Restriction map for
pFN21ASDA0893
(Circular) (Six-base) MAPSORT of: pFN21ASDA0893.seq Check: 6853 from: 1 to: 7486
pFN21ASDA0893 7486 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5296 278
Size: 53 83 186 936 3760 2468
Fragments arranged by size:
3760 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4829 1937
Size: 2892 4594
Acc65I G'GTAC_C
Cuts at: 4807 4807
Size: 7486
AclI AA'CG_TT
Cuts at: 1563 5613 5986 1563
Size: 4050 373 3063
Fragments arranged by size:
4050 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2052 2201 2303 2750 3702 5549 6933 7201
Size: 420 149 102 447 952 1847 1384 268
Cuts at: 7201 1632
Size: 1917
Fragments arranged by size:
1917 1847 1384 952 447 420 268 149
102
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7289
AflIII A'CryG_T
Cuts at: 1203 6385 7362 1203
Size: 5182 977 1327
Fragments arranged by size:
5182 1327 977
AgeI A'CCGG_T
Cuts at: 6317 6317
Size: 7486
AhdI GACnn_n'nnGTC
Cuts at: 6215 6215
Size: 7486
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7486
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7486
AlwNI CAG_nnn'CTG
Cuts at: 2350 5229 6801 2350
Size: 2879 1572 3035
Fragments arranged by size:
3035 2879 1572
ApaLI G'TGCA_C
Cuts at: 3948 5543 6699 3948
Size: 1595 1156 4735
Fragments arranged by size:
4735 1595 1156
ApoI r'AATT_y
Cuts at: 1493 2089 2249 2354 2543 2648 3012 3898 4130
Size: 596 160 105 189 105 364 886 232
Cuts at: 4130 4795 5021 5173 7282 1493
Size: 665 226 152 2109 1697
Fragments arranged by size:
2109 1697 886 665 596 364 232 226
189 160 152 105 105
AseI AT'TA_AT
Cuts at: 160 1032 3822 6040 160
Size: 872 2790 2218 1606
Fragments arranged by size:
2790 2218 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7486
AvaI C'yCGr_G
Cuts at: 1870 1943 4811 1870
Size: 73 2868 4545
Fragments arranged by size:
4545 2868 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3147 3180 3147
Size: 33 7453
BamHI G'GATC_C
Cuts at: 4007 4816 5183 4007
Size: 809 367 6310
Fragments arranged by size:
6310 809 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4807 6262 618
Size: 325 217 627 3020 1455 1842
Fragments arranged by size:
3020 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2343 4585 4805 1391
Size: 952 2242 220 4072
Fragments arranged by size:
4072 2242 952 220
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2779 2929 3840 928
Size: 971 880 150 911 4574
Fragments arranged by size:
4574 971 911 880 150
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5677 5711 1903
Size: 34 3740 34 3678
Fragments arranged by size:
3740 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5378 6588 5378
Size: 1210 6276
BclI T'GATC_A
Cuts at: 1548 3418 1548
Size: 1870 5616
BfrBI ATG'CAT
Cuts at: 4141 4141
Size: 7486
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6097 136
Size: 107 122 71 631 605 4425 1525
Fragments arranged by size:
4425 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4123 7481 4123
Size: 3358 4128
BlpI GC'TnA_GC
Cuts at: 4903 4903
Size: 7486
Bme1580I G_kGCm'C
Cuts at: 1790 3952 5547 6703 1790
Size: 2162 1595 1156 2573
Fragments arranged by size:
2573 2162 1595 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7486
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4296 4446 5217
Size: 416 53 83 399 24 2879 150 771
Cuts at: 5217 6175 442
Size: 958 1753
Fragments arranged by size:
2879 1753 958 771 416 399 150 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7486
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2943 2943
Size: 7486
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2144 2691 3707 3741 3894 4048 4233 4333
Size: 905 547 1016 34 153 154 185 100
Cuts at: 4333 4599 6146 1239
Size: 266 1547 2579
Fragments arranged by size:
2579 1547 1016 905 547 266 185 154
153 100 34
Bpu10I CC'TnA_GC
Cuts at: 3147 3963 3147
Size: 816 6670
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3128 5611 6476 6774 3128
Size: 2483 865 298 3840
Fragments arranged by size:
3840 2483 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6149 882
Size: 617 4650 2219
Fragments arranged by size:
4650 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4783 5277 493
Size: 711 3579 494 2702
Fragments arranged by size:
3579 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 2742 7480 2742
Size: 4738 2748
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5293 5675 6310 275
Size: 53 83 186 936 3760 382 635 1451
Fragments arranged by size:
3760 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5919 6317 6591 6738 7149 5919
Size: 398 274 147 411 6256
Fragments arranged by size:
6256 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3631 3661 4021 4051 1178
Size: 30 165 30 2228 30 360 30 4613
Fragments arranged by size:
4613 2228 360 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2868 3639 4206 4527 1186
Size: 1682 771 567 321 4145
Fragments arranged by size:
4145 1682 771 567 321
BseYI C'CCAG_C
Cuts at: 2343 2492 6689 7185 2343
Size: 149 4197 496 2644
Fragments arranged by size:
4197 2644 496 149
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4446 4723 1163
Size: 573 2710 277 3926
Fragments arranged by size:
3926 2710 573 277
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4936 5697 6725 1540
Size: 275 186 2935 761 1028 2301
Fragments arranged by size:
2935 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3241 3952 4585 4683 5547 5632 6703 3241
Size: 711 633 98 864 85 1071 4024
Fragments arranged by size:
4024 1071 864 711 633 98 85
BsmI GAATG_Cn'
Cuts at: 2642 4997 5090 2642
Size: 2355 93 5038
Fragments arranged by size:
5038 2355 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4466 4466
Size: 7486
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2343 3241 3952 4585 4683 4805 5547
Size: 399 553 898 711 633 98 122 742
Cuts at: 5547 5632 6703 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 898 742 711 633 553 399
122 98 85
BspEI T'CCGG_A
Cuts at: 7149 7149
Size: 7486
BspHI T'CATG_A
Cuts at: 3001 5375 7105 3001
Size: 2374 1730 3382
Fragments arranged by size:
3382 2374 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4841 844
Size: 3997 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2955 5373 1394
Size: 429 1132 2418 3507
Fragments arranged by size:
3507 2418 1132 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2384 3536 3556 5981 6155 65
Size: 1183 1136 1152 20 2425 174 1396
Fragments arranged by size:
2425 1396 1183 1152 1136 174 20
BsrFI r'CCGG_y
Cuts at: 1700 1810 3970 6130 6317 1700
Size: 110 2160 2160 187 2869
Fragments arranged by size:
2869 2160 2160 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7486
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7486
BssSI C'ACGA_G
Cuts at: 2502 5546 6558 2502
Size: 3044 1012 3430
Fragments arranged by size:
3430 3044 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7486
BstBI TT'CG_AA
Cuts at: 2007 6290 2007
Size: 4283 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7486
BstYI r'GATC_y
Cuts at: 1972 2743 3554 4007 4123 4366 4447 4720 4816
Size: 771 811 453 116 243 81 273 96
Cuts at: 4816 5183 5568 5585 7037 7123 7135 7481 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 811 771 453 385 367 346
273 243 116 96 86 81 17 12
Bsu36I CC'TnA_GG
Cuts at: 2177 2177
Size: 7486
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6934
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2336 487
Size: 683 56 1110 5637
Fragments arranged by size:
5637 1110 683 56
BtsI GCAGTG_nn'
Cuts at: 3193 3861 4429 4434 5002 5796 5816 3193
Size: 668 568 5 568 794 20 4863
Fragments arranged by size:
4863 794 668 568 568 20 5
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7486
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7451
DraI TTT'AAA
Cuts at: 3985 4790 3985
Size: 805 6681
DraIII CAC_nnn'GTG
Cuts at: 3701 3701
Size: 7486
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2997 4073 6493 809
Size: 507 1681 1076 2420 1802
Fragments arranged by size:
2420 1802 1681 1076 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4933 5822 7451
Size: 54 1425 50 275 18 3103 889 1629
Cuts at: 7451 8
Size: 43
Fragments arranged by size:
3103 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4933 1537
Size: 275 3121 4090
Fragments arranged by size:
4090 3121 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2514 3721 3780 5416 1353
Size: 1161 1207 59 1636 3423
Fragments arranged by size:
3423 1636 1207 1161 59
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6073 6447 6593 387
Size: 1064 138 4484 374 146 1280
Fragments arranged by size:
4484 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4583 4583
Size: 7486
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2052 2144 2201 2303 2691 2750 3702
Size: 393 420 92 57 102 388 59 952
Cuts at: 3702 3707 3741 3894 4048 4233 4333 4599 5549
Size: 5 34 153 154 185 100 266 950
Cuts at: 5549 6146 6933 7201 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 952 950 787 597 420 393 388
268 266 185 154 153 102 100 92
59 57 34 5
EcoNI CCTnn'n_nnAGG
Cuts at: 2126 2126
Size: 7486
EcoO109I rG'GnC_Cy
Cuts at: 4261 4642 4261
Size: 381 7105
EcoRI G'AATT_C
Cuts at: 2089 2543 4795 5173 2089
Size: 454 2252 378 4402
Fragments arranged by size:
4402 2252 454 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2645 2717 2645
Size: 72 7414
FspI TGC'GCA
Cuts at: 5992 5992
Size: 7486
HaeII r_GCGC'y
Cuts at: 1678 6633 1678
Size: 4955 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2437 2469 2941 2973 3782
Size: 32 135 32 865 32 472 32 809
Cuts at: 3782 3814 4544 4576 6127 6159 6201 6233 1373
Size: 32 730 32 1551 32 42 32 2626
Fragments arranged by size:
2626 1551 865 809 730 472 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2314 2830 4830 5076 669
Size: 988 281 376 516 2000 246 3079
Fragments arranged by size:
3079 2000 988 516 376 281 246
HindIII A'AGCT_T
Cuts at: 748 2099 2301 3467 748
Size: 1351 202 1166 4767
Fragments arranged by size:
4767 1351 1166 202
HpaI GTT'AAC
Cuts at: 2314 5076 2314
Size: 2762 4724
KpnI G_GTAC'C
Cuts at: 4811 4811
Size: 7486
MfeI C'AATT_G
Cuts at: 5085 5085
Size: 7486
MluI A'CGCG_T
Cuts at: 7362 7362
Size: 7486
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6600 6784 1305
Size: 5295 184 2007
Fragments arranged by size:
5295 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5664
Fragments arranged by size:
5664 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2111 4548 4670 5444 5803 5962 518
Size: 648 945 2437 122 774 359 159 2042
Fragments arranged by size:
2437 2042 945 774 648 359 159 122
MspA1I CmG'CkG
Cuts at: 1606 2936 3361 3872 4902 5579 6727 6972 1606
Size: 1330 425 511 1030 677 1148 245 2120
Fragments arranged by size:
2120 1330 1148 1030 677 511 425 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6934
NdeI CA'TA_TG
Cuts at: 387 2860 387
Size: 2473 5013
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7486
NotI GC'GGCC_GC
Cuts at: 4933 4933
Size: 7486
NsiI A_TGCA'T
Cuts at: 4143 4143
Size: 7486
NspI r_CATG'y
Cuts at: 1132 1229 2390 4498 4844 6389 1132
Size: 97 1161 2108 346 1545 2229
Fragments arranged by size:
2229 2108 1545 1161 346 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7486
PciI A'CATG_T
Cuts at: 6385 6385
Size: 7486
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2350 3746 1108
Size: 1242 1396 4848
Fragments arranged by size:
4848 1396 1242
PfoI T'CCnGG_A
Cuts at: 3247 3658 3247
Size: 411 7075
PmeI GTTT'AAAC
Cuts at: 4790 4790
Size: 7486
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5555 5587 7068 7100 5555
Size: 32 1481 32 5941
Fragments arranged by size:
5941 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4642 4642
Size: 7486
PshAI GACnn'nnGTC
Cuts at: 1613 3603 1613
Size: 1990 5496
PsiI TTA'TAA
Cuts at: 5056 5056
Size: 7486
PstI C_TGCA'G
Cuts at: 830 4747 4838 830
Size: 3917 91 3478
Fragments arranged by size:
3917 3478 91
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7486
PvuII CAG'CTG
Cuts at: 2936 3872 2936
Size: 936 6550
SacI G_AGCT'C
Cuts at: 4585 4585
Size: 7486
SalI G'TCGA_C
Cuts at: 1936 4828 1936
Size: 2892 4594
SbfI CC_TGCA'GG
Cuts at: 4838 4838
Size: 7486
ScaI AGT'ACT
Cuts at: 5734 7446 5734
Size: 1712 5774
SfcI C'TryA_G
Cuts at: 826 1045 2422 4437 4443 4743 4834 5969 6650
Size: 219 1377 2015 6 300 91 1135 681
Cuts at: 6650 6841 826
Size: 191 1471
Fragments arranged by size:
2015 1471 1377 1135 681 300 219 191
91 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7486
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7486
SmaI CCC'GGG
Cuts at: 1872 4813 1872
Size: 2941 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3107 5590 6491 6753 820
Size: 197 926 1164 2483 901 262 1553
Fragments arranged by size:
2483 1553 1164 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4783 5277 493
Size: 4290 494 2702
Fragments arranged by size:
4290 2702 494
SpeI A'CTAG_T
Cuts at: 152 3575 152
Size: 3423 4063
SphI G_CATG'C
Cuts at: 1132 4844 1132
Size: 3712 3774
SspI AAT'ATT
Cuts at: 5 52 2821 3308 3484 3634 5410 5
Size: 47 2769 487 176 150 1776 2081
Fragments arranged by size:
2769 2081 1776 487 176 150 47
StuI AGG'CCT
Cuts at: 1517 2625 3178 1517
Size: 1108 553 5825
Fragments arranged by size:
5825 1108 553
StyI C'CwwG_G
Cuts at: 513 1065 3022 3499 4925 513
Size: 552 1957 477 1426 3074
Fragments arranged by size:
3074 1957 1426 552 477
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3718 4730 5513 5530 5683 5868 6313
Size: 1255 2138 1012 783 17 153 185 445
Cuts at: 6313 325
Size: 1498
Fragments arranged by size:
2138 1498 1255 1012 783 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5732 7444 96
Size: 274 80 33 51 1443 3755 1712 138
Fragments arranged by size:
3755 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2554 2586 4021 4053 7446 7478 2554
Size: 32 1435 32 3393 32 2562
Fragments arranged by size:
3393 2562 1435 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7486
XbaI T'CTAG_A
Cuts at: 2233 2392 4822 2233
Size: 159 2430 4897
Fragments arranged by size:
4897 2430 159
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3533 4018 4274 3533
Size: 485 256 6745
Fragments arranged by size:
6745 485 256
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7486
XmaI C'CCGG_G
Cuts at: 1870 4811 1870
Size: 2941 4545
XmnI GAAnn'nnTTC
Cuts at: 2214 2697 3297 5615 2214
Size: 483 600 2318 4085
Fragments arranged by size:
4085 2318 600 483
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5294 276
Size: 53 83 186 936 3760 2468
Fragments arranged by size:
3760 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BsiWI BstXI BstZ17I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI