Restriction map for
pFN21ASDA0924
(Circular) (Six-base) MAPSORT of: pFN21ASDA0924.seq Check: 5788 from: 1 to: 6547
pFN21ASDA0924 6547 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3535 3678 3535
Size: 143 6404
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4357 278
Size: 53 83 186 936 2821 2468
Fragments arranged by size:
2821 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3890 1937
Size: 1953 4594
Acc65I G'GTAC_C
Cuts at: 2796 3868 2796
Size: 1072 5475
AclI AA'CG_TT
Cuts at: 1563 2817 4674 5047 1563
Size: 1254 1857 373 3063
Fragments arranged by size:
3063 1857 1254 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4610 5994 6262 1632
Size: 2978 1384 268 1917
Fragments arranged by size:
2978 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2462 3473 820
Size: 197 1445 1011 3894
Fragments arranged by size:
3894 1445 1011 197
AflIII A'CryG_T
Cuts at: 1203 5446 6423 1203
Size: 4243 977 1327
Fragments arranged by size:
4243 1327 977
AgeI A'CCGG_T
Cuts at: 5378 5378
Size: 6547
AhdI GACnn_n'nnGTC
Cuts at: 5276 5276
Size: 6547
AleI CACnn'nnGTG
Cuts at: 1166 2168 1166
Size: 1002 5545
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6547
AlwNI CAG_nnn'CTG
Cuts at: 2053 2285 4290 5862 2053
Size: 232 2005 1572 2738
Fragments arranged by size:
2738 2005 1572 232
ApaLI G'TGCA_C
Cuts at: 3143 4604 5760 3143
Size: 1461 1156 3930
Fragments arranged by size:
3930 1461 1156
ApoI r'AATT_y
Cuts at: 1493 2317 3005 3018 3032 3856 4082 4234 6343
Size: 824 688 13 14 824 226 152 2109
Cuts at: 6343 1493
Size: 1697
Fragments arranged by size:
2109 1697 824 824 688 226 152 14
13
AseI AT'TA_AT
Cuts at: 160 1032 5101 160
Size: 872 4069 1606
Fragments arranged by size:
4069 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6547
AvaI C'yCGr_G
Cuts at: 1870 1943 3872 1870
Size: 73 1929 4545
Fragments arranged by size:
4545 1929 73
BamHI G'GATC_C
Cuts at: 3877 4244 3877
Size: 367 6180
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2138 2796 3804 3868 5323
Size: 325 217 627 351 658 1008 64 1455
Cuts at: 5323 618
Size: 1842
Fragments arranged by size:
1842 1455 1008 658 627 351 325 217
64
BanII G_rGCy'C
Cuts at: 1391 2712 3866 1391
Size: 1321 1154 4072
Fragments arranged by size:
4072 1321 1154
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2313 928
Size: 971 414 5162
Fragments arranged by size:
5162 971 414
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4738 4772 1903
Size: 34 2801 34 3678
Fragments arranged by size:
3678 2801 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4439 5649 4439
Size: 1210 5337
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6547
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5158 136
Size: 107 122 71 631 605 3486 1525
Fragments arranged by size:
3486 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6542 6542
Size: 6547
BlpI GC'TnA_GC
Cuts at: 3964 3964
Size: 6547
Bme1580I G_kGCm'C
Cuts at: 1790 2774 3147 3252 4608 5764 1790
Size: 984 373 105 1356 1156 2573
Fragments arranged by size:
2573 1356 1156 984 373 105
BmgBI CAC'GTC
Cuts at: 1318 2290 1318
Size: 972 5575
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3560 4278 5236
Size: 416 53 83 399 24 2143 718 958
Cuts at: 5236 442
Size: 1753
Fragments arranged by size:
2143 1753 958 718 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6547
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2822 3183 3351 3702 3726 5207 1239
Size: 1583 361 168 351 24 1481 2579
Fragments arranged by size:
2579 1583 1481 361 351 168 24
Bpu10I CC'TnA_GC
Cuts at: 2682 2682
Size: 6547
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3122 4672 5537 5835 3122
Size: 1550 865 298 3834
Fragments arranged by size:
3834 1550 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5210 882
Size: 617 3711 2219
Fragments arranged by size:
3711 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3844 4338 493
Size: 711 2640 494 2702
Fragments arranged by size:
2702 2640 711 494
BsaBI GATnn'nnATC
Cuts at: 6541 6541
Size: 6547
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4354 4736 5371 275
Size: 53 83 186 936 2821 382 635 1451
Fragments arranged by size:
2821 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4980 5378 5652 5799 6210 4980
Size: 398 274 147 411 5317
Fragments arranged by size:
5317 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2220 2250 1178
Size: 30 165 30 817 30 5475
Fragments arranged by size:
5475 817 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2348 2492 1186
Size: 1162 144 5241
Fragments arranged by size:
5241 1162 144
BseYI C'CCAG_C
Cuts at: 3556 5750 6246 3556
Size: 2194 496 3857
Fragments arranged by size:
3857 2194 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3656 1163
Size: 573 1920 4054
Fragments arranged by size:
4054 1920 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3997 4758 5786 1540
Size: 275 186 1996 761 1028 2301
Fragments arranged by size:
2301 1996 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3147 4608 4693 5764 3147
Size: 1461 85 1071 3930
Fragments arranged by size:
3930 1461 1071 85
BsmI GAATG_Cn'
Cuts at: 3157 3237 4058 4151 3157
Size: 80 821 93 5553
Fragments arranged by size:
5553 821 93 80
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2712 2774 3147 3252 3866 4608 4693
Size: 399 922 62 373 105 614 742 85
Cuts at: 4693 5764 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 922 742 614 399 373 105
85 62
BspEI T'CCGG_A
Cuts at: 6210 6210
Size: 6547
BspHI T'CATG_A
Cuts at: 2965 4436 6166 2965
Size: 1471 1730 3346
Fragments arranged by size:
3346 1730 1471
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3535 3678 3902 844
Size: 2691 143 224 3489
Fragments arranged by size:
3489 2691 224 143
BsrBI CCG'CTC
Cuts at: 1394 1823 4434 1394
Size: 429 2611 3507
Fragments arranged by size:
3507 2611 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2527 5042 5216 65
Size: 1183 1279 2515 174 1396
Fragments arranged by size:
2515 1396 1279 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5191 5378 1700
Size: 110 3381 187 2869
Fragments arranged by size:
3381 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2172 96
Size: 2076 4471
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6547
BssSI C'ACGA_G
Cuts at: 4607 5619 4607
Size: 1012 5535
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3151 794
Size: 2357 4190
BstBI TT'CG_AA
Cuts at: 2007 5351 2007
Size: 3344 3203
BstEII G'GTnAC_C
Cuts at: 1184 3502 1184
Size: 2318 4229
BstXI CCAn_nnnn'nTGG
Cuts at: 3275 3275
Size: 6547
BstYI r'GATC_y
Cuts at: 1972 3537 3877 4244 4629 4646 6098 6184 6196
Size: 1565 340 367 385 17 1452 86 12
Cuts at: 6196 6542 1972
Size: 346 1977
Fragments arranged by size:
1977 1565 1452 385 367 346 340 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2986 3042 513
Size: 552 1921 56 4018
Fragments arranged by size:
4018 1921 552 56
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5808
Fragments arranged by size:
5808 683 56
BtsI GCAGTG_nn'
Cuts at: 2286 2479 4063 4857 4877 2286
Size: 193 1584 794 20 3956
Fragments arranged by size:
3956 1584 794 193 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6547
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6512
DraI TTT'AAA
Cuts at: 3768 3851 3768
Size: 83 6464
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5554 809
Size: 507 4238 1802
Fragments arranged by size:
4238 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3445 3994 4883
Size: 54 1425 50 275 18 1615 549 889
Cuts at: 4883 6512 8
Size: 1629 43
Fragments arranged by size:
1629 1615 1425 889 549 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3994 1537
Size: 275 2182 4090
Fragments arranged by size:
4090 2182 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2334 2440 2478 2484 2496 2660 2736 3386
Size: 981 106 38 6 12 164 76 650
Cuts at: 3386 3639 4477 1353
Size: 253 838 3423
Fragments arranged by size:
3423 981 838 650 253 164 106 76
38 12 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5134 5508 5654 387
Size: 1064 138 3545 374 146 1280
Fragments arranged by size:
3545 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2822 3183 3351 3702 3726 4610 5207
Size: 393 1190 361 168 351 24 884 597
Cuts at: 5207 5994 6262 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1190 884 787 597 393 361 351
268 168 24
EcoRI G'AATT_C
Cuts at: 2317 3856 4234 2317
Size: 1539 378 4630
Fragments arranged by size:
4630 1539 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3319 3319
Size: 6547
FspI TGC'GCA
Cuts at: 5053 5053
Size: 6547
HaeII r_GCGC'y
Cuts at: 1678 5694 1678
Size: 4016 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5188 5220 5262 5294 1373
Size: 32 135 32 3616 32 42 32 2626
Fragments arranged by size:
3616 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3891 4137 669
Size: 988 281 1953 246 3079
Fragments arranged by size:
3079 1953 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3367 748
Size: 2619 3928
HpaI GTT'AAC
Cuts at: 4137 4137
Size: 6547
KpnI G_GTAC'C
Cuts at: 2800 3872 2800
Size: 1072 5475
MfeI C'AATT_G
Cuts at: 4146 4146
Size: 6547
MluI A'CGCG_T
Cuts at: 6423 6423
Size: 6547
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5661 5845 1305
Size: 4356 184 2007
Fragments arranged by size:
4356 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3447 10
Size: 54 1425 343 1615 3110
Fragments arranged by size:
3110 1615 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2168 2219 2238 2991 3094 4505 4864
Size: 648 1002 51 19 753 103 1411 359
Cuts at: 4864 5023 518
Size: 159 2042
Fragments arranged by size:
2042 1411 1002 753 648 359 159 103
51 19
MspA1I CmG'CkG
Cuts at: 1606 3451 3963 4640 5788 6033 1606
Size: 1845 512 677 1148 245 2120
Fragments arranged by size:
2120 1845 1148 677 512 245
NcoI C'CATG_G
Cuts at: 513 1065 2986 3042 513
Size: 552 1921 56 4018
Fragments arranged by size:
4018 1921 552 56
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6547
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6547
NotI GC'GGCC_GC
Cuts at: 3994 3994
Size: 6547
NspI r_CATG'y
Cuts at: 1132 1229 2078 2297 2836 3088 3099 3472 3905
Size: 97 849 219 539 252 11 373 433
Cuts at: 3905 5450 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 849 539 433 373 252 219
97 11
PasI CC'CwG_GG
Cuts at: 1348 2775 1348
Size: 1427 5120
PciI A'CATG_T
Cuts at: 5446 5446
Size: 6547
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6547
PfoI T'CCnGG_A
Cuts at: 2868 2868
Size: 6547
PmeI GTTT'AAAC
Cuts at: 3851 3851
Size: 6547
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4616 4648 6129 6161 4616
Size: 32 1481 32 5002
Fragments arranged by size:
5002 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6547
PsiI TTA'TAA
Cuts at: 4117 4117
Size: 6547
PstI C_TGCA'G
Cuts at: 830 3899 830
Size: 3069 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6547
SalI G'TCGA_C
Cuts at: 1936 3889 1936
Size: 1953 4594
SbfI CC_TGCA'GG
Cuts at: 3899 3899
Size: 6547
ScaI AGT'ACT
Cuts at: 3302 4795 6507 3302
Size: 1493 1712 3342
Fragments arranged by size:
3342 1712 1493
SfcI C'TryA_G
Cuts at: 826 1045 3895 5030 5711 5902 826
Size: 219 2850 1135 681 191 1471
Fragments arranged by size:
2850 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6547
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6547
SmaI CCC'GGG
Cuts at: 1872 3874 1872
Size: 2002 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2462 3137 3473 4651 5552 5814
Size: 197 926 519 675 336 1178 901 262
Cuts at: 5814 820
Size: 1553
Fragments arranged by size:
1553 1178 926 901 675 519 336 262
197
SnaBI TAC'GTA
Cuts at: 493 3844 4338 493
Size: 3351 494 2702
Fragments arranged by size:
3351 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6547
SphI G_CATG'C
Cuts at: 1132 2297 2836 3905 1132
Size: 1165 539 1069 3774
Fragments arranged by size:
3774 1165 1069 539
SspI AAT'ATT
Cuts at: 5 52 4471 5
Size: 47 4419 2081
Fragments arranged by size:
4419 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6547
StyI C'CwwG_G
Cuts at: 513 1065 2986 3042 3986 513
Size: 552 1921 56 944 3074
Fragments arranged by size:
3074 1921 944 552 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3696 3711 4574 4591 4744 4929 5374
Size: 1255 2116 15 863 17 153 185 445
Cuts at: 5374 325
Size: 1498
Fragments arranged by size:
2116 1498 1255 863 445 185 153 17
15
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2172 3207 3300
Size: 274 80 33 51 1443 195 1035 93
Cuts at: 3300 4793 6505 96
Size: 1493 1712 138
Fragments arranged by size:
1712 1493 1443 1035 274 195 138 93
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3516 3548 3553 3585 6507 6539 3516
Size: 32 5 32 2922 32 3524
Fragments arranged by size:
3524 2922 32 32 32 5
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6547
XbaI T'CTAG_A
Cuts at: 3883 3883
Size: 6547
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6547
XmaI C'CCGG_G
Cuts at: 1870 3872 1870
Size: 2002 4545
XmnI GAAnn'nnTTC
Cuts at: 3032 3201 4676 3032
Size: 169 1475 4903
Fragments arranged by size:
4903 1475 169
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4355 276
Size: 53 83 186 936 2821 2468
Fragments arranged by size:
2821 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI