Restriction map for
pFN21ASDA0939
(Circular) (Six-base) MAPSORT of: pFN21ASDA0939.seq Check: 3705 from: 1 to: 6472
pFN21ASDA0939 6472 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3489 4282 278
Size: 53 83 186 936 1953 793 2468
Fragments arranged by size:
2468 1953 936 793 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3815 1937
Size: 1878 4594
Acc65I G'GTAC_C
Cuts at: 3793 3793
Size: 6472
AclI AA'CG_TT
Cuts at: 1563 4599 4972 1563
Size: 3036 373 3063
Fragments arranged by size:
3063 3036 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2197 2763 2900 4535 5919 6187 1632
Size: 565 566 137 1635 1384 268 1917
Fragments arranged by size:
1917 1635 1384 566 565 268 137
AfeI AGC'GCT
Cuts at: 2841 2841
Size: 6472
AflII C'TTAA_G
Cuts at: 820 1017 3593 820
Size: 197 2576 3699
Fragments arranged by size:
3699 2576 197
AflIII A'CryG_T
Cuts at: 1203 2394 3101 5371 6348 1203
Size: 1191 707 2270 977 1327
Fragments arranged by size:
2270 1327 1191 977 707
AgeI A'CCGG_T
Cuts at: 5303 5303
Size: 6472
AhdI GACnn_n'nnGTC
Cuts at: 5201 5201
Size: 6472
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6472
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3440 1184
Size: 2256 4216
AlwNI CAG_nnn'CTG
Cuts at: 3760 4215 5787 3760
Size: 455 1572 4445
Fragments arranged by size:
4445 1572 455
ApaI G_GGCC'C
Cuts at: 3731 3731
Size: 6472
ApaLI G'TGCA_C
Cuts at: 4529 5685 4529
Size: 1156 5316
ApoI r'AATT_y
Cuts at: 1493 2538 3156 3235 3781 4007 4159 6268 1493
Size: 1045 618 79 546 226 152 2109 1697
Fragments arranged by size:
2109 1697 1045 618 546 226 152 79
AseI AT'TA_AT
Cuts at: 160 1032 5026 160
Size: 872 3994 1606
Fragments arranged by size:
3994 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6472
AvaI C'yCGr_G
Cuts at: 1870 1943 3797 1870
Size: 73 1854 4545
Fragments arranged by size:
4545 1854 73
BamHI G'GATC_C
Cuts at: 3802 4169 3802
Size: 367 6105
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3368 3793 5248 618
Size: 325 217 627 1581 425 1455 1842
Fragments arranged by size:
1842 1581 1455 627 425 325 217
BanII G_rGCy'C
Cuts at: 1391 3345 3549 3731 3791 1391
Size: 1954 204 182 60 4072
Fragments arranged by size:
4072 1954 204 182 60
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5501
BbvCI CC'TCA_GC
Cuts at: 3494 3494
Size: 6472
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2104 2138 4663 4697 1903
Size: 34 167 34 2525 34 3678
Fragments arranged by size:
3678 2525 167 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4364 5574 4364
Size: 1210 5262
BclI T'GATC_A
Cuts at: 1548 2928 1548
Size: 1380 5092
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2953 3735 5083
Size: 107 122 71 631 605 1281 782 1348
Cuts at: 5083 136
Size: 1525
Fragments arranged by size:
1525 1348 1281 782 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6467 6467
Size: 6472
BlpI GC'TnA_GC
Cuts at: 3889 3889
Size: 6472
Bme1580I G_kGCm'C
Cuts at: 1790 3731 4533 5689 1790
Size: 1941 802 1156 2573
Fragments arranged by size:
2573 1941 1156 802
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6472
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3038 4203 5161
Size: 416 53 83 399 24 1621 1165 958
Cuts at: 5161 442
Size: 1753
Fragments arranged by size:
1753 1621 1165 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6472
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3359 5132 1239
Size: 2120 1773 2579
Fragments arranged by size:
2579 2120 1773
Bpu10I CC'TnA_GC
Cuts at: 3320 3494 3320
Size: 174 6298
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2914 4597 5462 5760 2914
Size: 1683 865 298 3626
Fragments arranged by size:
3626 1683 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5135 882
Size: 617 3636 2219
Fragments arranged by size:
3636 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3769 4263 493
Size: 711 2565 494 2702
Fragments arranged by size:
2702 2565 711 494
BsaBI GATnn'nnATC
Cuts at: 3275 6466 3275
Size: 3191 3281
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3456 3486 3615 4279
Size: 53 83 186 936 1923 30 129 664
Cuts at: 4279 4661 5296 275
Size: 382 635 1451
Fragments arranged by size:
1923 1451 936 664 635 382 186 129
83 53 30
BsaWI w'CCGG_w
Cuts at: 4905 5303 5577 5724 6135 4905
Size: 398 274 147 411 5242
Fragments arranged by size:
5242 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2109 2139 3524 3545 3554
Size: 30 165 30 706 30 1385 21 9
Cuts at: 3554 3575 1178
Size: 21 4075
Fragments arranged by size:
4075 1385 706 165 30 30 30 21
21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2060 2198 2396 3569 3572 3728 1186
Size: 874 138 198 1173 3 156 3930
Fragments arranged by size:
3930 1173 874 198 156 138 3
BseYI C'CCAG_C
Cuts at: 2175 3409 5675 6171 2175
Size: 1234 2266 496 2476
Fragments arranged by size:
2476 2266 1234 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2177 3650 1163
Size: 573 441 1473 3985
Fragments arranged by size:
3985 1473 573 441
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3922 4683 5711 1540
Size: 275 186 1921 761 1028 2301
Fragments arranged by size:
2301 1921 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2127 2676 3393 3537 3549 4533 4618 5689 2127
Size: 549 717 144 12 984 85 1071 2910
Fragments arranged by size:
2910 1071 984 717 549 144 85 12
BsmI GAATG_Cn'
Cuts at: 3983 4076 3983
Size: 93 6379
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2127 2676 3345 3393 3537 3549 3731
Size: 399 337 549 669 48 144 12 182
Cuts at: 3731 3791 4533 4618 5689 1391
Size: 60 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 669 549 399 337 182
144 85 60 48 12
BspEI T'CCGG_A
Cuts at: 6135 6135
Size: 6472
BspHI T'CATG_A
Cuts at: 2068 4361 6091 2068
Size: 2293 1730 2449
Fragments arranged by size:
2449 2293 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3672 3827 844
Size: 2828 155 3489
Fragments arranged by size:
3489 2828 155
BsrBI CCG'CTC
Cuts at: 1394 1823 3203 4359 1394
Size: 429 1380 1156 3507
Fragments arranged by size:
3507 1380 1156 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4967 5141 65
Size: 1183 3719 174 1396
Fragments arranged by size:
3719 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5116 5303 1700
Size: 110 3306 187 2869
Fragments arranged by size:
3306 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6472
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6472
BssSI C'ACGA_G
Cuts at: 4532 5544 4532
Size: 1012 5460
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3760 794
Size: 2966 3506
BstBI TT'CG_AA
Cuts at: 2007 5276 2007
Size: 3269 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6472
BstYI r'GATC_y
Cuts at: 1972 3054 3802 4169 4554 4571 6023 6109 6121
Size: 1082 748 367 385 17 1452 86 12
Cuts at: 6121 6467 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1082 748 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 3081 3345 3670 513
Size: 552 957 1059 264 325 3315
Fragments arranged by size:
3315 1059 957 552 325 264
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5733
Fragments arranged by size:
5733 683 56
BtsI GCAGTG_nn'
Cuts at: 3988 4782 4802 3988
Size: 794 20 5658
Fragments arranged by size:
5658 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6472
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2086 2121 529
Size: 35 1522 35 4880
Fragments arranged by size:
4880 1522 35 35
DraI TTT'AAA
Cuts at: 2350 3776 2350
Size: 1426 5046
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5479 809
Size: 507 4163 1802
Fragments arranged by size:
4163 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2631 2988 3919
Size: 54 1425 50 275 18 801 357 931
Cuts at: 3919 4808 6437 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 931 889 801 357 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3919 1537
Size: 275 2107 4090
Fragments arranged by size:
4090 2107 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2037 3398 4402 1353
Size: 684 1361 1004 3423
Fragments arranged by size:
3423 1361 1004 684
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2054 5059 5433 5579 387
Size: 1064 138 465 3005 374 146 1280
Fragments arranged by size:
3005 1280 1064 465 374 146 138
EcoICRI GAG'CTC
Cuts at: 3547 3547
Size: 6472
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2197 2763 2900 3359 4535 5132 5919
Size: 393 565 566 137 459 1176 597 787
Cuts at: 5919 6187 1239
Size: 268 1524
Fragments arranged by size:
1524 1176 787 597 566 565 459 393
268 137
EcoO109I rG'GnC_Cy
Cuts at: 3589 3661 3727 3728 3589
Size: 72 66 1 6333
Fragments arranged by size:
6333 72 66 1
EcoRI G'AATT_C
Cuts at: 3781 4159 3781
Size: 378 6094
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2476 2476
Size: 6472
FspI TGC'GCA
Cuts at: 4978 4978
Size: 6472
HaeII r_GCGC'y
Cuts at: 1678 2843 5619 1678
Size: 1165 2776 2531
Fragments arranged by size:
2776 2531 1165
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2174 2206 2552 2584 2702
Size: 32 135 32 602 32 346 32 118
Cuts at: 2702 2734 3524 3556 3676 3708 5113 5145 5187
Size: 32 790 32 120 32 1405 32 42
Cuts at: 5187 5219 1373
Size: 32 2626
Fragments arranged by size:
2626 1405 790 602 346 135 120 118
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3490 3816 4062 669
Size: 988 281 1552 326 246 3079
Fragments arranged by size:
3079 1552 988 326 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6472
HpaI GTT'AAC
Cuts at: 4062 4062
Size: 6472
KpnI G_GTAC'C
Cuts at: 3797 3797
Size: 6472
MfeI C'AATT_G
Cuts at: 4071 4071
Size: 6472
MluI A'CGCG_T
Cuts at: 6348 6348
Size: 6472
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3510 3522 5586 5770 1305
Size: 2205 12 2064 184 2007
Fragments arranged by size:
2205 2064 2007 184 12
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2633 2990 10
Size: 54 1425 343 801 357 3492
Fragments arranged by size:
3492 1425 801 357 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2246 3011 3683 4430 4789 4948 518
Size: 648 1080 765 672 747 359 159 2042
Fragments arranged by size:
2042 1080 765 747 672 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2196 2741 3888 4565 5713 5958 1606
Size: 590 545 1147 677 1148 245 2120
Fragments arranged by size:
2120 1148 1147 677 590 545 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 3345 513
Size: 552 957 1323 3640
Fragments arranged by size:
3640 1323 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6472
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6472
NotI GC'GGCC_GC
Cuts at: 3919 3919
Size: 6472
NspI r_CATG'y
Cuts at: 1132 1229 2398 2683 3105 3830 5375 1132
Size: 97 1169 285 422 725 1545 2229
Fragments arranged by size:
2229 1545 1169 725 422 285 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6472
PciI A'CATG_T
Cuts at: 2394 3101 5371 2394
Size: 707 2270 3495
Fragments arranged by size:
3495 2270 707
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3705 1108
Size: 2597 3875
PfoI T'CCnGG_A
Cuts at: 3243 3243
Size: 6472
PmeI GTTT'AAAC
Cuts at: 3776 3776
Size: 6472
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2036 2068 4541 4573 6054 6086 2036
Size: 32 2473 32 1481 32 2422
Fragments arranged by size:
2473 2422 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3589 3661 3589
Size: 72 6400
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6472
PsiI TTA'TAA
Cuts at: 2333 4042 2333
Size: 1709 4763
PspOMI G'GGCC_C
Cuts at: 3727 3727
Size: 6472
PstI C_TGCA'G
Cuts at: 830 2839 3824 830
Size: 2009 985 3478
Fragments arranged by size:
3478 2009 985
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6472
PvuII CAG'CTG
Cuts at: 2741 2741
Size: 6472
SacI G_AGCT'C
Cuts at: 3549 3549
Size: 6472
SalI G'TCGA_C
Cuts at: 1936 3814 1936
Size: 1878 4594
SapI GCTCTTCn'nnn_
Cuts at: 3398 3398
Size: 6472
SbfI CC_TGCA'GG
Cuts at: 3824 3824
Size: 6472
ScaI AGT'ACT
Cuts at: 4720 6432 4720
Size: 1712 4760
SfcI C'TryA_G
Cuts at: 826 1045 2835 3820 4955 5636 5827 826
Size: 219 1790 985 1135 681 191 1471
Fragments arranged by size:
1790 1471 1135 985 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6472
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6472
SmaI CCC'GGG
Cuts at: 1872 3799 1872
Size: 1927 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2893 3593 4576 5477 5739 820
Size: 197 926 950 700 983 901 262 1553
Fragments arranged by size:
1553 983 950 926 901 700 262 197
SnaBI TAC'GTA
Cuts at: 493 3769 4263 493
Size: 3276 494 2702
Fragments arranged by size:
3276 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6472
SphI G_CATG'C
Cuts at: 1132 3830 1132
Size: 2698 3774
SspI AAT'ATT
Cuts at: 5 52 2473 4396 5
Size: 47 2421 1923 2081
Fragments arranged by size:
2421 2081 1923 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6472
StyI C'CwwG_G
Cuts at: 513 1065 2022 2910 3345 3459 3911 513
Size: 552 957 888 435 114 452 3074
Fragments arranged by size:
3074 957 888 552 452 435 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2769 3065 4499 4516 4669 4854 5299
Size: 1255 1189 296 1434 17 153 185 445
Cuts at: 5299 325
Size: 1498
Fragments arranged by size:
1498 1434 1255 1189 445 296 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3187 4718 6430
Size: 274 80 33 51 1443 1210 1531 1712
Cuts at: 6430 96
Size: 138
Fragments arranged by size:
1712 1531 1443 1210 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6432 6464 6432
Size: 32 6440
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6472
XbaI T'CTAG_A
Cuts at: 3808 3808
Size: 6472
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3406 3406
Size: 6472
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6472
XmaI C'CCGG_G
Cuts at: 1870 3797 1870
Size: 1927 4545
XmnI GAAnn'nnTTC
Cuts at: 2382 2700 3239 3632 4601 2382
Size: 318 539 393 969 4253
Fragments arranged by size:
4253 969 539 393 318
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3487 4280 276
Size: 53 83 186 936 1953 793 2468
Fragments arranged by size:
2468 1953 936 793 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI