size:
5588 2429 843 264 117
PshAI GACnn'nnGTC
Cuts at: 1613 2864 2973 5054 5145 1613
Size: 1251 109 2081 91 5709
Fragments arranged by size:
5709 2081 1251 109 91
PsiI TTA'TAA
Cuts at: 6811 6811
Size: 9241
PspOMI G'GGCC_C
Cuts at: 2045 3995 4098 6118 2045
Size: 1950 103 2020 5168
Fragments arranged by size:
5168 2020 1950 103
PstI C_TGCA'G
Cuts at: 830 2481 2508 3009 4518 5040 5215 5634 5721
Size: 1651 27 501 1509 522 175 419 87
Cuts at: 5721 6116 6593 830
Size: 395 477 3478
Fragments arranged by size:
3478 1651 1509 522 501 477 419 395
175 87 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9241
PvuII CAG'CTG
Cuts at: 2482 2503 3464 3874 4534 4671 4896 5023 2482
Size: 21 961 410 660 137 225 127 6700
Fragments arranged by size:
6700 961 660 410 225 137 127 21
SacI G_AGCT'C
Cuts at: 2421 5457 6528 2421
Size: 3036 1071 5134
Fragments arranged by size:
5134 3036 1071
SacII CC_GC'GG
Cuts at: 2403 2403
Size: 9241
SalI G'TCGA_C
Cuts at: 1936 6583 1936
Size: 4647 4594
SapI GCTCTTCn'nnn_
Cuts at: 2409 4400 5462 2409
Size: 1991 1062 6188
Fragments arranged by size:
6188 1991 1062
SbfI CC_TGCA'GG
Cuts at: 4518 6593 4518
Size: 2075 7166
ScaI AGT'ACT
Cuts at: 2352 7489 9201 2352
Size: 5137 1712 2392
Fragments arranged by size:
5137 2392 1712
SexAI A'CCwGG_T
Cuts at: 4599 4599
Size: 9241
SfcI C'TryA_G
Cuts at: 826 1045 2170 2477 2504 3005 4514 4606 5036
Size: 219 1125 307 27 501 1509 92 430
Cuts at: 5036 5211 5630 5717 6112 6589 7724 8405 8596
Size: 175 419 87 395 477 1135 681 191
Cuts at: 8596 826
Size: 1471
Fragments arranged by size:
1509 1471 1135 1125 681 501 477 430
419 395 307 219 191 175 92 87
27
SfoI GGC'GCC
Cuts at: 4915 4915
Size: 9241
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9241
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9241
SmaI CCC'GGG
Cuts at: 1872 5074 6568 1872
Size: 3202 1494 4545
Fragments arranged by size:
4545 3202 1494
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2263 2582 2591 4145 4367 7345
Size: 197 926 320 319 9 1554 222 2978
Cuts at: 7345 8246 8508 820
Size: 901 262 1553
Fragments arranged by size:
2978 1554 1553 926 901 320 319 262
222 197 9
SnaBI TAC'GTA
Cuts at: 493 6538 7032 493
Size: 6045 494 2702
Fragments arranged by size:
6045 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9241
SphI G_CATG'C
Cuts at: 1132 6097 6599 1132
Size: 4965 502 3774
Fragments arranged by size:
4965 3774 502
SspI AAT'ATT
Cuts at: 5 52 2667 7165 5
Size: 47 2615 4498 2081
Fragments arranged by size:
4498 2615 2081 47
StuI AGG'CCT
Cuts at: 1517 5261 6080 1517
Size: 3744 819 4678
Fragments arranged by size:
4678 3744 819
StyI C'CwwG_G
Cuts at: 513 1065 2022 2070 3060 3288 3399 3547 3651
Size: 552 957 48 990 228 111 148 104
Cuts at: 3651 5574 6680 513
Size: 1923 1106 3074
Fragments arranged by size:
3074 1923 1106 990 957 552 228 148
111 104 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2561 4832 6096 6288 7268 7285 7438
Size: 1255 981 2271 1264 192 980 17 153
Cuts at: 7438 7623 8068 325
Size: 185 445 1498
Fragments arranged by size:
2271 1498 1264 1255 981 980 445 192
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2350 5583 7487
Size: 274 80 33 51 1443 373 3233 1904
Cuts at: 7487 9199 96
Size: 1712 138
Fragments arranged by size:
3233 1904 1712 1443 373 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3490 3522 4268 4300 6485 6517 9201 9233 3490
Size: 32 746 32 2185 32 2684 32 3498
Fragments arranged by size:
3498 2684 2185 746 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 6182 1316
Size: 4866 4375
XbaI T'CTAG_A
Cuts at: 6577 6577
Size: 9241
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2135 4876 5929 2135
Size: 2741 1053 5447
Fragments arranged by size:
5447 2741 1053
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9241
XmaI C'CCGG_G
Cuts at: 1870 5072 6566 1870
Size: 3202 1494 4545
Fragments arranged by size:
4545 3202 1494
XmnI GAAnn'nnTTC
Cuts at: 3069 7370 3069
Size: 4301 4940
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7049 276
Size: 53 83 186 936 5515 2468
Fragments arranged by size:
5515 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII
EcoRV FseI FspAI NruI NsiI PacI PspXI PsrI
RsrII SanDI SfiI SrfI SwaI