Restriction map for
pFN21ASDA0871
(Circular) (Six-base) MAPSORT of: pFN21ASDA0871.seq Check: 4720 from: 1 to: 6136
pFN21ASDA0871 6136 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3946 278
Size: 53 83 186 936 2410 2468
Fragments arranged by size:
2468 2410 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3479 1937
Size: 1542 4594
Acc65I G'GTAC_C
Cuts at: 3457 3457
Size: 6136
AclI AA'CG_TT
Cuts at: 1563 4263 4636 1563
Size: 2700 373 3063
Fragments arranged by size:
3063 2700 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2796 2849 4199 5583 5851 1632
Size: 1164 53 1350 1384 268 1917
Fragments arranged by size:
1917 1384 1350 1164 268 53
AflII C'TTAA_G
Cuts at: 820 1017 2489 820
Size: 197 1472 4467
Fragments arranged by size:
4467 1472 197
AflIII A'CryG_T
Cuts at: 1203 5035 6012 1203
Size: 3832 977 1327
Fragments arranged by size:
3832 1327 977
AgeI A'CCGG_T
Cuts at: 4967 4967
Size: 6136
AhdI GACnn_n'nnGTC
Cuts at: 4865 4865
Size: 6136
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6136
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3261 1184
Size: 2077 4059
AlwNI CAG_nnn'CTG
Cuts at: 3214 3879 5451 3214
Size: 665 1572 3899
Fragments arranged by size:
3899 1572 665
ApaLI G'TGCA_C
Cuts at: 4193 5349 4193
Size: 1156 4980
ApoI r'AATT_y
Cuts at: 1493 2111 2597 3445 3671 3823 5932 1493
Size: 618 486 848 226 152 2109 1697
Fragments arranged by size:
2109 1697 848 618 486 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2534 4690 160
Size: 872 1502 2156 1606
Fragments arranged by size:
2156 1606 1502 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6136
AvaI C'yCGr_G
Cuts at: 1870 1943 3461 1870
Size: 73 1518 4545
Fragments arranged by size:
4545 1518 73
BamHI G'GATC_C
Cuts at: 3466 3833 3466
Size: 367 5769
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3457 4912 618
Size: 325 217 627 1670 1455 1842
Fragments arranged by size:
1842 1670 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3232 3455 1391
Size: 1841 223 4072
Fragments arranged by size:
4072 1841 223
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2709 3323 928
Size: 971 810 614 3741
Fragments arranged by size:
3741 971 810 614
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2032 2066 2594 2628 4327 4361 1903
Size: 34 95 34 528 34 1699 34 3678
Fragments arranged by size:
3678 1699 528 95 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4028 5238 4028
Size: 1210 4926
BclI T'GATC_A
Cuts at: 1548 2571 1548
Size: 1023 5113
BfrBI ATG'CAT
Cuts at: 2882 2882
Size: 6136
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4747 136
Size: 107 122 71 631 605 3075 1525
Fragments arranged by size:
3075 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2476 6131 2476
Size: 3655 2481
BlpI GC'TnA_GC
Cuts at: 3553 3553
Size: 6136
Bme1580I G_kGCm'C
Cuts at: 1790 4197 5353 1790
Size: 2407 1156 2573
Fragments arranged by size:
2573 2407 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6136
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3867 4825 442
Size: 416 53 83 399 24 2450 958 1753
Fragments arranged by size:
2450 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6136
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2156 2156
Size: 6136
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2309 3131 3182 4796 1239
Size: 1070 822 51 1614 2579
Fragments arranged by size:
2579 1614 1070 822 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2732 3218 4261 5126 5424 2732
Size: 486 1043 865 298 3444
Fragments arranged by size:
3444 1043 865 486 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2087 4799 882
Size: 617 588 2712 2219
Fragments arranged by size:
2712 2219 617 588
BsaAI yAC'GTr
Cuts at: 493 1204 3433 3927 493
Size: 711 2229 494 2702
Fragments arranged by size:
2702 2229 711 494
BsaBI GATnn'nnATC
Cuts at: 3119 6130 3119
Size: 3011 3125
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2036 3943 4325 4960
Size: 53 83 186 936 503 1907 382 635
Cuts at: 4960 275
Size: 1451
Fragments arranged by size:
1907 1451 936 635 503 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4569 4967 5241 5388 5799 4569
Size: 398 274 147 411 4906
Fragments arranged by size:
4906 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5911
Fragments arranged by size:
5911 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2927 1186
Size: 1741 4395
BseYI C'CCAG_C
Cuts at: 5339 5835 5339
Size: 496 5640
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3420 1163
Size: 573 1684 3879
Fragments arranged by size:
3879 1684 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2046 3586 4347 5375 1540
Size: 275 186 45 1540 761 1028 2301
Fragments arranged by size:
2301 1540 1028 761 275 186 45
BsiHKAI G_wGCw'C
Cuts at: 2352 3232 4197 4282 5353 2352
Size: 880 965 85 1071 3135
Fragments arranged by size:
3135 1071 965 880 85
BsmI GAATG_Cn'
Cuts at: 2851 3647 3740 2851
Size: 796 93 5247
Fragments arranged by size:
5247 796 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2775 2775
Size: 6136
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2352 3232 3455 4197 4282 5353 1391
Size: 399 562 880 223 742 85 1071 2174
Fragments arranged by size:
2174 1071 880 742 562 399 223 85
BspEI T'CCGG_A
Cuts at: 5799 5799
Size: 6136
BspHI T'CATG_A
Cuts at: 2238 2619 4025 5755 2238
Size: 381 1406 1730 2619
Fragments arranged by size:
2619 1730 1406 381
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3491 844
Size: 2647 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4023 1394
Size: 429 2200 3507
Fragments arranged by size:
3507 2200 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4631 4805 65
Size: 1183 3383 174 1396
Fragments arranged by size:
3383 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4780 4967 1700
Size: 110 2970 187 2869
Fragments arranged by size:
2970 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6136
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6136
BssSI C'ACGA_G
Cuts at: 4196 5208 4196
Size: 1012 5124
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6136
BstBI TT'CG_AA
Cuts at: 2007 4940 2007
Size: 2933 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6136
BstYI r'GATC_y
Cuts at: 1972 2200 2476 3466 3833 4218 4235 5687 5773
Size: 228 276 990 367 385 17 1452 86
Cuts at: 5773 5785 6131 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 990 385 367 346 276 228
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2088 2124 513
Size: 552 1023 36 4525
Fragments arranged by size:
4525 1023 552 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5397
Fragments arranged by size:
5397 683 56
BtsI GCAGTG_nn'
Cuts at: 3652 4446 4466 3652
Size: 794 20 5322
Fragments arranged by size:
5322 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6136
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6101
DraI TTT'AAA
Cuts at: 3069 3440 3069
Size: 371 5765
DraIII CAC_nnn'GTG
Cuts at: 3050 3050
Size: 6136
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5143 809
Size: 507 3827 1802
Fragments arranged by size:
3827 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2247 3583 4472
Size: 54 1425 50 275 18 417 1336 889
Cuts at: 4472 6101 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1336 889 417 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3583 1537
Size: 275 1771 4090
Fragments arranged by size:
4090 1771 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2506 3069 3207 3306 4066 1353
Size: 1153 563 138 99 760 3423
Fragments arranged by size:
3423 1153 760 563 138 99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4723 5097 5243 387
Size: 1064 138 3134 374 146 1280
Fragments arranged by size:
3134 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3230 3230
Size: 6136
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2309 2796 2849 3131 3182 4199 4796
Size: 393 677 487 53 282 51 1017 597
Cuts at: 4796 5583 5851 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1017 787 677 597 487 393 282
268 53 51
EcoO109I rG'GnC_Cy
Cuts at: 2414 3011 2414
Size: 597 5539
EcoRI G'AATT_C
Cuts at: 2597 3445 3823 2597
Size: 848 378 4910
Fragments arranged by size:
4910 848 378
FspI TGC'GCA
Cuts at: 4642 4642
Size: 6136
HaeII r_GCGC'y
Cuts at: 1678 5283 1678
Size: 3605 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2150 2182 2696 2728 4777
Size: 32 135 32 578 32 514 32 2049
Cuts at: 4777 4809 4851 4883 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2049 578 514 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3480 3726 669
Size: 988 281 1542 246 3079
Fragments arranged by size:
3079 1542 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2435 2565 748
Size: 1687 130 4319
Fragments arranged by size:
4319 1687 130
HpaI GTT'AAC
Cuts at: 3726 3726
Size: 6136
KpnI G_GTAC'C
Cuts at: 3461 3461
Size: 6136
MfeI C'AATT_G
Cuts at: 3735 3735
Size: 6136
MluI A'CGCG_T
Cuts at: 6012 6012
Size: 6136
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2066 2701 2919 5250 5434 1305
Size: 761 635 218 2331 184 2007
Fragments arranged by size:
2331 2007 761 635 218 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2249 10
Size: 54 1425 343 417 3897
Fragments arranged by size:
3897 1425 417 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2243 2844 4094 4453 4612 518
Size: 648 1077 601 1250 359 159 2042
Fragments arranged by size:
2042 1250 1077 648 601 359 159
MspA1I CmG'CkG
Cuts at: 1606 3214 3552 4229 5377 5622 1606
Size: 1608 338 677 1148 245 2120
Fragments arranged by size:
2120 1608 1148 677 338 245
NcoI C'CATG_G
Cuts at: 513 1065 2088 2124 513
Size: 552 1023 36 4525
Fragments arranged by size:
4525 1023 552 36
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6136
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6136
NotI GC'GGCC_GC
Cuts at: 3583 3583
Size: 6136
NsiI A_TGCA'T
Cuts at: 2884 2884
Size: 6136
NspI r_CATG'y
Cuts at: 1132 1229 3494 5039 1132
Size: 97 2265 1545 2229
Fragments arranged by size:
2265 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6136
PciI A'CATG_T
Cuts at: 5035 5035
Size: 6136
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6136
PfoI T'CCnGG_A
Cuts at: 2482 2482
Size: 6136
PmeI GTTT'AAAC
Cuts at: 3440 3440
Size: 6136
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4205 4237 5718 5750 4205
Size: 32 1481 32 4591
Fragments arranged by size:
4591 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3011 3011
Size: 6136
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6136
PsiI TTA'TAA
Cuts at: 3706 3706
Size: 6136
PstI C_TGCA'G
Cuts at: 830 3034 3261 3488 830
Size: 2204 227 227 3478
Fragments arranged by size:
3478 2204 227 227
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6136
PvuII CAG'CTG
Cuts at: 3214 3214
Size: 6136
SacI G_AGCT'C
Cuts at: 3232 3232
Size: 6136
SalI G'TCGA_C
Cuts at: 1936 3478 1936
Size: 1542 4594
SanDI GG'GwC_CC
Cuts at: 3011 3011
Size: 6136
SapI GCTCTTCn'nnn_
Cuts at: 2506 2506
Size: 6136
SbfI CC_TGCA'GG
Cuts at: 3488 3488
Size: 6136
ScaI AGT'ACT
Cuts at: 4384 6096 4384
Size: 1712 4424
SfcI C'TryA_G
Cuts at: 826 1045 3030 3257 3484 4619 5300 5491 826
Size: 219 1985 227 227 1135 681 191 1471
Fragments arranged by size:
1985 1471 1135 681 227 227 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6136
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6136
SmaI CCC'GGG
Cuts at: 1872 3463 1872
Size: 1591 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2489 2747 3233 4240 5141 5403
Size: 197 926 546 258 486 1007 901 262
Cuts at: 5403 820
Size: 1553
Fragments arranged by size:
1553 1007 926 901 546 486 262 258
197
SnaBI TAC'GTA
Cuts at: 493 3433 3927 493
Size: 2940 494 2702
Fragments arranged by size:
2940 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6136
SphI G_CATG'C
Cuts at: 1132 3494 1132
Size: 2362 3774
SspI AAT'ATT
Cuts at: 5 52 4060 5
Size: 47 4008 2081
Fragments arranged by size:
4008 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6136
StyI C'CwwG_G
Cuts at: 513 1065 2088 2124 3575 513
Size: 552 1023 36 1451 3074
Fragments arranged by size:
3074 1451 1023 552 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2060 4163 4180 4333 4518 4963 325
Size: 1255 480 2103 17 153 185 445 1498
Fragments arranged by size:
2103 1498 1255 480 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4382 6094 96
Size: 274 80 33 51 1443 2405 1712 138
Fragments arranged by size:
2405 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6096 6128 6096
Size: 32 6104
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6136
XbaI T'CTAG_A
Cuts at: 2419 3472 2419
Size: 1053 5083
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6136
XmaI C'CCGG_G
Cuts at: 1870 3461 1870
Size: 1591 4545
XmnI GAAnn'nnTTC
Cuts at: 4265 4265
Size: 6136
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3944 276
Size: 53 83 186 936 2410 2468
Fragments arranged by size:
2468 2410 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI
SfiI SfoI SrfI SwaI XcmI