Restriction map for
pFN21ASDA0883
(Circular) (Six-base) MAPSORT of: pFN21ASDA0883.seq Check: 5419 from: 1 to: 5821
pFN21ASDA0883 5821 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3631 278
Size: 53 83 186 936 2095 2468
Fragments arranged by size:
2468 2095 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3164 1937
Size: 1227 4594
Acc65I G'GTAC_C
Cuts at: 3142 3142
Size: 5821
AclI AA'CG_TT
Cuts at: 1563 3948 4321 1563
Size: 2385 373 3063
Fragments arranged by size:
3063 2385 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2123 2807 2987 3884 5268 5536 1632
Size: 491 684 180 897 1384 268 1917
Fragments arranged by size:
1917 1384 897 684 491 268 180
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5624
AflIII A'CryG_T
Cuts at: 1203 4720 5697 1203
Size: 3517 977 1327
Fragments arranged by size:
3517 1327 977
AgeI A'CCGG_T
Cuts at: 4652 4652
Size: 5821
AhdI GACnn_n'nnGTC
Cuts at: 2698 4550 2698
Size: 1852 3969
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5821
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2220 1184
Size: 1036 4785
AlwNI CAG_nnn'CTG
Cuts at: 2491 2944 3564 5136 2491
Size: 453 620 1572 3176
Fragments arranged by size:
3176 1572 620 453
ApaI G_GGCC'C
Cuts at: 2660 2981 2660
Size: 321 5500
ApaLI G'TGCA_C
Cuts at: 3878 5034 3878
Size: 1156 4665
ApoI r'AATT_y
Cuts at: 1493 3130 3356 3508 5617 1493
Size: 1637 226 152 2109 1697
Fragments arranged by size:
2109 1697 1637 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4375 160
Size: 872 3343 1606
Fragments arranged by size:
3343 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
AvaI C'yCGr_G
Cuts at: 1870 1943 2374 3146 1870
Size: 73 431 772 4545
Fragments arranged by size:
4545 772 431 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2229 2262 2229
Size: 33 5788
BamHI G'GATC_C
Cuts at: 3151 3518 3151
Size: 367 5454
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2949 3142 4597 618
Size: 325 217 627 1162 193 1455 1842
Fragments arranged by size:
1842 1455 1162 627 325 217 193
BanII G_rGCy'C
Cuts at: 1391 2392 2660 2981 3140 1391
Size: 1001 268 321 159 4072
Fragments arranged by size:
4072 1001 321 268 159
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2795 928
Size: 971 896 3954
Fragments arranged by size:
3954 971 896
BbvCI CC'TCA_GC
Cuts at: 2484 2484
Size: 5821
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4012 4046 1903
Size: 34 2075 34 3678
Fragments arranged by size:
3678 2075 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2091 3713 4923 2091
Size: 1622 1210 2989
Fragments arranged by size:
2989 1622 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5821
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2655 4432 136
Size: 107 122 71 631 605 983 1777 1525
Fragments arranged by size:
1777 1525 983 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5816 5816
Size: 5821
BlpI GC'TnA_GC
Cuts at: 3238 3238
Size: 5821
Bme1580I G_kGCm'C
Cuts at: 1790 2427 2660 2981 3882 5038 1790
Size: 637 233 321 901 1156 2573
Fragments arranged by size:
2573 1156 901 637 321 233
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5821
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2253 3552 4510
Size: 416 53 83 399 24 836 1299 958
Cuts at: 4510 442
Size: 1753
Fragments arranged by size:
1753 1299 958 836 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5821
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2833 2833
Size: 5821
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2397 2927 4481 1239
Size: 1158 530 1554 2579
Fragments arranged by size:
2579 1554 1158 530
Bpu10I CC'TnA_GC
Cuts at: 2484 2957 2484
Size: 473 5348
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3017 3946 4811 5109 3017
Size: 929 865 298 3729
Fragments arranged by size:
3729 929 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2377 2821 4484 882
Size: 617 878 444 1663 2219
Fragments arranged by size:
2219 1663 878 617 444
BsaAI yAC'GTr
Cuts at: 493 1204 3118 3612 493
Size: 711 1914 494 2702
Fragments arranged by size:
2702 1914 711 494
BsaBI GATnn'nnATC
Cuts at: 5815 5815
Size: 5821
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3628 4010 4645 275
Size: 53 83 186 936 2095 382 635 1451
Fragments arranged by size:
2095 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2190 2508 2850 4254 4652 4926 5073 5484 2190
Size: 318 342 1404 398 274 147 411 2527
Fragments arranged by size:
2527 1404 411 398 342 318 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2393 2423 2799 2829 1178
Size: 30 165 30 990 30 376 30 4170
Fragments arranged by size:
4170 990 376 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2129 3041 1186
Size: 943 912 3966
Fragments arranged by size:
3966 943 912
BseYI C'CCAG_C
Cuts at: 2542 2921 2987 5024 5520 2542
Size: 379 66 2037 496 2843
Fragments arranged by size:
2843 2037 496 379 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2071 2705 1163
Size: 573 335 634 4279
Fragments arranged by size:
4279 634 573 335
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3271 4032 5060 1540
Size: 275 186 1270 761 1028 2301
Fragments arranged by size:
2301 1270 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3882 3967 5038 3882
Size: 85 1071 4665
Fragments arranged by size:
4665 1071 85
BsmI GAATG_Cn'
Cuts at: 2209 3332 3425 2209
Size: 1123 93 4605
Fragments arranged by size:
4605 1123 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3102 3102
Size: 5821
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2392 2427 2660 2981 3140 3882 3967
Size: 399 602 35 233 321 159 742 85
Cuts at: 3967 5038 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 602 399 321 233 159
85 35
BspEI T'CCGG_A
Cuts at: 2190 2850 5484 2190
Size: 660 2634 2527
Fragments arranged by size:
2634 2527 660
BspHI T'CATG_A
Cuts at: 3100 3710 5440 3100
Size: 610 1730 3481
Fragments arranged by size:
3481 1730 610
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2154 2902 3176 844
Size: 1310 748 274 3489
Fragments arranged by size:
3489 1310 748 274
BsrBI CCG'CTC
Cuts at: 1394 1823 2858 3708 1394
Size: 429 1035 850 3507
Fragments arranged by size:
3507 1035 850 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2475 4316 4490 65
Size: 1183 1227 1841 174 1396
Fragments arranged by size:
1841 1396 1227 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2101 2952 4465 4652 1700
Size: 110 291 851 1513 187 2869
Fragments arranged by size:
2869 1513 851 291 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5821
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5821
BssSI C'ACGA_G
Cuts at: 3881 4893 3881
Size: 1012 4809
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2683 794
Size: 1889 3932
BstBI TT'CG_AA
Cuts at: 2007 4625 2007
Size: 2618 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5821
BstXI CCAn_nnnn'nTGG
Cuts at: 2029 2029
Size: 5821
BstYI r'GATC_y
Cuts at: 1972 3151 3518 3903 3920 5372 5458 5470 5816
Size: 1179 367 385 17 1452 86 12 346
Cuts at: 5816 1972
Size: 1977
Fragments arranged by size:
1977 1452 1179 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2586 513
Size: 552 957 564 3748
Fragments arranged by size:
3748 957 564 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5082
Fragments arranged by size:
5082 683 56
BtsI GCAGTG_nn'
Cuts at: 3337 4131 4151 3337
Size: 794 20 5007
Fragments arranged by size:
5007 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5821
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5786
DraI TTT'AAA
Cuts at: 2151 3125 2151
Size: 974 4847
DraIII CAC_nnn'GTG
Cuts at: 2683 2683
Size: 5821
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2152 4828 809
Size: 507 836 2676 1802
Fragments arranged by size:
2676 1802 836 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2250 2606 3088
Size: 54 1425 50 275 18 420 356 482
Cuts at: 3088 3268 4157 5786 8
Size: 180 889 1629 43
Fragments arranged by size:
1629 1425 889 482 420 356 275 180
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3268 1537
Size: 275 1456 4090
Fragments arranged by size:
4090 1456 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2550 2709 3018 3027 3057 3751 1353
Size: 1197 159 309 9 30 694 3423
Fragments arranged by size:
3423 1197 694 309 159 30 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2120 2657 2891 4408 4782 4928
Size: 1064 138 531 537 234 1517 374 146
Cuts at: 4928 387
Size: 1280
Fragments arranged by size:
1517 1280 1064 537 531 374 234 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2123 2397 2807 2927 2987 3884 4481
Size: 393 491 274 410 120 60 897 597
Cuts at: 4481 5268 5536 1239
Size: 787 268 1524
Fragments arranged by size:
1524 897 787 597 491 410 393 274
268 120 60
EcoNI CCTnn'n_nnAGG
Cuts at: 2396 2909 2396
Size: 513 5308
EcoO109I rG'GnC_Cy
Cuts at: 2134 2656 2657 2977 2134
Size: 522 1 320 4978
Fragments arranged by size:
4978 522 320 1
EcoRI G'AATT_C
Cuts at: 3130 3508 3130
Size: 378 5443
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2314 2314
Size: 5821
FspI TGC'GCA
Cuts at: 4327 4327
Size: 5821
HaeII r_GCGC'y
Cuts at: 1678 2030 4968 1678
Size: 352 2938 2531
Fragments arranged by size:
2938 2531 352
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2063 2095 2296 2328 2393
Size: 32 135 32 491 32 201 32 65
Cuts at: 2393 2425 2543 2575 2684 2716 2799 2831 4462
Size: 32 118 32 109 32 83 32 1631
Cuts at: 4462 4494 4536 4568 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1631 491 201 135 118 109 83
65 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3165 3411 669
Size: 988 281 1227 246 3079
Fragments arranged by size:
3079 1227 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5821
HpaI GTT'AAC
Cuts at: 3411 3411
Size: 5821
KpnI G_GTAC'C
Cuts at: 3146 3146
Size: 5821
MfeI C'AATT_G
Cuts at: 3420 3420
Size: 5821
MluI A'CGCG_T
Cuts at: 5697 5697
Size: 5821
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2556 4935 5119 1305
Size: 1251 2379 184 2007
Fragments arranged by size:
2379 2007 1251 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2608 10
Size: 54 1425 343 776 3223
Fragments arranged by size:
3223 1425 776 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3779 4138 4297 518
Size: 648 2613 359 159 2042
Fragments arranged by size:
2613 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3093 3237 3914 5062 5307 1606
Size: 1487 144 677 1148 245 2120
Fragments arranged by size:
2120 1487 1148 677 245 144
NaeI GCC'GGC
Cuts at: 2954 2954
Size: 5821
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4312
Fragments arranged by size:
4312 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5821
NgoMIV G'CCGG_C
Cuts at: 2952 2952
Size: 5821
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5821
NotI GC'GGCC_GC
Cuts at: 3268 3268
Size: 5821
NspI r_CATG'y
Cuts at: 1132 1229 2480 3179 4724 1132
Size: 97 1251 699 1545 2229
Fragments arranged by size:
2229 1545 1251 699 97
PasI CC'CwG_GG
Cuts at: 1348 2392 1348
Size: 1044 4777
PciI A'CATG_T
Cuts at: 4720 4720
Size: 5821
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2458 1108
Size: 1350 4471
PmeI GTTT'AAAC
Cuts at: 3125 3125
Size: 5821
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3890 3922 5403 5435 3890
Size: 32 1481 32 4276
Fragments arranged by size:
4276 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2134 2134
Size: 5821
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5821
PsiI TTA'TAA
Cuts at: 3391 3391
Size: 5821
PspOMI G'GGCC_C
Cuts at: 2656 2977 2656
Size: 321 5500
PstI C_TGCA'G
Cuts at: 830 3173 830
Size: 2343 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5821
SalI G'TCGA_C
Cuts at: 1936 3163 1936
Size: 1227 4594
SapI GCTCTTCn'nnn_
Cuts at: 3057 3057
Size: 5821
SbfI CC_TGCA'GG
Cuts at: 3173 3173
Size: 5821
ScaI AGT'ACT
Cuts at: 4069 5781 4069
Size: 1712 4109
SexAI A'CCwGG_T
Cuts at: 2895 2895
Size: 5821
SfcI C'TryA_G
Cuts at: 826 1045 3169 4304 4985 5176 826
Size: 219 2124 1135 681 191 1471
Fragments arranged by size:
2124 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5821
SmaI CCC'GGG
Cuts at: 1872 3148 1872
Size: 1276 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2996 3925 4826 5088 820
Size: 197 926 1053 929 901 262 1553
Fragments arranged by size:
1553 1053 929 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3118 3612 493
Size: 2625 494 2702
Fragments arranged by size:
2702 2625 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5821
SphI G_CATG'C
Cuts at: 1132 3179 1132
Size: 2047 3774
SspI AAT'ATT
Cuts at: 5 52 3745 5
Size: 47 3693 2081
Fragments arranged by size:
3693 2081 47
StuI AGG'CCT
Cuts at: 1517 2729 3038 1517
Size: 1212 309 4300
Fragments arranged by size:
4300 1212 309
StyI C'CwwG_G
Cuts at: 513 1065 2022 3260 513
Size: 552 957 1238 3074
Fragments arranged by size:
3074 1238 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3848 3865 4018 4203 4648 325
Size: 1255 2268 17 153 185 445 1498
Fragments arranged by size:
2268 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4067 5779 96
Size: 274 80 33 51 1443 2090 1712 138
Fragments arranged by size:
2090 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2047 2079 5781 5813 2047
Size: 32 3702 32 2055
Fragments arranged by size:
3702 2055 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2897 1316
Size: 1581 4240
XbaI T'CTAG_A
Cuts at: 2350 3157 2350
Size: 807 5014
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5821
XmaI C'CCGG_G
Cuts at: 1870 3146 1870
Size: 1276 4545
XmnI GAAnn'nnTTC
Cuts at: 3950 3950
Size: 5821
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3629 276
Size: 53 83 186 936 2095 2468
Fragments arranged by size:
2468 2095 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BfrBI BsiWI
BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NarI
NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII
RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI
XcmI