Restriction map for
pFN21ASDA0940
(Circular) (Six-base) MAPSORT of: pFN21ASDA0940.seq Check: 293 from: 1 to: 6781
pFN21ASDA0940 6781 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4591 278
Size: 53 83 186 936 3055 2468
Fragments arranged by size:
3055 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3387 4124 1937
Size: 1450 737 4594
Fragments arranged by size:
4594 1450 737
Acc65I G'GTAC_C
Cuts at: 4102 4102
Size: 6781
AclI AA'CG_TT
Cuts at: 1563 4908 5281 1563
Size: 3345 373 3063
Fragments arranged by size:
3345 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3761 3809 4844 6228 6496 1632
Size: 2129 48 1035 1384 268 1917
Fragments arranged by size:
2129 1917 1384 1035 268 48
AfeI AGC'GCT
Cuts at: 3808 3808
Size: 6781
AflII C'TTAA_G
Cuts at: 820 1017 3250 820
Size: 197 2233 4351
Fragments arranged by size:
4351 2233 197
AflIII A'CryG_T
Cuts at: 1203 5680 6657 1203
Size: 4477 977 1327
Fragments arranged by size:
4477 1327 977
AgeI A'CCGG_T
Cuts at: 5612 5612
Size: 6781
AhdI GACnn_n'nnGTC
Cuts at: 5510 5510
Size: 6781
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6781
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6781
AlwNI CAG_nnn'CTG
Cuts at: 2201 2352 2708 4524 6096 2201
Size: 151 356 1816 1572 2886
Fragments arranged by size:
2886 1816 1572 356 151
ApaLI G'TGCA_C
Cuts at: 4838 5994 4838
Size: 1156 5625
ApoI r'AATT_y
Cuts at: 1493 3133 3558 4090 4316 4468 6577 1493
Size: 1640 425 532 226 152 2109 1697
Fragments arranged by size:
2109 1697 1640 532 425 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5335 160
Size: 872 4303 1606
Fragments arranged by size:
4303 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6781
AvaI C'yCGr_G
Cuts at: 1870 1943 3996 4106 1870
Size: 73 2053 110 4545
Fragments arranged by size:
4545 2053 110 73
BamHI G'GATC_C
Cuts at: 4111 4478 4111
Size: 367 6414
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2314 2363 2617 2866 4102
Size: 325 217 627 527 49 254 249 1236
Cuts at: 4102 5557 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1236 627 527 325 254 249
217 49
BanII G_rGCy'C
Cuts at: 1391 2310 4100 1391
Size: 919 1790 4072
Fragments arranged by size:
4072 1790 919
BbeI G_GCGC'C
Cuts at: 2621 2621
Size: 6781
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2452 2682 928
Size: 971 553 230 5027
Fragments arranged by size:
5027 971 553 230
BbvCI CC'TCA_GC
Cuts at: 2189 2283 2189
Size: 94 6687
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2905 2939 4972 5006 1903
Size: 34 968 34 2033 34 3678
Fragments arranged by size:
3678 2033 968 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2128 4673 5883 2128
Size: 2545 1210 3026
Fragments arranged by size:
3026 2545 1210
BclI T'GATC_A
Cuts at: 1548 3100 3229 3681 3877 1548
Size: 1552 129 452 196 4452
Fragments arranged by size:
4452 1552 452 196 129
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2210 2645 2732
Size: 107 122 71 631 605 538 435 87
Cuts at: 2732 5392 136
Size: 2660 1525
Fragments arranged by size:
2660 1525 631 605 538 435 122 107
87 71
BglII A'GATC_T
Cuts at: 6776 6776
Size: 6781
BlpI GC'TnA_GC
Cuts at: 4198 4198
Size: 6781
Bme1580I G_kGCm'C
Cuts at: 1790 2366 3905 4842 5998 1790
Size: 576 1539 937 1156 2573
Fragments arranged by size:
2573 1539 1156 937 576
BmgBI CAC'GTC
Cuts at: 1318 4057 1318
Size: 2739 4042
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2402 2450 4512
Size: 416 53 83 399 24 985 48 2062
Cuts at: 4512 5470 442
Size: 958 1753
Fragments arranged by size:
2062 1753 985 958 416 399 83 53
48 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6781
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3481 3481
Size: 6781
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2797 2896 4090 5441 1239
Size: 1558 99 1194 1351 2579
Fragments arranged by size:
2579 1558 1351 1194 99
Bpu10I CC'TnA_GC
Cuts at: 2189 2283 2189
Size: 94 6687
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2284 4906 5771 6069 2284
Size: 2622 865 298 2996
Fragments arranged by size:
2996 2622 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2144 5444 882
Size: 617 645 3300 2219
Fragments arranged by size:
3300 2219 645 617
BsaAI yAC'GTr
Cuts at: 493 1204 4078 4572 493
Size: 711 2874 494 2702
Fragments arranged by size:
2874 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 6775 6775
Size: 6781
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2618 4588 4970 5605
Size: 53 83 186 936 1085 1970 382 635
Cuts at: 5605 275
Size: 1451
Fragments arranged by size:
1970 1451 1085 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2991 3030 5214 5612 5886 6033 6444 2991
Size: 39 2184 398 274 147 411 3328
Fragments arranged by size:
3328 2184 411 398 274 147 39
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6556
Fragments arranged by size:
6556 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2134 2271 2647 1186
Size: 948 137 376 5320
Fragments arranged by size:
5320 948 376 137
BseYI C'CCAG_C
Cuts at: 2058 2064 2070 2194 2502 2609 3978 5984 6480
Size: 6 6 124 308 107 1369 2006 496
Cuts at: 6480 2058
Size: 2359
Fragments arranged by size:
2359 2006 1369 496 308 124 107 6
6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3839 1163
Size: 573 2103 4105
Fragments arranged by size:
4105 2103 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2838 4045 4231 4992 6020 1540
Size: 275 186 837 1207 186 761 1028 2301
Fragments arranged by size:
2301 1207 1028 837 761 275 186 186
BsiHKAI G_wGCw'C
Cuts at: 4842 4927 5998 4842
Size: 85 1071 5625
Fragments arranged by size:
5625 1071 85
BsiWI C'GTAC_G
Cuts at: 2649 2649
Size: 6781
BsmI GAATG_Cn'
Cuts at: 2773 3770 4292 4385 2773
Size: 997 522 93 5169
Fragments arranged by size:
5169 997 522 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2310 2366 3905 4100 4842 4927 5998
Size: 399 520 56 1539 195 742 85 1071
Cuts at: 5998 1391
Size: 2174
Fragments arranged by size:
2174 1539 1071 742 520 399 195 85
56
BspEI T'CCGG_A
Cuts at: 2991 6444 2991
Size: 3453 3328
BspHI T'CATG_A
Cuts at: 2667 3103 4670 6400 2667
Size: 436 1567 1730 3048
Fragments arranged by size:
3048 1730 1567 436
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3357 4136 844
Size: 2513 779 3489
Fragments arranged by size:
3489 2513 779
BsrBI CCG'CTC
Cuts at: 1394 1823 2251 2595 2878 2896 4668 1394
Size: 429 428 344 283 18 1772 3507
Fragments arranged by size:
3507 1772 429 428 344 283 18
BsrDI GCAATG_nn'
Cuts at: 65 1248 3684 3792 5276 5450 65
Size: 1183 2436 108 1484 174 1396
Fragments arranged by size:
2436 1484 1396 1183 174 108
BsrFI r'CCGG_y
Cuts at: 1700 1810 2182 2545 5425 5612 1700
Size: 110 372 363 2880 187 2869
Fragments arranged by size:
2880 2869 372 363 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6781
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6781
BssSI C'ACGA_G
Cuts at: 4841 5853 4841
Size: 1012 5769
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2708 794
Size: 1914 4867
BstBI TT'CG_AA
Cuts at: 2007 5585 2007
Size: 3578 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6781
BstYI r'GATC_y
Cuts at: 1972 4111 4478 4863 4880 6332 6418 6430 6776
Size: 2139 367 385 17 1452 86 12 346
Cuts at: 6776 1972
Size: 1977
Fragments arranged by size:
2139 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2301 2301
Size: 6781
BtgI C'CryG_G
Cuts at: 513 1065 2310 2358 2643 3223 3566 513
Size: 552 1245 48 285 580 343 3728
Fragments arranged by size:
3728 1245 580 552 343 285 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6042
Fragments arranged by size:
6042 683 56
BtsI GCAGTG_nn'
Cuts at: 2184 3351 4297 5091 5111 2184
Size: 1167 946 794 20 3854
Fragments arranged by size:
3854 1167 946 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6781
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6746
DraI TTT'AAA
Cuts at: 4085 4085
Size: 6781
DraIII CAC_nnn'GTG
Cuts at: 3911 3911
Size: 6781
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5788 809
Size: 507 4472 1802
Fragments arranged by size:
4472 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2653 3760 4228
Size: 54 1425 50 275 18 823 1107 468
Cuts at: 4228 5117 6746 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1107 889 823 468 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4228 1537
Size: 275 2416 4090
Fragments arranged by size:
4090 2416 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2432 4711 1353
Size: 1079 2279 3423
Fragments arranged by size:
3423 2279 1079
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5368 5742 5888 387
Size: 1064 138 3779 374 146 1280
Fragments arranged by size:
3779 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2797 2896 3761 3809 4090 4844 5441
Size: 393 1165 99 865 48 281 754 597
Cuts at: 5441 6228 6496 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1165 865 787 754 597 393 281
268 99 48
EcoNI CCTnn'n_nnAGG
Cuts at: 3114 3429 3114
Size: 315 6466
EcoO109I rG'GnC_Cy
Cuts at: 2821 3039 2821
Size: 218 6563
EcoRI G'AATT_C
Cuts at: 4090 4468 4090
Size: 378 6403
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3432 3432
Size: 6781
FspI TGC'GCA
Cuts at: 5287 5287
Size: 6781
HaeII r_GCGC'y
Cuts at: 1678 2593 2621 2640 3810 5928 1678
Size: 915 28 19 1170 2118 2531
Fragments arranged by size:
2531 2118 1170 915 28 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2257 2289 3171 3203 5422
Size: 32 135 32 685 32 882 32 2219
Cuts at: 5422 5454 5496 5528 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2219 882 685 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2413 2452 4125 4371 669
Size: 988 281 475 39 1673 246 3079
Fragments arranged by size:
3079 1673 988 475 281 246 39
HindIII A'AGCT_T
Cuts at: 748 3474 748
Size: 2726 4055
HpaI GTT'AAC
Cuts at: 4371 4371
Size: 6781
KasI G'GCGC_C
Cuts at: 2617 2617
Size: 6781
KpnI G_GTAC'C
Cuts at: 4106 4106
Size: 6781
MfeI C'AATT_G
Cuts at: 4380 4380
Size: 6781
MluI A'CGCG_T
Cuts at: 6657 6657
Size: 6781
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2814 3048 3295 5895 6079 1305
Size: 1509 234 247 2600 184 2007
Fragments arranged by size:
2600 2007 1509 247 234 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4959
Fragments arranged by size:
4959 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4739 5098 5257 518
Size: 648 3573 359 159 2042
Fragments arranged by size:
3573 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2083 2201 2312 2645 2708 2745 4024 4068
Size: 477 118 111 333 63 37 1279 44
Cuts at: 4068 4197 4874 6022 6267 1606
Size: 129 677 1148 245 2120
Fragments arranged by size:
2120 1279 1148 677 477 333 245 129
118 111 63 44 37
NaeI GCC'GGC
Cuts at: 2184 2547 2184
Size: 363 6418
NarI GG'CG_CC
Cuts at: 2618 2618
Size: 6781
NcoI C'CATG_G
Cuts at: 513 1065 3223 3566 513
Size: 552 2158 343 3728
Fragments arranged by size:
3728 2158 552 343
NdeI CA'TA_TG
Cuts at: 387 3865 387
Size: 3478 3303
NgoMIV G'CCGG_C
Cuts at: 2182 2545 2182
Size: 363 6418
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6781
NotI GC'GGCC_GC
Cuts at: 4228 4228
Size: 6781
NspI r_CATG'y
Cuts at: 1132 1229 4139 5684 1132
Size: 97 2910 1545 2229
Fragments arranged by size:
2910 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2796 1348
Size: 1448 5333
PciI A'CATG_T
Cuts at: 5680 5680
Size: 6781
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2796 2970 1108
Size: 1688 174 4919
Fragments arranged by size:
4919 1688 174
PfoI T'CCnGG_A
Cuts at: 2969 3145 3210 2969
Size: 176 65 6540
Fragments arranged by size:
6540 176 65
PmeI GTTT'AAAC
Cuts at: 4085 4085
Size: 6781
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4850 4882 6363 6395 4850
Size: 32 1481 32 5236
Fragments arranged by size:
5236 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2821 2821
Size: 6781
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6781
PsiI TTA'TAA
Cuts at: 4351 4351
Size: 6781
PstI C_TGCA'G
Cuts at: 830 2811 3960 4133 830
Size: 1981 1149 173 3478
Fragments arranged by size:
3478 1981 1149 173
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6781
PvuII CAG'CTG
Cuts at: 2745 2745
Size: 6781
RsrII CG'GwC_CG
Cuts at: 2994 2994
Size: 6781
SacII CC_GC'GG
Cuts at: 2313 2646 2313
Size: 333 6448
SalI G'TCGA_C
Cuts at: 1936 4123 1936
Size: 2187 4594
SanDI GG'GwC_CC
Cuts at: 2821 2821
Size: 6781
SapI GCTCTTCn'nnn_
Cuts at: 2432 2432
Size: 6781
SbfI CC_TGCA'GG
Cuts at: 2811 4133 2811
Size: 1322 5459
ScaI AGT'ACT
Cuts at: 5029 6741 5029
Size: 1712 5069
SexAI A'CCwGG_T
Cuts at: 2351 2351
Size: 6781
SfcI C'TryA_G
Cuts at: 826 1045 2807 3956 4129 5264 5945 6136 826
Size: 219 1762 1149 173 1135 681 191 1471
Fragments arranged by size:
1762 1471 1149 1135 681 219 191 173
SfoI GGC'GCC
Cuts at: 2619 2619
Size: 6781
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6781
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6781
SmaI CCC'GGG
Cuts at: 1872 4108 1872
Size: 2236 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2263 3250 4885 5786 6048 820
Size: 197 926 320 987 1635 901 262 1553
Fragments arranged by size:
1635 1553 987 926 901 320 262 197
SnaBI TAC'GTA
Cuts at: 493 4078 4572 493
Size: 3585 494 2702
Fragments arranged by size:
3585 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6781
SphI G_CATG'C
Cuts at: 1132 4139 1132
Size: 3007 3774
SspI AAT'ATT
Cuts at: 5 52 3160 4705 5
Size: 47 3108 1545 2081
Fragments arranged by size:
3108 2081 1545 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6781
StyI C'CwwG_G
Cuts at: 513 1065 3223 3566 4220 513
Size: 552 2158 343 654 3074
Fragments arranged by size:
3074 2158 654 552 343
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2840 2846 3011 4808 4825 4978 5163
Size: 1255 1260 6 165 1797 17 153 185
Cuts at: 5163 5608 325
Size: 445 1498
Fragments arranged by size:
1797 1498 1260 1255 445 185 165 153
17 6
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3469 5027 6739
Size: 274 80 33 51 1443 1492 1558 1712
Cuts at: 6739 96
Size: 138
Fragments arranged by size:
1712 1558 1492 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3562 3594 6741 6773 3562
Size: 32 3147 32 3570
Fragments arranged by size:
3570 3147 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6781
XbaI T'CTAG_A
Cuts at: 3299 4117 3299
Size: 818 5963
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2971 2971
Size: 6781
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6781
XmaI C'CCGG_G
Cuts at: 1870 4106 1870
Size: 2236 4545
XmnI GAAnn'nnTTC
Cuts at: 4910 4910
Size: 6781
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4589 276
Size: 53 83 186 936 3055 2468
Fragments arranged by size:
3055 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BfrBI BsmBI
BstXI BstZ17I EcoICRI EcoRV FseI FspAI NruI NsiI
PacI PmlI PspOMI PspXI PsrI SacI SfiI SrfI
SwaI