Restriction map for
pFN21ASDA0875
(Circular) (Six-base) MAPSORT of: pFN21ASDA0875.seq Check: 1312 from: 1 to: 6592
pFN21ASDA0875 6592 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2204 2750 3587 2204
Size: 546 837 5209
Fragments arranged by size:
5209 837 546
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4402 278
Size: 53 83 186 936 2866 2468
Fragments arranged by size:
2866 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2040 3935 1937
Size: 103 1895 4594
Fragments arranged by size:
4594 1895 103
Acc65I G'GTAC_C
Cuts at: 3913 3913
Size: 6592
AclI AA'CG_TT
Cuts at: 1563 4719 5092 1563
Size: 3156 373 3063
Fragments arranged by size:
3156 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2398 2564 2861 4655 6039 6307 1632
Size: 766 166 297 1794 1384 268 1917
Fragments arranged by size:
1917 1794 1384 766 297 268 166
AflII C'TTAA_G
Cuts at: 820 1017 2567 820
Size: 197 1550 4845
Fragments arranged by size:
4845 1550 197
AflIII A'CryG_T
Cuts at: 1203 5491 6468 1203
Size: 4288 977 1327
Fragments arranged by size:
4288 1327 977
AgeI A'CCGG_T
Cuts at: 5423 5423
Size: 6592
AhdI GACnn_n'nnGTC
Cuts at: 2599 5321 2599
Size: 2722 3870
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6592
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2954 3153 1184
Size: 1770 199 4623
Fragments arranged by size:
4623 1770 199
AlwNI CAG_nnn'CTG
Cuts at: 2196 2806 3244 4335 5907 2196
Size: 610 438 1091 1572 2881
Fragments arranged by size:
2881 1572 1091 610 438
ApaLI G'TGCA_C
Cuts at: 4649 5805 4649
Size: 1156 5436
ApoI r'AATT_y
Cuts at: 1493 2526 3901 4127 4279 6388 1493
Size: 1033 1375 226 152 2109 1697
Fragments arranged by size:
2109 1697 1375 1033 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5146 160
Size: 872 4114 1606
Fragments arranged by size:
4114 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6592
AvaI C'yCGr_G
Cuts at: 1870 1943 3385 3917 1870
Size: 73 1442 532 4545
Fragments arranged by size:
4545 1442 532 73
BamHI G'GATC_C
Cuts at: 3600 3922 4289 3600
Size: 322 367 5903
Fragments arranged by size:
5903 367 322
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2059 3018 3913 5368 618
Size: 325 217 627 272 959 895 1455 1842
Fragments arranged by size:
1842 1455 959 895 627 325 272 217
BanII G_rGCy'C
Cuts at: 1391 3522 3911 1391
Size: 2131 389 4072
Fragments arranged by size:
4072 2131 389
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5621
BbvCI CC'TCA_GC
Cuts at: 2102 2102
Size: 6592
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4783 4817 1903
Size: 34 2846 34 3678
Fragments arranged by size:
3678 2846 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2483 3822 4484 5694 2483
Size: 1339 662 1210 3381
Fragments arranged by size:
3381 1339 1210 662
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6592
BfrBI ATG'CAT
Cuts at: 2893 2893
Size: 6592
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2756 5203 136
Size: 107 122 71 631 605 1084 2447 1525
Fragments arranged by size:
2447 1525 1084 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2553 3820 6587 2553
Size: 1267 2767 2558
Fragments arranged by size:
2767 2558 1267
BlpI GC'TnA_GC
Cuts at: 4009 4009
Size: 6592
Bme1580I G_kGCm'C
Cuts at: 1790 4653 5809 1790
Size: 2863 1156 2573
Fragments arranged by size:
2863 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3745 1318
Size: 2427 4165
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4323 5281 442
Size: 416 53 83 399 24 2906 958 1753
Fragments arranged by size:
2906 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6592
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3691 3691
Size: 6592
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2173 2777 2999 3347 3821 3845 5252
Size: 917 17 604 222 348 474 24 1407
Cuts at: 5252 1239
Size: 2579
Fragments arranged by size:
2579 1407 917 604 474 348 222 24
17
Bpu10I CC'TnA_GC
Cuts at: 2102 2102
Size: 6592
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3708 4717 5582 5880 3708
Size: 1009 865 298 4420
Fragments arranged by size:
4420 1009 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5255 882
Size: 617 3756 2219
Fragments arranged by size:
3756 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3133 3889 4383 493
Size: 711 1929 756 494 2702
Fragments arranged by size:
2702 1929 756 711 494
BsaBI GATnn'nnATC
Cuts at: 3264 6586 3264
Size: 3322 3270
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4399 4781 5416 275
Size: 53 83 186 936 2866 382 635 1451
Fragments arranged by size:
2866 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3603 3807 5025 5423 5697 5844 6255 3603
Size: 204 1218 398 274 147 411 3940
Fragments arranged by size:
3940 1218 411 398 274 204 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3794 3824 1178
Size: 30 165 30 2391 30 3946
Fragments arranged by size:
3946 2391 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2090 3290 3293 3467 1186
Size: 904 1200 3 174 4311
Fragments arranged by size:
4311 1200 904 174 3
BseYI C'CCAG_C
Cuts at: 2339 2741 3567 5795 6291 2339
Size: 402 826 2228 496 2640
Fragments arranged by size:
2640 2228 826 496 402
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3311 3437 3866 1163
Size: 573 1575 126 429 3889
Fragments arranged by size:
3889 1575 573 429 126
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2169 2182 4042 4803 5831 1540
Size: 275 186 168 13 1860 761 1028 2301
Fragments arranged by size:
2301 1860 1028 761 275 186 168 13
BsiHKAI G_wGCw'C
Cuts at: 2397 3435 4653 4738 5809 2397
Size: 1038 1218 85 1071 3180
Fragments arranged by size:
3180 1218 1071 1038 85
BsmI GAATG_Cn'
Cuts at: 3113 4103 4196 3113
Size: 990 93 5509
Fragments arranged by size:
5509 990 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2397 3435 3522 3911 4653 4738 5809
Size: 399 607 1038 87 389 742 85 1071
Cuts at: 5809 1391
Size: 2174
Fragments arranged by size:
2174 1071 1038 742 607 399 389 87
85
BspEI T'CCGG_A
Cuts at: 3603 6255 3603
Size: 2652 3940
BspHI T'CATG_A
Cuts at: 4481 6211 4481
Size: 1730 4862
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2127 2204 2547 2750 3587 3947 844
Size: 1283 77 343 203 837 360 3489
Fragments arranged by size:
3489 1283 837 360 343 203 77
BsrBI CCG'CTC
Cuts at: 1394 1823 4479 1394
Size: 429 2656 3507
Fragments arranged by size:
3507 2656 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3279 5087 5261 65
Size: 1183 2031 1808 174 1396
Fragments arranged by size:
2031 1808 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2062 2199 5236 5423 1700
Size: 110 252 137 3037 187 2869
Fragments arranged by size:
3037 2869 252 187 137 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6592
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6592
BssSI C'ACGA_G
Cuts at: 3593 4652 5664 3593
Size: 1059 1012 4521
Fragments arranged by size:
4521 1059 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6592
BstBI TT'CG_AA
Cuts at: 2007 5396 2007
Size: 3389 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6592
BstXI CCAn_nnnn'nTGG
Cuts at: 2779 2779
Size: 6592
BstYI r'GATC_y
Cuts at: 1972 2553 3259 3600 3804 3820 3922 4289 4674
Size: 581 706 341 204 16 102 367 385
Cuts at: 4674 4691 6143 6229 6241 6587 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 706 581 385 367 346 341
204 102 86 17 16 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5083
Fragments arranged by size:
5083 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2063 487
Size: 683 56 837 5016
Fragments arranged by size:
5016 837 683 56
BtsI GCAGTG_nn'
Cuts at: 4108 4902 4922 4108
Size: 794 20 5778
Fragments arranged by size:
5778 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6592
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6557
DraI TTT'AAA
Cuts at: 3896 3896
Size: 6592
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5599 809
Size: 507 4283 1802
Fragments arranged by size:
4283 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2166 2179 3124
Size: 54 1425 50 275 18 336 13 945
Cuts at: 3124 3338 3634 4039 4928 6557 8
Size: 214 296 405 889 1629 43
Fragments arranged by size:
1629 1425 945 889 405 336 296 275
214 54 50 43 18 13
EagI C'GGCC_G
Cuts at: 1537 1812 2166 2179 4039 1537
Size: 275 354 13 1860 4090
Fragments arranged by size:
4090 1860 354 275 13
EarI CTCTTCn'nnn_
Cuts at: 1353 2310 4522 1353
Size: 957 2212 3423
Fragments arranged by size:
3423 2212 957
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2087 5179 5553 5699 387
Size: 1064 138 498 3092 374 146 1280
Fragments arranged by size:
3092 1280 1064 498 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2173 2398 2564 2777 2861 2999
Size: 393 524 17 225 166 213 84 138
Cuts at: 2999 3347 3821 3845 4655 5252 6039 6307 1239
Size: 348 474 24 810 597 787 268 1524
Fragments arranged by size:
1524 810 787 597 524 474 393 348
268 225 213 166 138 84 24 17
EcoO109I rG'GnC_Cy
Cuts at: 3571 3571
Size: 6592
EcoRI G'AATT_C
Cuts at: 3901 4279 3901
Size: 378 6214
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2401 2514 3332 2401
Size: 113 818 5661
Fragments arranged by size:
5661 818 113
FspI TGC'GCA
Cuts at: 5098 5098
Size: 6592
HaeII r_GCGC'y
Cuts at: 1678 2069 5739 1678
Size: 391 3670 2531
Fragments arranged by size:
3670 2531 391
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2585 2617 2941 2973 3796
Size: 32 135 32 1013 32 324 32 823
Cuts at: 3796 3828 5233 5265 5307 5339 1373
Size: 32 1405 32 42 32 2626
Fragments arranged by size:
2626 1405 1013 823 324 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2041 2116 2989 3936 4182 669
Size: 988 281 103 75 873 947 246 3079
Fragments arranged by size:
3079 988 947 873 281 246 103 75
HindIII A'AGCT_T
Cuts at: 748 2496 748
Size: 1748 4844
HpaI GTT'AAC
Cuts at: 4182 4182
Size: 6592
KpnI G_GTAC'C
Cuts at: 3917 3917
Size: 6592
MfeI C'AATT_G
Cuts at: 2305 2952 4191 2305
Size: 647 1239 4706
Fragments arranged by size:
4706 1239 647
MluI A'CGCG_T
Cuts at: 6468 6468
Size: 6592
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2292 2676 5706 5890 1305
Size: 987 384 3030 184 2007
Fragments arranged by size:
3030 2007 987 384 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3340 10
Size: 54 1425 343 1508 3262
Fragments arranged by size:
3262 1508 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2777 3498 3581 4550 4909 5068 518
Size: 648 1611 721 83 969 359 159 2042
Fragments arranged by size:
2042 1611 969 721 648 359 159 83
MspA1I CmG'CkG
Cuts at: 1606 2106 2229 3094 4008 4685 5833 6078 1606
Size: 500 123 865 914 677 1148 245 2120
Fragments arranged by size:
2120 1148 914 865 677 500 245 123
NaeI GCC'GGC
Cuts at: 2064 2201 2064
Size: 137 6455
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5083
Fragments arranged by size:
5083 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6592
NgoMIV G'CCGG_C
Cuts at: 2062 2199 2062
Size: 137 6455
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6592
NotI GC'GGCC_GC
Cuts at: 4039 4039
Size: 6592
NsiI A_TGCA'T
Cuts at: 2895 2895
Size: 6592
NspI r_CATG'y
Cuts at: 1132 1229 2935 3950 5495 1132
Size: 97 1706 1015 1545 2229
Fragments arranged by size:
2229 1706 1545 1015 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6592
PciI A'CATG_T
Cuts at: 5491 5491
Size: 6592
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2806 1108
Size: 1698 4894
PmeI GTTT'AAAC
Cuts at: 3896 3896
Size: 6592
PmlI CAC'GTG
Cuts at: 3133 3133
Size: 6592
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4661 4693 6174 6206 4661
Size: 32 1481 32 5047
Fragments arranged by size:
5047 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3571 3571
Size: 6592
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6592
PsiI TTA'TAA
Cuts at: 4162 4162
Size: 6592
PstI C_TGCA'G
Cuts at: 830 3142 3944 830
Size: 2312 802 3478
Fragments arranged by size:
3478 2312 802
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6592
PvuII CAG'CTG
Cuts at: 2106 3094 2106
Size: 988 5604
SalI G'TCGA_C
Cuts at: 1936 2039 3934 1936
Size: 103 1895 4594
Fragments arranged by size:
4594 1895 103
SanDI GG'GwC_CC
Cuts at: 3571 3571
Size: 6592
SbfI CC_TGCA'GG
Cuts at: 3944 3944
Size: 6592
ScaI AGT'ACT
Cuts at: 2636 3116 4840 6552 2636
Size: 480 1724 1712 2676
Fragments arranged by size:
2676 1724 1712 480
SexAI A'CCwGG_T
Cuts at: 3285 3411 3285
Size: 126 6466
SfcI C'TryA_G
Cuts at: 826 1045 2284 3138 3940 5075 5756 5947 826
Size: 219 1239 854 802 1135 681 191 1471
Fragments arranged by size:
1471 1239 1135 854 802 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6592
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6592
SmaI CCC'GGG
Cuts at: 1872 3387 3919 1872
Size: 1515 532 4545
Fragments arranged by size:
4545 1515 532
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2567 3723 4696 5597 5859 820
Size: 197 926 624 1156 973 901 262 1553
Fragments arranged by size:
1553 1156 973 926 901 624 262 197
SnaBI TAC'GTA
Cuts at: 493 3889 4383 493
Size: 3396 494 2702
Fragments arranged by size:
3396 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6592
SphI G_CATG'C
Cuts at: 1132 3950 1132
Size: 2818 3774
SspI AAT'ATT
Cuts at: 5 52 3853 4516 5
Size: 47 3801 663 2081
Fragments arranged by size:
3801 2081 663 47
StuI AGG'CCT
Cuts at: 1517 2573 1517
Size: 1056 5536
StyI C'CwwG_G
Cuts at: 513 1065 2022 3022 4031 513
Size: 552 957 1000 1009 3074
Fragments arranged by size:
3074 1009 1000 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4619 4636 4789 4974 5419 325
Size: 1255 3039 17 153 185 445 1498
Fragments arranged by size:
3039 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2139 2634 3114
Size: 274 80 33 51 1443 162 495 480
Cuts at: 3114 4838 6550 96
Size: 1724 1712 138
Fragments arranged by size:
1724 1712 1443 495 480 274 162 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6552 6584 6552
Size: 32 6560
Tth111I GACn'n_nGTC
Cuts at: 1316 2300 1316
Size: 984 5608
XbaI T'CTAG_A
Cuts at: 3427 3928 3427
Size: 501 6091
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3583 3583
Size: 6592
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6592
XmaI C'CCGG_G
Cuts at: 1870 3385 3917 1870
Size: 1515 532 4545
Fragments arranged by size:
4545 1515 532
XmnI GAAnn'nnTTC
Cuts at: 4721 4721
Size: 6592
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4400 276
Size: 53 83 186 936 2866 2468
Fragments arranged by size:
2866 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI
FspAI KasI NarI NruI PacI PfoI PspOMI PspXI
PsrI RsrII SacI SacII SapI SfiI SfoI SrfI
SwaI