Restriction map for
pFN21ASDA0876
(Circular) (Six-base) MAPSORT of: pFN21ASDA0876.seq Check: 5716 from: 1 to: 8017
pFN21ASDA0876 8017 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2233 4736 5272 2233
Size: 2503 536 4978
Fragments arranged by size:
4978 2503 536
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5827 278
Size: 53 83 186 936 4291 2468
Fragments arranged by size:
4291 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5360 1937
Size: 3423 4594
Acc65I G'GTAC_C
Cuts at: 5338 5338
Size: 8017
AclI AA'CG_TT
Cuts at: 1563 6144 6517 1563
Size: 4581 373 3063
Fragments arranged by size:
4581 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2779 2795 3137 6080 7464 7732 1632
Size: 1147 16 342 2943 1384 268 1917
Fragments arranged by size:
2943 1917 1384 1147 342 268 16
AfeI AGC'GCT
Cuts at: 3008 3219 3008
Size: 211 7806
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7820
AflIII A'CryG_T
Cuts at: 1203 2979 3654 4373 6916 7893 1203
Size: 1776 675 719 2543 977 1327
Fragments arranged by size:
2543 1776 1327 977 719 675
AgeI A'CCGG_T
Cuts at: 6848 6848
Size: 8017
AhdI GACnn_n'nnGTC
Cuts at: 6746 6746
Size: 8017
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8017
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4341 1184
Size: 3157 4860
AlwNI CAG_nnn'CTG
Cuts at: 3304 3503 4052 5760 7332 3304
Size: 199 549 1708 1572 3989
Fragments arranged by size:
3989 1708 1572 549 199
ApaI G_GGCC'C
Cuts at: 3497 3497
Size: 8017
ApaLI G'TGCA_C
Cuts at: 2941 6074 7230 2941
Size: 3133 1156 3728
Fragments arranged by size:
3728 3133 1156
ApoI r'AATT_y
Cuts at: 1493 2093 2822 5326 5552 5704 7813 1493
Size: 600 729 2504 226 152 2109 1697
Fragments arranged by size:
2504 2109 1697 729 600 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6571 160
Size: 872 5539 1606
Fragments arranged by size:
5539 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8017
AvaI C'yCGr_G
Cuts at: 1870 1943 2708 3002 3080 3105 3275 3789 4349
Size: 73 765 294 78 25 170 514 560
Cuts at: 4349 4496 4553 5173 5342 1870
Size: 147 57 620 169 4545
Fragments arranged by size:
4545 765 620 560 514 294 170 169
147 78 73 57 25
AvrII C'CTAG_G
Cuts at: 4783 4783
Size: 8017
BamHI G'GATC_C
Cuts at: 2787 4467 5347 5714 2787
Size: 1680 880 367 5090
Fragments arranged by size:
5090 1680 880 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2042 2230 2355 2519 3532
Size: 325 217 627 255 188 125 164 1013
Cuts at: 3532 3559 3616 3808 3855 4129 4613 4844 5152
Size: 27 57 192 47 274 484 231 308
Cuts at: 5152 5213 5338 6793 618
Size: 61 125 1455 1842
Fragments arranged by size:
1842 1455 1013 627 484 325 308 274
255 231 217 192 188 164 125 125
61 57 47 27
BanII G_rGCy'C
Cuts at: 1391 2143 2717 3092 3483 3497 3524 4068 4977
Size: 752 574 375 391 14 27 544 909
Cuts at: 4977 5166 5305 5336 1391
Size: 189 139 31 4072
Fragments arranged by size:
4072 909 752 574 544 391 375 189
139 31 27 14
BbeI G_GCGC'C
Cuts at: 2046 2234 3859 2046
Size: 188 1625 6204
Fragments arranged by size:
6204 1625 188
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2576 3186 4196 928
Size: 971 677 610 1010 4749
Fragments arranged by size:
4749 1010 971 677 610
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2509 2543 6208 6242 1903
Size: 34 572 34 3665 34 3678
Fragments arranged by size:
3678 3665 572 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4631 5909 7119 4631
Size: 1278 1210 5529
Fragments arranged by size:
5529 1278 1210
BclI T'GATC_A
Cuts at: 1548 2829 1548
Size: 1281 6736
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2147 2262 6628
Size: 107 122 71 631 605 475 115 4366
Cuts at: 6628 136
Size: 1525
Fragments arranged by size:
4366 1525 631 605 475 122 115 107
71
BglII A'GATC_T
Cuts at: 2967 8012 2967
Size: 5045 2972
BlpI GC'TnA_GC
Cuts at: 3086 5434 3086
Size: 2348 5669
Bme1580I G_kGCm'C
Cuts at: 1790 2945 3497 3552 3564 5155 5216 6078 7234
Size: 1155 552 55 12 1591 61 862 1156
Cuts at: 7234 1790
Size: 2573
Fragments arranged by size:
2573 1591 1156 1155 862 552 61 55
12
BmgBI CAC'GTC
Cuts at: 1318 2218 2458 2980 4374 4650 1318
Size: 900 240 522 1394 276 4685
Fragments arranged by size:
4685 1394 900 522 276 240
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2458 3558 5748
Size: 416 53 83 399 24 1041 1100 2190
Cuts at: 5748 6706 442
Size: 958 1753
Fragments arranged by size:
2190 1753 1100 1041 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8017
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3106 4660 3106
Size: 1554 6463
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2702 3115 3497 5120 6677 1239
Size: 1463 413 382 1623 1557 2579
Fragments arranged by size:
2579 1623 1557 1463 413 382
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3901 3997 4718 6142 7007 7305 3901
Size: 96 721 1424 865 298 4613
Fragments arranged by size:
4613 1424 865 721 298 96
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3144 3773 4723 6680 882
Size: 617 1645 629 950 1957 2219
Fragments arranged by size:
2219 1957 1645 950 629 617
BsaAI yAC'GTr
Cuts at: 493 1204 2116 2946 3613 3655 4474 4660 5257
Size: 711 912 830 667 42 819 186 597
Cuts at: 5257 5314 5808 493
Size: 57 494 2702
Fragments arranged by size:
2702 912 830 819 711 667 597 494
186 57 42
BsaBI GATnn'nnATC
Cuts at: 3839 8011 3839
Size: 4172 3845
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2043 2231 2730 3856
Size: 53 83 186 936 510 188 499 1126
Cuts at: 3856 3972 5824 6206 6841 275
Size: 116 1852 382 635 1451
Fragments arranged by size:
1852 1451 1126 936 635 510 499 382
188 186 116 83 53
BsaWI w'CCGG_w
Cuts at: 2484 3144 4977 6450 6848 7122 7269 7680 2484
Size: 660 1833 1473 398 274 147 411 2821
Fragments arranged by size:
2821 1833 1473 660 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2498 2528 2627 2657 3443
Size: 30 165 30 1095 30 99 30 786
Cuts at: 3443 3473 3641 3671 4871 4901 4964 4994 1178
Size: 30 168 30 1200 30 63 30 4201
Fragments arranged by size:
4201 1200 1095 786 168 165 99 63
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3119 3242 3266 3293 3296 3299 3302 3305
Size: 1933 123 24 27 3 3 3 3
Cuts at: 3305 3464 3530 3584 3941 4446 5008 5117 5120
Size: 159 66 54 357 505 562 109 3
Cuts at: 5120 5195 1186
Size: 75 4008
Fragments arranged by size:
4008 1933 562 505 357 159 123 109
75 66 54 27 24 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 2054 3209 3233 3257 3490 4942 5064 7220 7716
Size: 1155 24 24 233 1452 122 2156 496
Cuts at: 7716 2054
Size: 2355
Fragments arranged by size:
2355 2156 1452 1155 496 233 122 24
24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2609 3771 4066 5306 1163
Size: 573 873 1162 295 1240 3874
Fragments arranged by size:
3874 1240 1162 873 573 295
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3316 3626 3875 4199 5467 6228
Size: 275 186 1315 310 249 324 1268 761
Cuts at: 6228 7256 1540
Size: 1028 2301
Fragments arranged by size:
2301 1315 1268 1028 761 324 310 275
249 186
BsiHKAI G_wGCw'C
Cuts at: 2670 2717 2945 3092 3654 3681 4631 4641 4689
Size: 47 228 147 562 27 950 10 48
Cuts at: 4689 4977 5147 6078 6163 7234 2670
Size: 288 170 931 85 1071 3453
Fragments arranged by size:
3453 1071 950 931 562 288 228 170
147 85 48 47 27 10
BsmI GAATG_Cn'
Cuts at: 5528 5621 5528
Size: 93 7924
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2143 2670 2717 2945 3092 3483 3497
Size: 399 353 527 47 228 147 391 14
Cuts at: 3497 3524 3552 3564 3654 3681 4068 4631 4641
Size: 27 28 12 90 27 387 563 10
Cuts at: 4641 4689 4977 5147 5155 5166 5216 5305 5336
Size: 48 288 170 8 11 50 89 31
Cuts at: 5336 6078 6163 7234 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 563 527 399 391 387
353 288 228 170 147 90 89 85
50 48 47 31 28 27 27 14
12 11 10 8
BspEI T'CCGG_A
Cuts at: 2484 7680 2484
Size: 5196 2821
BspHI T'CATG_A
Cuts at: 2067 2826 5906 7636 2067
Size: 759 3080 1730 2448
Fragments arranged by size:
3080 2448 1730 759
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2233 2631 3126 3758 4309 4434 4673 4736
Size: 1389 398 495 632 551 125 239 63
Cuts at: 4736 4868 5272 5372 844
Size: 132 404 100 3489
Fragments arranged by size:
3489 1389 632 551 495 404 398 239
132 125 100 63
BsrBI CCG'CTC
Cuts at: 1394 1823 2686 3382 4201 5127 5904 1394
Size: 429 863 696 819 926 777 3507
Fragments arranged by size:
3507 926 863 819 777 696 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6512 6686 65
Size: 1183 5264 174 1396
Fragments arranged by size:
5264 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2855 3529 3852 3870 5292 6661 6848
Size: 110 1045 674 323 18 1422 1369 187
Cuts at: 6848 1700
Size: 2869
Fragments arranged by size:
2869 1422 1369 1045 674 323 187 110
18
BsrGI T'GTAC_A
Cuts at: 96 2607 96
Size: 2511 5506
BssHII G'CGCG_C
Cuts at: 1925 4055 4502 1925
Size: 2130 447 5440
Fragments arranged by size:
5440 2130 447
BssSI C'ACGA_G
Cuts at: 4921 6077 7089 4921
Size: 1156 1012 5849
Fragments arranged by size:
5849 1156 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3304 4052 4311 794
Size: 2510 748 259 4500
Fragments arranged by size:
4500 2510 748 259
BstBI TT'CG_AA
Cuts at: 2007 6821 2007
Size: 4814 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8017
BstXI CCAn_nnnn'nTGG
Cuts at: 2305 2305
Size: 8017
BstYI r'GATC_y
Cuts at: 1972 2787 2967 4467 5347 5714 6099 6116 7568
Size: 815 180 1500 880 367 385 17 1452
Cuts at: 7568 7654 7666 8012 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1500 1452 880 815 385 367 346
180 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3555 4769 3555
Size: 1214 6803
BtgI C'CryG_G
Cuts at: 513 1065 2022 2038 2085 2189 2351 2973 3063
Size: 552 957 16 47 104 162 622 90
Cuts at: 3063 3510 3620 3723 5226 513
Size: 447 110 103 1503 3304
Fragments arranged by size:
3304 1503 957 622 552 447 162 110
104 103 90 47 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4471 487
Size: 683 56 3245 4033
Fragments arranged by size:
4033 3245 683 56
BtsI GCAGTG_nn'
Cuts at: 3302 3766 4004 4639 5533 6327 6347 3302
Size: 464 238 635 894 794 20 4972
Fragments arranged by size:
4972 894 794 635 464 238 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8017
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7982
DraI TTT'AAA
Cuts at: 2098 5321 2098
Size: 3223 4794
DraIII CAC_nnn'GTG
Cuts at: 2940 4534 2940
Size: 1594 6423
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2257 7024 809
Size: 507 941 4767 1802
Fragments arranged by size:
4767 1802 941 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2157 2325 2691
Size: 54 1425 50 275 18 327 168 366
Cuts at: 2691 2931 3313 3359 3623 3872 4014 4161 5464
Size: 240 382 46 264 249 142 147 1303
Cuts at: 5464 6353 7982 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1303 889 382 366 327 275
264 249 240 168 147 142 54 50
46 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3313 3623 3872 5464 1537
Size: 275 1501 310 249 1592 4090
Fragments arranged by size:
4090 1592 1501 310 275 249
EarI CTCTTCn'nnn_
Cuts at: 1353 2273 3330 3386 3842 4094 5947 1353
Size: 920 1057 56 456 252 1853 3423
Fragments arranged by size:
3423 1853 1057 920 456 252 56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5257 6604 6978 7124 387
Size: 1064 138 3668 1347 374 146 1280
Fragments arranged by size:
3668 1347 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2715 3090 4975 2715
Size: 375 1885 5757
Fragments arranged by size:
5757 1885 375
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2702 2779 2795 3115 3137 3497 5120
Size: 393 1070 77 16 320 22 360 1623
Cuts at: 5120 6080 6677 7464 7732 1239
Size: 960 597 787 268 1524
Fragments arranged by size:
1623 1524 1070 960 787 597 393 360
320 268 77 22 16
EcoNI CCTnn'n_nnAGG
Cuts at: 3227 4148 4787 5000 5102 5279 3227
Size: 921 639 213 102 177 5965
Fragments arranged by size:
5965 921 639 213 177 102
EcoO109I rG'GnC_Cy
Cuts at: 3034 3500 4108 4946 5061 3034
Size: 466 608 838 115 5990
Fragments arranged by size:
5990 838 608 466 115
EcoRI G'AATT_C
Cuts at: 2822 5326 5704 2822
Size: 2504 378 5135
Fragments arranged by size:
5135 2504 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2593 3412 3655 2593
Size: 819 243 6955
Fragments arranged by size:
6955 819 243
FseI GG_CCGG'CC
Cuts at: 3874 5296 3874
Size: 1422 6595
FspI TGC'GCA
Cuts at: 2881 2990 6523 2881
Size: 109 3533 4375
Fragments arranged by size:
4375 3533 109
FspAI rTGC'GCAy
Cuts at: 2990 2990
Size: 8017
HaeII r_GCGC'y
Cuts at: 1678 2046 2234 2680 3010 3071 3221 3458 3859
Size: 368 188 446 330 61 150 237 401
Cuts at: 3859 4528 4548 5203 7164 1678
Size: 669 20 655 1961 2531
Fragments arranged by size:
2531 1961 669 655 446 401 368 330
237 188 150 61 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2627 2659 3641 3673 3917
Size: 32 135 32 1055 32 982 32 244
Cuts at: 3917 3949 4188 4220 4871 4903 5045 5077 6658
Size: 32 239 32 651 32 142 32 1581
Cuts at: 6658 6690 6732 6764 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1581 1055 982 651 244 239 142
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3271 5361 5607 669
Size: 988 281 1333 2090 246 3079
Fragments arranged by size:
3079 2090 1333 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8017
HpaI GTT'AAC
Cuts at: 5607 5607
Size: 8017
KasI G'GCGC_C
Cuts at: 2042 2230 3855 2042
Size: 188 1625 6204
Fragments arranged by size:
6204 1625 188
KpnI G_GTAC'C
Cuts at: 5342 5342
Size: 8017
MfeI C'AATT_G
Cuts at: 5616 5616
Size: 8017
MluI A'CGCG_T
Cuts at: 7893 7893
Size: 8017
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3722 4786 7131 7315 1305
Size: 2417 1064 2345 184 2007
Fragments arranged by size:
2417 2345 2007 1064 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2159 2327 2693 2933 10
Size: 54 1425 343 327 168 366 240 5094
Fragments arranged by size:
5094 1425 366 343 327 240 168 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2303 2978 3602 5975 6334 6493 518
Size: 648 1137 675 624 2373 359 159 2042
Fragments arranged by size:
2373 2042 1137 675 648 624 359 159
MspA1I CmG'CkG
Cuts at: 1606 2058 2191 2353 2436 3418 3622 3985 4049
Size: 452 133 162 83 982 204 363 64
Cuts at: 4049 4249 4561 4830 4942 5068 5433 6110 7258
Size: 200 312 269 112 126 365 677 1148
Cuts at: 7258 7503 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 982 677 452 365 363 312
269 245 204 200 162 133 126 112
83 64
NaeI GCC'GGC
Cuts at: 2857 3531 3854 3872 5294 2857
Size: 674 323 18 1422 5580
Fragments arranged by size:
5580 1422 674 323 18
NarI GG'CG_CC
Cuts at: 2043 2231 3856 2043
Size: 188 1625 6204
Fragments arranged by size:
6204 1625 188
NcoI C'CATG_G
Cuts at: 513 1065 2022 2085 2973 3510 3723 513
Size: 552 957 63 888 537 213 4807
Fragments arranged by size:
4807 957 888 552 537 213 63
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8017
NgoMIV G'CCGG_C
Cuts at: 2855 3529 3852 3870 5292 2855
Size: 674 323 18 1422 5580
Fragments arranged by size:
5580 1422 674 323 18
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8017
NotI GC'GGCC_GC
Cuts at: 5464 5464
Size: 8017
NspI r_CATG'y
Cuts at: 1132 1229 2566 5375 6920 1132
Size: 97 1337 2809 1545 2229
Fragments arranged by size:
2809 2229 1545 1337 97
PasI CC'CwG_GG
Cuts at: 1348 3194 3865 1348
Size: 1846 671 5500
Fragments arranged by size:
5500 1846 671
PciI A'CATG_T
Cuts at: 6916 6916
Size: 8017
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2245 3865 4534 1108
Size: 1137 1620 669 4591
Fragments arranged by size:
4591 1620 1137 669
PfoI T'CCnGG_A
Cuts at: 2493 3092 2493
Size: 599 7418
PmeI GTTT'AAAC
Cuts at: 5321 5321
Size: 8017
PmlI CAC'GTG
Cuts at: 2946 3655 4474 4660 2946
Size: 709 819 186 6303
Fragments arranged by size:
6303 819 709 186
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4022 4054 6086 6118 7599 7631 4022
Size: 32 2032 32 1481 32 4408
Fragments arranged by size:
4408 2032 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3034 4946 5061 3034
Size: 1912 115 5990
Fragments arranged by size:
5990 1912 115
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8017
PsiI TTA'TAA
Cuts at: 5587 5587
Size: 8017
PspOMI G'GGCC_C
Cuts at: 3493 3493
Size: 8017
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2531 2563 2531
Size: 32 7985
PstI C_TGCA'G
Cuts at: 830 3000 3508 3999 4320 4446 5283 5369 830
Size: 2170 508 491 321 126 837 86 3478
Fragments arranged by size:
3478 2170 837 508 491 321 126 86
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8017
PvuII CAG'CTG
Cuts at: 2058 3418 4942 5068 2058
Size: 1360 1524 126 5007
Fragments arranged by size:
5007 1524 1360 126
SacI G_AGCT'C
Cuts at: 2717 3092 4977 2717
Size: 375 1885 5757
Fragments arranged by size:
5757 1885 375
SacII CC_GC'GG
Cuts at: 2192 2354 3623 2192
Size: 162 1269 6586
Fragments arranged by size:
6586 1269 162
SalI G'TCGA_C
Cuts at: 1936 5359 1936
Size: 3423 4594
SanDI GG'GwC_CC
Cuts at: 4946 5061 4946
Size: 115 7902
SapI GCTCTTCn'nnn_
Cuts at: 3386 3842 4094 3386
Size: 456 252 7309
Fragments arranged by size:
7309 456 252
SbfI CC_TGCA'GG
Cuts at: 4320 5283 5369 4320
Size: 963 86 6968
Fragments arranged by size:
6968 963 86
ScaI AGT'ACT
Cuts at: 2342 6265 7977 2342
Size: 3923 1712 2382
Fragments arranged by size:
3923 2382 1712
SexAI A'CCwGG_T
Cuts at: 5040 5040
Size: 8017
SfcI C'TryA_G
Cuts at: 826 1045 2996 3504 3995 4112 4316 4442 4891
Size: 219 1951 508 491 117 204 126 449
Cuts at: 4891 5279 5365 6500 7181 7372 826
Size: 388 86 1135 681 191 1471
Fragments arranged by size:
1951 1471 1135 681 508 491 449 388
219 204 191 126 117 86
SfiI GGCCn_nnn'nGGCC
Cuts at: 2262 2262
Size: 8017
SfoI GGC'GCC
Cuts at: 2044 2232 3857 2044
Size: 188 1625 6204
Fragments arranged by size:
6204 1625 188
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8017
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8017
SmaI CCC'GGG
Cuts at: 1872 2710 3107 3791 5344 1872
Size: 838 397 684 1553 4545
Fragments arranged by size:
4545 1553 838 684 397
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3916 3976 4733 6121 7022 7284
Size: 197 926 1973 60 757 1388 901 262
Cuts at: 7284 820
Size: 1553
Fragments arranged by size:
1973 1553 1388 926 901 757 262 197
60
SnaBI TAC'GTA
Cuts at: 493 5314 5808 493
Size: 4821 494 2702
Fragments arranged by size:
4821 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8017
SphI G_CATG'C
Cuts at: 1132 5375 1132
Size: 4243 3774
SrfI GCCC'GGGC
Cuts at: 3791 3791
Size: 8017
SspI AAT'ATT
Cuts at: 5 52 5941 5
Size: 47 5889 2081
Fragments arranged by size:
5889 2081 47
StuI AGG'CCT
Cuts at: 1517 3671 3914 1517
Size: 2154 243 5620
Fragments arranged by size:
5620 2154 243
StyI C'CwwG_G
Cuts at: 513 1065 2022 2085 2973 3366 3510 3723 4783
Size: 552 957 63 888 393 144 213 1060
Cuts at: 4783 5187 5456 513
Size: 404 269 3074
Fragments arranged by size:
3074 1060 957 888 552 404 393 269
213 144 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4147 6044 6061 6214 6399 6844 325
Size: 1255 2567 1897 17 153 185 445 1498
Fragments arranged by size:
2567 1897 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2277 2340 2553
Size: 274 80 33 51 1443 300 63 213
Cuts at: 2553 2607 4581 4821 6263 7975 96
Size: 54 1974 240 1442 1712 138
Fragments arranged by size:
1974 1712 1443 1442 300 274 240 213
138 80 63 54 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2498 2530 3443 3475 4964 4996 7977 8009 2498
Size: 32 913 32 1489 32 2981 32 2506
Fragments arranged by size:
2981 2506 1489 913 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2960 4934 1316
Size: 1644 1974 4399
Fragments arranged by size:
4399 1974 1644
XbaI T'CTAG_A
Cuts at: 5353 5353
Size: 8017
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2242 2497 3610 3832 2242
Size: 255 1113 222 6427
Fragments arranged by size:
6427 1113 255 222
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8017
XmaI C'CCGG_G
Cuts at: 1870 2708 3105 3789 5342 1870
Size: 838 397 684 1553 4545
Fragments arranged by size:
4545 1553 838 684 397
XmnI GAAnn'nnTTC
Cuts at: 3395 6146 3395
Size: 2751 5266
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5825 276
Size: 53 83 186 936 4291 2468
Fragments arranged by size:
4291 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI
BstZ17I EcoRV NruI NsiI PacI PspXI RsrII SwaI