Restriction map for
pFN21ASDA0967
(Circular) (Six-base) MAPSORT of: pFN21ASDA0967.seq Check: 5628 from: 1 to: 9463
pFN21ASDA0967 9463 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2739 2749 3755 3887 2739
Size: 10 1006 132 8315
Fragments arranged by size:
8315 1006 132 10
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3420 7273 278
Size: 53 83 186 936 1884 3853 2468
Fragments arranged by size:
3853 2468 1884 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4611 6806 1937
Size: 2674 2195 4594
Fragments arranged by size:
4594 2674 2195
Acc65I G'GTAC_C
Cuts at: 6784 6784
Size: 9463
AclI AA'CG_TT
Cuts at: 1563 7590 7963 1563
Size: 6027 373 3063
Fragments arranged by size:
6027 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2367 2498 2529 2785 3395 5955 7526 8910
Size: 735 131 31 256 610 2560 1571 1384
Cuts at: 8910 9178 1632
Size: 268 1917
Fragments arranged by size:
2560 1917 1571 1384 735 610 268 256
131 31
AfeI AGC'GCT
Cuts at: 2906 2906
Size: 9463
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9266
AflIII A'CryG_T
Cuts at: 1203 4617 8362 9339 1203
Size: 3414 3745 977 1327
Fragments arranged by size:
3745 3414 1327 977
AgeI A'CCGG_T
Cuts at: 8294 8294
Size: 9463
AhdI GACnn_n'nnGTC
Cuts at: 8192 8192
Size: 9463
AleI CACnn'nnGTG
Cuts at: 1166 2627 1166
Size: 1461 8002
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2909 1184
Size: 1725 7738
AlwNI CAG_nnn'CTG
Cuts at: 2808 3588 3747 4964 5097 6511 7206 8778 2808
Size: 780 159 1217 133 1414 695 1572 3493
Fragments arranged by size:
3493 1572 1414 1217 780 695 159 133
ApaI G_GGCC'C
Cuts at: 2151 3740 4383 2151
Size: 1589 643 7231
Fragments arranged by size:
7231 1589 643
ApaLI G'TGCA_C
Cuts at: 2498 4656 6373 6694 7520 8676 2498
Size: 2158 1717 321 826 1156 3285
Fragments arranged by size:
3285 2158 1717 1156 826 321
ApoI r'AATT_y
Cuts at: 1493 3121 4885 6772 6998 7150 9259 1493
Size: 1628 1764 1887 226 152 2109 1697
Fragments arranged by size:
2109 1887 1764 1697 1628 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8017 160
Size: 872 6985 1606
Fragments arranged by size:
6985 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9463
AvaI C'yCGr_G
Cuts at: 1870 1943 2106 2121 3835 4796 4830 5431 6171
Size: 73 163 15 1714 961 34 601 740
Cuts at: 6171 6788 1870
Size: 617 4545
Fragments arranged by size:
4545 1714 961 740 617 601 163 73
34 15
AvrII C'CTAG_G
Cuts at: 5744 5744
Size: 9463
BamHI G'GATC_C
Cuts at: 5818 6793 7160 5818
Size: 975 367 8121
Fragments arranged by size:
8121 975 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3648 3664 4078 4585 5384
Size: 325 217 627 1861 16 414 507 799
Cuts at: 5384 5710 6784 8239 618
Size: 326 1074 1455 1842
Fragments arranged by size:
1861 1842 1455 1074 799 627 507 414
326 325 217 16
BanII G_rGCy'C
Cuts at: 1391 2151 3740 4222 4383 4641 4797 5154 5166
Size: 760 1589 482 161 258 156 357 12
Cuts at: 5166 6178 6419 6782 1391
Size: 1012 241 363 4072
Fragments arranged by size:
4072 1589 1012 760 482 363 357 258
241 161 156 12
BbeI G_GCGC'C
Cuts at: 3652 3652
Size: 9463
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2355 2486 2853 4040 4486 4830 6090
Size: 971 456 131 367 1187 446 344 1260
Cuts at: 6090 928
Size: 4301
Fragments arranged by size:
4301 1260 1187 971 456 446 367 344
131
BbvCI CC'TCA_GC
Cuts at: 4915 4915
Size: 9463
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2669 2703 4037 4071 6183 6217 7654
Size: 34 732 34 1334 34 2112 34 1437
Cuts at: 7654 7688 1903
Size: 34 3678
Fragments arranged by size:
3678 2112 1437 1334 732 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7355 8565 7355
Size: 1210 8253
BclI T'GATC_A
Cuts at: 1548 2314 6450 1548
Size: 766 4136 4561
Fragments arranged by size:
4561 4136 766
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4468 5102 6163
Size: 107 122 71 631 605 2796 634 1061
Cuts at: 6163 6707 8074 136
Size: 544 1367 1525
Fragments arranged by size:
2796 1525 1367 1061 634 631 605 544
122 107 71
BglII A'GATC_T
Cuts at: 2997 9458 2997
Size: 6461 3002
BlpI GC'TnA_GC
Cuts at: 6880 6880
Size: 9463
Bme1580I G_kGCm'C
Cuts at: 1790 2151 2502 2986 3740 4081 4383 4660 6244
Size: 361 351 484 754 341 302 277 1584
Cuts at: 6244 6377 6698 7524 8680 1790
Size: 133 321 826 1156 2573
Fragments arranged by size:
2573 1584 1156 826 754 484 361 351
341 321 302 277 133
BmgBI CAC'GTC
Cuts at: 1318 2427 4618 1318
Size: 1109 2191 6163
Fragments arranged by size:
6163 2191 1109
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3034 3500 4018
Size: 416 53 83 399 24 1617 466 518
Cuts at: 4018 4257 4401 7194 8152 442
Size: 239 144 2793 958 1753
Fragments arranged by size:
2793 1753 1617 958 518 466 416 399
239 144 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9463
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3625 6250 3625
Size: 2625 6838
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2054 2204 2882 3871 4622 4733 4759 5053
Size: 815 150 678 989 751 111 26 294
Cuts at: 5053 5138 5463 5673 6181 6220 6332 8123 1239
Size: 85 325 210 508 39 112 1791 2579
Fragments arranged by size:
2579 1791 989 815 751 678 508 325
294 210 150 112 111 85 39 26
Bpu10I CC'TnA_GC
Cuts at: 4915 4968 5778 4915
Size: 53 810 8600
Fragments arranged by size:
8600 810 53
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2555 3185 4986 5118 5550 5592 7588 8453 8751
Size: 630 1801 132 432 42 1996 865 298
Cuts at: 8751 2555
Size: 3267
Fragments arranged by size:
3267 1996 1801 865 630 432 298 132
42
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2030 4576 4709 4745 4793 5553 8126
Size: 617 531 2546 133 36 48 760 2573
Cuts at: 8126 882
Size: 2219
Fragments arranged by size:
2573 2546 2219 760 617 531 133 48
36
BsaAI yAC'GTr
Cuts at: 493 1204 6760 7254 493
Size: 711 5556 494 2702
Fragments arranged by size:
5556 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 5191 9457 5191
Size: 4266 5197
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3417 3649 3788 7270
Size: 53 83 186 936 1884 232 139 3482
Cuts at: 7270 7652 8287 275
Size: 382 635 1451
Fragments arranged by size:
3482 1884 1451 936 635 382 232 186
139 83 53
BsaWI w'CCGG_w
Cuts at: 4860 7896 8294 8568 8715 9126 4860
Size: 3036 398 274 147 411 5197
Fragments arranged by size:
5197 3036 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2648 2678 2702 2732 3866
Size: 30 165 30 1245 30 24 30 1134
Cuts at: 3866 3896 4757 4787 5136 5166 1178
Size: 30 861 30 349 30 5475
Fragments arranged by size:
5475 1245 1134 861 349 165 30 30
30 30 30 30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2207 2474 2789 3617 3704 3874 3971 4731
Size: 1021 267 315 828 87 170 97 760
Cuts at: 4731 4903 4917 5417 1186
Size: 172 14 500 5232
Fragments arranged by size:
5232 1021 828 760 500 315 267 172
170 97 87 14
BseYI C'CCAG_C
Cuts at: 2985 3162 3479 3740 4074 4257 4768 8666 9162
Size: 177 317 261 334 183 511 3898 496
Cuts at: 9162 2985
Size: 3286
Fragments arranged by size:
3898 3286 511 496 334 317 261 183
177
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2407 2711 2720 2919 2939 4274 6043
Size: 573 671 304 9 199 20 1335 1769
Cuts at: 6043 6169 1163
Size: 126 4457
Fragments arranged by size:
4457 1769 1335 671 573 304 199 126
20 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6711 6913 7674 8702 1540
Size: 275 186 4710 202 761 1028 2301
Fragments arranged by size:
4710 2301 1028 761 275 202 186
BsiHKAI G_wGCw'C
Cuts at: 2502 2520 2571 2898 4094 4660 4763 5154 6377
Size: 18 51 327 1196 566 103 391 1223
Cuts at: 6377 6698 7524 7609 8680 2502
Size: 321 826 85 1071 3285
Fragments arranged by size:
3285 1223 1196 1071 826 566 391 327
321 103 85 51 18
BsmI GAATG_Cn'
Cuts at: 6974 7067 6974
Size: 93 9370
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2151 2502 2520 2571 2898 2986 3740
Size: 399 361 351 18 51 327 88 754
Cuts at: 3740 4081 4094 4222 4383 4641 4660 4763 4797
Size: 341 13 128 161 258 19 103 34
Cuts at: 4797 5154 5166 6178 6244 6377 6419 6698 6782
Size: 357 12 1012 66 133 42 279 84
Cuts at: 6782 7524 7609 8680 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 1012 754 742 399 361 357
351 341 327 279 258 161 133 128
103 88 85 84 66 51 42 34
19 18 13 12
BspEI T'CCGG_A
Cuts at: 4860 9126 4860
Size: 4266 5197
BspHI T'CATG_A
Cuts at: 7352 9082 7352
Size: 1730 7733
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2739 2749 3755 3882 3887 6653 6818 844
Size: 1895 10 1006 127 5 2766 165 3489
Fragments arranged by size:
3489 2766 1895 1006 165 127 10 5
BsrBI CCG'CTC
Cuts at: 1394 1823 7350 1394
Size: 429 5527 3507
Fragments arranged by size:
5527 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3261 5842 7958 8132 65
Size: 1183 2013 2581 2116 174 1396
Fragments arranged by size:
2581 2116 2013 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6524 8107 8294 1700
Size: 110 4714 1583 187 2869
Fragments arranged by size:
4714 2869 1583 187 110
BsrGI T'GTAC_A
Cuts at: 96 5201 6606 96
Size: 5105 1405 2953
Fragments arranged by size:
5105 2953 1405
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9463
BssSI C'ACGA_G
Cuts at: 7523 8535 7523
Size: 1012 8451
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2734 3344 4690 794
Size: 1940 610 1346 5567
Fragments arranged by size:
5567 1940 1346 610
BstBI TT'CG_AA
Cuts at: 2007 8267 2007
Size: 6260 3203
BstEII G'GTnAC_C
Cuts at: 1184 5075 1184
Size: 3891 5572
BstXI CCAn_nnnn'nTGG
Cuts at: 2228 4726 4783 5968 2228
Size: 2498 57 1185 5723
Fragments arranged by size:
5723 2498 1185 57
BstYI r'GATC_y
Cuts at: 1972 2319 2970 2997 3186 5538 5818 6793 7160
Size: 347 651 27 189 2352 280 975 367
Cuts at: 7160 7545 7562 9014 9100 9112 9458 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
2352 1977 1452 975 651 385 367 347
346 280 189 86 27 17 12
Bsu36I CC'TnA_GG
Cuts at: 3720 4526 6279 6593 3720
Size: 806 1753 314 6590
Fragments arranged by size:
6590 1753 806 314
BtgI C'CryG_G
Cuts at: 513 1065 2022 2853 3814 3846 4803 6055 6108
Size: 552 957 831 961 32 957 1252 53
Cuts at: 6108 6747 513
Size: 639 3229
Fragments arranged by size:
3229 1252 961 957 957 831 639 552
53 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2904 487
Size: 683 56 1678 7046
Fragments arranged by size:
7046 1678 683 56
BtsI GCAGTG_nn'
Cuts at: 3598 6979 7773 7793 3598
Size: 3381 794 20 5268
Fragments arranged by size:
5268 3381 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9463
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9428
DraI TTT'AAA
Cuts at: 3006 3174 6767 3006
Size: 168 3593 5702
Fragments arranged by size:
5702 3593 168
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8470 809
Size: 507 7154 1802
Fragments arranged by size:
7154 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4167 5103 5958
Size: 54 1425 50 275 18 2337 936 855
Cuts at: 5958 6221 6522 6708 6910 7799 9428 8
Size: 263 301 186 202 889 1629 43
Fragments arranged by size:
2337 1629 1425 936 889 855 301 275
263 202 186 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6708 6910 1537
Size: 275 4896 202 4090
Fragments arranged by size:
4896 4090 275 202
EarI CTCTTCn'nnn_
Cuts at: 1353 2022 2459 2697 2736 2804 3104 3369 3855
Size: 669 437 238 39 68 300 265 486
Cuts at: 3855 5322 6468 7393 1353
Size: 1467 1146 925 3423
Fragments arranged by size:
3423 1467 1146 925 669 486 437 300
265 238 68 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6553 8050 8424 8570 387
Size: 1064 138 4964 1497 374 146 1280
Fragments arranged by size:
4964 1497 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5152 5152
Size: 9463
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2204 2367 2498 2529 2785 2882
Size: 393 422 150 163 131 31 256 97
Cuts at: 2882 3395 3871 4622 4733 4759 5053 5138 5463
Size: 513 476 751 111 26 294 85 325
Cuts at: 5463 5673 5955 6181 6220 6332 7526 8123 8910
Size: 210 282 226 39 112 1194 597 787
Cuts at: 8910 9178 1239
Size: 268 1524
Fragments arranged by size:
1524 1194 787 751 597 513 476 422
393 325 294 282 268 256 226 210
163 150 131 112 111 97 85 39
31 26
EcoNI CCTnn'n_nnAGG
Cuts at: 2219 2456 3889 5523 2219
Size: 237 1433 1634 6159
Fragments arranged by size:
6159 1634 1433 237
EcoO109I rG'GnC_Cy
Cuts at: 2824 3585 3736 3737 4367 4379 2824
Size: 761 151 1 630 12 7908
Fragments arranged by size:
7908 761 630 151 12 1
EcoRI G'AATT_C
Cuts at: 4885 6772 7150 4885
Size: 1887 378 7198
Fragments arranged by size:
7198 1887 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2417 2518 2596 3425 4557 2417
Size: 101 78 829 1132 7323
Fragments arranged by size:
7323 1132 829 101 78
FseI GG_CCGG'CC
Cuts at: 6528 6528
Size: 9463
FspI TGC'GCA
Cuts at: 7969 7969
Size: 9463
HaeII r_GCGC'y
Cuts at: 1678 2104 2908 3652 8610 1678
Size: 426 804 744 4958 2531
Fragments arranged by size:
4958 2531 804 744 426
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2648 2680 3239 3271 3866
Size: 32 135 32 1076 32 559 32 595
Cuts at: 3866 3898 4304 4336 4547 4579 5459 5491 5983
Size: 32 406 32 211 32 880 32 492
Cuts at: 5983 6015 8104 8136 8178 8210 1373
Size: 32 2089 32 42 32 2626
Fragments arranged by size:
2626 2089 1076 880 595 559 492 406
211 135 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3502 6807 7053 669
Size: 988 281 1564 3305 246 3079
Fragments arranged by size:
3305 3079 1564 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2300 2601 4417 748
Size: 1552 301 1816 5794
Fragments arranged by size:
5794 1816 1552 301
HpaI GTT'AAC
Cuts at: 7053 7053
Size: 9463
KasI G'GCGC_C
Cuts at: 3648 3648
Size: 9463
KpnI G_GTAC'C
Cuts at: 6788 6788
Size: 9463
MfeI C'AATT_G
Cuts at: 7062 7062
Size: 9463
MluI A'CGCG_T
Cuts at: 9339 9339
Size: 9463
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2965 3074 3256 3471 3816 5139 5157 5613
Size: 1660 109 182 215 345 1323 18 456
Cuts at: 5613 6316 6328 8577 8761 1305
Size: 703 12 2249 184 2007
Fragments arranged by size:
2249 2007 1660 1323 703 456 345 215
184 182 109 18 12
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5105 5960 6223 10
Size: 54 1425 343 3273 855 263 3250
Fragments arranged by size:
3273 3250 1425 855 343 263 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2180 2627 4787 5803 7421 7780 7939
Size: 648 1014 447 2160 1016 1618 359 159
Cuts at: 7939 518
Size: 2042
Fragments arranged by size:
2160 2042 1618 1016 1014 648 447 359
159
MspA1I CmG'CkG
Cuts at: 1606 2141 2793 3816 5483 6027 6183 6253 6520
Size: 535 652 1023 1667 544 156 70 267
Cuts at: 6520 6558 6584 6879 7556 8704 8949 1606
Size: 38 26 295 677 1148 245 2120
Fragments arranged by size:
2120 1667 1148 1023 677 652 544 535
295 267 245 156 70 38 26
NaeI GCC'GGC
Cuts at: 6526 6526
Size: 9463
NarI GG'CG_CC
Cuts at: 3649 3649
Size: 9463
NcoI C'CATG_G
Cuts at: 513 1065 2022 3846 4803 6108 513
Size: 552 957 1824 957 1305 3868
Fragments arranged by size:
3868 1824 1305 957 957 552
NdeI CA'TA_TG
Cuts at: 387 3208 387
Size: 2821 6642
NgoMIV G'CCGG_C
Cuts at: 6524 6524
Size: 9463
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9463
NotI GC'GGCC_GC
Cuts at: 6910 6910
Size: 9463
NspI r_CATG'y
Cuts at: 1132 1229 4693 6051 6449 6821 8366 1132
Size: 97 3464 1358 398 372 1545 2229
Fragments arranged by size:
3464 2229 1545 1358 398 372 97
PasI CC'CwG_GG
Cuts at: 1348 5588 1348
Size: 4240 5223
PciI A'CATG_T
Cuts at: 8362 8362
Size: 9463
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2761 6298 6511 1108
Size: 1653 3537 213 4060
Fragments arranged by size:
4060 3537 1653 213
PfoI T'CCnGG_A
Cuts at: 2220 2898 2961 3996 5293 2220
Size: 678 63 1035 1297 6390
Fragments arranged by size:
6390 1297 1035 678 63
PmeI GTTT'AAAC
Cuts at: 6767 6767
Size: 9463
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4171 4203 7532 7564 9045 9077 4171
Size: 32 3329 32 1481 32 4557
Fragments arranged by size:
4557 3329 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2824 2824
Size: 9463
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9463
PsiI TTA'TAA
Cuts at: 7033 7033
Size: 9463
PspOMI G'GGCC_C
Cuts at: 2147 3736 4379 2147
Size: 1589 643 7231
Fragments arranged by size:
7231 1589 643
PspXI vC'TCGA_Gb
Cuts at: 3835 3835
Size: 9463
PstI C_TGCA'G
Cuts at: 830 2140 2880 2888 3565 3593 3674 4402 4686
Size: 1310 740 8 677 28 81 728 284
Cuts at: 4686 6815 830
Size: 2129 3478
Fragments arranged by size:
3478 2129 1310 740 728 677 284 81
28 8
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9463
PvuII CAG'CTG
Cuts at: 2141 5483 6027 6183 6253 6520 2141
Size: 3342 544 156 70 267 5084
Fragments arranged by size:
5084 3342 544 267 156 70
SacI G_AGCT'C
Cuts at: 5154 5154
Size: 9463
SacII CC_GC'GG
Cuts at: 3817 3817
Size: 9463
SalI G'TCGA_C
Cuts at: 1936 6805 1936
Size: 4869 4594
SapI GCTCTTCn'nnn_
Cuts at: 2022 2697 5322 2022
Size: 675 2625 6163
Fragments arranged by size:
6163 2625 675
SbfI CC_TGCA'GG
Cuts at: 6815 6815
Size: 9463
ScaI AGT'ACT
Cuts at: 2684 6230 7711 9423 2684
Size: 3546 1481 1712 2724
Fragments arranged by size:
3546 2724 1712 1481
SexAI A'CCwGG_T
Cuts at: 6510 6510
Size: 9463
SfcI C'TryA_G
Cuts at: 826 1045 2136 2876 2884 2913 3182 3487 3561
Size: 219 1091 740 8 29 269 305 74
Cuts at: 3561 3589 3670 3820 4141 4398 4547 4682 6550
Size: 28 81 150 321 257 149 135 1868
Cuts at: 6550 6811 7946 8627 8818 826
Size: 261 1135 681 191 1471
Fragments arranged by size:
1868 1471 1135 1091 740 681 321 305
269 261 257 219 191 150 149 135
81 74 29 28 8
SfiI GGCCn_nnn'nGGCC
Cuts at: 6707 6707
Size: 9463
SfoI GGC'GCC
Cuts at: 3650 3650
Size: 9463
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9463
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9463
SmaI CCC'GGG
Cuts at: 1872 2108 2123 5433 6790 1872
Size: 236 15 3310 1357 4545
Fragments arranged by size:
4545 3310 1357 236 15
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2570 3200 3835 4830 5001 5133
Size: 197 926 627 630 635 995 171 132
Cuts at: 5133 5565 5607 7567 8468 8730 820
Size: 432 42 1960 901 262 1553
Fragments arranged by size:
1960 1553 995 926 901 635 630 627
432 262 197 171 132 42
SnaBI TAC'GTA
Cuts at: 493 6760 7254 493
Size: 6267 494 2702
Fragments arranged by size:
6267 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9463
SphI G_CATG'C
Cuts at: 1132 6821 1132
Size: 5689 3774
SrfI GCCC'GGGC
Cuts at: 2123 2123
Size: 9463
SspI AAT'ATT
Cuts at: 5 52 7387 5
Size: 47 7335 2081
Fragments arranged by size:
7335 2081 47
StuI AGG'CCT
Cuts at: 1517 3924 4724 4978 6166 1517
Size: 2407 800 254 1188 4814
Fragments arranged by size:
4814 2407 1188 800 254
StyI C'CwwG_G
Cuts at: 513 1065 2022 2820 3846 4651 4719 4725 4803
Size: 552 957 798 1026 805 68 6 78
Cuts at: 4803 5744 6108 6902 513
Size: 941 364 794 3074
Fragments arranged by size:
3074 1026 957 941 805 798 794 552
364 78 68 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2471 4308 4568 5546 7490 7507 7660
Size: 1255 891 1837 260 978 1944 17 153
Cuts at: 7660 7845 8290 325
Size: 185 445 1498
Fragments arranged by size:
1944 1837 1498 1255 978 891 445 260
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2682 2709 4196
Size: 274 80 33 51 1443 705 27 1487
Cuts at: 4196 5201 6228 6606 7709 9421 96
Size: 1005 1027 378 1103 1712 138
Fragments arranged by size:
1712 1487 1443 1103 1027 1005 705 378
274 138 80 51 33 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5136 5168 5667 5699 6132 6164 9423 9455 5136
Size: 32 499 32 433 32 3259 32 5144
Fragments arranged by size:
5144 3259 499 433 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9463
XbaI T'CTAG_A
Cuts at: 6799 6799
Size: 9463
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2029 2827 3721 3853 4270 5953 6010 2029
Size: 798 894 132 417 1683 57 5482
Fragments arranged by size:
5482 1683 894 798 417 132 57
XhoI C'TCGA_G
Cuts at: 1943 3835 4830 1943
Size: 1892 995 6576
Fragments arranged by size:
6576 1892 995
XmaI C'CCGG_G
Cuts at: 1870 2106 2121 5431 6788 1870
Size: 236 15 3310 1357 4545
Fragments arranged by size:
4545 3310 1357 236 15
XmnI GAAnn'nnTTC
Cuts at: 7592 7592
Size: 9463
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3418 7271 276
Size: 53 83 186 936 1884 3853 2468
Fragments arranged by size:
3853 2468 1884 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BfrBI BsiWI BsmBI BstZ17I DraIII
EcoRV FspAI NruI NsiI PacI PmlI PsrI RsrII
SanDI SwaI