Restriction map for
pFN21ASDA0923
(Circular) (Six-base) MAPSORT of: pFN21ASDA0923.seq Check: 9093 from: 1 to: 6571
pFN21ASDA0923 6571 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4381 278
Size: 53 83 186 936 2845 2468
Fragments arranged by size:
2845 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2656 3914 1937
Size: 719 1258 4594
Fragments arranged by size:
4594 1258 719
Acc65I G'GTAC_C
Cuts at: 3892 3892
Size: 6571
AclI AA'CG_TT
Cuts at: 1563 4698 5071 1563
Size: 3135 373 3063
Fragments arranged by size:
3135 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2046 2333 2471 2666 2784 3636 3741 4634
Size: 414 287 138 195 118 852 105 893
Cuts at: 4634 6018 6286 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 893 852 414 287 268 195
138 118 105
AflII C'TTAA_G
Cuts at: 820 1017 2444 2852 820
Size: 197 1427 408 4539
Fragments arranged by size:
4539 1427 408 197
AflIII A'CryG_T
Cuts at: 1203 3668 5470 6447 1203
Size: 2465 1802 977 1327
Fragments arranged by size:
2465 1802 1327 977
AgeI A'CCGG_T
Cuts at: 5402 5402
Size: 6571
AhdI GACnn_n'nnGTC
Cuts at: 5300 5300
Size: 6571
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6571
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6571
AlwNI CAG_nnn'CTG
Cuts at: 2948 4314 5886 2948
Size: 1366 1572 3633
Fragments arranged by size:
3633 1572 1366
ApaLI G'TGCA_C
Cuts at: 2168 3551 4628 5784 2168
Size: 1383 1077 1156 2955
Fragments arranged by size:
2955 1383 1156 1077
ApoI r'AATT_y
Cuts at: 1493 2392 2524 2793 3063 3189 3355 3835 3880
Size: 899 132 269 270 126 166 480 45
Cuts at: 3880 4106 4258 6367 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 899 480 270 269 226 166
152 132 126 45
AseI AT'TA_AT
Cuts at: 160 1032 5125 160
Size: 872 4093 1606
Fragments arranged by size:
4093 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6571
AvaI C'yCGr_G
Cuts at: 1870 1943 3179 3556 3896 1870
Size: 73 1236 377 340 4545
Fragments arranged by size:
4545 1236 377 340 73
BamHI G'GATC_C
Cuts at: 3901 4268 3901
Size: 367 6204
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3892 5347 618
Size: 325 217 627 2105 1455 1842
Fragments arranged by size:
2105 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3890 1391
Size: 2499 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2052 928
Size: 971 153 5447
Fragments arranged by size:
5447 971 153
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2149 2183 4762 4796 1903
Size: 34 212 34 2579 34 3678
Fragments arranged by size:
3678 2579 212 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4463 5673 4463
Size: 1210 5361
BclI T'GATC_A
Cuts at: 1548 2230 1548
Size: 682 5889
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5182 136
Size: 107 122 71 631 605 3510 1525
Fragments arranged by size:
3510 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6566 6566
Size: 6571
BlpI GC'TnA_GC
Cuts at: 3988 3988
Size: 6571
Bme1580I G_kGCm'C
Cuts at: 1790 2172 3555 4632 5788 1790
Size: 382 1383 1077 1156 2573
Fragments arranged by size:
2573 1383 1156 1077 382
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6571
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4302 5260 442
Size: 416 53 83 399 24 2885 958 1753
Fragments arranged by size:
2885 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6571
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2449 2767 3585 5231 1239
Size: 1210 318 818 1646 2579
Fragments arranged by size:
2579 1646 1210 818 318
Bpu10I CC'TnA_GC
Cuts at: 2370 2370
Size: 6571
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3844 4696 5561 5859 3844
Size: 852 865 298 4556
Fragments arranged by size:
4556 865 852 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3603 5234 882
Size: 617 2104 1631 2219
Fragments arranged by size:
2219 2104 1631 617
BsaAI yAC'GTr
Cuts at: 493 1204 3868 4362 493
Size: 711 2664 494 2702
Fragments arranged by size:
2702 2664 711 494
BsaBI GATnn'nnATC
Cuts at: 6565 6565
Size: 6571
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4378 4760 5395 275
Size: 53 83 186 936 2845 382 635 1451
Fragments arranged by size:
2845 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5004 5402 5676 5823 6234 5004
Size: 398 274 147 411 5341
Fragments arranged by size:
5341 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2740 2770 1178
Size: 30 165 30 1337 30 4979
Fragments arranged by size:
4979 1337 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3762 1186
Size: 2576 3995
BseYI C'CCAG_C
Cuts at: 2401 5774 6270 2401
Size: 3373 496 2702
Fragments arranged by size:
3373 2702 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2176 1163
Size: 573 440 5558
Fragments arranged by size:
5558 573 440
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4021 4782 5810 1540
Size: 275 186 2020 761 1028 2301
Fragments arranged by size:
2301 2020 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2172 3555 4632 4717 5788 2172
Size: 1383 1077 85 1071 2955
Fragments arranged by size:
2955 1383 1077 1071 85
BsmI GAATG_Cn'
Cuts at: 4082 4175 4082
Size: 93 6478
BsmBI CGTCTCn'nnnn_
Cuts at: 3320 3364 3320
Size: 44 6527
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2172 3555 3890 4632 4717 5788 1391
Size: 399 382 1383 335 742 85 1071 2174
Fragments arranged by size:
2174 1383 1071 742 399 382 335 85
BspEI T'CCGG_A
Cuts at: 6234 6234
Size: 6571
BspHI T'CATG_A
Cuts at: 2608 4460 6190 2608
Size: 1852 1730 2989
Fragments arranged by size:
2989 1852 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3926 844
Size: 3082 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4458 1394
Size: 429 2635 3507
Fragments arranged by size:
3507 2635 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2580 5066 5240 65
Size: 1183 1332 2486 174 1396
Fragments arranged by size:
2486 1396 1332 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5215 5402 1700
Size: 110 3405 187 2869
Fragments arranged by size:
3405 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3755 96
Size: 3659 2912
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6571
BssSI C'ACGA_G
Cuts at: 4631 5643 4631
Size: 1012 5559
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6571
BstBI TT'CG_AA
Cuts at: 2007 5375 2007
Size: 3368 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6571
BstYI r'GATC_y
Cuts at: 1972 2047 3901 4268 4653 4670 6122 6208 6220
Size: 75 1854 367 385 17 1452 86 12
Cuts at: 6220 6566 1972
Size: 346 1977
Fragments arranged by size:
1977 1854 1452 385 367 346 86 75
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5062
Fragments arranged by size:
5062 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5832
Fragments arranged by size:
5832 683 56
BtsI GCAGTG_nn'
Cuts at: 3203 4087 4881 4901 3203
Size: 884 794 20 4873
Fragments arranged by size:
4873 884 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6571
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6536
DraI TTT'AAA
Cuts at: 3340 3875 3340
Size: 535 6036
DraIII CAC_nnn'GTG
Cuts at: 3586 3586
Size: 6571
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5578 809
Size: 507 4262 1802
Fragments arranged by size:
4262 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4018 4907 6536
Size: 54 1425 50 275 18 2188 889 1629
Cuts at: 6536 8
Size: 43
Fragments arranged by size:
2188 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4018 1537
Size: 275 2206 4090
Fragments arranged by size:
4090 2206 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2612 2898 3636 4501 1353
Size: 1259 286 738 865 3423
Fragments arranged by size:
3423 1259 865 738 286
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5158 5532 5678 387
Size: 1064 138 3569 374 146 1280
Fragments arranged by size:
3569 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2046 2333 2449 2471 2666 2767 2784
Size: 393 414 287 116 22 195 101 17
Cuts at: 2784 3585 3636 3741 4634 5231 6018 6286 1239
Size: 801 51 105 893 597 787 268 1524
Fragments arranged by size:
1524 893 801 787 597 414 393 287
268 195 116 105 101 51 22 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2467 2467
Size: 6571
EcoO109I rG'GnC_Cy
Cuts at: 3004 3004
Size: 6571
EcoRI G'AATT_C
Cuts at: 3880 4258 3880
Size: 378 6193
FspI TGC'GCA
Cuts at: 5077 5077
Size: 6571
HaeII r_GCGC'y
Cuts at: 1678 5718 1678
Size: 4040 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3128 3160 3202 3234 5212
Size: 32 135 32 1556 32 42 32 1978
Cuts at: 5212 5244 5286 5318 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1978 1556 135 42 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3915 4161 669
Size: 988 281 1977 246 3079
Fragments arranged by size:
3079 1977 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3086 3376 748
Size: 2338 290 3943
Fragments arranged by size:
3943 2338 290
HpaI GTT'AAC
Cuts at: 4161 4161
Size: 6571
KpnI G_GTAC'C
Cuts at: 3896 3896
Size: 6571
MfeI C'AATT_G
Cuts at: 4170 4170
Size: 6571
MluI A'CGCG_T
Cuts at: 6447 6447
Size: 6571
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3279 3724 5685 5869 1305
Size: 1974 445 1961 184 2007
Fragments arranged by size:
2007 1974 1961 445 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4749
Fragments arranged by size:
4749 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4529 4888 5047 518
Size: 648 3363 359 159 2042
Fragments arranged by size:
3363 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2353 3987 4664 5812 6057 1606
Size: 747 1634 677 1148 245 2120
Fragments arranged by size:
2120 1634 1148 747 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5062
Fragments arranged by size:
5062 957 552
NdeI CA'TA_TG
Cuts at: 387 3256 387
Size: 2869 3702
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6571
NotI GC'GGCC_GC
Cuts at: 4018 4018
Size: 6571
NspI r_CATG'y
Cuts at: 1132 1229 3613 3929 5474 1132
Size: 97 2384 316 1545 2229
Fragments arranged by size:
2384 2229 1545 316 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6571
PciI A'CATG_T
Cuts at: 5470 5470
Size: 6571
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6571
PfoI T'CCnGG_A
Cuts at: 2465 2744 2465
Size: 279 6292
PmeI GTTT'AAAC
Cuts at: 3875 3875
Size: 6571
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4640 4672 6153 6185 4640
Size: 32 1481 32 5026
Fragments arranged by size:
5026 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3004 3004
Size: 6571
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6571
PsiI TTA'TAA
Cuts at: 4141 4141
Size: 6571
PspXI vC'TCGA_Gb
Cuts at: 3179 3556 3179
Size: 377 6194
PstI C_TGCA'G
Cuts at: 830 3923 830
Size: 3093 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6571
SalI G'TCGA_C
Cuts at: 1936 3913 1936
Size: 1977 4594
SbfI CC_TGCA'GG
Cuts at: 3923 3923
Size: 6571
ScaI AGT'ACT
Cuts at: 4819 6531 4819
Size: 1712 4859
SfcI C'TryA_G
Cuts at: 826 1045 2423 3808 3919 5054 5735 5926 826
Size: 219 1378 1385 111 1135 681 191 1471
Fragments arranged by size:
1471 1385 1378 1135 681 219 191 111
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6571
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6571
SmaI CCC'GGG
Cuts at: 1872 3898 1872
Size: 2026 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2444 2852 3179 3556 3823 4675
Size: 197 926 501 408 327 377 267 852
Cuts at: 4675 5576 5838 820
Size: 901 262 1553
Fragments arranged by size:
1553 926 901 852 501 408 377 327
267 262 197
SnaBI TAC'GTA
Cuts at: 493 3868 4362 493
Size: 3375 494 2702
Fragments arranged by size:
3375 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6571
SphI G_CATG'C
Cuts at: 1132 3929 1132
Size: 2797 3774
SspI AAT'ATT
Cuts at: 5 52 2206 4495 5
Size: 47 2154 2289 2081
Fragments arranged by size:
2289 2154 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6571
StyI C'CwwG_G
Cuts at: 513 1065 2022 4010 513
Size: 552 957 1988 3074
Fragments arranged by size:
3074 1988 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4598 4615 4768 4953 5398 325
Size: 1255 3018 17 153 185 445 1498
Fragments arranged by size:
3018 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2317 2939 3703
Size: 274 80 33 51 1443 340 622 764
Cuts at: 3703 3755 4817 6529 96
Size: 52 1062 1712 138
Fragments arranged by size:
1712 1443 1062 764 622 340 274 138
80 52 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6531 6563 6531
Size: 32 6539
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6571
XbaI T'CTAG_A
Cuts at: 3907 3907
Size: 6571
XhoI C'TCGA_G
Cuts at: 1943 3179 3556 1943
Size: 1236 377 4958
Fragments arranged by size:
4958 1236 377
XmaI C'CCGG_G
Cuts at: 1870 3896 1870
Size: 2026 4545
XmnI GAAnn'nnTTC
Cuts at: 3506 4700 3506
Size: 1194 5377
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4379 276
Size: 53 83 186 936 2845 2468
Fragments arranged by size:
2845 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspXI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI