Restriction map for
pFN21ASDA0962
(Circular) (Six-base) MAPSORT of: pFN21ASDA0962.seq Check: 9577 from: 1 to: 7075
pFN21ASDA0962 7075 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4885 278
Size: 53 83 186 936 3349 2468
Fragments arranged by size:
3349 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3288 3957 4418 1937
Size: 1351 669 461 4594
Fragments arranged by size:
4594 1351 669 461
Acc65I G'GTAC_C
Cuts at: 4396 4396
Size: 7075
AclI AA'CG_TT
Cuts at: 1563 5202 5575 1563
Size: 3639 373 3063
Fragments arranged by size:
3639 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3525 5138 6522 6790 1632
Size: 1893 1613 1384 268 1917
Fragments arranged by size:
1917 1893 1613 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6878
AflIII A'CryG_T
Cuts at: 1203 5974 6951 1203
Size: 4771 977 1327
Fragments arranged by size:
4771 1327 977
AgeI A'CCGG_T
Cuts at: 5906 5906
Size: 7075
AhdI GACnn_n'nnGTC
Cuts at: 5804 5804
Size: 7075
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7075
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3327 1184
Size: 2143 4932
AlwNI CAG_nnn'CTG
Cuts at: 4818 6390 4818
Size: 1572 5503
ApaLI G'TGCA_C
Cuts at: 5132 6288 5132
Size: 1156 5919
ApoI r'AATT_y
Cuts at: 1493 2085 2374 3446 3913 3941 4040 4384 4610
Size: 592 289 1072 467 28 99 344 226
Cuts at: 4610 4762 6871 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1072 592 467 344 289 226
152 99 28
AseI AT'TA_AT
Cuts at: 160 1032 5629 160
Size: 872 4597 1606
Fragments arranged by size:
4597 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7075
AvaI C'yCGr_G
Cuts at: 1870 1943 2181 3048 4236 4400 1870
Size: 73 238 867 1188 164 4545
Fragments arranged by size:
4545 1188 867 238 164 73
AvrII C'CTAG_G
Cuts at: 2122 2650 2677 2122
Size: 528 27 6520
Fragments arranged by size:
6520 528 27
BamHI G'GATC_C
Cuts at: 4262 4405 4772 4262
Size: 143 367 6565
Fragments arranged by size:
6565 367 143
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4396 5851 618
Size: 325 217 627 2609 1455 1842
Fragments arranged by size:
2609 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4328 4394 1391
Size: 2937 66 4072
Fragments arranged by size:
4072 2937 66
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2229 2747 3423 3764 928
Size: 971 330 518 676 341 4239
Fragments arranged by size:
4239 971 676 518 341 330
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2533 2567 3518 3552 3667 3701 5266
Size: 34 596 34 951 34 115 34 1565
Cuts at: 5266 5300 1903
Size: 34 3678
Fragments arranged by size:
3678 1565 951 596 115 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4967 6177 4967
Size: 1210 5865
BclI T'GATC_A
Cuts at: 1548 2290 1548
Size: 742 6333
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2147 5686 136
Size: 107 122 71 631 605 475 3539 1525
Fragments arranged by size:
3539 1525 631 605 475 122 107 71
BglII A'GATC_T
Cuts at: 7070 7070
Size: 7075
BlpI GC'TnA_GC
Cuts at: 4492 4492
Size: 7075
Bme1580I G_kGCm'C
Cuts at: 1790 2659 5136 6292 1790
Size: 869 2477 1156 2573
Fragments arranged by size:
2573 2477 1156 869
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7075
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3771 3978 4806
Size: 416 53 83 399 24 2354 207 828
Cuts at: 4806 5764 442
Size: 958 1753
Fragments arranged by size:
2354 1753 958 828 416 399 207 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7075
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2235 2328 3640 3717 4340 5735 1239
Size: 996 93 1312 77 623 1395 2579
Fragments arranged by size:
2579 1395 1312 996 623 93 77
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3246 4032 5200 6065 6363 3246
Size: 786 1168 865 298 3958
Fragments arranged by size:
3958 1168 865 786 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5738 882
Size: 617 4239 2219
Fragments arranged by size:
4239 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4372 4866 493
Size: 711 3168 494 2702
Fragments arranged by size:
3168 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7069 7069
Size: 7075
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2635 4882 5264 5899
Size: 53 83 186 936 1102 2247 382 635
Cuts at: 5899 275
Size: 1451
Fragments arranged by size:
2247 1451 1102 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5508 5906 6180 6327 6738 5508
Size: 398 274 147 411 5845
Fragments arranged by size:
5845 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3424 3454 1178
Size: 30 165 30 2021 30 4799
Fragments arranged by size:
4799 2021 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4193 1186
Size: 3007 4068
BseYI C'CCAG_C
Cuts at: 2621 3594 6278 6774 2621
Size: 973 2684 496 2922
Fragments arranged by size:
2922 2684 973 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4219 1163
Size: 573 2483 4019
Fragments arranged by size:
4019 2483 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3161 4525 5286 6314 1540
Size: 275 186 1160 1364 761 1028 2301
Fragments arranged by size:
2301 1364 1160 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5136 5221 6292 5136
Size: 85 1071 5919
Fragments arranged by size:
5919 1071 85
BsmI GAATG_Cn'
Cuts at: 2544 4586 4679 2544
Size: 2042 93 4940
Fragments arranged by size:
4940 2042 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2295 2626 2295
Size: 331 6744
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2659 4328 4394 5136 5221 6292 1391
Size: 399 869 1669 66 742 85 1071 2174
Fragments arranged by size:
2174 1669 1071 869 742 399 85 66
BspEI T'CCGG_A
Cuts at: 6738 6738
Size: 7075
BspHI T'CATG_A
Cuts at: 4964 6694 4964
Size: 1730 5345
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4430 844
Size: 3586 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4962 1394
Size: 429 3139 3507
Fragments arranged by size:
3507 3139 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5570 5744 65
Size: 1183 4322 174 1396
Fragments arranged by size:
4322 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5719 5906 1700
Size: 110 3909 187 2869
Fragments arranged by size:
3909 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7075
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7075
BssSI C'ACGA_G
Cuts at: 5135 6147 5135
Size: 1012 6063
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7075
BstBI TT'CG_AA
Cuts at: 2007 5879 2007
Size: 3872 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7075
BstXI CCAn_nnnn'nTGG
Cuts at: 3865 3865
Size: 7075
BstYI r'GATC_y
Cuts at: 1972 2209 3484 4262 4405 4772 5157 5174 6626
Size: 237 1275 778 143 367 385 17 1452
Cuts at: 6626 6712 6724 7070 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1275 778 385 367 346 237
143 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5566
Fragments arranged by size:
5566 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6336
Fragments arranged by size:
6336 683 56
BtsI GCAGTG_nn'
Cuts at: 3516 4207 4591 5385 5405 3516
Size: 691 384 794 20 5186
Fragments arranged by size:
5186 794 691 384 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7075
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7040
DraI TTT'AAA
Cuts at: 3010 4379 3010
Size: 1369 5706
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6082 809
Size: 507 4766 1802
Fragments arranged by size:
4766 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4129 4522 5411
Size: 54 1425 50 275 18 2299 393 889
Cuts at: 5411 7040 8
Size: 1629 43
Fragments arranged by size:
2299 1629 1425 889 393 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4522 1537
Size: 275 2710 4090
Fragments arranged by size:
4090 2710 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2949 3498 3668 3677 4157 4170 4219 5005
Size: 1596 549 170 9 480 13 49 786
Cuts at: 5005 1353
Size: 3423
Fragments arranged by size:
3423 1596 786 549 480 170 49 13
9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2348 5662 6036 6182 387
Size: 1064 138 759 3314 374 146 1280
Fragments arranged by size:
3314 1280 1064 759 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2235 2328 3525 3640 3717 4340 5138
Size: 393 603 93 1197 115 77 623 798
Cuts at: 5138 5735 6522 6790 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1197 798 787 623 603 597 393
268 115 93 77
EcoNI CCTnn'n_nnAGG
Cuts at: 3813 4319 4333 3813
Size: 506 14 6555
Fragments arranged by size:
6555 506 14
EcoRI G'AATT_C
Cuts at: 3913 4384 4762 3913
Size: 471 378 6226
Fragments arranged by size:
6226 471 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3530 4159 3530
Size: 629 6446
FspI TGC'GCA
Cuts at: 5581 5581
Size: 7075
HaeII r_GCGC'y
Cuts at: 1678 6222 1678
Size: 4544 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2900 2932 3569 3601 3707
Size: 32 135 32 1328 32 637 32 106
Cuts at: 3707 3739 4196 4228 5716 5748 5790 5822 1373
Size: 32 457 32 1488 32 42 32 2626
Fragments arranged by size:
2626 1488 1328 637 457 135 106 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2889 4210 4419 4665 669
Size: 988 281 951 1321 209 246 3079
Fragments arranged by size:
3079 1321 988 951 281 246 209
HindIII A'AGCT_T
Cuts at: 748 3778 748
Size: 3030 4045
HpaI GTT'AAC
Cuts at: 4665 4665
Size: 7075
KpnI G_GTAC'C
Cuts at: 4400 4400
Size: 7075
MfeI C'AATT_G
Cuts at: 2433 4674 2433
Size: 2241 4834
MluI A'CGCG_T
Cuts at: 6951 6951
Size: 7075
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2079 6189 6373 1305
Size: 774 4110 184 2007
Fragments arranged by size:
4110 2007 774 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4131 10
Size: 54 1425 343 2299 2954
Fragments arranged by size:
2954 2299 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 2367 3891 3893 5033 5392 5551
Size: 648 861 340 1524 2 1140 359 159
Cuts at: 5551 518
Size: 2042
Fragments arranged by size:
2042 1524 1140 861 648 359 340 159
2
MspA1I CmG'CkG
Cuts at: 1606 3359 3475 3978 4491 5168 6316 6561 1606
Size: 1753 116 503 513 677 1148 245 2120
Fragments arranged by size:
2120 1753 1148 677 513 503 245 116
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5566
Fragments arranged by size:
5566 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7075
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7075
NotI GC'GGCC_GC
Cuts at: 4522 4522
Size: 7075
NspI r_CATG'y
Cuts at: 1132 1229 3287 4433 5978 1132
Size: 97 2058 1146 1545 2229
Fragments arranged by size:
2229 2058 1545 1146 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7075
PciI A'CATG_T
Cuts at: 5974 5974
Size: 7075
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7075
PfoI T'CCnGG_A
Cuts at: 2212 2212
Size: 7075
PmeI GTTT'AAAC
Cuts at: 4379 4379
Size: 7075
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5144 5176 6657 6689 5144
Size: 32 1481 32 5530
Fragments arranged by size:
5530 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7075
PsiI TTA'TAA
Cuts at: 2169 4645 2169
Size: 2476 4599
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2306 2338 2306
Size: 32 7043
PstI C_TGCA'G
Cuts at: 830 4427 830
Size: 3597 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7075
PvuII CAG'CTG
Cuts at: 3475 3978 3475
Size: 503 6572
SalI G'TCGA_C
Cuts at: 1936 4417 1936
Size: 2481 4594
SapI GCTCTTCn'nnn_
Cuts at: 3498 3498
Size: 7075
SbfI CC_TGCA'GG
Cuts at: 4427 4427
Size: 7075
ScaI AGT'ACT
Cuts at: 3179 5323 7035 3179
Size: 2144 1712 3219
Fragments arranged by size:
3219 2144 1712
SfcI C'TryA_G
Cuts at: 826 1045 3289 3935 4423 5558 6239 6430 826
Size: 219 2244 646 488 1135 681 191 1471
Fragments arranged by size:
2244 1471 1135 681 646 488 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7075
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7075
SmaI CCC'GGG
Cuts at: 1872 2183 4402 1872
Size: 311 2219 4545
Fragments arranged by size:
4545 2219 311
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3261 4047 4236 5179 6080 6342
Size: 197 926 1318 786 189 943 901 262
Cuts at: 6342 820
Size: 1553
Fragments arranged by size:
1553 1318 943 926 901 786 262 197
189
SnaBI TAC'GTA
Cuts at: 493 4372 4866 493
Size: 3879 494 2702
Fragments arranged by size:
3879 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7075
SphI G_CATG'C
Cuts at: 1132 4433 1132
Size: 3301 3774
SspI AAT'ATT
Cuts at: 5 52 4999 5
Size: 47 4947 2081
Fragments arranged by size:
4947 2081 47
StuI AGG'CCT
Cuts at: 1517 3028 1517
Size: 1511 5564
StyI C'CwwG_G
Cuts at: 513 1065 2022 2122 2650 2677 3422 4258 4514
Size: 552 957 100 528 27 745 836 256
Cuts at: 4514 513
Size: 3074
Fragments arranged by size:
3074 957 836 745 552 528 256 100
27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2585 3542 4217 5102 5119 5272 5457
Size: 1255 1005 957 675 885 17 153 185
Cuts at: 5457 5902 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1005 957 885 675 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3177 4114 5321
Size: 274 80 33 51 1443 1200 937 1207
Cuts at: 5321 7033 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1207 1200 937 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2595 2627 3166 3198 3384 3416 3418 3424 3450
Size: 32 539 32 186 32 2 6 26
Cuts at: 3450 3456 7035 7067 2595
Size: 6 3579 32 2603
Fragments arranged by size:
3579 2603 539 186 32 32 32 32
26 6 6 2
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7075
XbaI T'CTAG_A
Cuts at: 4003 4411 4003
Size: 408 6667
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3758 3758
Size: 7075
XhoI C'TCGA_G
Cuts at: 1943 4236 1943
Size: 2293 4782
XmaI C'CCGG_G
Cuts at: 1870 2181 4400 1870
Size: 311 2219 4545
Fragments arranged by size:
4545 2219 311
XmnI GAAnn'nnTTC
Cuts at: 2481 2574 2754 3131 4142 5204 2481
Size: 93 180 377 1011 1062 4352
Fragments arranged by size:
4352 1062 1011 377 180 93
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4883 276
Size: 53 83 186 936 3349 2468
Fragments arranged by size:
3349 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI