Restriction map for
pFN21ASDA0887
(Circular) (Six-base) MAPSORT of: pFN21ASDA0887.seq Check: 5910 from: 1 to: 6064
pFN21ASDA0887 6064 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3874 278
Size: 53 83 186 936 2338 2468
Fragments arranged by size:
2468 2338 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3407 1937
Size: 1470 4594
Acc65I G'GTAC_C
Cuts at: 3385 3385
Size: 6064
AclI AA'CG_TT
Cuts at: 1563 4191 4564 1563
Size: 2628 373 3063
Fragments arranged by size:
3063 2628 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2631 2756 3255 4127 5511 5779 1632
Size: 999 125 499 872 1384 268 1917
Fragments arranged by size:
1917 1384 999 872 499 268 125
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5867
AflIII A'CryG_T
Cuts at: 1203 4963 5940 1203
Size: 3760 977 1327
Fragments arranged by size:
3760 1327 977
AgeI A'CCGG_T
Cuts at: 4895 4895
Size: 6064
AhdI GACnn_n'nnGTC
Cuts at: 4793 4793
Size: 6064
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6064
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6064
AlwNI CAG_nnn'CTG
Cuts at: 3807 5379 3807
Size: 1572 4492
ApaLI G'TGCA_C
Cuts at: 2604 4121 5277 2604
Size: 1517 1156 3391
Fragments arranged by size:
3391 1517 1156
ApoI r'AATT_y
Cuts at: 1493 3034 3373 3599 3751 5860 1493
Size: 1541 339 226 152 2109 1697
Fragments arranged by size:
2109 1697 1541 339 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4618 160
Size: 872 3586 1606
Fragments arranged by size:
3586 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6064
AvaI C'yCGr_G
Cuts at: 1870 1943 3138 3389 1870
Size: 73 1195 251 4545
Fragments arranged by size:
4545 1195 251 73
BamHI G'GATC_C
Cuts at: 3394 3761 3394
Size: 367 5697
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2029 3385 4840 618
Size: 325 217 627 242 1356 1455 1842
Fragments arranged by size:
1842 1455 1356 627 325 242 217
BanII G_rGCy'C
Cuts at: 1391 3383 1391
Size: 1992 4072
BbeI G_GCGC'C
Cuts at: 2033 2033
Size: 6064
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2473 928
Size: 971 574 4519
Fragments arranged by size:
4519 971 574
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4255 4289 1903
Size: 34 2318 34 3678
Fragments arranged by size:
3678 2318 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2669 3956 5166 2669
Size: 1287 1210 3567
Fragments arranged by size:
3567 1287 1210
BclI T'GATC_A
Cuts at: 1548 2563 1548
Size: 1015 5049
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2915 4675 136
Size: 107 122 71 631 605 1243 1760 1525
Fragments arranged by size:
1760 1525 1243 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6059 6059
Size: 6064
BlpI GC'TnA_GC
Cuts at: 3481 3481
Size: 6064
Bme1580I G_kGCm'C
Cuts at: 1790 2473 2608 4125 5281 1790
Size: 683 135 1517 1156 2573
Fragments arranged by size:
2573 1517 1156 683 135
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6064
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3795 4753 442
Size: 416 53 83 399 24 2378 958 1753
Fragments arranged by size:
2378 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6064
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4724 1239
Size: 3485 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2257 4189 5054 5352 2257
Size: 1932 865 298 2969
Fragments arranged by size:
2969 1932 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2689 4727 882
Size: 617 1190 2038 2219
Fragments arranged by size:
2219 2038 1190 617
BsaAI yAC'GTr
Cuts at: 493 1204 3361 3855 493
Size: 711 2157 494 2702
Fragments arranged by size:
2702 2157 711 494
BsaBI GATnn'nnATC
Cuts at: 6058 6058
Size: 6064
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2030 3871 4253 4888
Size: 53 83 186 936 497 1841 382 635
Cuts at: 4888 275
Size: 1451
Fragments arranged by size:
1841 1451 936 635 497 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2331 4497 4895 5169 5316 5727 2331
Size: 2166 398 274 147 411 2668
Fragments arranged by size:
2668 2166 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5839
Fragments arranged by size:
5839 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 2975 3002 3005 3032 3290 1186
Size: 865 924 27 3 27 258 3960
Fragments arranged by size:
3960 924 865 258 27 27 3
BseYI C'CCAG_C
Cuts at: 5267 5763 5267
Size: 496 5568
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5491
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3514 4275 5303 1540
Size: 275 186 1513 761 1028 2301
Fragments arranged by size:
2301 1513 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2608 4125 4210 5281 2608
Size: 1517 85 1071 3391
Fragments arranged by size:
3391 1517 1071 85
BsmI GAATG_Cn'
Cuts at: 3071 3575 3668 3071
Size: 504 93 5467
Fragments arranged by size:
5467 504 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3016 3142 3016
Size: 126 5938
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2473 2608 3383 4125 4210 5281 1391
Size: 399 683 135 775 742 85 1071 2174
Fragments arranged by size:
2174 1071 775 742 683 399 135 85
BspEI T'CCGG_A
Cuts at: 5727 5727
Size: 6064
BspHI T'CATG_A
Cuts at: 3953 5683 3953
Size: 1730 4334
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2221 3419 844
Size: 1377 1198 3489
Fragments arranged by size:
3489 1377 1198
BsrBI CCG'CTC
Cuts at: 1394 1823 2041 2965 3951 1394
Size: 429 218 924 986 3507
Fragments arranged by size:
3507 986 924 429 218
BsrDI GCAATG_nn'
Cuts at: 65 1248 4559 4733 65
Size: 1183 3311 174 1396
Fragments arranged by size:
3311 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4708 4895 1700
Size: 110 2898 187 2869
Fragments arranged by size:
2898 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2161 96
Size: 2065 3999
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6064
BssSI C'ACGA_G
Cuts at: 4124 5136 4124
Size: 1012 5052
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6064
BstBI TT'CG_AA
Cuts at: 2007 4868 2007
Size: 2861 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6064
BstYI r'GATC_y
Cuts at: 1972 2044 2086 2253 3394 3761 4146 4163 5615
Size: 72 42 167 1141 367 385 17 1452
Cuts at: 5615 5701 5713 6059 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1141 385 367 346 167 86
72 42 17 12
Bsu36I CC'TnA_GG
Cuts at: 2034 2670 2034
Size: 636 5428
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4555
Fragments arranged by size:
4555 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5325
Fragments arranged by size:
5325 683 56
BtsI GCAGTG_nn'
Cuts at: 3580 4374 4394 3580
Size: 794 20 5250
Fragments arranged by size:
5250 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6064
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6029
DraI TTT'AAA
Cuts at: 3368 3368
Size: 6064
DraIII CAC_nnn'GTG
Cuts at: 2603 2603
Size: 6064
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5071 809
Size: 507 3755 1802
Fragments arranged by size:
3755 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2721 3511 4400
Size: 54 1425 50 275 18 891 790 889
Cuts at: 4400 6029 8
Size: 1629 43
Fragments arranged by size:
1629 1425 891 889 790 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3511 1537
Size: 275 1699 4090
Fragments arranged by size:
4090 1699 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2379 2448 2648 3994 1353
Size: 1026 69 200 1346 3423
Fragments arranged by size:
3423 1346 1026 200 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2995 4651 5025 5171 387
Size: 1064 138 1406 1656 374 146 1280
Fragments arranged by size:
1656 1406 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2631 2756 3255 4127 4724 5511 5779
Size: 393 999 125 499 872 597 787 268
Cuts at: 5779 1239
Size: 1524
Fragments arranged by size:
1524 999 872 787 597 499 393 268
125
EcoO109I rG'GnC_Cy
Cuts at: 3343 3343
Size: 6064
EcoRI G'AATT_C
Cuts at: 3373 3751 3373
Size: 378 5686
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3133 3133
Size: 6064
FspI TGC'GCA
Cuts at: 4570 4570
Size: 6064
HaeII r_GCGC'y
Cuts at: 1678 2033 5211 1678
Size: 355 3178 2531
Fragments arranged by size:
3178 2531 355
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2667 2699 4705 4737 4779
Size: 32 135 32 1095 32 2006 32 42
Cuts at: 4779 4811 1373
Size: 32 2626
Fragments arranged by size:
2626 2006 1095 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3408 3654 669
Size: 988 281 1470 246 3079
Fragments arranged by size:
3079 1470 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6064
HpaI GTT'AAC
Cuts at: 3654 3654
Size: 6064
KasI G'GCGC_C
Cuts at: 2029 2029
Size: 6064
KpnI G_GTAC'C
Cuts at: 3389 3389
Size: 6064
MfeI C'AATT_G
Cuts at: 3663 3663
Size: 6064
MluI A'CGCG_T
Cuts at: 5940 5940
Size: 6064
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3042 5178 5362 1305
Size: 1737 2136 184 2007
Fragments arranged by size:
2136 2007 1737 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2723 10
Size: 54 1425 343 891 3351
Fragments arranged by size:
3351 1425 891 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2559 2636 2729 4022 4381 4540 518
Size: 648 1393 77 93 1293 359 159 2042
Fragments arranged by size:
2042 1393 1293 648 359 159 93 77
MspA1I CmG'CkG
Cuts at: 1606 2243 3480 4157 5305 5550 1606
Size: 637 1237 677 1148 245 2120
Fragments arranged by size:
2120 1237 1148 677 637 245
NarI GG'CG_CC
Cuts at: 2030 2030
Size: 6064
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4555
Fragments arranged by size:
4555 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6064
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6064
NotI GC'GGCC_GC
Cuts at: 3511 3511
Size: 6064
NspI r_CATG'y
Cuts at: 1132 1229 2655 3422 4967 1132
Size: 97 1426 767 1545 2229
Fragments arranged by size:
2229 1545 1426 767 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6064
PciI A'CATG_T
Cuts at: 4963 4963
Size: 6064
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2128 1108
Size: 1020 5044
PmeI GTTT'AAAC
Cuts at: 3368 3368
Size: 6064
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4133 4165 5646 5678 4133
Size: 32 1481 32 4519
Fragments arranged by size:
4519 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3343 3343
Size: 6064
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6064
PsiI TTA'TAA
Cuts at: 3634 3634
Size: 6064
PstI C_TGCA'G
Cuts at: 830 2079 2232 3416 830
Size: 1249 153 1184 3478
Fragments arranged by size:
3478 1249 1184 153
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6064
PvuII CAG'CTG
Cuts at: 2243 2243
Size: 6064
SalI G'TCGA_C
Cuts at: 1936 3406 1936
Size: 1470 4594
SapI GCTCTTCn'nnn_
Cuts at: 2379 2648 2379
Size: 269 5795
SbfI CC_TGCA'GG
Cuts at: 2232 3416 2232
Size: 1184 4880
ScaI AGT'ACT
Cuts at: 4312 6024 4312
Size: 1712 4352
SexAI A'CCwGG_T
Cuts at: 2838 2838
Size: 6064
SfcI C'TryA_G
Cuts at: 826 1045 2075 2228 2257 3299 3412 4547 5228
Size: 219 1030 153 29 1042 113 1135 681
Cuts at: 5228 5419 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1042 1030 681 219 191 153
113 29
SfiI GGCCn_nnn'nGGCC
Cuts at: 2915 2915
Size: 6064
SfoI GGC'GCC
Cuts at: 2031 2031
Size: 6064
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6064
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6064
SmaI CCC'GGG
Cuts at: 1872 3391 1872
Size: 1519 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2272 3138 4168 5069 5331 820
Size: 197 926 329 866 1030 901 262 1553
Fragments arranged by size:
1553 1030 926 901 866 329 262 197
SnaBI TAC'GTA
Cuts at: 493 3361 3855 493
Size: 2868 494 2702
Fragments arranged by size:
2868 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6064
SphI G_CATG'C
Cuts at: 1132 2655 3422 1132
Size: 1523 767 3774
Fragments arranged by size:
3774 1523 767
SspI AAT'ATT
Cuts at: 5 52 3988 5
Size: 47 3936 2081
Fragments arranged by size:
3936 2081 47
StuI AGG'CCT
Cuts at: 1517 2775 2909 1517
Size: 1258 134 4672
Fragments arranged by size:
4672 1258 134
StyI C'CwwG_G
Cuts at: 513 1065 2022 2127 2285 3503 513
Size: 552 957 105 158 1218 3074
Fragments arranged by size:
3074 1218 957 552 158 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4091 4108 4261 4446 4891 325
Size: 1255 2511 17 153 185 445 1498
Fragments arranged by size:
2511 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2161 4310 6022
Size: 274 80 33 51 1443 184 2149 1712
Cuts at: 6022 96
Size: 138
Fragments arranged by size:
2149 1712 1443 274 184 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6024 6056 6024
Size: 32 6032
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6064
XbaI T'CTAG_A
Cuts at: 3400 3400
Size: 6064
XhoI C'TCGA_G
Cuts at: 1943 3138 1943
Size: 1195 4869
XmaI C'CCGG_G
Cuts at: 1870 3389 1870
Size: 1519 4545
XmnI GAAnn'nnTTC
Cuts at: 3222 4193 3222
Size: 971 5093
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3872 276
Size: 53 83 186 936 2338 2468
Fragments arranged by size:
2468 2338 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I EcoICRI EcoNI
EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SrfI SwaI XcmI