Restriction map for
pFN21ASDA0888
(Circular) (Six-base) MAPSORT of: pFN21ASDA0888.seq Check: 5541 from: 1 to: 6739
pFN21ASDA0888 6739 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2329 2329
Size: 6739
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4549 278
Size: 53 83 186 936 3013 2468
Fragments arranged by size:
3013 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2687 3134 3496 3963 4082 1937
Size: 750 447 362 467 119 4594
Fragments arranged by size:
4594 750 467 447 362 119
Acc65I G'GTAC_C
Cuts at: 2766 4060 2766
Size: 1294 5445
AclI AA'CG_TT
Cuts at: 1563 4866 5239 1563
Size: 3303 373 3063
Fragments arranged by size:
3303 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2103 2259 2922 3888 4802 6186 6454 1632
Size: 471 156 663 966 914 1384 268 1917
Fragments arranged by size:
1917 1384 966 914 663 471 268 156
AflII C'TTAA_G
Cuts at: 820 1017 2333 3329 820
Size: 197 1316 996 4230
Fragments arranged by size:
4230 1316 996 197
AflIII A'CryG_T
Cuts at: 1203 2091 5638 6615 1203
Size: 888 3547 977 1327
Fragments arranged by size:
3547 1327 977 888
AgeI A'CCGG_T
Cuts at: 3685 5570 3685
Size: 1885 4854
AhdI GACnn_n'nnGTC
Cuts at: 2911 5468 2911
Size: 2557 4182
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6739
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6739
AlwNI CAG_nnn'CTG
Cuts at: 4482 6054 4482
Size: 1572 5167
ApaLI G'TGCA_C
Cuts at: 4796 5952 4796
Size: 1156 5583
ApoI r'AATT_y
Cuts at: 1493 2074 2217 2814 3059 3164 3200 3671 3697
Size: 581 143 597 245 105 36 471 26
Cuts at: 3697 3973 4048 4274 4426 6535 1493
Size: 276 75 226 152 2109 1697
Fragments arranged by size:
2109 1697 597 581 471 276 245 226
152 143 105 75 36 26
AseI AT'TA_AT
Cuts at: 160 1032 5293 160
Size: 872 4261 1606
Fragments arranged by size:
4261 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6739
AvaI C'yCGr_G
Cuts at: 1870 1943 4064 1870
Size: 73 2121 4545
Fragments arranged by size:
4545 2121 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2750 2783 2750
Size: 33 6706
BamHI G'GATC_C
Cuts at: 4069 4436 4069
Size: 367 6372
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2766 4060 5515 618
Size: 325 217 627 979 1294 1455 1842
Fragments arranged by size:
1842 1455 1294 979 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3158 3759 4058 1391
Size: 1767 601 299 4072
Fragments arranged by size:
4072 1767 601 299
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2667 2910 3363 3423 3735 3819 928
Size: 971 768 243 453 60 312 84 3848
Fragments arranged by size:
3848 971 768 453 312 243 84 60
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4930 4964 1903
Size: 34 2993 34 3678
Fragments arranged by size:
3678 2993 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2676 2727 4631 5841 2676
Size: 51 1904 1210 3574
Fragments arranged by size:
3574 1904 1210 51
BclI T'GATC_A
Cuts at: 1548 2275 1548
Size: 727 6012
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5350 136
Size: 107 122 71 631 605 3678 1525
Fragments arranged by size:
3678 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6734 6734
Size: 6739
BlpI GC'TnA_GC
Cuts at: 4023 4156 4023
Size: 133 6606
Bme1580I G_kGCm'C
Cuts at: 1790 3549 4800 5956 1790
Size: 1759 1251 1156 2573
Fragments arranged by size:
2573 1759 1251 1156
BmgBI CAC'GTC
Cuts at: 1318 3817 1318
Size: 2499 4240
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4470 5428 442
Size: 416 53 83 399 24 3053 958 1753
Fragments arranged by size:
3053 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6739
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3376 3376
Size: 6739
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2639 2877 5399 1239
Size: 1400 238 2522 2579
Fragments arranged by size:
2579 2522 1400 238
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3057 4864 5729 6027 3057
Size: 1807 865 298 3769
Fragments arranged by size:
3769 1807 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2718 3694 5402 882
Size: 617 1219 976 1708 2219
Fragments arranged by size:
2219 1708 1219 976 617
BsaAI yAC'GTr
Cuts at: 493 1204 3494 4036 4530 493
Size: 711 2290 542 494 2702
Fragments arranged by size:
2702 2290 711 542 494
BsaBI GATnn'nnATC
Cuts at: 6733 6733
Size: 6739
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4546 4928 5563 275
Size: 53 83 186 936 3013 382 635 1451
Fragments arranged by size:
3013 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3685 5172 5570 5844 5991 6402 3685
Size: 1487 398 274 147 411 4022
Fragments arranged by size:
4022 1487 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6514
Fragments arranged by size:
6514 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3755 3872 1186
Size: 2569 117 4053
Fragments arranged by size:
4053 2569 117
BseYI C'CCAG_C
Cuts at: 3249 5942 6438 3249
Size: 2693 496 3550
Fragments arranged by size:
3550 2693 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3123 1163
Size: 573 1387 4779
Fragments arranged by size:
4779 1387 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4189 4950 5978 1540
Size: 275 186 2188 761 1028 2301
Fragments arranged by size:
2301 2188 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2348 3158 4800 4885 5956 2348
Size: 810 1642 85 1071 3131
Fragments arranged by size:
3131 1642 1071 810 85
BsmI GAATG_Cn'
Cuts at: 2027 3838 4250 4343 2027
Size: 1811 412 93 4423
Fragments arranged by size:
4423 1811 412 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2348 3158 3549 3759 4058 4800 4885
Size: 399 558 810 391 210 299 742 85
Cuts at: 4885 5956 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 810 742 558 399 391 299
210 85
BspEI T'CCGG_A
Cuts at: 6402 6402
Size: 6739
BspHI T'CATG_A
Cuts at: 2977 4628 6358 2977
Size: 1651 1730 3358
Fragments arranged by size:
3358 1730 1651
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2329 4015 4094 844
Size: 1485 1686 79 3489
Fragments arranged by size:
3489 1686 1485 79
BsrBI CCG'CTC
Cuts at: 1394 1823 4626 1394
Size: 429 2803 3507
Fragments arranged by size:
3507 2803 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5234 5408 65
Size: 1183 3986 174 1396
Fragments arranged by size:
3986 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3685 5383 5570 1700
Size: 110 1875 1698 187 2869
Fragments arranged by size:
2869 1875 1698 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6739
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6739
BssSI C'ACGA_G
Cuts at: 4799 5811 4799
Size: 1012 5727
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6739
BstBI TT'CG_AA
Cuts at: 2007 5543 2007
Size: 3536 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6739
BstYI r'GATC_y
Cuts at: 1972 2421 4069 4436 4821 4838 6290 6376 6388
Size: 449 1648 367 385 17 1452 86 12
Cuts at: 6388 6734 1972
Size: 346 1977
Fragments arranged by size:
1977 1648 1452 449 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2688 3497 2688
Size: 809 5930
Bsu36I CC'TnA_GG
Cuts at: 3465 3465
Size: 6739
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5230
Fragments arranged by size:
5230 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6000
Fragments arranged by size:
6000 683 56
BtsI GCAGTG_nn'
Cuts at: 3223 4255 5049 5069 3223
Size: 1032 794 20 4893
Fragments arranged by size:
4893 1032 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6739
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6704
DraI TTT'AAA
Cuts at: 4043 4043
Size: 6739
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5746 809
Size: 507 4430 1802
Fragments arranged by size:
4430 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3026 3233 4186
Size: 54 1425 50 275 18 1196 207 953
Cuts at: 4186 5075 6704 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1196 953 889 275 207 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4186 1537
Size: 275 2374 4090
Fragments arranged by size:
4090 2374 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2145 3345 3549 3783 4669 1353
Size: 792 1200 204 234 886 3423
Fragments arranged by size:
3423 1200 886 792 234 204
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3985 5326 5700 5846 387
Size: 1064 138 2396 1341 374 146 1280
Fragments arranged by size:
2396 1341 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3156 3156
Size: 6739
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2103 2259 2639 2877 2922 3888 4802
Size: 393 471 156 380 238 45 966 914
Cuts at: 4802 5399 6186 6454 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 966 914 787 597 471 393 380
268 238 156 45
EcoO109I rG'GnC_Cy
Cuts at: 2113 4004 2113
Size: 1891 4848
EcoRI G'AATT_C
Cuts at: 3164 4048 4426 3164
Size: 884 378 5477
Fragments arranged by size:
5477 884 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2669 2722 3608 2669
Size: 53 886 5800
Fragments arranged by size:
5800 886 53
FspI TGC'GCA
Cuts at: 5245 5245
Size: 6739
HaeII r_GCGC'y
Cuts at: 1678 5886 1678
Size: 4208 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2078 2110 2897 2929 2972
Size: 32 135 32 506 32 787 32 43
Cuts at: 2972 3004 3548 3580 3686 3718 3734 3766 3848
Size: 32 544 32 106 32 16 32 82
Cuts at: 3848 3880 3914 3946 5380 5412 5454 5486 1373
Size: 32 34 32 1434 32 42 32 2626
Fragments arranged by size:
2626 1434 787 544 506 135 106 82
43 42 34 32 32 32 32 32
32 32 32 32 32 32 32 16
HincII GTy'rAC
Cuts at: 669 1657 1938 4083 4329 669
Size: 988 281 2145 246 3079
Fragments arranged by size:
3079 2145 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6739
HpaI GTT'AAC
Cuts at: 4329 4329
Size: 6739
KpnI G_GTAC'C
Cuts at: 2770 4064 2770
Size: 1294 5445
MfeI C'AATT_G
Cuts at: 2549 4338 2549
Size: 1789 4950
MluI A'CGCG_T
Cuts at: 6615 6615
Size: 6739
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2737 5853 6037 1305
Size: 1432 3116 184 2007
Fragments arranged by size:
3116 2007 1432 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4917
Fragments arranged by size:
4917 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2431 4697 5056 5215 518
Size: 648 1265 2266 359 159 2042
Fragments arranged by size:
2266 2042 1265 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2255 4155 4832 5980 6225 1606
Size: 649 1900 677 1148 245 2120
Fragments arranged by size:
2120 1900 1148 677 649 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5230
Fragments arranged by size:
5230 957 552
NdeI CA'TA_TG
Cuts at: 387 2519 387
Size: 2132 4607
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6739
NotI GC'GGCC_GC
Cuts at: 4186 4186
Size: 6739
NspI r_CATG'y
Cuts at: 1132 1229 2095 2617 4097 5642 1132
Size: 97 866 522 1480 1545 2229
Fragments arranged by size:
2229 1545 1480 866 522 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6739
PciI A'CATG_T
Cuts at: 2091 5638 2091
Size: 3547 3192
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3035 1108
Size: 1927 4812
PfoI T'CCnGG_A
Cuts at: 2719 3168 2719
Size: 449 6290
PmeI GTTT'AAAC
Cuts at: 4043 4043
Size: 6739
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4808 4840 6321 6353 4808
Size: 32 1481 32 5194
Fragments arranged by size:
5194 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2113 4004 2113
Size: 1891 4848
PshAI GACnn'nnGTC
Cuts at: 1613 2851 1613
Size: 1238 5501
PsiI TTA'TAA
Cuts at: 3344 4309 3344
Size: 965 5774
PstI C_TGCA'G
Cuts at: 830 4091 830
Size: 3261 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6739
PvuII CAG'CTG
Cuts at: 2255 2255
Size: 6739
SacI G_AGCT'C
Cuts at: 3158 3158
Size: 6739
SalI G'TCGA_C
Cuts at: 1936 4081 1936
Size: 2145 4594
SanDI GG'GwC_CC
Cuts at: 2113 2113
Size: 6739
SapI GCTCTTCn'nnn_
Cuts at: 3783 3783
Size: 6739
SbfI CC_TGCA'GG
Cuts at: 4091 4091
Size: 6739
ScaI AGT'ACT
Cuts at: 2446 4987 6699 2446
Size: 2541 1712 2486
Fragments arranged by size:
2541 2486 1712
SfcI C'TryA_G
Cuts at: 826 1045 2508 4087 5222 5903 6094 826
Size: 219 1463 1579 1135 681 191 1471
Fragments arranged by size:
1579 1471 1463 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6739
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6739
SmaI CCC'GGG
Cuts at: 1872 4066 1872
Size: 2194 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2333 3072 3329 4843 5744 6006
Size: 197 926 390 739 257 1514 901 262
Cuts at: 6006 820
Size: 1553
Fragments arranged by size:
1553 1514 926 901 739 390 262 257
197
SnaBI TAC'GTA
Cuts at: 493 4036 4530 493
Size: 3543 494 2702
Fragments arranged by size:
3543 2702 494
SpeI A'CTAG_T
Cuts at: 152 3365 152
Size: 3213 3526
SphI G_CATG'C
Cuts at: 1132 4097 1132
Size: 2965 3774
SspI AAT'ATT
Cuts at: 5 52 2149 4663 5
Size: 47 2097 2514 2081
Fragments arranged by size:
2514 2097 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6739
StyI C'CwwG_G
Cuts at: 513 1065 2022 4178 513
Size: 552 957 2156 3074
Fragments arranged by size:
3074 2156 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4766 4783 4936 5121 5566 325
Size: 1255 3186 17 153 185 445 1498
Fragments arranged by size:
3186 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2444 4985 6697
Size: 274 80 33 51 1443 467 2541 1712
Cuts at: 6697 96
Size: 138
Fragments arranged by size:
2541 1712 1443 467 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6699 6731 6699
Size: 32 6707
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6739
XbaI T'CTAG_A
Cuts at: 4075 4075
Size: 6739
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6739
XmaI C'CCGG_G
Cuts at: 1870 4064 1870
Size: 2194 4545
XmnI GAAnn'nnTTC
Cuts at: 4868 4868
Size: 6739
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4547 276
Size: 53 83 186 936 3013 2468
Fragments arranged by size:
3013 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI DraIII EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI
SfoI SrfI SwaI XcmI