Restriction map for
pFN21ASDA0957
(Circular) (Six-base) MAPSORT of: pFN21ASDA0957.seq Check: 1563 from: 1 to: 6805
pFN21ASDA0957 6805 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4615 278
Size: 53 83 186 936 3079 2468
Fragments arranged by size:
3079 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4148 1937
Size: 2211 4594
Acc65I G'GTAC_C
Cuts at: 4126 4126
Size: 6805
AclI AA'CG_TT
Cuts at: 1563 4932 5305 1563
Size: 3369 373 3063
Fragments arranged by size:
3369 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2228 4868 6252 6520 1632
Size: 596 2640 1384 268 1917
Fragments arranged by size:
2640 1917 1384 596 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6608
AflIII A'CryG_T
Cuts at: 1203 5704 6681 1203
Size: 4501 977 1327
Fragments arranged by size:
4501 1327 977
AgeI A'CCGG_T
Cuts at: 5636 5636
Size: 6805
AhdI GACnn_n'nnGTC
Cuts at: 3555 5534 3555
Size: 1979 4826
AleI CACnn'nnGTG
Cuts at: 1166 2273 1166
Size: 1107 5698
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2184 2580 1184
Size: 1000 396 5409
Fragments arranged by size:
5409 1000 396
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2465 2497 2465
Size: 32 6773
AlwNI CAG_nnn'CTG
Cuts at: 3454 3649 3840 4548 6120 3454
Size: 195 191 708 1572 4139
Fragments arranged by size:
4139 1572 708 195 191
ApaI G_GGCC'C
Cuts at: 3753 3753
Size: 6805
ApaLI G'TGCA_C
Cuts at: 4862 6018 4862
Size: 1156 5649
ApoI r'AATT_y
Cuts at: 1493 4114 4340 4492 6601 1493
Size: 2621 226 152 2109 1697
Fragments arranged by size:
2621 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5359 160
Size: 872 4327 1606
Fragments arranged by size:
4327 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6805
AvaI C'yCGr_G
Cuts at: 1870 1943 4130 1870
Size: 73 2187 4545
Fragments arranged by size:
4545 2187 73
BamHI G'GATC_C
Cuts at: 4135 4502 4135
Size: 367 6438
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2120 3136 4126 5581 618
Size: 325 217 627 333 1016 990 1455 1842
Fragments arranged by size:
1842 1455 1016 990 627 333 325 217
BanII G_rGCy'C
Cuts at: 1391 3753 4124 1391
Size: 2362 371 4072
Fragments arranged by size:
4072 2362 371
BbeI G_GCGC'C
Cuts at: 2124 2124
Size: 6805
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3648 928
Size: 971 1749 4085
Fragments arranged by size:
4085 1749 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2300 2334 4996 5030 1903
Size: 34 363 34 2662 34 3678
Fragments arranged by size:
3678 2662 363 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4697 5907 4697
Size: 1210 5595
BclI T'GATC_A
Cuts at: 1548 3736 1548
Size: 2188 4617
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3805 5416 136
Size: 107 122 71 631 605 2133 1611 1525
Fragments arranged by size:
2133 1611 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2199 3731 6800 2199
Size: 1532 3069 2204
Fragments arranged by size:
3069 2204 1532
BlpI GC'TnA_GC
Cuts at: 4222 4222
Size: 6805
Bme1580I G_kGCm'C
Cuts at: 1790 2323 3753 3817 4866 6022 1790
Size: 533 1430 64 1049 1156 2573
Fragments arranged by size:
2573 1430 1156 1049 533 64
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6805
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3916 4536 5494
Size: 416 53 83 399 24 2499 620 958
Cuts at: 5494 442
Size: 1753
Fragments arranged by size:
2499 1753 958 620 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6805
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2661 2774 3200 3476 3492 3570 3623 3932
Size: 1422 113 426 276 16 78 53 309
Cuts at: 3932 5465 1239
Size: 1533 2579
Fragments arranged by size:
2579 1533 1422 426 309 276 113 78
53 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4930 5795 6093 4930
Size: 865 298 5642
Fragments arranged by size:
5642 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5468 882
Size: 617 3969 2219
Fragments arranged by size:
3969 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4102 4596 493
Size: 711 2898 494 2702
Fragments arranged by size:
2898 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 6799 6799
Size: 6805
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2121 4612 4994 5629
Size: 53 83 186 936 588 2491 382 635
Cuts at: 5629 275
Size: 1451
Fragments arranged by size:
2491 1451 936 635 588 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5238 5636 5910 6057 6468 5238
Size: 398 274 147 411 5575
Fragments arranged by size:
5575 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2406 2436 2465 2495 2791
Size: 30 165 30 1003 30 29 30 296
Cuts at: 2791 2821 3173 3203 3638 3668 1178
Size: 30 352 30 435 30 4315
Fragments arranged by size:
4315 1003 435 352 296 165 30 30
30 30 30 30 30 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2588 2591 2944 3221 1186
Size: 1402 3 353 277 4770
Fragments arranged by size:
4770 1402 353 277 3
BseYI C'CCAG_C
Cuts at: 2840 3223 3256 3279 3746 3816 4011 6008 6504
Size: 383 33 23 467 70 195 1997 496
Cuts at: 6504 2840
Size: 3141
Fragments arranged by size:
3141 1997 496 467 383 195 70 33
23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2031 2441 4106 1163
Size: 573 295 410 1665 3862
Fragments arranged by size:
3862 1665 573 410 295
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2043 4255 5016 6044 1540
Size: 275 186 42 2212 761 1028 2301
Fragments arranged by size:
2301 2212 1028 761 275 186 42
BsiHKAI G_wGCw'C
Cuts at: 2492 4866 4951 6022 2492
Size: 2374 85 1071 3275
Fragments arranged by size:
3275 2374 1071 85
BsmI GAATG_Cn'
Cuts at: 4316 4409 4316
Size: 93 6712
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2323 2492 3753 3817 4124 4866 4951
Size: 399 533 169 1261 64 307 742 85
Cuts at: 4951 6022 1391
Size: 1071 2174
Fragments arranged by size:
2174 1261 1071 742 533 399 307 169
85 64
BspEI T'CCGG_A
Cuts at: 6468 6468
Size: 6805
BspHI T'CATG_A
Cuts at: 4694 6424 4694
Size: 1730 5075
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3018 3545 4160 844
Size: 2174 527 615 3489
Fragments arranged by size:
3489 2174 615 527
BsrBI CCG'CTC
Cuts at: 1394 1823 4692 1394
Size: 429 2869 3507
Fragments arranged by size:
3507 2869 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5300 5474 65
Size: 1183 4052 174 1396
Fragments arranged by size:
4052 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5449 5636 1700
Size: 110 3639 187 2869
Fragments arranged by size:
3639 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3665 96
Size: 3569 3236
BssHII G'CGCG_C
Cuts at: 1925 2567 1925
Size: 642 6163
BssSI C'ACGA_G
Cuts at: 4865 5877 4865
Size: 1012 5793
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3289 794
Size: 2495 4310
BstBI TT'CG_AA
Cuts at: 2007 5609 2007
Size: 3602 3203
BstEII G'GTnAC_C
Cuts at: 1184 2134 1184
Size: 950 5855
BstXI CCAn_nnnn'nTGG
Cuts at: 2240 2275 2993 2240
Size: 35 718 6052
Fragments arranged by size:
6052 718 35
BstYI r'GATC_y
Cuts at: 1972 2199 3731 4135 4502 4887 4904 6356 6442
Size: 227 1532 404 367 385 17 1452 86
Cuts at: 6442 6454 6800 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1532 1452 404 385 367 346 227
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6253
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2278 487
Size: 683 56 1052 5014
Fragments arranged by size:
5014 1052 683 56
BtsI GCAGTG_nn'
Cuts at: 2604 2824 2851 3621 4321 5115 5135 2604
Size: 220 27 770 700 794 20 4274
Fragments arranged by size:
4274 794 770 700 220 27 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6805
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6770
DraI TTT'AAA
Cuts at: 4109 4109
Size: 6805
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2228 5812 809
Size: 507 912 3584 1802
Fragments arranged by size:
3584 1802 912 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2146 2508 2688
Size: 54 1425 50 275 18 316 362 180
Cuts at: 2688 2859 4252 5141 6770 8
Size: 171 1393 889 1629 43
Fragments arranged by size:
1629 1425 1393 889 362 316 275 180
171 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4252 1537
Size: 275 2440 4090
Fragments arranged by size:
4090 2440 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2796 2877 3785 4050 4735 1353
Size: 1443 81 908 265 685 3423
Fragments arranged by size:
3423 1443 908 685 265 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5392 5766 5912 387
Size: 1064 138 3803 374 146 1280
Fragments arranged by size:
3803 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2228 2661 2774 3200 3476 3492 3570
Size: 393 596 433 113 426 276 16 78
Cuts at: 3570 3623 3932 4868 5465 6252 6520 1239
Size: 53 309 936 597 787 268 1524
Fragments arranged by size:
1524 936 787 597 596 433 426 393
309 276 268 113 78 53 16
EcoO109I rG'GnC_Cy
Cuts at: 2514 3483 3610 3750 2514
Size: 969 127 140 5569
Fragments arranged by size:
5569 969 140 127
EcoRI G'AATT_C
Cuts at: 4114 4492 4114
Size: 378 6427
EcoRV GAT'ATC
Cuts at: 3405 3405
Size: 6805
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2807 4079 2807
Size: 1272 5533
FspI TGC'GCA
Cuts at: 5311 5311
Size: 6805
HaeII r_GCGC'y
Cuts at: 1678 2062 2124 2303 5952 1678
Size: 384 62 179 3649 2531
Fragments arranged by size:
3649 2531 384 179 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2876 2908 3212 3244 3436
Size: 32 135 32 1304 32 304 32 192
Cuts at: 3436 3468 3541 3573 3638 3670 3868 3900 5446
Size: 32 73 32 65 32 198 32 1546
Cuts at: 5446 5478 5520 5552 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1546 1304 304 198 192 135 73
65 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2731 3760 4149 4395 669
Size: 988 281 793 1029 389 246 3079
Fragments arranged by size:
3079 1029 988 793 389 281 246
HindIII A'AGCT_T
Cuts at: 748 3464 748
Size: 2716 4089
HpaI GTT'AAC
Cuts at: 2731 4395 2731
Size: 1664 5141
KasI G'GCGC_C
Cuts at: 2120 2120
Size: 6805
KpnI G_GTAC'C
Cuts at: 4130 4130
Size: 6805
MfeI C'AATT_G
Cuts at: 4404 4404
Size: 6805
MluI A'CGCG_T
Cuts at: 6681 6681
Size: 6805
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3171 3597 5919 6103 1305
Size: 1866 426 2322 184 2007
Fragments arranged by size:
2322 2007 1866 426 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2690 2861 10
Size: 54 1425 343 858 171 3954
Fragments arranged by size:
3954 1425 858 343 171 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2273 4763 5122 5281 518
Size: 648 1107 2490 359 159 2042
Fragments arranged by size:
2490 2042 1107 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2752 4221 4898 6046 6291 1606
Size: 1146 1469 677 1148 245 2120
Fragments arranged by size:
2120 1469 1148 1146 677 245
NarI GG'CG_CC
Cuts at: 2121 2121
Size: 6805
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6253
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6805
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6805
NotI GC'GGCC_GC
Cuts at: 4252 4252
Size: 6805
NspI r_CATG'y
Cuts at: 1132 1229 3017 3439 4163 5708 1132
Size: 97 1788 422 724 1545 2229
Fragments arranged by size:
2229 1788 1545 724 422 97
PasI CC'CwG_GG
Cuts at: 1348 2812 1348
Size: 1464 5341
PciI A'CATG_T
Cuts at: 5704 5704
Size: 6805
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3146 3544 3910 1108
Size: 2038 398 366 4003
Fragments arranged by size:
4003 2038 398 366
PfoI T'CCnGG_A
Cuts at: 2232 2232
Size: 6805
PmeI GTTT'AAAC
Cuts at: 4109 4109
Size: 6805
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2465 2497 4874 4906 6387 6419 2465
Size: 32 2377 32 1481 32 2851
Fragments arranged by size:
2851 2377 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2514 2514
Size: 6805
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6805
PsiI TTA'TAA
Cuts at: 4375 4375
Size: 6805
PspOMI G'GGCC_C
Cuts at: 3749 3749
Size: 6805
PstI C_TGCA'G
Cuts at: 830 3331 3491 3556 4157 830
Size: 2501 160 65 601 3478
Fragments arranged by size:
3478 2501 601 160 65
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6805
SalI G'TCGA_C
Cuts at: 1936 4147 1936
Size: 2211 4594
SanDI GG'GwC_CC
Cuts at: 2514 2514
Size: 6805
SapI GCTCTTCn'nnn_
Cuts at: 2796 2796
Size: 6805
SbfI CC_TGCA'GG
Cuts at: 4157 4157
Size: 6805
ScaI AGT'ACT
Cuts at: 5053 6765 5053
Size: 1712 5093
SexAI A'CCwGG_T
Cuts at: 3428 3428
Size: 6805
SfcI C'TryA_G
Cuts at: 826 1045 3327 3487 3525 3552 4153 5288 5969
Size: 219 2282 160 38 27 601 1135 681
Cuts at: 5969 6160 826
Size: 191 1471
Fragments arranged by size:
2282 1471 1135 681 601 219 191 160
38 27
SfoI GGC'GCC
Cuts at: 2122 2122
Size: 6805
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6805
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6805
SmaI CCC'GGG
Cuts at: 1872 4132 1872
Size: 2260 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4909 5810 6072 820
Size: 197 926 2966 901 262 1553
Fragments arranged by size:
2966 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4102 4596 493
Size: 3609 494 2702
Fragments arranged by size:
3609 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6805
SphI G_CATG'C
Cuts at: 1132 3017 4163 1132
Size: 1885 1146 3774
Fragments arranged by size:
3774 1885 1146
SspI AAT'ATT
Cuts at: 5 52 4729 5
Size: 47 4677 2081
Fragments arranged by size:
4677 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6805
StyI C'CwwG_G
Cuts at: 513 1065 2946 3408 4244 513
Size: 552 1881 462 836 3074
Fragments arranged by size:
3074 1881 836 552 462
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4832 4849 5002 5187 5632 325
Size: 1255 3252 17 153 185 445 1498
Fragments arranged by size:
3252 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3665 5051 6763
Size: 274 80 33 51 1443 1688 1386 1712
Cuts at: 6763 96
Size: 138
Fragments arranged by size:
1712 1688 1443 1386 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2406 2438 6765 6797 2406
Size: 32 4327 32 2414
Fragments arranged by size:
4327 2414 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6805
XbaI T'CTAG_A
Cuts at: 4141 4141
Size: 6805
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2698 2698
Size: 6805
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6805
XmaI C'CCGG_G
Cuts at: 1870 4130 1870
Size: 2260 4545
XmnI GAAnn'nnTTC
Cuts at: 4934 4934
Size: 6805
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4613 276
Size: 53 83 186 936 3079 2468
Fragments arranged by size:
3079 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI PvuII RsrII SacI SacII SfiI SrfI
SwaI