Restriction map for
pFN21ASDA0958
(Circular) (Six-base) MAPSORT of: pFN21ASDA0958.seq Check: 6181 from: 1 to: 6001
pFN21ASDA0958 6001 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2766 2766
Size: 6001
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2172 3811 278
Size: 53 83 186 936 636 1639 2468
Fragments arranged by size:
2468 1639 936 636 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3294 3344 1937
Size: 1357 50 4594
Fragments arranged by size:
4594 1357 50
Acc65I G'GTAC_C
Cuts at: 3322 3322
Size: 6001
AclI AA'CG_TT
Cuts at: 1563 4128 4501 1563
Size: 2565 373 3063
Fragments arranged by size:
3063 2565 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2252 2264 4064 5448 5716 1632
Size: 620 12 1800 1384 268 1917
Fragments arranged by size:
1917 1800 1384 620 268 12
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5804
AflIII A'CryG_T
Cuts at: 1203 4900 5877 1203
Size: 3697 977 1327
Fragments arranged by size:
3697 1327 977
AgeI A'CCGG_T
Cuts at: 4832 4832
Size: 6001
AhdI GACnn_n'nnGTC
Cuts at: 2580 4730 2580
Size: 2150 3851
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6001
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6001
AlwNI CAG_nnn'CTG
Cuts at: 2408 2518 3744 5316 2408
Size: 110 1226 1572 3093
Fragments arranged by size:
3093 1572 1226 110
ApaI G_GGCC'C
Cuts at: 2418 2882 2418
Size: 464 5537
ApaLI G'TGCA_C
Cuts at: 4058 5214 4058
Size: 1156 4845
ApoI r'AATT_y
Cuts at: 1493 3310 3536 3688 5797 1493
Size: 1817 226 152 2109 1697
Fragments arranged by size:
2109 1817 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4555 160
Size: 872 3523 1606
Fragments arranged by size:
3523 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6001
AvaI C'yCGr_G
Cuts at: 1870 1943 3062 3326 1870
Size: 73 1119 264 4545
Fragments arranged by size:
4545 1119 264 73
BamHI G'GATC_C
Cuts at: 2820 3331 3698 2820
Size: 511 367 5123
Fragments arranged by size:
5123 511 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2629 2754 3159 3322 4777
Size: 325 217 627 842 125 405 163 1455
Cuts at: 4777 618
Size: 1842
Fragments arranged by size:
1842 1455 842 627 405 325 217 163
125
BanII G_rGCy'C
Cuts at: 1391 2418 2615 2867 2882 3105 3320 1391
Size: 1027 197 252 15 223 215 4072
Fragments arranged by size:
4072 1027 252 223 215 197 15
BbeI G_GCGC'C
Cuts at: 2633 2633
Size: 6001
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2035 2240 2252 2990 928
Size: 971 136 205 12 738 3939
Fragments arranged by size:
3939 971 738 205 136 12
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4192 4226 1903
Size: 34 2255 34 3678
Fragments arranged by size:
3678 2255 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2719 3893 5103 2719
Size: 1174 1210 3617
Fragments arranged by size:
3617 1210 1174
BclI T'GATC_A
Cuts at: 1548 2509 1548
Size: 961 5040
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4612 136
Size: 107 122 71 631 605 2940 1525
Fragments arranged by size:
2940 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5996 5996
Size: 6001
BlpI GC'TnA_GC
Cuts at: 2614 3418 2614
Size: 804 5197
Bme1580I G_kGCm'C
Cuts at: 1790 2418 2882 4062 5218 1790
Size: 628 464 1180 1156 2573
Fragments arranged by size:
2573 1180 1156 628 464
BmgBI CAC'GTC
Cuts at: 1318 3171 1318
Size: 1853 4148
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2731 2937 3732
Size: 416 53 83 399 24 1314 206 795
Cuts at: 3732 4690 442
Size: 958 1753
Fragments arranged by size:
1753 1314 958 795 416 399 206 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6001
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3119 4661 1239
Size: 1880 1542 2579
Fragments arranged by size:
2579 1880 1542
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4126 4991 5289 4126
Size: 865 298 4838
Fragments arranged by size:
4838 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2567 4664 882
Size: 617 1068 2097 2219
Fragments arranged by size:
2219 2097 1068 617
BsaAI yAC'GTr
Cuts at: 493 1204 3087 3298 3792 493
Size: 711 1883 211 494 2702
Fragments arranged by size:
2702 1883 711 494 211
BsaBI GATnn'nnATC
Cuts at: 5995 5995
Size: 6001
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2169 2630 3808 4190
Size: 53 83 186 936 636 461 1178 382
Cuts at: 4190 4825 275
Size: 635 1451
Fragments arranged by size:
1451 1178 936 636 635 461 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2322 4434 4832 5106 5253 5664 2322
Size: 2112 398 274 147 411 2659
Fragments arranged by size:
2659 2112 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5776
Fragments arranged by size:
5776 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2267 2585 3107 3278 1186
Size: 1081 318 522 171 3909
Fragments arranged by size:
3909 1081 522 318 171
BseYI C'CCAG_C
Cuts at: 2053 2860 5204 5700 2053
Size: 807 2344 496 2354
Fragments arranged by size:
2354 2344 807 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5428
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2121 2501 3183 3451 4212 5240
Size: 275 186 120 380 682 268 761 1028
Cuts at: 5240 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 682 380 275 268 186
120
BsiHKAI G_wGCw'C
Cuts at: 3105 4062 4147 5218 3105
Size: 957 85 1071 3888
Fragments arranged by size:
3888 1071 957 85
BsmI GAATG_Cn'
Cuts at: 3512 3605 3512
Size: 93 5908
BsmBI CGTCTCn'nnnn_
Cuts at: 2596 2766 2596
Size: 170 5831
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2418 2615 2867 2882 3105 3320 4062
Size: 399 628 197 252 15 223 215 742
Cuts at: 4062 4147 5218 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 628 399 252 223 215
197 85 15
BspEI T'CCGG_A
Cuts at: 2322 5664 2322
Size: 3342 2659
BspHI T'CATG_A
Cuts at: 2979 3890 5620 2979
Size: 911 1730 3360
Fragments arranged by size:
3360 1730 911
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2202 2766 3356 844
Size: 1358 564 590 3489
Fragments arranged by size:
3489 1358 590 564
BsrBI CCG'CTC
Cuts at: 1394 1823 2497 3189 3888 1394
Size: 429 674 692 699 3507
Fragments arranged by size:
3507 699 692 674 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4496 4670 65
Size: 1183 3248 174 1396
Fragments arranged by size:
3248 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2991 4645 4832 1700
Size: 110 1181 1654 187 2869
Fragments arranged by size:
2869 1654 1181 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6001
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6001
BssSI C'ACGA_G
Cuts at: 2537 4061 5073 2537
Size: 1524 1012 3465
Fragments arranged by size:
3465 1524 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6001
BstBI TT'CG_AA
Cuts at: 2007 4805 2007
Size: 2798 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6001
BstYI r'GATC_y
Cuts at: 1972 2820 2967 3141 3331 3698 4083 4100 5552
Size: 848 147 174 190 367 385 17 1452
Cuts at: 5552 5638 5650 5996 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 848 385 367 346 190 174
147 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2276 2802 513
Size: 552 957 254 526 3712
Fragments arranged by size:
3712 957 552 526 254
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2383 487
Size: 683 56 1157 4105
Fragments arranged by size:
4105 1157 683 56
BtsI GCAGTG_nn'
Cuts at: 2067 3517 4311 4331 2067
Size: 1450 794 20 3737
Fragments arranged by size:
3737 1450 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6001
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5966
DraI TTT'AAA
Cuts at: 3305 3305
Size: 6001
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5008 809
Size: 507 3692 1802
Fragments arranged by size:
3692 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2089 2118 2498
Size: 54 1425 50 275 18 259 29 380
Cuts at: 2498 3012 3180 3448 4337 5966 8
Size: 514 168 268 889 1629 43
Fragments arranged by size:
1629 1425 889 514 380 275 268 259
168 54 50 43 29 18
EagI C'GGCC_G
Cuts at: 1537 1812 2118 2498 3180 3448 1537
Size: 275 306 380 682 268 4090
Fragments arranged by size:
4090 682 380 306 275 268
EarI CTCTTCn'nnn_
Cuts at: 1353 2475 3036 3931 1353
Size: 1122 561 895 3423
Fragments arranged by size:
3423 1122 895 561
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4588 4962 5108 387
Size: 1064 138 2999 374 146 1280
Fragments arranged by size:
2999 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3103 3103
Size: 6001
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2252 2264 3119 4064 4661 5448 5716
Size: 393 620 12 855 945 597 787 268
Cuts at: 5716 1239
Size: 1524
Fragments arranged by size:
1524 945 855 787 620 597 393 268
12
EcoNI CCTnn'n_nnAGG
Cuts at: 2762 2762
Size: 6001
EcoO109I rG'GnC_Cy
Cuts at: 2415 2472 2878 2879 2415
Size: 57 406 1 5537
Fragments arranged by size:
5537 406 57 1
EcoRI G'AATT_C
Cuts at: 3310 3688 3310
Size: 378 5623
FspI TGC'GCA
Cuts at: 2198 3148 4507 2198
Size: 950 1359 3692
Fragments arranged by size:
3692 1359 950
HaeII r_GCGC'y
Cuts at: 1678 2633 5148 1678
Size: 955 2515 2531
Fragments arranged by size:
2531 2515 955
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2114 2146 2566 2598 2776
Size: 32 135 32 542 32 420 32 178
Cuts at: 2776 2808 3176 3208 4642 4674 4716 4748 1373
Size: 32 368 32 1434 32 42 32 2626
Fragments arranged by size:
2626 1434 542 420 368 178 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2173 3345 3591 669
Size: 988 281 235 1172 246 3079
Fragments arranged by size:
3079 1172 988 281 246 235
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6001
HpaI GTT'AAC
Cuts at: 3591 3591
Size: 6001
KasI G'GCGC_C
Cuts at: 2629 2629
Size: 6001
KpnI G_GTAC'C
Cuts at: 3326 3326
Size: 6001
MfeI C'AATT_G
Cuts at: 3600 3600
Size: 6001
MluI A'CGCG_T
Cuts at: 5877 5877
Size: 6001
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5115 5299 1305
Size: 3810 184 2007
Fragments arranged by size:
3810 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3014 10
Size: 54 1425 343 1182 2997
Fragments arranged by size:
2997 1425 1182 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3959 4318 4477 518
Size: 648 2793 359 159 2042
Fragments arranged by size:
2793 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3417 4094 5242 5487 1606
Size: 1811 677 1148 245 2120
Fragments arranged by size:
2120 1811 1148 677 245
NarI GG'CG_CC
Cuts at: 2630 2630
Size: 6001
NcoI C'CATG_G
Cuts at: 513 1065 2022 2276 513
Size: 552 957 254 4238
Fragments arranged by size:
4238 957 552 254
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6001
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6001
NotI GC'GGCC_GC
Cuts at: 3448 3448
Size: 6001
NspI r_CATG'y
Cuts at: 1132 1229 3359 4904 1132
Size: 97 2130 1545 2229
Fragments arranged by size:
2229 2130 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6001
PciI A'CATG_T
Cuts at: 4900 4900
Size: 6001
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2995 1108
Size: 1887 4114
PfoI T'CCnGG_A
Cuts at: 2829 2829
Size: 6001
PmeI GTTT'AAAC
Cuts at: 3305 3305
Size: 6001
PmlI CAC'GTG
Cuts at: 3087 3087
Size: 6001
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4070 4102 5583 5615 4070
Size: 32 1481 32 4456
Fragments arranged by size:
4456 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2472 2472
Size: 6001
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6001
PsiI TTA'TAA
Cuts at: 3571 3571
Size: 6001
PspOMI G'GGCC_C
Cuts at: 2414 2878 2414
Size: 464 5537
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2378 2410 2378
Size: 32 5969
PstI C_TGCA'G
Cuts at: 830 3353 830
Size: 2523 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6001
RsrII CG'GwC_CG
Cuts at: 2661 2661
Size: 6001
SacI G_AGCT'C
Cuts at: 3105 3105
Size: 6001
SalI G'TCGA_C
Cuts at: 1936 3343 1936
Size: 1407 4594
SapI GCTCTTCn'nnn_
Cuts at: 3036 3036
Size: 6001
SbfI CC_TGCA'GG
Cuts at: 3353 3353
Size: 6001
ScaI AGT'ACT
Cuts at: 2543 3227 4249 5961 2543
Size: 684 1022 1712 2583
Fragments arranged by size:
2583 1712 1022 684
SexAI A'CCwGG_T
Cuts at: 2316 2427 2316
Size: 111 5890
SfcI C'TryA_G
Cuts at: 826 1045 2545 3349 4484 5165 5356 826
Size: 219 1500 804 1135 681 191 1471
Fragments arranged by size:
1500 1471 1135 804 681 219 191
SfoI GGC'GCC
Cuts at: 2631 2631
Size: 6001
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6001
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6001
SmaI CCC'GGG
Cuts at: 1872 3328 1872
Size: 1456 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4105 5006 5268 820
Size: 197 926 2162 901 262 1553
Fragments arranged by size:
2162 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3298 3792 493
Size: 2805 494 2702
Fragments arranged by size:
2805 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6001
SphI G_CATG'C
Cuts at: 1132 3359 1132
Size: 2227 3774
SspI AAT'ATT
Cuts at: 5 52 3925 5
Size: 47 3873 2081
Fragments arranged by size:
3873 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6001
StyI C'CwwG_G
Cuts at: 513 1065 2022 2276 3440 513
Size: 552 957 254 1164 3074
Fragments arranged by size:
3074 1164 957 552 254
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2248 4028 4045 4198 4383 4828 325
Size: 1255 668 1780 17 153 185 445 1498
Fragments arranged by size:
1780 1498 1255 668 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2520 2541 3225
Size: 274 80 33 51 1443 543 21 684
Cuts at: 3225 4247 5959 96
Size: 1022 1712 138
Fragments arranged by size:
1712 1443 1022 684 543 274 138 80
51 33 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5961 5993 5961
Size: 32 5969
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6001
XbaI T'CTAG_A
Cuts at: 3337 3337
Size: 6001
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6001
XmaI C'CCGG_G
Cuts at: 1870 3326 1870
Size: 1456 4545
XmnI GAAnn'nnTTC
Cuts at: 4130 4130
Size: 6001
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2170 3809 276
Size: 53 83 186 936 636 1639 2468
Fragments arranged by size:
2468 1639 936 636 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI
PvuII SacII SanDI SfiI SrfI SwaI XcmI