Restriction map for
pFN21ASDA0891
(Circular) (Six-base) MAPSORT of: pFN21ASDA0891.seq Check: 3874 from: 1 to: 8683
pFN21ASDA0891 8683 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2483 3052 3545 2483
Size: 569 493 7621
Fragments arranged by size:
7621 569 493
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6493 278
Size: 53 83 186 936 4957 2468
Fragments arranged by size:
4957 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5610 6026 1937
Size: 3673 416 4594
Fragments arranged by size:
4594 3673 416
Acc65I G'GTAC_C
Cuts at: 4944 6004 4944
Size: 1060 7623
AclI AA'CG_TT
Cuts at: 1563 6810 7183 1563
Size: 5247 373 3063
Fragments arranged by size:
5247 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4457 6746 8130 8398 1632
Size: 2825 2289 1384 268 1917
Fragments arranged by size:
2825 2289 1917 1384 268
AfeI AGC'GCT
Cuts at: 3158 4442 5105 5348 3158
Size: 1284 663 243 6493
Fragments arranged by size:
6493 1284 663 243
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8486
AflIII A'CryG_T
Cuts at: 1203 2922 5630 5940 7582 8559 1203
Size: 1719 2708 310 1642 977 1327
Fragments arranged by size:
2708 1719 1642 1327 977 310
AgeI A'CCGG_T
Cuts at: 7514 7514
Size: 8683
AhdI GACnn_n'nnGTC
Cuts at: 4273 7412 4273
Size: 3139 5544
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8683
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2591 4712 1184
Size: 1407 2121 5155
Fragments arranged by size:
5155 2121 1407
AlwNI CAG_nnn'CTG
Cuts at: 2828 3565 4820 5165 5773 6426 7998 2828
Size: 737 1255 345 608 653 1572 3513
Fragments arranged by size:
3513 1572 1255 737 653 608 345
ApaI G_GGCC'C
Cuts at: 2064 2903 3042 4881 5814 5832 5849 2064
Size: 839 139 1839 933 18 17 4898
Fragments arranged by size:
4898 1839 933 839 139 18 17
ApaLI G'TGCA_C
Cuts at: 2918 3097 5529 6740 7896 2918
Size: 179 2432 1211 1156 3705
Fragments arranged by size:
3705 2432 1211 1156 179
ApoI r'AATT_y
Cuts at: 1493 2698 4851 5992 6218 6370 8479 1493
Size: 1205 2153 1141 226 152 2109 1697
Fragments arranged by size:
2153 2109 1697 1205 1141 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7237 160
Size: 872 6205 1606
Fragments arranged by size:
6205 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8683
AvaI C'yCGr_G
Cuts at: 1870 1943 2735 2849 3028 3288 3576 4089 4803
Size: 73 792 114 179 260 288 513 714
Cuts at: 4803 5181 5246 6008 1870
Size: 378 65 762 4545
Fragments arranged by size:
4545 792 762 714 513 378 288 260
179 114 73 65
AvrII C'CTAG_G
Cuts at: 2138 5719 2138
Size: 3581 5102
BamHI G'GATC_C
Cuts at: 5155 6013 6380 5155
Size: 858 367 7458
Fragments arranged by size:
7458 858 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2599 3424 3701 4042 4369
Size: 325 217 627 812 825 277 341 327
Cuts at: 4369 4944 5242 5313 5794 5906 6004 7459 618
Size: 575 298 71 481 112 98 1455 1842
Fragments arranged by size:
1842 1455 825 812 627 575 481 341
327 325 298 277 217 112 98 71
BanII G_rGCy'C
Cuts at: 1391 2064 2690 2850 2903 3042 4098 4682 4761
Size: 673 626 160 53 139 1056 584 79
Cuts at: 4761 4881 5814 5832 5849 6002 1391
Size: 120 933 18 17 153 4072
Fragments arranged by size:
4072 1056 933 673 626 584 160 153
139 120 79 53 18 17
BbeI G_GCGC'C
Cuts at: 5317 5317
Size: 8683
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2337 2509 928
Size: 971 438 172 7102
Fragments arranged by size:
7102 971 438 172
BbvCI CC'TCA_GC
Cuts at: 2212 3903 4782 5151 5733 5781 2212
Size: 1691 879 369 582 48 5114
Fragments arranged by size:
5114 1691 879 582 369 48
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2087 2121 5459 5493 6874 6908 1903
Size: 34 150 34 3338 34 1381 34 3678
Fragments arranged by size:
3678 3338 1381 150 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5968 6575 7785 5968
Size: 607 1210 6866
Fragments arranged by size:
6866 1210 607
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8683
BfrBI ATG'CAT
Cuts at: 3420 3420
Size: 8683
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2685 2711 3743
Size: 107 122 71 631 605 1013 26 1032
Cuts at: 3743 5174 5750 7294 136
Size: 1431 576 1544 1525
Fragments arranged by size:
1544 1525 1431 1032 1013 631 605 576
122 107 71 26
BglII A'GATC_T
Cuts at: 2937 8678 2937
Size: 5741 2942
BlpI GC'TnA_GC
Cuts at: 6100 6100
Size: 8683
Bme1580I G_kGCm'C
Cuts at: 1790 2064 2903 2922 3042 3101 3704 3992 4047
Size: 274 839 19 120 59 603 288 55
Cuts at: 4047 4374 4839 4881 5533 5814 5832 5849 5909
Size: 327 465 42 652 281 18 17 60
Cuts at: 5909 6744 7900 1790
Size: 835 1156 2573
Fragments arranged by size:
2573 1156 839 835 652 603 465 327
288 281 274 120 60 59 55 42
19 18 17
BmgBI CAC'GTC
Cuts at: 1318 5569 1318
Size: 4251 4432
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2741 3440 4978
Size: 416 53 83 399 24 1324 699 1538
Cuts at: 4978 5848 6414 7372 442
Size: 870 566 958 1753
Fragments arranged by size:
1753 1538 1324 958 870 699 566 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8683
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4909 4909
Size: 8683
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2093 2346 2462 2739 3191 4736 4745 5075
Size: 854 253 116 277 452 1545 9 330
Cuts at: 5075 5795 5992 7343 1239
Size: 720 197 1351 2579
Fragments arranged by size:
2579 1545 1351 854 720 452 330 277
253 197 116 9
Bpu10I CC'TnA_GC
Cuts at: 2212 3071 3903 4326 4755 4782 5151 5423 5733
Size: 859 832 423 429 27 369 272 310
Cuts at: 5733 5781 2212
Size: 48 5114
Fragments arranged by size:
5114 859 832 429 423 369 310 272
48 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2283 2938 5327 6808 7673 7971 2283
Size: 655 2389 1481 865 298 2995
Fragments arranged by size:
2995 2389 1481 865 655 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2160 3857 4012 4731 7346 882
Size: 617 661 1697 155 719 2615 2219
Fragments arranged by size:
2615 2219 1697 719 661 617 155
BsaAI yAC'GTr
Cuts at: 493 1204 2613 2923 2929 5858 5980 6474 493
Size: 711 1409 310 6 2929 122 494 2702
Fragments arranged by size:
2929 2702 1409 711 494 310 122 6
BsaBI GATnn'nnATC
Cuts at: 2133 8677 2133
Size: 6544 2139
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3655 5314 6490 6872
Size: 53 83 186 936 2122 1659 1176 382
Cuts at: 6872 7507 275
Size: 635 1451
Fragments arranged by size:
2122 1659 1451 1176 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2766 5889 7116 7514 7788 7935 8346 2766
Size: 3123 1227 398 274 147 411 3103
Fragments arranged by size:
3123 3103 1227 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2344 2374 2404 3447 3477
Size: 30 165 30 941 30 30 1043 30
Cuts at: 3477 4743 4773 5105 5135 5243 5273 1178
Size: 1266 30 332 30 108 30 4588
Fragments arranged by size:
4588 1266 1043 941 332 165 108 30
30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2286 2318 2708 2819 3296 3482 3485 4283
Size: 1100 32 390 111 477 186 3 798
Cuts at: 4283 5264 5465 5798 5801 1186
Size: 981 201 333 3 4068
Fragments arranged by size:
4068 1100 981 798 477 390 333 201
186 111 32 3 3
BseYI C'CCAG_C
Cuts at: 2228 3159 4373 4721 4838 5059 5462 7886 8382
Size: 931 1214 348 117 221 403 2424 496
Cuts at: 8382 2228
Size: 2529
Fragments arranged by size:
2529 2424 1214 931 496 403 348 221
117
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2412 2561 3008 3168 3806 4416 4672
Size: 573 676 149 447 160 638 610 256
Cuts at: 4672 5357 1163
Size: 685 4489
Fragments arranged by size:
4489 685 676 638 610 573 447 256
160 149
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6133 6894 7922 1540
Size: 275 186 4132 761 1028 2301
Fragments arranged by size:
4132 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2922 3101 3259 4761 5533 5709 5937 6744 6829
Size: 179 158 1502 772 176 228 807 85
Cuts at: 6829 7900 2922
Size: 1071 3705
Fragments arranged by size:
3705 1502 1071 807 772 228 179 176
158 85
BsmI GAATG_Cn'
Cuts at: 3840 4975 6194 6287 3840
Size: 1135 1219 93 6236
Fragments arranged by size:
6236 1219 1135 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2690 2850 2903 2922 3042 3101
Size: 399 274 626 160 53 19 120 59
Cuts at: 3101 3259 3704 3992 4047 4098 4374 4682 4761
Size: 158 445 288 55 51 276 308 79
Cuts at: 4761 4839 4881 5533 5709 5814 5832 5849 5909
Size: 78 42 652 176 105 18 17 60
Cuts at: 5909 5937 6002 6744 6829 7900 1391
Size: 28 65 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 652 626 445 399 308
288 276 274 176 160 158 120 105
85 79 78 65 60 59 55 53
51 42 28 19 18 17
BspEI T'CCGG_A
Cuts at: 2766 8346 2766
Size: 5580 3103
BspHI T'CATG_A
Cuts at: 2338 3543 6572 8302 2338
Size: 1205 3029 1730 2719
Fragments arranged by size:
3029 2719 1730 1205
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2483 2684 3052 3545 4212 4570 5684 6038
Size: 1639 201 368 493 667 358 1114 354
Cuts at: 6038 844
Size: 3489
Fragments arranged by size:
3489 1639 1114 667 493 368 358 354
201
BsrBI CCG'CTC
Cuts at: 1394 1823 3606 4333 6570 1394
Size: 429 1783 727 2237 3507
Fragments arranged by size:
3507 2237 1783 727 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 7178 7352 65
Size: 1183 5930 174 1396
Fragments arranged by size:
5930 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4039 5168 5186 5658 5791 7327 7514
Size: 110 2229 1129 18 472 133 1536 187
Cuts at: 7514 1700
Size: 2869
Fragments arranged by size:
2869 2229 1536 1129 472 187 133 110
18
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8683
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8683
BssSI C'ACGA_G
Cuts at: 3186 3824 5930 6743 7755 3186
Size: 638 2106 813 1012 4114
Fragments arranged by size:
4114 2106 1012 813 638
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2825 2828 3206 3537 5305 5433 794
Size: 2031 3 378 331 1768 128 4044
Fragments arranged by size:
4044 2031 1768 378 331 128 3
BstBI TT'CG_AA
Cuts at: 2007 7487 2007
Size: 5480 3203
BstEII G'GTnAC_C
Cuts at: 1184 4768 1184
Size: 3584 5099
BstXI CCAn_nnnn'nTGG
Cuts at: 2228 5140 2228
Size: 2912 5771
BstYI r'GATC_y
Cuts at: 1972 2134 2937 5155 6013 6380 6765 6782 8234
Size: 162 803 2218 858 367 385 17 1452
Cuts at: 8234 8320 8332 8678 1972
Size: 86 12 346 1977
Fragments arranged by size:
2218 1977 1452 858 803 385 367 346
162 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2194 3017 5232 5799 2194
Size: 823 2215 567 5078
Fragments arranged by size:
5078 2215 823 567
BtgI C'CryG_G
Cuts at: 513 1065 2760 3363 3867 5910 513
Size: 552 1695 603 504 2043 3286
Fragments arranged by size:
3286 2043 1695 603 552 504
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7944
Fragments arranged by size:
7944 683 56
BtsI GCAGTG_nn'
Cuts at: 2510 2585 2733 2820 3101 4146 4694 5520 5569
Size: 75 148 87 281 1045 548 826 49
Cuts at: 5569 6199 6993 7013 2510
Size: 630 794 20 4180
Fragments arranged by size:
4180 1045 826 794 630 548 281 148
87 75 49 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8683
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2820 2855 529
Size: 35 2256 35 6357
Fragments arranged by size:
6357 2256 35 35
DraI TTT'AAA
Cuts at: 5987 5987
Size: 8683
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7690 809
Size: 507 6374 1802
Fragments arranged by size:
6374 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3660 4869 5188
Size: 54 1425 50 275 18 1830 1209 319
Cuts at: 5188 5512 6130 7019 8648 8
Size: 324 618 889 1629 43
Fragments arranged by size:
1830 1629 1425 1209 889 618 324 319
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6130 1537
Size: 275 4318 4090
Fragments arranged by size:
4318 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2391 3471 3477 5225 5654 6613 1353
Size: 1038 1080 6 1748 429 959 3423
Fragments arranged by size:
3423 1748 1080 1038 959 429 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4229 5107 5677 7270 7644 7790
Size: 1064 138 2640 878 570 1593 374 146
Cuts at: 7790 387
Size: 1280
Fragments arranged by size:
2640 1593 1280 1064 878 570 374 146
138
EcoICRI GAG'CTC
Cuts at: 4759 4759
Size: 8683
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2346 2462 2739 3191 4457 4736
Size: 393 461 253 116 277 452 1266 279
Cuts at: 4736 4745 5075 5795 5992 6746 7343 8130 8398
Size: 9 330 720 197 754 597 787 268
Cuts at: 8398 1239
Size: 1524
Fragments arranged by size:
1524 1266 787 754 720 597 461 452
393 330 279 277 268 253 197 116
9
EcoNI CCTnn'n_nnAGG
Cuts at: 2630 2712 3959 2630
Size: 82 1247 7354
Fragments arranged by size:
7354 1247 82
EcoO109I rG'GnC_Cy
Cuts at: 2060 2061 2429 2899 3039 3166 3175 3829 4513
Size: 1 368 470 140 127 9 654 684
Cuts at: 4513 4829 5682 5723 5811 5819 5828 5829 5846
Size: 316 853 41 88 8 9 1 17
Cuts at: 5846 2060
Size: 4897
Fragments arranged by size:
4897 853 684 654 470 368 316 140
127 88 41 17 9 9 8 1
1
EcoRI G'AATT_C
Cuts at: 5992 6370 5992
Size: 378 8305
FseI GG_CCGG'CC
Cuts at: 5190 5190
Size: 8683
FspI TGC'GCA
Cuts at: 7189 7189
Size: 8683
HaeII r_GCGC'y
Cuts at: 1678 3160 3692 4363 4444 5107 5317 5350 5581
Size: 1482 532 671 81 663 210 33 231
Cuts at: 5581 7830 1678
Size: 2249 2531
Fragments arranged by size:
2531 2249 1482 671 663 532 231 210
81 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2138 2140 2170 2172 2735
Size: 32 135 32 566 2 30 2 563
Cuts at: 2735 2767 2837 2869 3136 3168 4157 4189 4259
Size: 32 70 32 267 32 989 32 70
Cuts at: 4259 4291 4295 4327 4901 4933 5147 5179 7324
Size: 32 4 32 574 32 214 32 2145
Cuts at: 7324 7356 7398 7430 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2145 989 574 566 563 267 214
135 70 70 42 32 32 32 32
32 32 32 32 32 32 32 32
30 4 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 6027 6273 669
Size: 988 281 4089 246 3079
Fragments arranged by size:
4089 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2411 748
Size: 1663 7020
HpaI GTT'AAC
Cuts at: 6273 6273
Size: 8683
KasI G'GCGC_C
Cuts at: 5313 5313
Size: 8683
KpnI G_GTAC'C
Cuts at: 4948 6008 4948
Size: 1060 7623
MfeI C'AATT_G
Cuts at: 6282 6282
Size: 8683
MluI A'CGCG_T
Cuts at: 5630 8559 5630
Size: 2929 5754
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3248 3594 4401 5037 7797 7981 1305
Size: 1943 346 807 636 2760 184 2007
Fragments arranged by size:
2760 2007 1943 807 636 346 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3662 4871 5514 10
Size: 54 1425 343 1830 1209 643 3179
Fragments arranged by size:
3179 1830 1425 1209 643 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3362 4956 6641 7000 7159 518
Size: 648 2196 1594 1685 359 159 2042
Fragments arranged by size:
2196 2042 1685 1594 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2228 2440 2662 3919 4408 4765 4904 5026
Size: 622 212 222 1257 489 357 139 122
Cuts at: 5026 5302 5458 5732 6099 6776 7924 8169 1606
Size: 276 156 274 367 677 1148 245 2120
Fragments arranged by size:
2120 1257 1148 677 622 489 367 357
276 274 245 222 212 156 139 122
NaeI GCC'GGC
Cuts at: 5188 5660 5188
Size: 472 8211
NarI GG'CG_CC
Cuts at: 5314 5314
Size: 8683
NcoI C'CATG_G
Cuts at: 513 1065 2760 3363 513
Size: 552 1695 603 5833
Fragments arranged by size:
5833 1695 603 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8683
NgoMIV G'CCGG_C
Cuts at: 5186 5658 5186
Size: 472 8211
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8683
NotI GC'GGCC_GC
Cuts at: 6130 6130
Size: 8683
NsiI A_TGCA'T
Cuts at: 3422 3422
Size: 8683
NspI r_CATG'y
Cuts at: 1132 1229 3374 3420 6041 7586 1132
Size: 97 2145 46 2621 1545 2229
Fragments arranged by size:
2621 2229 2145 1545 97 46
PasI CC'CwG_GG
Cuts at: 1348 2780 5688 5815 5823 1348
Size: 1432 2908 127 8 4208
Fragments arranged by size:
4208 2908 1432 127 8
PciI A'CATG_T
Cuts at: 7582 7582
Size: 8683
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3452 4264 1108
Size: 2344 812 5527
Fragments arranged by size:
5527 2344 812
PfoI T'CCnGG_A
Cuts at: 3585 5756 3585
Size: 2171 6512
PmeI GTTT'AAAC
Cuts at: 5987 5987
Size: 8683
PmlI CAC'GTG
Cuts at: 2613 2923 5858 2613
Size: 310 2935 5438
Fragments arranged by size:
5438 2935 310
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6752 6784 8265 8297 6752
Size: 32 1481 32 7138
Fragments arranged by size:
7138 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2429 3829 4829 5819 2429
Size: 1400 1000 990 5293
Fragments arranged by size:
5293 1400 1000 990
PshAI GACnn'nnGTC
Cuts at: 1613 4827 1613
Size: 3214 5469
PsiI TTA'TAA
Cuts at: 6253 6253
Size: 8683
PspOMI G'GGCC_C
Cuts at: 2060 2899 3038 4877 5810 5828 5845 2060
Size: 839 139 1839 933 18 17 4898
Fragments arranged by size:
4898 1839 933 839 139 18 17
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3839 3871 4523 4555 3839
Size: 32 652 32 7967
Fragments arranged by size:
7967 652 32 32
PstI C_TGCA'G
Cuts at: 830 2439 2945 5731 6035 830
Size: 1609 506 2786 304 3478
Fragments arranged by size:
3478 2786 1609 506 304
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8683
PvuII CAG'CTG
Cuts at: 2228 2440 4765 4904 5302 5458 5732 2228
Size: 212 2325 139 398 156 274 5179
Fragments arranged by size:
5179 2325 398 274 212 156 139
SacI G_AGCT'C
Cuts at: 4761 4761
Size: 8683
SalI G'TCGA_C
Cuts at: 1936 6025 1936
Size: 4089 4594
SanDI GG'GwC_CC
Cuts at: 5819 5819
Size: 8683
SbfI CC_TGCA'GG
Cuts at: 6035 6035
Size: 8683
ScaI AGT'ACT
Cuts at: 2961 6931 8643 2961
Size: 3970 1712 3001
Fragments arranged by size:
3970 3001 1712
SfcI C'TryA_G
Cuts at: 826 1045 2435 2941 5071 5727 6031 7166 7847
Size: 219 1390 506 2130 656 304 1135 681
Cuts at: 7847 8038 826
Size: 191 1471
Fragments arranged by size:
2130 1471 1390 1135 681 656 506 304
219 191
SfoI GGC'GCC
Cuts at: 5315 5315
Size: 8683
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8683
SgrAI Cr'CCGG_yG
Cuts at: 1700 5658 1700
Size: 3958 4725
SmaI CCC'GGG
Cuts at: 1872 2737 3030 3290 4805 5183 6010 1872
Size: 865 293 260 1515 378 827 4545
Fragments arranged by size:
4545 1515 865 827 378 293 260
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2298 2953 5342 6787 7688 7950
Size: 197 926 355 655 2389 1445 901 262
Cuts at: 7950 820
Size: 1553
Fragments arranged by size:
2389 1553 1445 926 901 655 355 262
197
SnaBI TAC'GTA
Cuts at: 493 5980 6474 493
Size: 5487 494 2702
Fragments arranged by size:
5487 2702 494
SpeI A'CTAG_T
Cuts at: 152 2084 152
Size: 1932 6751
SphI G_CATG'C
Cuts at: 1132 6041 1132
Size: 4909 3774
SrfI GCCC'GGGC
Cuts at: 3290 5183 3290
Size: 1893 6790
SspI AAT'ATT
Cuts at: 5 52 6607 5
Size: 47 6555 2081
Fragments arranged by size:
6555 2081 47
StuI AGG'CCT
Cuts at: 1517 5255 5291 1517
Size: 3738 36 4909
Fragments arranged by size:
4909 3738 36
StyI C'CwwG_G
Cuts at: 513 1065 2051 2138 2760 2799 2835 3312 3363
Size: 552 986 87 622 39 36 477 51
Cuts at: 3363 4002 4665 5133 5719 6122 513
Size: 639 663 468 586 403 3074
Fragments arranged by size:
3074 986 663 639 622 586 552 477
468 403 87 51 39 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3182 3376 3719 5051 5727 5830 6710
Size: 1255 1602 194 343 1332 676 103 880
Cuts at: 6710 6727 6880 7065 7510 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1602 1498 1332 1255 880 676 445 343
194 185 153 103 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2959 3969 5644
Size: 274 80 33 51 1443 982 1010 1675
Cuts at: 5644 6929 8641 96
Size: 1285 1712 138
Fragments arranged by size:
1712 1675 1443 1285 1010 982 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2374 2406 3447 3479 4743 4775 4783 4815 8643
Size: 32 1041 32 1264 32 8 32 3828
Cuts at: 8643 8675 2374
Size: 32 2382
Fragments arranged by size:
3828 2382 1264 1041 32 32 32 32
32 8
Tth111I GACn'n_nGTC
Cuts at: 1316 2152 2747 1316
Size: 836 595 7252
Fragments arranged by size:
7252 836 595
XbaI T'CTAG_A
Cuts at: 6019 6019
Size: 8683
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2806 3002 4117 4711 2806
Size: 196 1115 594 6778
Fragments arranged by size:
6778 1115 594 196
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8683
XmaI C'CCGG_G
Cuts at: 1870 2735 3028 3288 4803 5181 6008 1870
Size: 865 293 260 1515 378 827 4545
Fragments arranged by size:
4545 1515 865 827 378 293 260
XmnI GAAnn'nnTTC
Cuts at: 4855 6812 4855
Size: 1957 6726
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6491 276
Size: 53 83 186 936 4957 2468
Fragments arranged by size:
4957 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI BaeI BsiWI BsmBI BstZ17I DraIII EcoRV
FalI FspAI NruI PacI PspXI RsrII SacII SapI
SexAI SfiI SwaI