Restriction map for
pFN21ASDA0880
(Circular) (Six-base) MAPSORT of: pFN21ASDA0880.seq Check: 3143 from: 1 to: 6856
pFN21ASDA0880 6856 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2992 2992
Size: 6856
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2282 4666 278
Size: 53 83 186 936 746 2384 2468
Fragments arranged by size:
2468 2384 936 746 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4199 1937
Size: 2262 4594
Acc65I G'GTAC_C
Cuts at: 2053 2062 4177 2053
Size: 9 2115 4732
Fragments arranged by size:
4732 2115 9
AclI AA'CG_TT
Cuts at: 1563 4983 5356 1563
Size: 3420 373 3063
Fragments arranged by size:
3420 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2930 3373 4919 6303 6571 1632
Size: 1298 443 1546 1384 268 1917
Fragments arranged by size:
1917 1546 1384 1298 443 268
AfeI AGC'GCT
Cuts at: 2258 2258
Size: 6856
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6659
AflIII A'CryG_T
Cuts at: 1203 5755 6732 1203
Size: 4552 977 1327
Fragments arranged by size:
4552 1327 977
AgeI A'CCGG_T
Cuts at: 3969 5687 3969
Size: 1718 5138
AhdI GACnn_n'nnGTC
Cuts at: 5585 5585
Size: 6856
AleI CACnn'nnGTG
Cuts at: 1166 3548 3899 1166
Size: 2382 351 4123
Fragments arranged by size:
4123 2382 351
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2372 2780 3680 3694 1184
Size: 1188 408 900 14 4346
Fragments arranged by size:
4346 1188 900 408 14
AlwNI CAG_nnn'CTG
Cuts at: 2578 2614 4007 4599 6171 2578
Size: 36 1393 592 1572 3263
Fragments arranged by size:
3263 1572 1393 592 36
ApaI G_GGCC'C
Cuts at: 3338 3913 3338
Size: 575 6281
ApaLI G'TGCA_C
Cuts at: 2357 3902 4913 6069 2357
Size: 1545 1011 1156 3144
Fragments arranged by size:
3144 1545 1156 1011
ApoI r'AATT_y
Cuts at: 1493 4165 4391 4543 6652 1493
Size: 2672 226 152 2109 1697
Fragments arranged by size:
2672 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5410 160
Size: 872 4378 1606
Fragments arranged by size:
4378 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6856
AvaI C'yCGr_G
Cuts at: 1870 1943 2702 4143 4181 1870
Size: 73 759 1441 38 4545
Fragments arranged by size:
4545 1441 759 73 38
BamHI G'GATC_C
Cuts at: 2706 3674 4186 4553 2706
Size: 968 512 367 5009
Fragments arranged by size:
5009 968 512 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2053 2062 2632 2843 3350
Size: 325 217 627 266 9 570 211 507
Cuts at: 3350 3706 4177 5632 618
Size: 356 471 1455 1842
Fragments arranged by size:
1842 1455 627 570 507 471 356 325
266 217 211 9
BanII G_rGCy'C
Cuts at: 1391 2237 3023 3338 3737 3913 4175 1391
Size: 846 786 315 399 176 262 4072
Fragments arranged by size:
4072 846 786 399 315 262 176
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3091 3807 928
Size: 971 1192 716 3977
Fragments arranged by size:
3977 1192 971 716
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2654 2688 3868 3902 5047 5081 1903
Size: 34 717 34 1180 34 1145 34 3678
Fragments arranged by size:
3678 1180 1145 717 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4748 5958 4748
Size: 1210 5646
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6856
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3576 5467 136
Size: 107 122 71 631 605 1904 1891 1525
Fragments arranged by size:
1904 1891 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6851 6851
Size: 6856
BlpI GC'TnA_GC
Cuts at: 4273 4273
Size: 6856
Bme1580I G_kGCm'C
Cuts at: 1790 2096 2361 2637 3338 3355 3460 3711 3906
Size: 306 265 276 701 17 105 251 195
Cuts at: 3906 3913 4917 6073 1790
Size: 7 1004 1156 2573
Fragments arranged by size:
2573 1156 1004 701 306 276 265 251
195 105 17 7
BmgBI CAC'GTC
Cuts at: 1318 2140 1318
Size: 822 6034
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3916 4587 5545
Size: 416 53 83 399 24 2499 671 958
Cuts at: 5545 442
Size: 1753
Fragments arranged by size:
2499 1753 958 671 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6856
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3517 3517
Size: 6856
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2130 2254 3236 3403 3491 3968 5516 1239
Size: 891 124 982 167 88 477 1548 2579
Fragments arranged by size:
2579 1548 982 891 477 167 124 88
Bpu10I CC'TnA_GC
Cuts at: 3914 3914
Size: 6856
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2539 3568 4981 5846 6144 2539
Size: 1029 1413 865 298 3251
Fragments arranged by size:
3251 1413 1029 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5519 882
Size: 617 4020 2219
Fragments arranged by size:
4020 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4153 4647 493
Size: 711 2949 494 2702
Fragments arranged by size:
2949 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 6850 6850
Size: 6856
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2279 3309 3925 4663
Size: 53 83 186 936 746 1030 616 738
Cuts at: 4663 5045 5680 275
Size: 382 635 1451
Fragments arranged by size:
1451 1030 936 746 738 635 616 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 3969 5289 5687 5961 6108 6519 3969
Size: 1320 398 274 147 411 4306
Fragments arranged by size:
4306 1320 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3209 3239 3749 3779 1178
Size: 30 165 30 1806 30 510 30 4255
Fragments arranged by size:
4255 1806 510 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3801 1186
Size: 2615 4241
BseYI C'CCAG_C
Cuts at: 2524 2571 2617 2752 2854 3364 3539 3588 3860
Size: 47 46 135 102 510 175 49 272
Cuts at: 3860 6059 6555 2524
Size: 2199 496 2825
Fragments arranged by size:
2825 2199 510 496 272 175 135 102
49 47 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2898 3712 1163
Size: 573 1162 814 4307
Fragments arranged by size:
4307 1162 814 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4306 5067 6095 1540
Size: 275 186 2305 761 1028 2301
Fragments arranged by size:
2305 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2237 2361 3906 4917 5002 6073 2237
Size: 124 1545 1011 85 1071 3020
Fragments arranged by size:
3020 1545 1071 1011 124 85
BsmI GAATG_Cn'
Cuts at: 4367 4460 4367
Size: 93 6763
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2096 2237 2361 2637 3023 3338 3355
Size: 399 306 141 124 276 386 315 17
Cuts at: 3355 3460 3711 3737 3906 3913 4175 4917 5002
Size: 105 251 26 169 7 262 742 85
Cuts at: 5002 6073 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 386 315 306 276
262 251 169 141 124 105 85 26
17 7
BspEI T'CCGG_A
Cuts at: 6519 6519
Size: 6856
BspHI T'CATG_A
Cuts at: 2211 2415 4745 6475 2211
Size: 204 2330 1730 2592
Fragments arranged by size:
2592 2330 1730 204
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2992 3261 3966 4211 844
Size: 2148 269 705 245 3489
Fragments arranged by size:
3489 2148 705 269 245
BsrBI CCG'CTC
Cuts at: 1394 1823 3400 4743 1394
Size: 429 1577 1343 3507
Fragments arranged by size:
3507 1577 1343 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5351 5525 65
Size: 1183 4103 174 1396
Fragments arranged by size:
4103 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2873 3969 5500 5687 1700
Size: 110 1063 1096 1531 187 2869
Fragments arranged by size:
2869 1531 1096 1063 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6856
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6856
BssSI C'ACGA_G
Cuts at: 3546 4916 5928 3546
Size: 1370 1012 4474
Fragments arranged by size:
4474 1370 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4105 794
Size: 3311 3545
BstBI TT'CG_AA
Cuts at: 2007 5660 2007
Size: 3653 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6856
BstXI CCAn_nnnn'nTGG
Cuts at: 3214 3214
Size: 6856
BstYI r'GATC_y
Cuts at: 1972 2706 3674 4079 4186 4553 4938 4955 6407
Size: 734 968 405 107 367 385 17 1452
Cuts at: 6407 6493 6505 6851 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 968 734 405 385 367 346
107 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2133 2469 3839 4040 2133
Size: 336 1370 201 4949
Fragments arranged by size:
4949 1370 336 201
BtgI C'CryG_G
Cuts at: 513 1065 2022 3177 3874 513
Size: 552 957 1155 697 3495
Fragments arranged by size:
3495 1155 957 697 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2851 487
Size: 683 56 1625 4492
Fragments arranged by size:
4492 1625 683 56
BtsI GCAGTG_nn'
Cuts at: 2724 2886 4372 5166 5186 2724
Size: 162 1486 794 20 4394
Fragments arranged by size:
4394 1486 794 162 20
ClaI AT'CG_AT
Cuts at: 1554 2664 1554
Size: 1110 5746
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3573 3608 529
Size: 35 3009 35 3777
Fragments arranged by size:
3777 3009 35 35
DraI TTT'AAA
Cuts at: 4160 4160
Size: 6856
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5863 809
Size: 507 4547 1802
Fragments arranged by size:
4547 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2144 3192 4303
Size: 54 1425 50 275 18 314 1048 1111
Cuts at: 4303 5192 6821 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1111 1048 889 314 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4303 1537
Size: 275 2491 4090
Fragments arranged by size:
4090 2491 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3389 3407 4786 1353
Size: 2036 18 1379 3423
Fragments arranged by size:
3423 2036 1379 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5443 5817 5963 387
Size: 1064 138 3854 374 146 1280
Fragments arranged by size:
3854 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2235 2235
Size: 6856
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2130 2254 2930 3236 3373 3403 3491
Size: 393 498 124 676 306 137 30 88
Cuts at: 3491 3968 4919 5516 6303 6571 1239
Size: 477 951 597 787 268 1524
Fragments arranged by size:
1524 951 787 676 597 498 477 393
306 268 137 124 88 30
EcoNI CCTnn'n_nnAGG
Cuts at: 3158 3158
Size: 6856
EcoO109I rG'GnC_Cy
Cuts at: 2027 2827 3335 3910 2027
Size: 800 508 575 4973
Fragments arranged by size:
4973 800 575 508
EcoRI G'AATT_C
Cuts at: 4165 4543 4165
Size: 378 6478
FspI TGC'GCA
Cuts at: 5362 5362
Size: 6856
HaeII r_GCGC'y
Cuts at: 1678 2260 3223 3885 6003 1678
Size: 582 963 662 2118 2531
Fragments arranged by size:
2531 2118 963 662 582
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2993 3025 4064 4096 5497
Size: 32 135 32 1421 32 1039 32 1401
Cuts at: 5497 5529 5571 5603 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1421 1401 1039 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4200 4446 669
Size: 988 281 2262 246 3079
Fragments arranged by size:
3079 2262 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6856
HpaI GTT'AAC
Cuts at: 4446 4446
Size: 6856
KpnI G_GTAC'C
Cuts at: 2057 2066 4181 2057
Size: 9 2115 4732
Fragments arranged by size:
4732 2115 9
MfeI C'AATT_G
Cuts at: 4103 4455 4103
Size: 352 6504
MluI A'CGCG_T
Cuts at: 6732 6732
Size: 6856
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5970 6154 1305
Size: 4665 184 2007
Fragments arranged by size:
4665 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3194 10
Size: 54 1425 343 1362 3672
Fragments arranged by size:
3672 1425 1362 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2580 2600 3299 3548 3899 4814 5173
Size: 648 1414 20 699 249 351 915 359
Cuts at: 5173 5332 518
Size: 159 2042
Fragments arranged by size:
2042 1414 915 699 648 359 351 249
159 20
MspA1I CmG'CkG
Cuts at: 1606 2045 2200 3642 4272 4949 6097 6342 1606
Size: 439 155 1442 630 677 1148 245 2120
Fragments arranged by size:
2120 1442 1148 677 630 439 245 155
NcoI C'CATG_G
Cuts at: 513 1065 2022 3177 513
Size: 552 957 1155 4192
Fragments arranged by size:
4192 1155 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6856
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6856
NotI GC'GGCC_GC
Cuts at: 4303 4303
Size: 6856
NspI r_CATG'y
Cuts at: 1132 1229 4214 5759 1132
Size: 97 2985 1545 2229
Fragments arranged by size:
2985 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3023 3105 3464 1348
Size: 1675 82 359 4740
Fragments arranged by size:
4740 1675 359 82
PciI A'CATG_T
Cuts at: 5755 5755
Size: 6856
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2804 3703 1108
Size: 1696 899 4261
Fragments arranged by size:
4261 1696 899
PfoI T'CCnGG_A
Cuts at: 3213 3597 3213
Size: 384 6472
PmeI GTTT'AAAC
Cuts at: 4160 4160
Size: 6856
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4925 4957 6438 6470 4925
Size: 32 1481 32 5311
Fragments arranged by size:
5311 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2027 2827 2027
Size: 800 6056
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6856
PsiI TTA'TAA
Cuts at: 4426 4426
Size: 6856
PspOMI G'GGCC_C
Cuts at: 3334 3909 3334
Size: 575 6281
PstI C_TGCA'G
Cuts at: 830 2199 3120 3641 4208 830
Size: 1369 921 521 567 3478
Fragments arranged by size:
3478 1369 921 567 521
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6856
PvuII CAG'CTG
Cuts at: 2200 3642 2200
Size: 1442 5414
SacI G_AGCT'C
Cuts at: 2237 2237
Size: 6856
SalI G'TCGA_C
Cuts at: 1936 4198 1936
Size: 2262 4594
SanDI GG'GwC_CC
Cuts at: 2027 2027
Size: 6856
SapI GCTCTTCn'nnn_
Cuts at: 3407 3407
Size: 6856
SbfI CC_TGCA'GG
Cuts at: 4208 4208
Size: 6856
ScaI AGT'ACT
Cuts at: 5104 6816 5104
Size: 1712 5144
SfcI C'TryA_G
Cuts at: 826 1045 2195 3116 3637 4204 5339 6020 6211
Size: 219 1150 921 521 567 1135 681 191
Cuts at: 6211 826
Size: 1471
Fragments arranged by size:
1471 1150 1135 921 681 567 521 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6856
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6856
SmaI CCC'GGG
Cuts at: 1872 4183 1872
Size: 2311 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2554 3583 4960 5861 6123 820
Size: 197 926 611 1029 1377 901 262 1553
Fragments arranged by size:
1553 1377 1029 926 901 611 262 197
SnaBI TAC'GTA
Cuts at: 493 4153 4647 493
Size: 3660 494 2702
Fragments arranged by size:
3660 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6856
SphI G_CATG'C
Cuts at: 1132 4214 1132
Size: 3082 3774
SspI AAT'ATT
Cuts at: 5 52 4780 5
Size: 47 4728 2081
Fragments arranged by size:
4728 2081 47
StuI AGG'CCT
Cuts at: 1517 2739 3497 1517
Size: 1222 758 4876
Fragments arranged by size:
4876 1222 758
StyI C'CwwG_G
Cuts at: 513 1065 2022 2916 2928 3177 4295 513
Size: 552 957 894 12 249 1118 3074
Fragments arranged by size:
3074 1118 957 894 552 249 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2827 2831 3271 3293 3580 3770 4883
Size: 1255 1247 4 440 22 287 190 1113
Cuts at: 4883 4900 5053 5238 5683 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1247 1113 445 440 287 190
185 153 22 17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5102 6814 96
Size: 274 80 33 51 1443 3125 1712 138
Fragments arranged by size:
3125 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3749 3781 6816 6848 3749
Size: 32 3035 32 3757
Fragments arranged by size:
3757 3035 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6856
XbaI T'CTAG_A
Cuts at: 4192 4192
Size: 6856
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2086 2245 3142 3547 2086
Size: 159 897 405 5395
Fragments arranged by size:
5395 897 405 159
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6856
XmaI C'CCGG_G
Cuts at: 1870 4181 1870
Size: 2311 4545
XmnI GAAnn'nnTTC
Cuts at: 2258 4985 2258
Size: 2727 4129
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2280 4664 276
Size: 53 83 186 936 746 2384 2468
Fragments arranged by size:
2468 2384 936 746 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI
BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI