Restriction map for
pFN21ASDA0961
(Circular) (Six-base) MAPSORT of: pFN21ASDA0961.seq Check: 7313 from: 1 to: 6286
pFN21ASDA0961 6286 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4096 278
Size: 53 83 186 936 2560 2468
Fragments arranged by size:
2560 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2061 2629 3629 1937
Size: 124 568 1000 4594
Fragments arranged by size:
4594 1000 568 124
Acc65I G'GTAC_C
Cuts at: 3607 3607
Size: 6286
AclI AA'CG_TT
Cuts at: 1563 4413 4786 1563
Size: 2850 373 3063
Fragments arranged by size:
3063 2850 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4349 5733 6001 1632
Size: 2717 1384 268 1917
Fragments arranged by size:
2717 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6089
AflIII A'CryG_T
Cuts at: 1203 2693 5185 6162 1203
Size: 1490 2492 977 1327
Fragments arranged by size:
2492 1490 1327 977
AgeI A'CCGG_T
Cuts at: 5117 5117
Size: 6286
AhdI GACnn_n'nnGTC
Cuts at: 3111 5015 3111
Size: 1904 4382
AleI CACnn'nnGTG
Cuts at: 1166 2817 3069 1166
Size: 1651 252 4383
Fragments arranged by size:
4383 1651 252
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6286
AlwNI CAG_nnn'CTG
Cuts at: 2893 4029 5601 2893
Size: 1136 1572 3578
Fragments arranged by size:
3578 1572 1136
ApaLI G'TGCA_C
Cuts at: 4343 5499 4343
Size: 1156 5130
ApoI r'AATT_y
Cuts at: 1493 2107 2556 2640 2724 3060 3480 3564 3595
Size: 614 449 84 84 336 420 84 31
Cuts at: 3595 3821 3973 6082 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 614 449 420 336 226 152
84 84 84 31
AseI AT'TA_AT
Cuts at: 160 1032 4840 160
Size: 872 3808 1606
Fragments arranged by size:
3808 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6286
AvaI C'yCGr_G
Cuts at: 1870 1943 3611 1870
Size: 73 1668 4545
Fragments arranged by size:
4545 1668 73
BamHI G'GATC_C
Cuts at: 3616 3983 3616
Size: 367 5919
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3607 5062 618
Size: 325 217 627 1820 1455 1842
Fragments arranged by size:
1842 1820 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2217 3605 1391
Size: 826 1388 4072
Fragments arranged by size:
4072 1388 826
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3110 3362 928
Size: 971 1211 252 3852
Fragments arranged by size:
3852 1211 971 252
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4477 4511 1903
Size: 34 2540 34 3678
Fragments arranged by size:
3678 2540 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4178 5388 4178
Size: 1210 5076
BclI T'GATC_A
Cuts at: 1548 2187 1548
Size: 639 5647
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4897 136
Size: 107 122 71 631 605 3225 1525
Fragments arranged by size:
3225 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2685 6281 2685
Size: 3596 2690
BlpI GC'TnA_GC
Cuts at: 3703 3703
Size: 6286
Bme1580I G_kGCm'C
Cuts at: 1790 2620 4347 5503 1790
Size: 830 1727 1156 2573
Fragments arranged by size:
2573 1727 1156 830
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6286
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4017 4975 442
Size: 416 53 83 399 24 2600 958 1753
Fragments arranged by size:
2600 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3006 4946 1239
Size: 1767 1940 2579
Fragments arranged by size:
2579 1940 1767
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4411 5276 5574 4411
Size: 865 298 5123
Fragments arranged by size:
5123 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4949 882
Size: 617 3450 2219
Fragments arranged by size:
3450 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3583 4077 493
Size: 711 2379 494 2702
Fragments arranged by size:
2702 2379 711 494
BsaBI GATnn'nnATC
Cuts at: 6280 6280
Size: 6286
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4093 4475 5110 275
Size: 53 83 186 936 2560 382 635 1451
Fragments arranged by size:
2560 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4719 5117 5391 5538 5949 4719
Size: 398 274 147 411 5056
Fragments arranged by size:
5056 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2781 2811 3033 3063 1178
Size: 30 165 30 1378 30 222 30 4401
Fragments arranged by size:
4401 1378 222 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6286
BseYI C'CCAG_C
Cuts at: 5489 5985 5489
Size: 496 5790
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5713
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3736 4497 5525 1540
Size: 275 186 1735 761 1028 2301
Fragments arranged by size:
2301 1735 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4347 4432 5503 4347
Size: 85 1071 5130
Fragments arranged by size:
5130 1071 85
BsmI GAATG_Cn'
Cuts at: 2090 3797 3890 2090
Size: 1707 93 4486
Fragments arranged by size:
4486 1707 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2217 2620 3605 4347 4432 5503 1391
Size: 399 427 403 985 742 85 1071 2174
Fragments arranged by size:
2174 1071 985 742 427 403 399 85
BspEI T'CCGG_A
Cuts at: 5949 5949
Size: 6286
BspHI T'CATG_A
Cuts at: 2383 4175 5905 2383
Size: 1792 1730 2764
Fragments arranged by size:
2764 1792 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3641 844
Size: 2797 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4173 1394
Size: 429 2350 3507
Fragments arranged by size:
3507 2350 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2898 4781 4955 65
Size: 1183 1650 1883 174 1396
Fragments arranged by size:
1883 1650 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4930 5117 1700
Size: 110 3120 187 2869
Fragments arranged by size:
3120 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2112 96
Size: 2016 4270
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6286
BssSI C'ACGA_G
Cuts at: 2028 4346 5358 2028
Size: 2318 1012 2956
Fragments arranged by size:
2956 2318 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6286
BstBI TT'CG_AA
Cuts at: 2007 5090 2007
Size: 3083 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6286
BstYI r'GATC_y
Cuts at: 1972 2685 3616 3983 4368 4385 5837 5923 5935
Size: 713 931 367 385 17 1452 86 12
Cuts at: 5935 6281 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 931 713 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4777
Fragments arranged by size:
4777 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5547
Fragments arranged by size:
5547 683 56
BtsI GCAGTG_nn'
Cuts at: 3802 4596 4616 3802
Size: 794 20 5472
Fragments arranged by size:
5472 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6286
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6251
DraI TTT'AAA
Cuts at: 3590 3590
Size: 6286
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5293 809
Size: 507 3977 1802
Fragments arranged by size:
3977 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3733 4622 6251
Size: 54 1425 50 275 18 1903 889 1629
Cuts at: 6251 8
Size: 43
Fragments arranged by size:
1903 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3733 1537
Size: 275 1921 4090
Fragments arranged by size:
4090 1921 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2460 4216 1353
Size: 1107 1756 3423
Fragments arranged by size:
3423 1756 1107
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4873 5247 5393 387
Size: 1064 138 3284 374 146 1280
Fragments arranged by size:
3284 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3006 4349 4946 5733 6001 1239
Size: 393 1374 1343 597 787 268 1524
Fragments arranged by size:
1524 1374 1343 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2556 2640 2724 3060 3480 3564 3595 3973 2556
Size: 84 84 336 420 84 31 378 4869
Fragments arranged by size:
4869 420 378 336 84 84 84 31
FspI TGC'GCA
Cuts at: 4792 4792
Size: 6286
HaeII r_GCGC'y
Cuts at: 1678 5433 1678
Size: 3755 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2249 2281 3097 3129 3457
Size: 32 135 32 677 32 816 32 328
Cuts at: 3457 3489 4927 4959 5001 5033 1373
Size: 32 1438 32 42 32 2626
Fragments arranged by size:
2626 1438 816 677 328 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2630 3630 3876 669
Size: 988 281 692 1000 246 3079
Fragments arranged by size:
3079 1000 988 692 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6286
HpaI GTT'AAC
Cuts at: 3876 3876
Size: 6286
KpnI G_GTAC'C
Cuts at: 3611 3611
Size: 6286
MfeI C'AATT_G
Cuts at: 3885 3885
Size: 6286
MluI A'CGCG_T
Cuts at: 6162 6162
Size: 6286
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5400 5584 1305
Size: 4095 184 2007
Fragments arranged by size:
4095 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4464
Fragments arranged by size:
4464 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2565 2733 2817 3069 3153 3321 3405
Size: 648 1399 168 84 252 84 168 84
Cuts at: 3405 3489 3505 4244 4603 4762 518
Size: 84 16 739 359 159 2042
Fragments arranged by size:
2042 1399 739 648 359 252 168 168
159 84 84 84 84 16
MspA1I CmG'CkG
Cuts at: 1606 3702 4379 5527 5772 1606
Size: 2096 677 1148 245 2120
Fragments arranged by size:
2120 2096 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4777
Fragments arranged by size:
4777 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6286
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6286
NotI GC'GGCC_GC
Cuts at: 3733 3733
Size: 6286
NspI r_CATG'y
Cuts at: 1132 1229 2697 3644 5189 1132
Size: 97 1468 947 1545 2229
Fragments arranged by size:
2229 1545 1468 947 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6286
PciI A'CATG_T
Cuts at: 2693 5185 2693
Size: 2492 3794
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6286
PmeI GTTT'AAAC
Cuts at: 3590 3590
Size: 6286
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2011 2043 4355 4387 5868 5900 2011
Size: 32 2312 32 1481 32 2397
Fragments arranged by size:
2397 2312 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6286
PsiI TTA'TAA
Cuts at: 3856 3856
Size: 6286
PstI C_TGCA'G
Cuts at: 830 3638 830
Size: 2808 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6286
SalI G'TCGA_C
Cuts at: 1936 2628 3628 1936
Size: 692 1000 4594
Fragments arranged by size:
4594 1000 692
SbfI CC_TGCA'GG
Cuts at: 3638 3638
Size: 6286
ScaI AGT'ACT
Cuts at: 4534 6246 4534
Size: 1712 4574
SfcI C'TryA_G
Cuts at: 826 1045 2058 2137 3634 4769 5450 5641 826
Size: 219 1013 79 1497 1135 681 191 1471
Fragments arranged by size:
1497 1471 1135 1013 681 219 191 79
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6286
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6286
SmaI CCC'GGG
Cuts at: 1872 3613 1872
Size: 1741 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4390 5291 5553 820
Size: 197 926 2447 901 262 1553
Fragments arranged by size:
2447 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3583 4077 493
Size: 3090 494 2702
Fragments arranged by size:
3090 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6286
SphI G_CATG'C
Cuts at: 1132 3644 1132
Size: 2512 3774
SspI AAT'ATT
Cuts at: 5 52 4210 5
Size: 47 4158 2081
Fragments arranged by size:
4158 2081 47
StuI AGG'CCT
Cuts at: 1517 2855 2939 2958 3023 3443 1517
Size: 1338 84 19 65 420 4360
Fragments arranged by size:
4360 1338 420 84 65 19
StyI C'CwwG_G
Cuts at: 513 1065 2022 3725 513
Size: 552 957 1703 3074
Fragments arranged by size:
3074 1703 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4313 4330 4483 4668 5113 325
Size: 1255 2733 17 153 185 445 1498
Fragments arranged by size:
2733 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2112 2713 2951
Size: 274 80 33 51 1443 135 601 238
Cuts at: 2951 4532 6244 96
Size: 1581 1712 138
Fragments arranged by size:
1712 1581 1443 601 274 238 138 135
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2440 2472 6246 6278 2440
Size: 32 3774 32 2448
Fragments arranged by size:
3774 2448 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6286
XbaI T'CTAG_A
Cuts at: 2254 3622 2254
Size: 1368 4918
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6286
XmaI C'CCGG_G
Cuts at: 1870 3611 1870
Size: 1741 4545
XmnI GAAnn'nnTTC
Cuts at: 3107 3484 4415 3107
Size: 377 931 4978
Fragments arranged by size:
4978 931 377
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4094 276
Size: 53 83 186 936 2560 2468
Fragments arranged by size:
2560 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI