Restriction map for
pFN21ASDA0969
(Circular) (Six-base) MAPSORT of: pFN21ASDA0969.seq Check: 199 from: 1 to: 7873
pFN21ASDA0969 7873 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5683 278
Size: 53 83 186 936 4147 2468
Fragments arranged by size:
4147 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4635 5166 5216 1937
Size: 2698 531 50 4594
Fragments arranged by size:
4594 2698 531 50
Acc65I G'GTAC_C
Cuts at: 3004 5194 3004
Size: 2190 5683
AclI AA'CG_TT
Cuts at: 1563 6000 6373 1563
Size: 4437 373 3063
Fragments arranged by size:
4437 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2419 2960 3036 5936 7320 7588 1632
Size: 787 541 76 2900 1384 268 1917
Fragments arranged by size:
2900 1917 1384 787 541 268 76
AfeI AGC'GCT
Cuts at: 2165 2261 2165
Size: 96 7777
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7676
AflIII A'CryG_T
Cuts at: 1203 2394 6772 7749 1203
Size: 1191 4378 977 1327
Fragments arranged by size:
4378 1327 1191 977
AgeI A'CCGG_T
Cuts at: 6704 6704
Size: 7873
AhdI GACnn_n'nnGTC
Cuts at: 6602 6602
Size: 7873
AleI CACnn'nnGTG
Cuts at: 1166 4518 1166
Size: 3352 4521
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7873
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4007 4039 4007
Size: 32 7841
AlwNI CAG_nnn'CTG
Cuts at: 3736 5616 7188 3736
Size: 1880 1572 4421
Fragments arranged by size:
4421 1880 1572
ApaI G_GGCC'C
Cuts at: 2792 3187 2792
Size: 395 7478
ApaLI G'TGCA_C
Cuts at: 5930 7086 5930
Size: 1156 6717
ApoI r'AATT_y
Cuts at: 1493 5182 5408 5560 7669 1493
Size: 3689 226 152 2109 1697
Fragments arranged by size:
3689 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6427 160
Size: 872 5395 1606
Fragments arranged by size:
5395 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7873
AvaI C'yCGr_G
Cuts at: 1870 1943 2450 2493 2643 2783 3881 4026 4701
Size: 73 507 43 150 140 1098 145 675
Cuts at: 4701 5198 1870
Size: 497 4545
Fragments arranged by size:
4545 1098 675 507 497 150 145 140
73 43
BamHI G'GATC_C
Cuts at: 2469 3291 3984 5203 5570 2469
Size: 822 693 1219 367 4772
Fragments arranged by size:
4772 1219 822 693 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3004 4512 4921 5138 5194
Size: 325 217 627 1217 1508 409 217 56
Cuts at: 5194 6649 618
Size: 1455 1842
Fragments arranged by size:
1842 1508 1455 1217 627 409 325 217
217 56
BanII G_rGCy'C
Cuts at: 1391 2450 2792 2963 3187 3258 3413 4139 4160
Size: 1059 342 171 224 71 155 726 21
Cuts at: 4160 4197 4410 4743 4776 4844 5192 1391
Size: 37 213 333 33 68 348 4072
Fragments arranged by size:
4072 1059 726 348 342 333 224 213
171 155 71 68 37 33 21
BbeI G_GCGC'C
Cuts at: 5142 5142
Size: 7873
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3024 928
Size: 971 1125 5777
Fragments arranged by size:
5777 1125 971
BbvCI CC'TCA_GC
Cuts at: 4007 4069 4106 4007
Size: 62 37 7774
Fragments arranged by size:
7774 62 37
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2131 2165 6064 6098 1903
Size: 34 194 34 3899 34 3678
Fragments arranged by size:
3899 3678 194 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2330 5765 6975 2330
Size: 3435 1210 3228
Fragments arranged by size:
3435 3228 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7873
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2695 4282 4568
Size: 107 122 71 631 605 1023 1587 286
Cuts at: 4568 4664 4681 6484 136
Size: 96 17 1803 1525
Fragments arranged by size:
1803 1587 1525 1023 631 605 286 122
107 96 71 17
BglII A'GATC_T
Cuts at: 3975 7868 3975
Size: 3893 3980
BlpI GC'TnA_GC
Cuts at: 2406 5290 2406
Size: 2884 4989
Bme1580I G_kGCm'C
Cuts at: 1790 2529 2792 3187 3393 3427 3460 4034 4278
Size: 739 263 395 206 34 33 574 244
Cuts at: 4278 4338 4926 4956 5934 7090 1790
Size: 60 588 30 978 1156 2573
Fragments arranged by size:
2573 1156 978 739 588 574 395 263
244 206 60 34 33 30
BmgBI CAC'GTC
Cuts at: 1318 3994 4756 4951 1318
Size: 2676 762 195 4240
Fragments arranged by size:
4240 2676 762 195
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2907 3085 3447
Size: 416 53 83 399 24 1490 178 362
Cuts at: 3447 4575 5604 6562 442
Size: 1128 1029 958 1753
Fragments arranged by size:
1753 1490 1128 1029 958 416 399 362
178 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7873
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2776 4957 2776
Size: 2181 5692
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2088 2493 3209 3758 4000 4670 4767 4841
Size: 849 405 716 549 242 670 97 74
Cuts at: 4841 5088 6533 1239
Size: 247 1445 2579
Fragments arranged by size:
2579 1445 849 716 670 549 405 247
242 97 74
Bpu10I CC'TnA_GC
Cuts at: 2342 2619 2625 4007 4069 4106 4133 4838 2342
Size: 277 6 1382 62 37 27 705 5377
Fragments arranged by size:
5377 1382 705 277 62 37 27 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5998 6863 7161 5998
Size: 865 298 6710
Fragments arranged by size:
6710 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3898 6536 882
Size: 617 2399 2638 2219
Fragments arranged by size:
2638 2399 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5170 5664 493
Size: 711 3966 494 2702
Fragments arranged by size:
3966 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 3105 5000 7867 3105
Size: 1895 2867 3111
Fragments arranged by size:
3111 2867 1895
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5139 5680 6062 6697
Size: 53 83 186 936 3606 541 382 635
Cuts at: 6697 275
Size: 1451
Fragments arranged by size:
3606 1451 936 635 541 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2719 2749 6306 6704 6978 7125 7536 2719
Size: 30 3557 398 274 147 411 3056
Fragments arranged by size:
3557 3056 411 398 274 147 30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4007 4037 1178
Size: 30 165 30 2604 30 5014
Fragments arranged by size:
5014 2604 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2468 3076 4068 4414 4502 4558 4685 4862
Size: 1282 608 992 346 88 56 127 177
Cuts at: 4862 5091 1186
Size: 229 3968
Fragments arranged by size:
3968 1282 992 608 346 229 177 127
88 56
BseYI C'CCAG_C
Cuts at: 2114 2579 2740 2823 2949 3401 3622 4743 7076
Size: 465 161 83 126 452 221 1121 2333
Cuts at: 7076 7572 2114
Size: 496 2415
Fragments arranged by size:
2415 2333 1121 496 465 452 221 161
126 83
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2384 3695 3887 1163
Size: 573 648 1311 192 5149
Fragments arranged by size:
5149 1311 648 573 192
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3134 5022 5323 6084 7112 1540
Size: 275 186 1133 1888 301 761 1028 2301
Fragments arranged by size:
2301 1888 1133 1028 761 301 275 186
BsiHKAI G_wGCw'C
Cuts at: 2870 2963 3879 4776 5934 6019 7090 2870
Size: 93 916 897 1158 85 1071 3653
Fragments arranged by size:
3653 1158 1071 916 897 93 85
BsmI GAATG_Cn'
Cuts at: 4402 4509 5384 5477 4402
Size: 107 875 93 6798
Fragments arranged by size:
6798 875 107 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2886 2886
Size: 7873
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2450 2529 2792 2870 2963 3187 3258
Size: 399 660 79 263 78 93 224 71
Cuts at: 3258 3393 3413 3427 3460 3879 4034 4139 4160
Size: 135 20 14 33 419 155 105 21
Cuts at: 4160 4197 4278 4338 4410 4743 4776 4844 4926
Size: 37 81 60 72 333 33 68 82
Cuts at: 4926 4956 5192 5934 6019 7090 1391
Size: 30 236 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 660 419 399 333 263
236 224 155 135 105 93 85 82
81 79 78 72 71 68 60 37
33 33 30 21 20 14
BspEI T'CCGG_A
Cuts at: 7536 7536
Size: 7873
BspHI T'CATG_A
Cuts at: 5762 7492 5762
Size: 1730 6143
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5228 844
Size: 4384 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2110 3378 3396 3447 3469 5760 1394
Size: 429 287 1268 18 51 22 2291 3507
Fragments arranged by size:
3507 2291 1268 429 287 51 22 18
BsrDI GCAATG_nn'
Cuts at: 65 1248 3220 4238 4893 5063 6368 6542 65
Size: 1183 1972 1018 655 170 1305 174 1396
Fragments arranged by size:
1972 1396 1305 1183 1018 655 174 170
BsrFI r'CCGG_y
Cuts at: 1700 1810 2856 2907 3154 3666 3957 6517 6704
Size: 110 1046 51 247 512 291 2560 187
Cuts at: 6704 1700
Size: 2869
Fragments arranged by size:
2869 2560 1046 512 291 247 187 110
51
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7873
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7873
BssSI C'ACGA_G
Cuts at: 4628 5933 6945 4628
Size: 1305 1012 5556
Fragments arranged by size:
5556 1305 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7873
BstBI TT'CG_AA
Cuts at: 2007 6677 2007
Size: 4670 3203
BstEII G'GTnAC_C
Cuts at: 1184 4369 1184
Size: 3185 4688
BstXI CCAn_nnnn'nTGG
Cuts at: 2831 2831
Size: 7873
BstYI r'GATC_y
Cuts at: 1972 2469 2502 3291 3975 3984 5203 5570 5955
Size: 497 33 789 684 9 1219 367 385
Cuts at: 5955 5972 7424 7510 7522 7868 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1219 789 684 497 385 367
346 86 33 17 12 9
Bsu36I CC'TnA_GG
Cuts at: 4845 4845
Size: 7873
BtgI C'CryG_G
Cuts at: 513 1065 2478 3843 5132 513
Size: 552 1413 1365 1289 3254
Fragments arranged by size:
3254 1413 1365 1289 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2134 487
Size: 683 56 908 6226
Fragments arranged by size:
6226 908 683 56
BtsI GCAGTG_nn'
Cuts at: 2255 2367 2987 3490 3794 4389 5389 6183 6203
Size: 112 620 503 304 595 1000 794 20
Cuts at: 6203 2255
Size: 3925
Fragments arranged by size:
3925 1000 794 620 595 503 304 112
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7873
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3202 3237 529
Size: 35 2638 35 5165
Fragments arranged by size:
5165 2638 35 35
DraI TTT'AAA
Cuts at: 5177 5177
Size: 7873
DraIII CAC_nnn'GTG
Cuts at: 3388 3455 3388
Size: 67 7806
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6880 809
Size: 507 5564 1802
Fragments arranged by size:
5564 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2659 2858 3131
Size: 54 1425 50 275 18 829 199 273
Cuts at: 3131 4095 4560 4589 5320 6209 7838 8
Size: 964 465 29 731 889 1629 43
Fragments arranged by size:
1629 1425 964 889 829 731 465 275
273 199 54 50 43 29 18
EagI C'GGCC_G
Cuts at: 1537 1812 3131 5320 1537
Size: 275 1319 2189 4090
Fragments arranged by size:
4090 2189 1319 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2225 2294 2307 5803 1353
Size: 872 69 13 3496 3423
Fragments arranged by size:
3496 3423 872 69 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2926 6460 6834 6980 387
Size: 1064 138 1337 3534 374 146 1280
Fragments arranged by size:
3534 1337 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2961 4774 2961
Size: 1813 6060
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2088 2419 2493 2960 3036 3209 3758
Size: 393 456 331 74 467 76 173 549
Cuts at: 3758 4000 4670 4767 4841 5088 5936 6533 7320
Size: 242 670 97 74 247 848 597 787
Cuts at: 7320 7588 1239
Size: 268 1524
Fragments arranged by size:
1524 848 787 670 597 549 467 456
393 331 268 247 242 173 97 76
74 74
EcoNI CCTnn'n_nnAGG
Cuts at: 5032 5032
Size: 7873
EcoO109I rG'GnC_Cy
Cuts at: 2098 2788 3061 3184 3547 3723 4167 2098
Size: 690 273 123 363 176 444 5804
Fragments arranged by size:
5804 690 444 363 273 176 123
EcoRI G'AATT_C
Cuts at: 5182 5560 5182
Size: 378 7495
FspI TGC'GCA
Cuts at: 6379 6379
Size: 7873
HaeII r_GCGC'y
Cuts at: 1678 2167 2263 3202 5142 7020 1678
Size: 489 96 939 1940 1878 2531
Fragments arranged by size:
2531 1940 1878 939 489 96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2487 2519 3326 3358 6514
Size: 32 135 32 915 32 807 32 3156
Cuts at: 6514 6546 6588 6620 1373
Size: 32 42 32 2626
Fragments arranged by size:
3156 2626 915 807 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5217 5463 669
Size: 988 281 3279 246 3079
Fragments arranged by size:
3279 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7873
HpaI GTT'AAC
Cuts at: 5463 5463
Size: 7873
KasI G'GCGC_C
Cuts at: 5138 5138
Size: 7873
KpnI G_GTAC'C
Cuts at: 3008 5198 3008
Size: 2190 5683
MfeI C'AATT_G
Cuts at: 5472 5472
Size: 7873
MluI A'CGCG_T
Cuts at: 7749 7749
Size: 7873
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4110 4212 4668 6987 7171 1305
Size: 2805 102 456 2319 184 2007
Fragments arranged by size:
2805 2319 2007 456 184 102
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6051
Fragments arranged by size:
6051 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2547 3821 4518 5831 6190 6349 518
Size: 648 1381 1274 697 1313 359 159 2042
Fragments arranged by size:
2042 1381 1313 1274 697 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2744 3238 4975 5289 5966 7114 7359 1606
Size: 1138 494 1737 314 677 1148 245 2120
Fragments arranged by size:
2120 1737 1148 1138 677 494 314 245
NaeI GCC'GGC
Cuts at: 2858 3156 2858
Size: 298 7575
NarI GG'CG_CC
Cuts at: 5139 5139
Size: 7873
NcoI C'CATG_G
Cuts at: 513 1065 2478 513
Size: 552 1413 5908
Fragments arranged by size:
5908 1413 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7873
NgoMIV G'CCGG_C
Cuts at: 2856 3154 2856
Size: 298 7575
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7873
NotI GC'GGCC_GC
Cuts at: 5320 5320
Size: 7873
NspI r_CATG'y
Cuts at: 1132 1229 2414 4688 5047 5231 6776 1132
Size: 97 1185 2274 359 184 1545 2229
Fragments arranged by size:
2274 2229 1545 1185 359 184 97
PasI CC'CwG_GG
Cuts at: 1348 3180 1348
Size: 1832 6041
PciI A'CATG_T
Cuts at: 6772 6772
Size: 7873
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2086 2479 2807 2835 3288 3736 1108
Size: 978 393 328 28 453 448 5245
Fragments arranged by size:
5245 978 453 448 393 328 28
PfoI T'CCnGG_A
Cuts at: 3570 3987 3570
Size: 417 7456
PmeI GTTT'AAAC
Cuts at: 5177 5177
Size: 7873
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3130 3162 4007 4039 5942 5974 7455 7487 3130
Size: 32 845 32 1903 32 1481 32 3516
Fragments arranged by size:
3516 1903 1481 845 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2098 3061 3547 3723 4167 2098
Size: 963 486 176 444 5804
Fragments arranged by size:
5804 963 486 444 176
PshAI GACnn'nnGTC
Cuts at: 1613 4581 1613
Size: 2968 4905
PsiI TTA'TAA
Cuts at: 5443 5443
Size: 7873
PspOMI G'GGCC_C
Cuts at: 2788 3183 2788
Size: 395 7478
PspXI vC'TCGA_Gb
Cuts at: 2493 2643 3881 2493
Size: 150 1238 6485
Fragments arranged by size:
6485 1238 150
PstI C_TGCA'G
Cuts at: 830 3372 3801 4554 4980 5225 830
Size: 2542 429 753 426 245 3478
Fragments arranged by size:
3478 2542 753 429 426 245
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7873
PvuII CAG'CTG
Cuts at: 2744 3238 4975 2744
Size: 494 1737 5642
Fragments arranged by size:
5642 1737 494
SacI G_AGCT'C
Cuts at: 2963 4776 2963
Size: 1813 6060
SalI G'TCGA_C
Cuts at: 1936 5215 1936
Size: 3279 4594
SanDI GG'GwC_CC
Cuts at: 3061 3723 4167 3061
Size: 662 444 6767
Fragments arranged by size:
6767 662 444
SapI GCTCTTCn'nnn_
Cuts at: 2225 2225
Size: 7873
SbfI CC_TGCA'GG
Cuts at: 5225 5225
Size: 7873
ScaI AGT'ACT
Cuts at: 3071 3113 6121 7833 3071
Size: 42 3008 1712 3111
Fragments arranged by size:
3111 3008 1712 42
SfcI C'TryA_G
Cuts at: 826 1045 3368 3415 3797 4078 4550 4976 5221
Size: 219 2323 47 382 281 472 426 245
Cuts at: 5221 6356 7037 7228 826
Size: 1135 681 191 1471
Fragments arranged by size:
2323 1471 1135 681 472 426 382 281
245 219 191 47
SfiI GGCCn_nnn'nGGCC
Cuts at: 2695 4568 2695
Size: 1873 6000
SfoI GGC'GCC
Cuts at: 5140 5140
Size: 7873
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7873
SgrAI Cr'CCGG_yG
Cuts at: 1700 2907 1700
Size: 1207 6666
SmaI CCC'GGG
Cuts at: 1872 4028 4703 5200 1872
Size: 2156 675 497 4545
Fragments arranged by size:
4545 2156 675 497
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2493 2643 3881 5977 6878 7140
Size: 197 926 550 150 1238 2096 901 262
Cuts at: 7140 820
Size: 1553
Fragments arranged by size:
2096 1553 1238 926 901 550 262 197
150
SnaBI TAC'GTA
Cuts at: 493 5170 5664 493
Size: 4677 494 2702
Fragments arranged by size:
4677 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7873
SphI G_CATG'C
Cuts at: 1132 2414 4688 5047 5231 1132
Size: 1282 2274 359 184 3774
Fragments arranged by size:
3774 2274 1282 359 184
SspI AAT'ATT
Cuts at: 5 52 5797 5
Size: 47 5745 2081
Fragments arranged by size:
5745 2081 47
StuI AGG'CCT
Cuts at: 1517 2465 2689 5027 1517
Size: 948 224 2338 4363
Fragments arranged by size:
4363 2338 948 224
StyI C'CwwG_G
Cuts at: 513 1065 2205 2478 3543 4197 5312 513
Size: 552 1140 273 1065 654 1115 3074
Fragments arranged by size:
3074 1140 1115 1065 654 552 273
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2593 2974 3415 3466 3953 4515 5036
Size: 1255 1013 381 441 51 487 562 521
Cuts at: 5036 5900 5917 6070 6255 6700 325
Size: 864 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1013 864 562 521 487 445
441 381 185 153 51 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2497 3069 3111
Size: 274 80 33 51 1443 520 572 42
Cuts at: 3111 6119 7831 96
Size: 3008 1712 138
Fragments arranged by size:
3008 1712 1443 572 520 274 138 80
51 42 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4387 4419 7833 7865 4387
Size: 32 3414 32 4395
Fragments arranged by size:
4395 3414 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4790 1316
Size: 3474 4399
XbaI T'CTAG_A
Cuts at: 5209 5209
Size: 7873
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2083 2083
Size: 7873
XhoI C'TCGA_G
Cuts at: 1943 2493 2643 3881 1943
Size: 550 150 1238 5935
Fragments arranged by size:
5935 1238 550 150
XmaI C'CCGG_G
Cuts at: 1870 4026 4701 5198 1870
Size: 2156 675 497 4545
Fragments arranged by size:
4545 2156 675 497
XmnI GAAnn'nnTTC
Cuts at: 3021 6002 3021
Size: 2981 4892
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5681 276
Size: 53 83 186 936 4147 2468
Fragments arranged by size:
4147 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV
FalI FseI FspAI NruI NsiI PacI PmlI PsrI
RsrII SacII SexAI SrfI SwaI