Restriction map for
pFN21ASDA0941
(Circular) (Six-base) MAPSORT of: pFN21ASDA0941.seq Check: 3418 from: 1 to: 6265
pFN21ASDA0941 6265 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2311 2311
Size: 6265
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4075 278
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3608 1937
Size: 1671 4594
Acc65I G'GTAC_C
Cuts at: 2117 3586 2117
Size: 1469 4796
AclI AA'CG_TT
Cuts at: 1563 4392 4765 1563
Size: 2829 373 3063
Fragments arranged by size:
3063 2829 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2117 2765 4328 5712 5980 1632
Size: 485 648 1563 1384 268 1917
Fragments arranged by size:
1917 1563 1384 648 485 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6068
AflIII A'CryG_T
Cuts at: 1203 5164 6141 1203
Size: 3961 977 1327
Fragments arranged by size:
3961 1327 977
AgeI A'CCGG_T
Cuts at: 5096 5096
Size: 6265
AhdI GACnn_n'nnGTC
Cuts at: 4994 4994
Size: 6265
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6265
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6265
AlwNI CAG_nnn'CTG
Cuts at: 3400 4008 5580 3400
Size: 608 1572 4085
Fragments arranged by size:
4085 1572 608
ApaLI G'TGCA_C
Cuts at: 4322 5478 4322
Size: 1156 5109
ApoI r'AATT_y
Cuts at: 1493 2309 2618 2963 3031 3254 3545 3574 3800
Size: 816 309 345 68 223 291 29 226
Cuts at: 3800 3952 6061 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 816 345 309 291 226 223
152 68 29
AseI AT'TA_AT
Cuts at: 160 1032 4819 160
Size: 872 3787 1606
Fragments arranged by size:
3787 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
AvaI C'yCGr_G
Cuts at: 1870 1943 3452 3590 1870
Size: 73 1509 138 4545
Fragments arranged by size:
4545 1509 138 73
BamHI G'GATC_C
Cuts at: 3595 3962 3595
Size: 367 5898
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2117 2753 3586 5041 618
Size: 325 217 627 330 636 833 1455 1842
Fragments arranged by size:
1842 1455 833 636 627 330 325 217
BanII G_rGCy'C
Cuts at: 1391 3584 1391
Size: 2193 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2565 2724 2923 3321 928
Size: 971 666 159 199 398 3872
Fragments arranged by size:
3872 971 666 398 199 159
BbvCI CC'TCA_GC
Cuts at: 2679 2679
Size: 6265
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4456 4490 1903
Size: 34 2519 34 3678
Fragments arranged by size:
3678 2519 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4157 5367 4157
Size: 1210 5055
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6265
BfrBI ATG'CAT
Cuts at: 3194 3194
Size: 6265
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4876 136
Size: 107 122 71 631 605 3204 1525
Fragments arranged by size:
3204 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2092 2504 3134 6260 2092
Size: 412 630 3126 2097
Fragments arranged by size:
3126 2097 630 412
BlpI GC'TnA_GC
Cuts at: 3682 3682
Size: 6265
Bme1580I G_kGCm'C
Cuts at: 1790 4326 5482 1790
Size: 2536 1156 2573
Fragments arranged by size:
2573 2536 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6265
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3495 3996 4954
Size: 416 53 83 399 24 2078 501 958
Cuts at: 4954 442
Size: 1753
Fragments arranged by size:
2078 1753 958 501 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6265
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2230 2230
Size: 6265
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4925 1239
Size: 3686 2579
Bpu10I CC'TnA_GC
Cuts at: 2679 2679
Size: 6265
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2210 4390 5255 5553 2210
Size: 2180 865 298 2922
Fragments arranged by size:
2922 2180 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4928 882
Size: 617 3429 2219
Fragments arranged by size:
3429 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2065 3562 4056 493
Size: 711 861 1497 494 2702
Fragments arranged by size:
2702 1497 861 711 494
BsaBI GATnn'nnATC
Cuts at: 2389 6259 2389
Size: 3870 2395
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4072 4454 5089 275
Size: 53 83 186 936 2539 382 635 1451
Fragments arranged by size:
2539 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4698 5096 5370 5517 5928 4698
Size: 398 274 147 411 5035
Fragments arranged by size:
5035 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2491 2521 1178
Size: 30 165 30 1088 30 4922
Fragments arranged by size:
4922 1088 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2222 1186
Size: 1036 5229
BseYI C'CCAG_C
Cuts at: 2146 5468 5964 2146
Size: 3322 496 2447
Fragments arranged by size:
3322 2447 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2087 1163
Size: 573 351 5341
Fragments arranged by size:
5341 573 351
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3715 4476 5504 1540
Size: 275 186 1714 761 1028 2301
Fragments arranged by size:
2301 1714 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2082 4326 4411 5482 2082
Size: 2244 85 1071 2865
Fragments arranged by size:
2865 2244 1071 85
BsmI GAATG_Cn'
Cuts at: 2698 3776 3869 2698
Size: 1078 93 5094
Fragments arranged by size:
5094 1078 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2082 3584 4326 4411 5482 1391
Size: 399 292 1502 742 85 1071 2174
Fragments arranged by size:
2174 1502 1071 742 399 292 85
BspEI T'CCGG_A
Cuts at: 5928 5928
Size: 6265
BspHI T'CATG_A
Cuts at: 4154 5884 4154
Size: 1730 4535
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2059 2311 3620 844
Size: 1215 252 1309 3489
Fragments arranged by size:
3489 1309 1215 252
BsrBI CCG'CTC
Cuts at: 1394 1823 4152 1394
Size: 429 2329 3507
Fragments arranged by size:
3507 2329 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3316 4760 4934 65
Size: 1183 2068 1444 174 1396
Fragments arranged by size:
2068 1444 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4909 5096 1700
Size: 110 3099 187 2869
Fragments arranged by size:
3099 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6265
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6265
BssSI C'ACGA_G
Cuts at: 4325 5337 4325
Size: 1012 5253
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6265
BstBI TT'CG_AA
Cuts at: 2007 5069 2007
Size: 3062 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6265
BstYI r'GATC_y
Cuts at: 1972 2092 2504 3134 3158 3595 3962 4347 4364
Size: 120 412 630 24 437 367 385 17
Cuts at: 4364 5816 5902 5914 6260 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 630 437 412 385 367 346
120 86 24 17 12
Bsu36I CC'TnA_GG
Cuts at: 3320 3422 3320
Size: 102 6163
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5713
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5526
Fragments arranged by size:
5526 683 56
BtsI GCAGTG_nn'
Cuts at: 2972 3354 3781 4575 4595 2972
Size: 382 427 794 20 4642
Fragments arranged by size:
4642 794 427 382 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6265
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6230
DraI TTT'AAA
Cuts at: 3569 3569
Size: 6265
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5272 809
Size: 507 3956 1802
Fragments arranged by size:
3956 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3712 4601 6230
Size: 54 1425 50 275 18 1882 889 1629
Cuts at: 6230 8
Size: 43
Fragments arranged by size:
1882 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3712 1537
Size: 275 1900 4090
Fragments arranged by size:
4090 1900 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3378 4195 1353
Size: 2025 817 3423
Fragments arranged by size:
3423 2025 817
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4852 5226 5372 387
Size: 1064 138 3263 374 146 1280
Fragments arranged by size:
3263 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2117 2765 4328 4925 5712 5980 1239
Size: 393 485 648 1563 597 787 268 1524
Fragments arranged by size:
1563 1524 787 648 597 485 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2289 2675 2721 2289
Size: 386 46 5833
Fragments arranged by size:
5833 386 46
EcoRI G'AATT_C
Cuts at: 3574 3952 3574
Size: 378 5887
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3437 3437
Size: 6265
FspI TGC'GCA
Cuts at: 4771 4771
Size: 6265
HaeII r_GCGC'y
Cuts at: 1678 5412 1678
Size: 3734 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3167 3199 3215 3247 4906
Size: 32 135 32 1595 32 16 32 1659
Cuts at: 4906 4938 4980 5012 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1659 1595 135 42 32 32 32
32 32 32 16
HincII GTy'rAC
Cuts at: 669 1657 1938 3234 3609 3855 669
Size: 988 281 1296 375 246 3079
Fragments arranged by size:
3079 1296 988 375 281 246
HindIII A'AGCT_T
Cuts at: 748 2851 748
Size: 2103 4162
HpaI GTT'AAC
Cuts at: 3855 3855
Size: 6265
KpnI G_GTAC'C
Cuts at: 2121 3590 2121
Size: 1469 4796
MfeI C'AATT_G
Cuts at: 3048 3864 3048
Size: 816 5449
MluI A'CGCG_T
Cuts at: 6141 6141
Size: 6265
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2081 3326 5379 5563 1305
Size: 776 1245 2053 184 2007
Fragments arranged by size:
2053 2007 1245 776 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4443
Fragments arranged by size:
4443 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4223 4582 4741 518
Size: 648 3057 359 159 2042
Fragments arranged by size:
3057 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2146 3681 4358 5506 5751 1606
Size: 540 1535 677 1148 245 2120
Fragments arranged by size:
2120 1535 1148 677 540 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5713
NdeI CA'TA_TG
Cuts at: 387 2727 3294 3520 387
Size: 2340 567 226 3132
Fragments arranged by size:
3132 2340 567 226
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6265
NotI GC'GGCC_GC
Cuts at: 3712 3712
Size: 6265
NsiI A_TGCA'T
Cuts at: 3196 3196
Size: 6265
NspI r_CATG'y
Cuts at: 1132 1229 2602 3623 5168 1132
Size: 97 1373 1021 1545 2229
Fragments arranged by size:
2229 1545 1373 1021 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6265
PciI A'CATG_T
Cuts at: 5164 5164
Size: 6265
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2816 1108
Size: 1708 4557
PfoI T'CCnGG_A
Cuts at: 3330 3444 3530 3330
Size: 114 86 6065
Fragments arranged by size:
6065 114 86
PmeI GTTT'AAAC
Cuts at: 3569 3569
Size: 6265
PmlI CAC'GTG
Cuts at: 2065 2065
Size: 6265
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4334 4366 5847 5879 4334
Size: 32 1481 32 4720
Fragments arranged by size:
4720 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2289 2675 2721 2289
Size: 386 46 5833
Fragments arranged by size:
5833 386 46
PshAI GACnn'nnGTC
Cuts at: 1613 2689 1613
Size: 1076 5189
PsiI TTA'TAA
Cuts at: 3835 3835
Size: 6265
PspXI vC'TCGA_Gb
Cuts at: 3452 3452
Size: 6265
PstI C_TGCA'G
Cuts at: 830 3361 3617 830
Size: 2531 256 3478
Fragments arranged by size:
3478 2531 256
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6265
PvuII CAG'CTG
Cuts at: 2146 2146
Size: 6265
SalI G'TCGA_C
Cuts at: 1936 3607 1936
Size: 1671 4594
SanDI GG'GwC_CC
Cuts at: 2721 2721
Size: 6265
SbfI CC_TGCA'GG
Cuts at: 3617 3617
Size: 6265
ScaI AGT'ACT
Cuts at: 2162 2593 4513 6225 2162
Size: 431 1920 1712 2202
Fragments arranged by size:
2202 1920 1712 431
SfcI C'TryA_G
Cuts at: 826 1045 2172 3357 3392 3613 4748 5429 5620
Size: 219 1127 1185 35 221 1135 681 191
Cuts at: 5620 826
Size: 1471
Fragments arranged by size:
1471 1185 1135 1127 681 221 219 191
35
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6265
SmaI CCC'GGG
Cuts at: 1872 3592 1872
Size: 1720 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2189 3452 4369 5270 5532 820
Size: 197 926 246 1263 917 901 262 1553
Fragments arranged by size:
1553 1263 926 917 901 262 246 197
SnaBI TAC'GTA
Cuts at: 493 3562 4056 493
Size: 3069 494 2702
Fragments arranged by size:
3069 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6265
SphI G_CATG'C
Cuts at: 1132 3623 1132
Size: 2491 3774
SspI AAT'ATT
Cuts at: 5 52 2440 4189 5
Size: 47 2388 1749 2081
Fragments arranged by size:
2388 2081 1749 47
StuI AGG'CCT
Cuts at: 1517 2213 1517
Size: 696 5569
StyI C'CwwG_G
Cuts at: 513 1065 2978 3704 513
Size: 552 1913 726 3074
Fragments arranged by size:
3074 1913 726 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3452 4292 4309 4462 4647 5092 325
Size: 1255 1872 840 17 153 185 445 1498
Fragments arranged by size:
1872 1498 1255 840 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2160 2591 4511
Size: 274 80 33 51 1443 183 431 1920
Cuts at: 4511 6223 96
Size: 1712 138
Fragments arranged by size:
1920 1712 1443 431 274 183 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6225 6257 6225
Size: 32 6233
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6265
XbaI T'CTAG_A
Cuts at: 3601 3601
Size: 6265
XhoI C'TCGA_G
Cuts at: 1943 3452 1943
Size: 1509 4756
XmaI C'CCGG_G
Cuts at: 1870 3590 1870
Size: 1720 4545
XmnI GAAnn'nnTTC
Cuts at: 2560 3387 4394 2560
Size: 827 1007 4431
Fragments arranged by size:
4431 1007 827
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4073 276
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI
BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI
PsrI RsrII SacI SacII SapI SexAI SfiI SfoI
SrfI SwaI XcmI