Restriction map for
pFN21ASDA0936
(Circular) (Six-base) MAPSORT of: pFN21ASDA0936.seq Check: 6346 from: 1 to: 6625
pFN21ASDA0936 6625 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2995 2995
Size: 6625
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2571 3437 4435 278
Size: 53 83 186 936 1035 866 998 2468
Fragments arranged by size:
2468 1035 998 936 866 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2543 3968 1937
Size: 606 1425 4594
Fragments arranged by size:
4594 1425 606
Acc65I G'GTAC_C
Cuts at: 3946 3946
Size: 6625
AclI AA'CG_TT
Cuts at: 1563 4752 5125 1563
Size: 3189 373 3063
Fragments arranged by size:
3189 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2718 2801 2897 3407 3591 3800 4688 6072
Size: 1086 83 96 510 184 209 888 1384
Cuts at: 6072 6340 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1086 888 510 268 209 184
96 83
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6428
AflIII A'CryG_T
Cuts at: 1203 5524 6501 1203
Size: 4321 977 1327
Fragments arranged by size:
4321 1327 977
AgeI A'CCGG_T
Cuts at: 5456 5456
Size: 6625
AhdI GACnn_n'nnGTC
Cuts at: 5354 5354
Size: 6625
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6625
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6625
AlwNI CAG_nnn'CTG
Cuts at: 2803 4368 5940 2803
Size: 1565 1572 3488
Fragments arranged by size:
3488 1572 1565
ApaLI G'TGCA_C
Cuts at: 4682 5838 4682
Size: 1156 5469
ApoI r'AATT_y
Cuts at: 1493 2135 2655 3018 3557 3637 3934 4160 4312
Size: 642 520 363 539 80 297 226 152
Cuts at: 4312 6421 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 642 539 520 363 297 226
152 80
AseI AT'TA_AT
Cuts at: 160 1032 5179 160
Size: 872 4147 1606
Fragments arranged by size:
4147 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6625
AvaI C'yCGr_G
Cuts at: 1870 1943 2051 2463 3950 1870
Size: 73 108 412 1487 4545
Fragments arranged by size:
4545 1487 412 108 73
BamHI G'GATC_C
Cuts at: 2824 3955 4322 2824
Size: 1131 367 5127
Fragments arranged by size:
5127 1131 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3946 5401 618
Size: 325 217 627 2159 1455 1842
Fragments arranged by size:
2159 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2524 3944 1391
Size: 1133 1420 4072
Fragments arranged by size:
4072 1420 1133
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5654
BbvCI CC'TCA_GC
Cuts at: 3311 3311
Size: 6625
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4816 4850 1903
Size: 34 2879 34 3678
Fragments arranged by size:
3678 2879 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4517 5727 4517
Size: 1210 5415
BclI T'GATC_A
Cuts at: 1548 2239 1548
Size: 691 5934
BfrBI ATG'CAT
Cuts at: 2157 2157
Size: 6625
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5236 136
Size: 107 122 71 631 605 3564 1525
Fragments arranged by size:
3564 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3467 6620 3467
Size: 3153 3472
BlpI GC'TnA_GC
Cuts at: 4042 4042
Size: 6625
Bme1580I G_kGCm'C
Cuts at: 1790 3410 3424 4686 5842 1790
Size: 1620 14 1262 1156 2573
Fragments arranged by size:
2573 1620 1262 1156 14
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6625
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3901 4356 5314
Size: 416 53 83 399 24 2484 455 958
Cuts at: 5314 442
Size: 1753
Fragments arranged by size:
2484 1753 958 455 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2784 1056
Size: 1728 4897
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2508 2646 3073 3098 3212 3843 5285 1239
Size: 1269 138 427 25 114 631 1442 2579
Fragments arranged by size:
2579 1442 1269 631 427 138 114 25
Bpu10I CC'TnA_GC
Cuts at: 3311 3311
Size: 6625
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4750 5615 5913 4750
Size: 865 298 5462
Fragments arranged by size:
5462 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3091 3784 5288 882
Size: 617 1592 693 1504 2219
Fragments arranged by size:
2219 1592 1504 693 617
BsaAI yAC'GTr
Cuts at: 493 1204 3066 3922 4416 493
Size: 711 1862 856 494 2702
Fragments arranged by size:
2702 1862 856 711 494
BsaBI GATnn'nnATC
Cuts at: 6619 6619
Size: 6625
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2568 3434 4432 4814
Size: 53 83 186 936 1035 866 998 382
Cuts at: 4814 5449 275
Size: 635 1451
Fragments arranged by size:
1451 1035 998 936 866 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5058 5456 5730 5877 6288 5058
Size: 398 274 147 411 5395
Fragments arranged by size:
5395 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2162 2192 2540 2570 1178
Size: 30 165 30 759 30 348 30 5233
Fragments arranged by size:
5233 759 348 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2405 1186
Size: 1219 5406
BseYI C'CCAG_C
Cuts at: 2080 2668 2991 3822 3867 5828 6324 2080
Size: 588 323 831 45 1961 496 2381
Fragments arranged by size:
2381 1961 831 588 496 323 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6052
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4075 4836 5864 1540
Size: 275 186 2074 761 1028 2301
Fragments arranged by size:
2301 2074 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3198 4686 4771 5842 3198
Size: 1488 85 1071 3981
Fragments arranged by size:
3981 1488 1071 85
BsmI GAATG_Cn'
Cuts at: 2159 4136 4229 2159
Size: 1977 93 4555
Fragments arranged by size:
4555 1977 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2524 3198 3410 3424 3944 4686 4771
Size: 399 734 674 212 14 520 742 85
Cuts at: 4771 5842 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 734 674 520 399 212
85 14
BspEI T'CCGG_A
Cuts at: 6288 6288
Size: 6625
BspHI T'CATG_A
Cuts at: 4514 6244 4514
Size: 1730 4895
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2995 3354 3980 844
Size: 2151 359 626 3489
Fragments arranged by size:
3489 2151 626 359
BsrBI CCG'CTC
Cuts at: 1394 1823 4512 1394
Size: 429 2689 3507
Fragments arranged by size:
3507 2689 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2727 5120 5294 65
Size: 1183 1479 2393 174 1396
Fragments arranged by size:
2393 1479 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5269 5456 1700
Size: 110 3459 187 2869
Fragments arranged by size:
3459 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6625
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6625
BssSI C'ACGA_G
Cuts at: 4685 5697 4685
Size: 1012 5613
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6625
BstBI TT'CG_AA
Cuts at: 2007 5429 2007
Size: 3422 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6625
BstYI r'GATC_y
Cuts at: 1972 2824 3467 3955 4322 4707 4724 6176 6262
Size: 852 643 488 367 385 17 1452 86
Cuts at: 6262 6274 6620 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 852 643 488 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2537 2609 2537
Size: 72 6553
BtgI C'CryG_G
Cuts at: 513 1065 3575 3851 513
Size: 552 2510 276 3287
Fragments arranged by size:
3287 2510 552 276
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5886
Fragments arranged by size:
5886 683 56
BtsI GCAGTG_nn'
Cuts at: 2791 4141 4935 4955 2791
Size: 1350 794 20 4461
Fragments arranged by size:
4461 1350 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6625
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6590
DraI TTT'AAA
Cuts at: 2184 3929 2184
Size: 1745 4880
DraIII CAC_nnn'GTG
Cuts at: 2744 2744
Size: 6625
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5632 809
Size: 507 4316 1802
Fragments arranged by size:
4316 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2735 3880 4072
Size: 54 1425 50 275 18 905 1145 192
Cuts at: 4072 4961 6590 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1145 905 889 275 192 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4072 1537
Size: 275 2260 4090
Fragments arranged by size:
4090 2260 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2624 4555 1353
Size: 1271 1931 3423
Fragments arranged by size:
3423 1931 1271
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5212 5586 5732 387
Size: 1064 138 3623 374 146 1280
Fragments arranged by size:
3623 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2508 2646 2718 2801 2897 3073 3098
Size: 393 876 138 72 83 96 176 25
Cuts at: 3098 3212 3407 3591 3800 3843 4688 5285 6072
Size: 114 195 184 209 43 845 597 787
Cuts at: 6072 6340 1239
Size: 268 1524
Fragments arranged by size:
1524 876 845 787 597 393 268 209
195 184 176 138 114 96 83 72
43 25
EcoNI CCTnn'n_nnAGG
Cuts at: 2168 2948 2168
Size: 780 5845
EcoRI G'AATT_C
Cuts at: 3934 4312 3934
Size: 378 6247
EcoRV GAT'ATC
Cuts at: 2864 2864
Size: 6625
FspI TGC'GCA
Cuts at: 5131 5131
Size: 6625
HaeII r_GCGC'y
Cuts at: 1678 5772 1678
Size: 4094 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2162 2194 2486 2518 3424
Size: 32 135 32 590 32 292 32 906
Cuts at: 3424 3456 3781 3813 5266 5298 5340 5372 1373
Size: 32 325 32 1453 32 42 32 2626
Fragments arranged by size:
2626 1453 906 590 325 292 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3255 3969 4215 669
Size: 988 281 1317 714 246 3079
Fragments arranged by size:
3079 1317 988 714 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6625
HpaI GTT'AAC
Cuts at: 4215 4215
Size: 6625
KpnI G_GTAC'C
Cuts at: 3950 3950
Size: 6625
MfeI C'AATT_G
Cuts at: 4224 4224
Size: 6625
MluI A'CGCG_T
Cuts at: 6501 6501
Size: 6625
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2860 5739 5923 1305
Size: 1555 2879 184 2007
Fragments arranged by size:
2879 2007 1555 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2737 10
Size: 54 1425 343 905 3898
Fragments arranged by size:
3898 1425 905 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2202 3201 4583 4942 5101 518
Size: 648 1036 999 1382 359 159 2042
Fragments arranged by size:
2042 1382 1036 999 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3185 3445 4041 4718 5866 6111 1606
Size: 1579 260 596 677 1148 245 2120
Fragments arranged by size:
2120 1579 1148 677 596 260 245
NcoI C'CATG_G
Cuts at: 513 1065 3575 513
Size: 552 2510 3563
Fragments arranged by size:
3563 2510 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6625
NheI G'CTAG_C
Cuts at: 1052 2780 1052
Size: 1728 4897
NotI GC'GGCC_GC
Cuts at: 4072 4072
Size: 6625
NsiI A_TGCA'T
Cuts at: 2159 2159
Size: 6625
NspI r_CATG'y
Cuts at: 1132 1229 2815 3983 5528 1132
Size: 97 1586 1168 1545 2229
Fragments arranged by size:
2229 1586 1545 1168 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6625
PciI A'CATG_T
Cuts at: 5524 5524
Size: 6625
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2668 3878 1108
Size: 1560 1210 3855
Fragments arranged by size:
3855 1560 1210
PfoI T'CCnGG_A
Cuts at: 2943 3089 3114 2943
Size: 146 25 6454
Fragments arranged by size:
6454 146 25
PmeI GTTT'AAAC
Cuts at: 3929 3929
Size: 6625
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4694 4726 6207 6239 4694
Size: 32 1481 32 5080
Fragments arranged by size:
5080 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6625
PsiI TTA'TAA
Cuts at: 4195 4195
Size: 6625
PstI C_TGCA'G
Cuts at: 830 3365 3475 3977 830
Size: 2535 110 502 3478
Fragments arranged by size:
3478 2535 502 110
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6625
PvuII CAG'CTG
Cuts at: 3185 3185
Size: 6625
SalI G'TCGA_C
Cuts at: 1936 3967 1936
Size: 2031 4594
SbfI CC_TGCA'GG
Cuts at: 3977 3977
Size: 6625
ScaI AGT'ACT
Cuts at: 2532 4873 6585 2532
Size: 2341 1712 2572
Fragments arranged by size:
2572 2341 1712
SfcI C'TryA_G
Cuts at: 826 1045 2551 3361 3471 3973 5108 5789 5980
Size: 219 1506 810 110 502 1135 681 191
Cuts at: 5980 826
Size: 1471
Fragments arranged by size:
1506 1471 1135 810 681 502 219 191
110
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6625
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6625
SmaI CCC'GGG
Cuts at: 1872 3952 1872
Size: 2080 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4729 5630 5892 820
Size: 197 926 2786 901 262 1553
Fragments arranged by size:
2786 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3922 4416 493
Size: 3429 494 2702
Fragments arranged by size:
3429 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6625
SphI G_CATG'C
Cuts at: 1132 3983 1132
Size: 2851 3774
SspI AAT'ATT
Cuts at: 5 52 2649 4549 5
Size: 47 2597 1900 2081
Fragments arranged by size:
2597 2081 1900 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6625
StyI C'CwwG_G
Cuts at: 513 1065 3575 4064 513
Size: 552 2510 489 3074
Fragments arranged by size:
3074 2510 552 489
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2901 3117 3443 3836 4652 4669 4822
Size: 1255 1321 216 326 393 816 17 153
Cuts at: 4822 5007 5452 325
Size: 185 445 1498
Fragments arranged by size:
1498 1321 1255 816 445 393 326 216
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2262 2530 4871
Size: 274 80 33 51 1443 285 268 2341
Cuts at: 4871 6583 96
Size: 1712 138
Fragments arranged by size:
2341 1712 1443 285 274 268 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6585 6617 6585
Size: 32 6593
Tth111I GACn'n_nGTC
Cuts at: 1316 3616 1316
Size: 2300 4325
XbaI T'CTAG_A
Cuts at: 3961 3961
Size: 6625
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3573 3875 3573
Size: 302 6323
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6625
XmaI C'CCGG_G
Cuts at: 1870 3950 1870
Size: 2080 4545
XmnI GAAnn'nnTTC
Cuts at: 3022 3561 3604 4754 3022
Size: 539 43 1150 4893
Fragments arranged by size:
4893 1150 539 43
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2569 3435 4433 276
Size: 53 83 186 936 1035 866 998 2468
Fragments arranged by size:
2468 1035 998 936 866 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstXI BstZ17I EcoICRI EcoO109I FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI