Restriction map for
pFN21ASDA0899
(Circular) (Six-base) MAPSORT of: pFN21ASDA0899.seq Check: 4306 from: 1 to: 7546
pFN21ASDA0899 7546 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2613 3600 4360 4369 2613
Size: 987 760 9 5790
Fragments arranged by size:
5790 987 760 9
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2558 2738 5356 278
Size: 53 83 186 936 1022 180 2618 2468
Fragments arranged by size:
2618 2468 1022 936 186 180 83 53
AccI GT'mk_AC
Cuts at: 1937 3980 4889 1937
Size: 2043 909 4594
Fragments arranged by size:
4594 2043 909
Acc65I G'GTAC_C
Cuts at: 3405 4867 3405
Size: 1462 6084
AclI AA'CG_TT
Cuts at: 1563 4540 5673 6046 1563
Size: 2977 1133 373 3063
Fragments arranged by size:
3063 2977 1133 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3170 3317 3464 4295 5609 6993 7261 1632
Size: 1538 147 147 831 1314 1384 268 1917
Fragments arranged by size:
1917 1538 1384 1314 831 268 147 147
AflII C'TTAA_G
Cuts at: 820 1017 4202 820
Size: 197 3185 4164
Fragments arranged by size:
4164 3185 197
AflIII A'CryG_T
Cuts at: 1203 4080 4464 6445 7422 1203
Size: 2877 384 1981 977 1327
Fragments arranged by size:
2877 1981 1327 977 384
AgeI A'CCGG_T
Cuts at: 6377 6377
Size: 7546
AhdI GACnn_n'nnGTC
Cuts at: 6275 6275
Size: 7546
AleI CACnn'nnGTG
Cuts at: 1166 2625 1166
Size: 1459 6087
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3771 4085 1184
Size: 2587 314 4645
Fragments arranged by size:
4645 2587 314
AlwNI CAG_nnn'CTG
Cuts at: 2840 3031 5289 6861 2840
Size: 191 2258 1572 3525
Fragments arranged by size:
3525 2258 1572 191
ApaI G_GGCC'C
Cuts at: 3884 4043 3884
Size: 159 7387
ApaLI G'TGCA_C
Cuts at: 2600 3079 5603 6759 2600
Size: 479 2524 1156 3387
Fragments arranged by size:
3387 2524 1156 479
ApoI r'AATT_y
Cuts at: 1493 2150 4211 4222 4496 4681 4855 5081 5233
Size: 657 2061 11 274 185 174 226 152
Cuts at: 5233 7342 1493
Size: 2109 1697
Fragments arranged by size:
2109 2061 1697 657 274 226 185 174
152 11
AseI AT'TA_AT
Cuts at: 160 1032 6100 160
Size: 872 5068 1606
Fragments arranged by size:
5068 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7546
AvaI C'yCGr_G
Cuts at: 1870 1943 2592 3562 4013 4871 1870
Size: 73 649 970 451 858 4545
Fragments arranged by size:
4545 970 858 649 451 73
BamHI G'GATC_C
Cuts at: 4876 5243 4876
Size: 367 7179
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3405 4417 4451 4867 6322
Size: 325 217 627 1618 1012 34 416 1455
Cuts at: 6322 618
Size: 1842
Fragments arranged by size:
1842 1618 1455 1012 627 416 325 217
34
BanII G_rGCy'C
Cuts at: 1391 3884 4043 4865 1391
Size: 2493 159 822 4072
Fragments arranged by size:
4072 2493 822 159
BbeI G_GCGC'C
Cuts at: 4421 4421
Size: 7546
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2059 3158 4137 928
Size: 971 160 1099 979 4337
Fragments arranged by size:
4337 1099 979 971 160
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5737 5771 1903
Size: 34 3800 34 3678
Fragments arranged by size:
3800 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3343 5438 6648 3343
Size: 2095 1210 4241
Fragments arranged by size:
4241 2095 1210
BclI T'GATC_A
Cuts at: 1548 2355 2652 1548
Size: 807 297 6442
Fragments arranged by size:
6442 807 297
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3311 6157 136
Size: 107 122 71 631 605 1639 2846 1525
Fragments arranged by size:
2846 1639 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2749 7541 2749
Size: 4792 2754
BlpI GC'TnA_GC
Cuts at: 2720 3248 3776 4963 2720
Size: 528 528 1187 5303
Fragments arranged by size:
5303 1187 528 528
Bme1580I G_kGCm'C
Cuts at: 1790 2604 3083 3884 4043 4454 5607 6763 1790
Size: 814 479 801 159 411 1153 1156 2573
Fragments arranged by size:
2573 1156 1153 814 801 479 411 159
BmgBI CAC'GTC
Cuts at: 1318 3166 3442 3703 3739 3934 4081 1318
Size: 1848 276 261 36 195 147 4783
Fragments arranged by size:
4783 1848 276 261 195 147 36
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5277 6235 442
Size: 416 53 83 399 24 3860 958 1753
Fragments arranged by size:
3860 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7546
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2738 2891 3089 3278 3576 3836 3923 6206
Size: 1499 153 198 189 298 260 87 2283
Cuts at: 6206 1239
Size: 2579
Fragments arranged by size:
2579 2283 1499 298 260 198 189 153
87
Bpu10I CC'TnA_GC
Cuts at: 3725 3948 3725
Size: 223 7323
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3851 5671 6536 6834 3851
Size: 1820 865 298 4563
Fragments arranged by size:
4563 1820 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2867 3038 3552 4016 6209 882
Size: 617 1368 171 514 464 2193 2219
Fragments arranged by size:
2219 2193 1368 617 514 464 171
BsaAI yAC'GTr
Cuts at: 493 1204 3388 4843 5337 493
Size: 711 2184 1455 494 2702
Fragments arranged by size:
2702 2184 1455 711 494
BsaBI GATnn'nnATC
Cuts at: 3425 4238 4671 7540 3425
Size: 813 433 2869 3431
Fragments arranged by size:
3431 2869 813 433
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2555 2735 4418 5353
Size: 53 83 186 936 1022 180 1683 935
Cuts at: 5353 5735 6370 275
Size: 382 635 1451
Fragments arranged by size:
1683 1451 1022 936 935 635 382 186
180 83 53
BsaWI w'CCGG_w
Cuts at: 5979 6377 6651 6798 7209 5979
Size: 398 274 147 411 6316
Fragments arranged by size:
6316 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3062 3092 3830 3860 3973
Size: 30 165 30 1659 30 738 30 113
Cuts at: 3973 4003 1178
Size: 30 4721
Fragments arranged by size:
4721 1659 738 165 113 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3185 3833 4042 1186
Size: 1999 648 209 4690
Fragments arranged by size:
4690 1999 648 209
BseYI C'CCAG_C
Cuts at: 3884 3958 4006 6749 7245 3884
Size: 74 48 2743 496 4185
Fragments arranged by size:
4185 2743 496 74 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2402 3464 3581 3637 4394 4493 4595
Size: 573 666 1062 117 56 757 99 102
Cuts at: 4595 4632 1163
Size: 37 4077
Fragments arranged by size:
4077 1062 757 666 573 117 102 99
56 37
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2031 3214 3810 4353 4996 5757
Size: 275 186 30 1183 596 543 643 761
Cuts at: 5757 6785 1540
Size: 1028 2301
Fragments arranged by size:
2301 1183 1028 761 643 596 543 275
186 30
BsiHKAI G_wGCw'C
Cuts at: 2604 3083 5607 5692 6763 2604
Size: 479 2524 85 1071 3387
Fragments arranged by size:
3387 2524 1071 479 85
BsmI GAATG_Cn'
Cuts at: 2944 5057 5150 2944
Size: 2113 93 5340
Fragments arranged by size:
5340 2113 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3125 3125
Size: 7546
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2604 3083 3884 4043 4454 4865 5607
Size: 399 814 479 801 159 411 411 742
Cuts at: 5607 5692 6763 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 814 801 742 479 411 411
399 159 85
BspEI T'CCGG_A
Cuts at: 7209 7209
Size: 7546
BspHI T'CATG_A
Cuts at: 2230 5435 7165 2230
Size: 3205 1730 2611
Fragments arranged by size:
3205 2611 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2274 2613 3003 3060 3600 3777 4011 4335
Size: 1430 339 390 57 540 177 234 324
Cuts at: 4335 4360 4369 4761 4901 844
Size: 25 9 392 140 3489
Fragments arranged by size:
3489 1430 540 392 390 339 324 234
177 140 57 25 9
BsrBI CCG'CTC
Cuts at: 1394 1823 3160 3841 5433 1394
Size: 429 1337 681 1592 3507
Fragments arranged by size:
3507 1592 1337 681 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6041 6215 65
Size: 1183 4793 174 1396
Fragments arranged by size:
4793 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2026 4770 6190 6377 1700
Size: 110 216 2744 1420 187 2869
Fragments arranged by size:
2869 2744 1420 216 187 110
BsrGI T'GTAC_A
Cuts at: 96 2547 96
Size: 2451 5095
BssHII G'CGCG_C
Cuts at: 1925 3633 4365 1925
Size: 1708 732 5106
Fragments arranged by size:
5106 1708 732
BssSI C'ACGA_G
Cuts at: 3497 4073 5606 6618 3497
Size: 576 1533 1012 4425
Fragments arranged by size:
4425 1533 1012 576
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4573 794
Size: 3779 3767
BstBI TT'CG_AA
Cuts at: 2007 6350 2007
Size: 4343 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7546
BstXI CCAn_nnnn'nTGG
Cuts at: 2404 4342 2404
Size: 1938 5608
BstYI r'GATC_y
Cuts at: 1972 2472 2749 3566 4876 5243 5628 5645 7097
Size: 500 277 817 1310 367 385 17 1452
Cuts at: 7097 7183 7195 7541 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1310 817 500 385 367 346
277 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2478 2478
Size: 7546
BtgI C'CryG_G
Cuts at: 513 1065 2574 3186 3783 4035 4341 4353 513
Size: 552 1509 612 597 252 306 12 3706
Fragments arranged by size:
3706 1509 612 597 552 306 252 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2410 487
Size: 683 56 1184 5623
Fragments arranged by size:
5623 1184 683 56
BtsI GCAGTG_nn'
Cuts at: 2419 2825 2850 5062 5856 5876 2419
Size: 406 25 2212 794 20 4089
Fragments arranged by size:
4089 2212 794 406 25 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7546
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4705 4740 529
Size: 35 4141 35 3335
Fragments arranged by size:
4141 3335 35 35
DraI TTT'AAA
Cuts at: 2155 2689 4850 2155
Size: 534 2161 4851
Fragments arranged by size:
4851 2161 534
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4613 6553 809
Size: 507 3297 1940 1802
Fragments arranged by size:
3297 1940 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 2382 2451
Size: 54 1425 50 275 18 198 354 69
Cuts at: 2451 3087 3237 3456 3786 4993 5882 7511 8
Size: 636 150 219 330 1207 889 1629 43
Fragments arranged by size:
1629 1425 1207 889 636 354 330 275
219 198 150 69 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2028 4993 1537
Size: 275 216 2965 4090
Fragments arranged by size:
4090 2965 275 216
EarI CTCTTCn'nnn_
Cuts at: 1353 2216 2676 3279 3390 3483 3677 5476 1353
Size: 863 460 603 111 93 194 1799 3423
Fragments arranged by size:
3423 1799 863 603 460 194 111 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2339 6133 6507 6653 387
Size: 1064 138 750 3794 374 146 1280
Fragments arranged by size:
3794 1280 1064 750 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2738 2891 3089 3170 3278 3317 3464
Size: 393 1106 153 198 81 108 39 147
Cuts at: 3464 3576 3836 3923 4295 5609 6206 6993 7261
Size: 112 260 87 372 1314 597 787 268
Cuts at: 7261 1239
Size: 1524
Fragments arranged by size:
1524 1314 1106 787 597 393 372 268
260 198 153 147 112 108 87 81
39
EcoO109I rG'GnC_Cy
Cuts at: 2056 2482 3880 3881 3944 4040 2056
Size: 426 1398 1 63 96 5562
Fragments arranged by size:
5562 1398 426 96 63 1
EcoRI G'AATT_C
Cuts at: 4855 5233 4855
Size: 378 7168
EcoRV GAT'ATC
Cuts at: 2080 2080
Size: 7546
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2227 2227
Size: 7546
FspI TGC'GCA
Cuts at: 3710 4784 6052 3710
Size: 1074 1268 5204
Fragments arranged by size:
5204 1268 1074
HaeII r_GCGC'y
Cuts at: 1678 3975 4421 6693 1678
Size: 2297 446 2272 2531
Fragments arranged by size:
2531 2297 2272 446
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2573 2605 3551 3583 3830
Size: 32 135 32 1001 32 946 32 247
Cuts at: 3830 3862 4070 4082 4102 4114 4385 4417 4683
Size: 32 208 12 20 12 271 32 266
Cuts at: 4683 4715 6187 6219 6261 6293 1373
Size: 32 1472 32 42 32 2626
Fragments arranged by size:
2626 1472 1001 946 271 266 247 208
135 42 32 32 32 32 32 32
32 32 32 20 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 4387 4890 5136 669
Size: 988 281 2449 503 246 3079
Fragments arranged by size:
3079 2449 988 503 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7546
HpaI GTT'AAC
Cuts at: 5136 5136
Size: 7546
KasI G'GCGC_C
Cuts at: 4417 4417
Size: 7546
KpnI G_GTAC'C
Cuts at: 3409 4871 3409
Size: 1462 6084
MfeI C'AATT_G
Cuts at: 5145 5145
Size: 7546
MluI A'CGCG_T
Cuts at: 7422 7422
Size: 7546
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2952 4211 4428 4523 6660 6844 1305
Size: 1647 1259 217 95 2137 184 2007
Fragments arranged by size:
2137 2007 1647 1259 217 184 95
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2384 3089 3788 10
Size: 54 1425 343 552 705 699 3768
Fragments arranged by size:
3768 1425 705 699 552 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2318 2402 2625 2982 5504 5863 6022
Size: 648 1152 84 223 357 2522 359 159
Cuts at: 6022 518
Size: 2042
Fragments arranged by size:
2522 2042 1152 648 359 357 223 159
84
MspA1I CmG'CkG
Cuts at: 1606 3181 3266 3577 4037 4065 4186 4477 4962
Size: 1575 85 311 460 28 121 291 485
Cuts at: 4962 5639 6787 7032 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1575 1148 677 485 460 311 291
245 121 85 28
NaeI GCC'GGC
Cuts at: 2028 2028
Size: 7546
NarI GG'CG_CC
Cuts at: 4418 4418
Size: 7546
NcoI C'CATG_G
Cuts at: 513 1065 2574 513
Size: 552 1509 5485
Fragments arranged by size:
5485 1509 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7546
NgoMIV G'CCGG_C
Cuts at: 2026 2026
Size: 7546
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7546
NotI GC'GGCC_GC
Cuts at: 4993 4993
Size: 7546
NspI r_CATG'y
Cuts at: 1132 1229 3079 4904 6449 1132
Size: 97 1850 1825 1545 2229
Fragments arranged by size:
2229 1850 1825 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7546
PciI A'CATG_T
Cuts at: 6445 6445
Size: 7546
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2623 1108
Size: 1515 6031
PfoI T'CCnGG_A
Cuts at: 2754 3675 3696 3834 2754
Size: 921 21 138 6466
Fragments arranged by size:
6466 921 138 21
PmeI GTTT'AAAC
Cuts at: 4850 4850
Size: 7546
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5615 5647 7128 7160 5615
Size: 32 1481 32 6001
Fragments arranged by size:
6001 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2482 3944 2482
Size: 1462 6084
PshAI GACnn'nnGTC
Cuts at: 1613 4204 1613
Size: 2591 4955
PsiI TTA'TAA
Cuts at: 5116 5116
Size: 7546
PspOMI G'GGCC_C
Cuts at: 3880 4039 3880
Size: 159 7387
PstI C_TGCA'G
Cuts at: 830 2641 2820 2845 3036 3750 4332 4898 830
Size: 1811 179 25 191 714 582 566 3478
Fragments arranged by size:
3478 1811 714 582 566 191 179 25
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7546
PvuII CAG'CTG
Cuts at: 3266 4186 4477 3266
Size: 920 291 6335
Fragments arranged by size:
6335 920 291
SacII CC_GC'GG
Cuts at: 4038 4038
Size: 7546
SalI G'TCGA_C
Cuts at: 1936 4888 1936
Size: 2952 4594
SapI GCTCTTCn'nnn_
Cuts at: 2216 3483 2216
Size: 1267 6279
SbfI CC_TGCA'GG
Cuts at: 4898 4898
Size: 7546
ScaI AGT'ACT
Cuts at: 5794 7506 5794
Size: 1712 5834
SexAI A'CCwGG_T
Cuts at: 3338 3504 3338
Size: 166 7380
SfcI C'TryA_G
Cuts at: 826 1045 2179 2637 2816 2841 3032 3746 4328
Size: 219 1134 458 179 25 191 714 582
Cuts at: 4328 4894 6029 6710 6901 826
Size: 566 1135 681 191 1471
Fragments arranged by size:
1471 1135 1134 714 681 582 566 458
219 191 191 179 25
SfoI GGC'GCC
Cuts at: 4419 4419
Size: 7546
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7546
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7546
SmaI CCC'GGG
Cuts at: 1872 4873 1872
Size: 3001 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3866 4202 5650 6551 6813 820
Size: 197 926 1923 336 1448 901 262 1553
Fragments arranged by size:
1923 1553 1448 926 901 336 262 197
SnaBI TAC'GTA
Cuts at: 493 4843 5337 493
Size: 4350 494 2702
Fragments arranged by size:
4350 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7546
SphI G_CATG'C
Cuts at: 1132 4904 1132
Size: 3772 3774
SspI AAT'ATT
Cuts at: 5 52 5470 5
Size: 47 5418 2081
Fragments arranged by size:
5418 2081 47
StuI AGG'CCT
Cuts at: 1517 2459 1517
Size: 942 6604
StyI C'CwwG_G
Cuts at: 513 1065 2037 2224 2574 4985 513
Size: 552 972 187 350 2411 3074
Fragments arranged by size:
3074 2411 972 552 350 187
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2615 4614 4733 5573 5590 5743 5928
Size: 1255 1035 1999 119 840 17 153 185
Cuts at: 5928 6373 325
Size: 445 1498
Fragments arranged by size:
1999 1498 1255 1035 840 445 185 153
119 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2547 5792 7504
Size: 274 80 33 51 1443 570 3245 1712
Cuts at: 7504 96
Size: 138
Fragments arranged by size:
3245 1712 1443 570 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3062 3094 7506 7538 3062
Size: 32 4412 32 3070
Fragments arranged by size:
4412 3070 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7546
XbaI T'CTAG_A
Cuts at: 4882 4882
Size: 7546
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7546
XmaI C'CCGG_G
Cuts at: 1870 4871 1870
Size: 3001 4545
XmnI GAAnn'nnTTC
Cuts at: 3671 4583 5675 3671
Size: 912 1092 5542
Fragments arranged by size:
5542 1092 912
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2556 2736 5354 276
Size: 53 83 186 936 1022 180 2618 2468
Fragments arranged by size:
2618 2468 1022 936 186 180 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BstZ17I DraIII EcoICRI EcoNI FseI FspAI NruI
NsiI PacI PmlI PspXI PsrI RsrII SacI SanDI
SfiI SrfI SwaI XcmI