Restriction map for
pFN21ASDA0865
(Circular) (Six-base) MAPSORT of: pFN21ASDA0865.seq Check: 7833 from: 1 to: 10303
pFN21ASDA0865 10303 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3575 5529 6533 3575
Size: 1954 1004 7345
Fragments arranged by size:
7345 1954 1004
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8113 278
Size: 53 83 186 936 6577 2468
Fragments arranged by size:
6577 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5356 7646 1937
Size: 3419 2290 4594
Fragments arranged by size:
4594 3419 2290
Acc65I G'GTAC_C
Cuts at: 7624 7624
Size: 10303
AclI AA'CG_TT
Cuts at: 1563 8430 8803 1563
Size: 6867 373 3063
Fragments arranged by size:
6867 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2186 2282 2591 3555 3683 3858 3967 5099
Size: 554 96 309 964 128 175 109 1132
Cuts at: 5099 5510 5619 7308 7556 8366 9750 10018 1632
Size: 411 109 1689 248 810 1384 268 1917
Fragments arranged by size:
1917 1689 1384 1132 964 810 554 411
309 268 248 175 128 109 109 96
AfeI AGC'GCT
Cuts at: 3293 3293
Size: 10303
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10106
AflIII A'CryG_T
Cuts at: 1203 3613 5193 6444 9202 10179 1203
Size: 2410 1580 1251 2758 977 1327
Fragments arranged by size:
2758 2410 1580 1327 1251 977
AgeI A'CCGG_T
Cuts at: 3879 9134 3879
Size: 5255 5048
AhdI GACnn_n'nnGTC
Cuts at: 9032 9032
Size: 10303
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 10303
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 7583 1184
Size: 6399 3904
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3403 3435 3992 4024 5244 5276 3403
Size: 32 557 32 1220 32 8430
Fragments arranged by size:
8430 1220 557 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 4102 5521 6789 7477 8046 9618 4102
Size: 1419 1268 688 569 1572 4787
Fragments arranged by size:
4787 1572 1419 1268 688 569
ApaI G_GGCC'C
Cuts at: 6435 6906 6435
Size: 471 9832
ApaLI G'TGCA_C
Cuts at: 3873 5122 8360 9516 3873
Size: 1249 3238 1156 4660
Fragments arranged by size:
4660 3238 1249 1156
ApoI r'AATT_y
Cuts at: 1493 2352 3738 4291 4388 4615 4913 7215 7264
Size: 859 1386 553 97 227 298 2302 49
Cuts at: 7264 7612 7838 7990 10099 1493
Size: 348 226 152 2109 1697
Fragments arranged by size:
2302 2109 1697 1386 859 553 348 298
227 226 152 97 49
AseI AT'TA_AT
Cuts at: 160 1032 8857 160
Size: 872 7825 1606
Fragments arranged by size:
7825 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10303
AvaI C'yCGr_G
Cuts at: 1870 1943 2296 3197 5646 6134 6160 6188 6302
Size: 73 353 901 2449 488 26 28 114
Cuts at: 6302 6355 6761 7063 7628 1870
Size: 53 406 302 565 4545
Fragments arranged by size:
4545 2449 901 565 488 406 353 302
114 73 53 28 26
BamHI G'GATC_C
Cuts at: 4883 7633 8000 4883
Size: 2750 367 7186
Fragments arranged by size:
7186 2750 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3578 6155 6220 6859 7498
Size: 325 217 627 1791 2577 65 639 639
Cuts at: 7498 7624 9079 618
Size: 126 1455 1842
Fragments arranged by size:
2577 1842 1791 1455 639 639 627 325
217 126 65
BanII G_rGCy'C
Cuts at: 1391 3009 3258 3967 6368 6435 6632 6819 6906
Size: 1618 249 709 2401 67 197 187 87
Cuts at: 6906 7047 7494 7581 7622 1391
Size: 141 447 87 41 4072
Fragments arranged by size:
4072 2401 1618 709 447 249 197 187
141 87 87 67 41
BbeI G_GCGC'C
Cuts at: 3582 6159 6224 6863 3582
Size: 2577 65 639 7022
Fragments arranged by size:
7022 2577 639 65
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2400 928
Size: 971 501 8831
Fragments arranged by size:
8831 971 501
BbvCI CC'TCA_GC
Cuts at: 7385 7385
Size: 10303
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8494 8528 1903
Size: 34 6557 34 3678
Fragments arranged by size:
6557 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5246 7292 8195 9405 5246
Size: 2046 903 1210 6144
Fragments arranged by size:
6144 2046 1210 903
BclI T'GATC_A
Cuts at: 1548 2779 4845 1548
Size: 1231 2066 7006
Fragments arranged by size:
7006 2066 1231
BfrBI ATG'CAT
Cuts at: 2851 3911 2851
Size: 1060 9243
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3587 5826 6164
Size: 107 122 71 631 605 1915 2239 338
Cuts at: 6164 6487 6663 6784 6896 6953 6968 8914 136
Size: 323 176 121 112 57 15 1946 1525
Fragments arranged by size:
2239 1946 1915 1525 631 605 338 323
176 122 121 112 107 71 57 15
BglII A'GATC_T
Cuts at: 3309 5238 10298 3309
Size: 1929 5060 3314
Fragments arranged by size:
5060 3314 1929
BlpI GC'TnA_GC
Cuts at: 3248 5202 6098 7488 7720 3248
Size: 1954 896 1390 232 5831
Fragments arranged by size:
5831 1954 1390 896 232
Bme1580I G_kGCm'C
Cuts at: 1790 3877 5126 6276 6435 6906 8364 9520 1790
Size: 2087 1249 1150 159 471 1458 1156 2573
Fragments arranged by size:
2573 2087 1458 1249 1156 1150 471 159
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 10303
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 8034 8992 442
Size: 416 53 83 399 24 6617 958 1753
Fragments arranged by size:
6617 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10303
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4090 4090
Size: 10303
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2468 2487 2676 4079 5246 5351 6584 6608
Size: 1229 19 189 1403 1167 105 1233 24
Cuts at: 6608 6961 7000 7453 8963 1239
Size: 353 39 453 1510 2579
Fragments arranged by size:
2579 1510 1403 1233 1229 1167 453 353
189 105 39 24 19
Bpu10I CC'TnA_GC
Cuts at: 2992 7385 2992
Size: 4393 5910
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2982 6584 7173 8428 9293 9591 2982
Size: 3602 589 1255 865 298 3694
Fragments arranged by size:
3694 3602 1255 865 589 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2605 2754 4227 5847 6584 8966 882
Size: 617 1106 149 1473 1620 737 2382 2219
Fragments arranged by size:
2382 2219 1620 1473 1106 737 617 149
BsaAI yAC'GTr
Cuts at: 493 1204 5194 7600 8094 493
Size: 711 3990 2406 494 2702
Fragments arranged by size:
3990 2702 2406 711 494
BsaBI GATnn'nnATC
Cuts at: 10297 10297
Size: 10303
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2405 2894 3579 6156
Size: 53 83 186 936 872 489 685 2577
Cuts at: 6156 6221 6860 6960 8110 8492 9127 275
Size: 65 639 100 1150 382 635 1451
Fragments arranged by size:
2577 1451 1150 936 872 685 639 635
489 382 186 100 83 65 53
BsaWI w'CCGG_w
Cuts at: 3728 3879 8736 9134 9408 9555 9966 3728
Size: 151 4857 398 274 147 411 4065
Fragments arranged by size:
4857 4065 411 398 274 151 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2361 2391 2460 2490 2649
Size: 30 165 30 958 30 69 30 159
Cuts at: 2649 2679 2720 2750 2879 2909 3428 3458 3992
Size: 30 41 30 129 30 519 30 534
Cuts at: 3992 4022 4623 4653 5046 5076 5349 5379 5762
Size: 30 601 30 393 30 273 30 383
Cuts at: 5762 5792 6299 6314 6329 6344 1178
Size: 30 507 15 15 15 5137
Fragments arranged by size:
5137 958 601 534 519 507 393 383
273 165 159 129 69 41 30 30
30 30 30 30 30 30 30 30
30 30 30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2284 2382 2900 3394 3423 5067 6054 6128
Size: 1098 98 518 494 29 1644 987 74
Cuts at: 6128 6335 7084 7450 1186
Size: 207 749 366 4039
Fragments arranged by size:
4039 1644 1098 987 749 518 494 366
207 98 74 29
BseYI C'CCAG_C
Cuts at: 3387 5774 6268 7010 7526 9506 10002 3387
Size: 2387 494 742 516 1980 496 3688
Fragments arranged by size:
3688 2387 1980 742 516 496 494
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5837 6632 7463 1163
Size: 573 4101 795 831 4003
Fragments arranged by size:
4101 4003 831 795 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5799 6491 6718 7753 8514 9542
Size: 275 186 3798 692 227 1035 761 1028
Cuts at: 9542 1540
Size: 2301
Fragments arranged by size:
3798 2301 1035 1028 761 692 275 227
186
BsiHKAI G_wGCw'C
Cuts at: 2326 3258 3877 5126 7047 7581 8364 8449 9520
Size: 932 619 1249 1921 534 783 85 1071
Cuts at: 9520 2326
Size: 3109
Fragments arranged by size:
3109 1921 1249 1071 932 783 619 534
85
BsmI GAATG_Cn'
Cuts at: 5635 7814 7907 5635
Size: 2179 93 8031
Fragments arranged by size:
8031 2179 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2296 5898 2296
Size: 3602 6701
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2326 3009 3258 3877 3967 5126 6276
Size: 399 536 683 249 619 90 1159 1150
Cuts at: 6276 6368 6435 6632 6819 6906 7047 7494 7581
Size: 92 67 197 187 87 141 447 87
Cuts at: 7581 7622 8364 8449 9520 1391
Size: 41 742 85 1071 2174
Fragments arranged by size:
2174 1159 1150 1071 742 683 619 536
447 399 249 197 187 141 92 90
87 87 85 67 41
BspEI T'CCGG_A
Cuts at: 9966 9966
Size: 10303
BspHI T'CATG_A
Cuts at: 8192 9922 8192
Size: 1730 8573
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3193 3575 5489 5529 6483 6533 7658 844
Size: 2349 382 1914 40 954 50 1125 3489
Fragments arranged by size:
3489 2349 1914 1125 954 382 50 40
BsrBI CCG'CTC
Cuts at: 1394 1823 3183 8190 1394
Size: 429 1360 5007 3507
Fragments arranged by size:
5007 3507 1360 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2343 2415 5140 5640 8798 8972 65
Size: 1183 1095 72 2725 500 3158 174 1396
Fragments arranged by size:
3158 2725 1396 1183 1095 500 174 72
BsrFI r'CCGG_y
Cuts at: 1700 1810 3879 5658 6486 6971 8947 9134 1700
Size: 110 2069 1779 828 485 1976 187 2869
Fragments arranged by size:
2869 2069 1976 1779 828 485 187 110
BsrGI T'GTAC_A
Cuts at: 96 4254 4477 6255 96
Size: 4158 223 1778 4144
Fragments arranged by size:
4158 4144 1778 223
BssHII G'CGCG_C
Cuts at: 1925 6173 6204 6408 6792 1925
Size: 4248 31 204 384 5436
Fragments arranged by size:
5436 4248 384 204 31
BssSI C'ACGA_G
Cuts at: 2870 4460 8363 9375 2870
Size: 1590 3903 1012 3798
Fragments arranged by size:
3903 3798 1590 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4511 7477 794
Size: 3717 2966 3620
Fragments arranged by size:
3717 3620 2966
BstBI TT'CG_AA
Cuts at: 2007 9107 2007
Size: 7100 3203
BstEII G'GTnAC_C
Cuts at: 1184 4517 1184
Size: 3333 6970
BstYI r'GATC_y
Cuts at: 1972 3309 4883 5056 5238 6924 7473 7633 8000
Size: 1337 1574 173 182 1686 549 160 367
Cuts at: 8000 8385 8402 9854 9940 9952 10298 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1686 1574 1452 1337 549 385 367
346 182 173 160 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3406 3406
Size: 10303
BtgI C'CryG_G
Cuts at: 513 1065 2022 3378 4494 4714 5799 5897 5982
Size: 552 957 1356 1116 220 1085 98 85
Cuts at: 5982 6150 6379 6894 7089 7210 513
Size: 168 229 515 195 121 3606
Fragments arranged by size:
3606 1356 1116 1085 957 552 515 229
220 195 168 121 98 85
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5365 6372 487
Size: 683 56 4139 1007 4418
Fragments arranged by size:
4418 4139 1007 683 56
BtsI GCAGTG_nn'
Cuts at: 2588 5378 6206 7819 8613 8633 2588
Size: 2790 828 1613 794 20 4258
Fragments arranged by size:
4258 2790 1613 828 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10303
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10268
DraI TTT'AAA
Cuts at: 4180 4912 7607 4180
Size: 732 2695 6876
Fragments arranged by size:
6876 2695 732
DraIII CAC_nnn'GTG
Cuts at: 2872 5860 2872
Size: 2988 7315
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2369 9310 809
Size: 507 1053 6941 1802
Fragments arranged by size:
6941 1802 1053 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2062 2818 3787
Size: 54 1425 50 275 18 232 756 969
Cuts at: 3787 4242 5796 5985 5997 6279 6488 6715 6897
Size: 455 1554 189 12 282 209 227 182
Cuts at: 6897 7750 8639 10268 8
Size: 853 889 1629 43
Fragments arranged by size:
1629 1554 1425 969 889 853 756 455
282 275 232 227 209 189 182 54
50 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 5796 6488 6715 7750 1537
Size: 275 3984 692 227 1035 4090
Fragments arranged by size:
4090 3984 1035 692 275 227
EarI CTCTTCn'nnn_
Cuts at: 1353 2997 3009 3446 3479 4365 7061 8233 1353
Size: 1644 12 437 33 886 2696 1172 3423
Fragments arranged by size:
3423 2696 1644 1172 886 437 33 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4066 6971 8890 9264 9410 387
Size: 1064 138 2477 2905 1919 374 146 1280
Fragments arranged by size:
2905 2477 1919 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3256 7045 7579 3256
Size: 3789 534 5980
Fragments arranged by size:
5980 3789 534
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2186 2282 2468 2487 2591 2676 3555
Size: 393 554 96 186 19 104 85 879
Cuts at: 3555 3683 3858 3967 4079 5099 5246 5351 5510
Size: 128 175 109 112 1020 147 105 159
Cuts at: 5510 5619 6584 6608 6961 7000 7308 7453 7556
Size: 109 965 24 353 39 308 145 103
Cuts at: 7556 8366 8963 9750 10018 1239
Size: 810 597 787 268 1524
Fragments arranged by size:
1524 1020 965 879 810 787 597 554
393 353 308 268 186 175 159 147
145 128 112 109 109 105 104 103
96 85 39 24 19
EcoNI CCTnn'n_nnAGG
Cuts at: 2264 2483 4064 7561 2264
Size: 219 1581 3497 5006
Fragments arranged by size:
5006 3497 1581 219
EcoO109I rG'GnC_Cy
Cuts at: 2304 5221 5929 6126 6431 6432 6786 6903 7551
Size: 2917 708 197 305 1 354 117 648
Cuts at: 7551 2304
Size: 5056
Fragments arranged by size:
5056 2917 708 648 354 305 197 117
1
EcoRI G'AATT_C
Cuts at: 4291 7215 7612 7990 4291
Size: 2924 397 378 6604
Fragments arranged by size:
6604 2924 397 378
EcoRV GAT'ATC
Cuts at: 6120 6120
Size: 10303
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2267 4814 2267
Size: 2547 7756
FspI TGC'GCA
Cuts at: 8809 8809
Size: 10303
HaeII r_GCGC'y
Cuts at: 1678 3295 3582 5973 6159 6224 6701 6863 9450
Size: 1617 287 2391 186 65 477 162 2587
Cuts at: 9450 1678
Size: 2531
Fragments arranged by size:
2587 2531 2391 1617 477 287 186 162
65
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2361 2393 2460 2492 2570
Size: 32 135 32 789 32 67 32 78
Cuts at: 2570 2602 2649 2681 2720 2752 2879 2911 2963
Size: 32 47 32 39 32 127 32 52
Cuts at: 2963 2995 3815 3847 3896 3928 5046 5078 5270
Size: 32 820 32 49 32 1118 32 192
Cuts at: 5270 5302 5349 5381 6049 6081 6104 6136 7286
Size: 32 47 32 668 32 23 32 1150
Cuts at: 7286 7318 8944 8976 9018 9050 1373
Size: 32 1626 32 42 32 2626
Fragments arranged by size:
2626 1626 1150 1118 820 789 668 192
135 127 78 67 52 49 47 47
42 39 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 23
HincII GTy'rAC
Cuts at: 669 1657 1938 2374 2380 2553 2628 3346 4408
Size: 988 281 436 6 173 75 718 1062
Cuts at: 4408 5357 7647 7893 669
Size: 949 2290 246 3079
Fragments arranged by size:
3079 2290 1062 988 949 718 436 281
246 173 75 6
HindIII A'AGCT_T
Cuts at: 748 2603 748
Size: 1855 8448
HpaI GTT'AAC
Cuts at: 2628 3346 7893 2628
Size: 718 4547 5038
Fragments arranged by size:
5038 4547 718
KasI G'GCGC_C
Cuts at: 3578 6155 6220 6859 3578
Size: 2577 65 639 7022
Fragments arranged by size:
7022 2577 639 65
KpnI G_GTAC'C
Cuts at: 7628 7628
Size: 10303
MfeI C'AATT_G
Cuts at: 3072 7902 3072
Size: 4830 5473
MluI A'CGCG_T
Cuts at: 6444 10179 6444
Size: 3735 6568
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3075 6564 9417 9601 1305
Size: 1770 3489 2853 184 2007
Fragments arranged by size:
3489 2853 2007 1770 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2064 2820 3789 4244 10
Size: 54 1425 343 232 756 969 455 6069
Fragments arranged by size:
6069 1425 969 756 455 343 232 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2207 2235 2319 2388 4733 6263 8261
Size: 648 1041 28 84 69 2345 1530 1998
Cuts at: 8261 8620 8779 518
Size: 359 159 2042
Fragments arranged by size:
2345 2042 1998 1530 1041 648 359 159
84 69 28
MspA1I CmG'CkG
Cuts at: 1606 3391 3487 5342 5984 6102 6152 6634 7091
Size: 1785 96 1855 642 118 50 482 457
Cuts at: 7091 7384 7719 8396 9544 9789 1606
Size: 293 335 677 1148 245 2120
Fragments arranged by size:
2120 1855 1785 1148 677 642 482 457
335 293 245 118 96 50
NaeI GCC'GGC
Cuts at: 6973 6973
Size: 10303
NarI GG'CG_CC
Cuts at: 3579 6156 6221 6860 3579
Size: 2577 65 639 7022
Fragments arranged by size:
7022 2577 639 65
NcoI C'CATG_G
Cuts at: 513 1065 2022 3378 4494 7210 513
Size: 552 957 1356 1116 2716 3606
Fragments arranged by size:
3606 2716 1356 1116 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10303
NgoMIV G'CCGG_C
Cuts at: 6971 6971
Size: 10303
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10303
NotI GC'GGCC_GC
Cuts at: 7750 7750
Size: 10303
NsiI A_TGCA'T
Cuts at: 2853 3913 2853
Size: 1060 9243
NspI r_CATG'y
Cuts at: 1132 1229 2092 2174 2212 3617 3647 4330 5432
Size: 97 863 82 38 1405 30 683 1102
Cuts at: 5432 5719 7661 9206 1132
Size: 287 1942 1545 2229
Fragments arranged by size:
2229 1942 1545 1405 1102 863 683 287
97 82 38 30
PasI CC'CwG_GG
Cuts at: 1348 6437 7494 1348
Size: 5089 1057 4157
Fragments arranged by size:
5089 4157 1057
PciI A'CATG_T
Cuts at: 3613 9202 3613
Size: 5589 4714
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4102 1108
Size: 2994 7309
PfoI T'CCnGG_A
Cuts at: 2484 2745 3477 4101 5223 6585 2484
Size: 261 732 624 1122 1362 6202
Fragments arranged by size:
6202 1362 1122 732 624 261
PmeI GTTT'AAAC
Cuts at: 7607 7607
Size: 10303
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3992 4024 8372 8404 9885 9917 3992
Size: 32 4348 32 1481 32 4378
Fragments arranged by size:
4378 4348 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2304 5221 5929 6126 2304
Size: 2917 708 197 6481
Fragments arranged by size:
6481 2917 708 197
PshAI GACnn'nnGTC
Cuts at: 1613 4927 6518 1613
Size: 3314 1591 5398
Fragments arranged by size:
5398 3314 1591
PsiI TTA'TAA
Cuts at: 5477 7873 5477
Size: 2396 7907
PspOMI G'GGCC_C
Cuts at: 6431 6902 6431
Size: 471 9832
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5669 5701 5669
Size: 32 10271
PstI C_TGCA'G
Cuts at: 830 3572 5580 6911 7419 7655 830
Size: 2742 2008 1331 508 236 3478
Fragments arranged by size:
3478 2742 2008 1331 508 236
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10303
PvuII CAG'CTG
Cuts at: 3391 5342 7384 3391
Size: 1951 2042 6310
Fragments arranged by size:
6310 2042 1951
SacI G_AGCT'C
Cuts at: 3258 7047 7581 3258
Size: 3789 534 5980
Fragments arranged by size:
5980 3789 534
SacII CC_GC'GG
Cuts at: 5985 6153 7092 5985
Size: 168 939 9196
Fragments arranged by size:
9196 939 168
SalI G'TCGA_C
Cuts at: 1936 5355 7645 1936
Size: 3419 2290 4594
Fragments arranged by size:
4594 3419 2290
SanDI GG'GwC_CC
Cuts at: 2304 5929 6126 2304
Size: 3625 197 6481
Fragments arranged by size:
6481 3625 197
SapI GCTCTTCn'nnn_
Cuts at: 2997 3479 2997
Size: 482 9821
SbfI CC_TGCA'GG
Cuts at: 3572 7419 7655 3572
Size: 3847 236 6220
Fragments arranged by size:
6220 3847 236
ScaI AGT'ACT
Cuts at: 2786 6647 8551 10263 2786
Size: 3861 1904 1712 2826
Fragments arranged by size:
3861 2826 1904 1712
SfcI C'TryA_G
Cuts at: 826 1045 2213 2520 3568 5456 5576 6040 6781
Size: 219 1168 307 1048 1888 120 464 741
Cuts at: 6781 6907 7000 7415 7651 8786 9467 9658 826
Size: 126 93 415 236 1135 681 191 1471
Fragments arranged by size:
1888 1471 1168 1135 1048 741 681 464
415 307 236 219 191 126 120 93
SfoI GGC'GCC
Cuts at: 3580 6157 6222 6861 3580
Size: 2577 65 639 7022
Fragments arranged by size:
7022 2577 639 65
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10303
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10303
SmaI CCC'GGG
Cuts at: 1872 5648 6136 6162 6190 6304 6357 6763 7630
Size: 3776 488 26 28 114 53 406 867
Cuts at: 7630 1872
Size: 4545
Fragments arranged by size:
4545 3776 867 488 406 114 53 28
26
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2997 6599 7188 8407 9308 9570
Size: 197 926 1054 3602 589 1219 901 262
Cuts at: 9570 820
Size: 1553
Fragments arranged by size:
3602 1553 1219 1054 926 901 589 262
197
SnaBI TAC'GTA
Cuts at: 493 7600 8094 493
Size: 7107 494 2702
Fragments arranged by size:
7107 2702 494
SpeI A'CTAG_T
Cuts at: 152 4829 152
Size: 4677 5626
SphI G_CATG'C
Cuts at: 1132 2092 2174 4330 5432 7661 1132
Size: 960 82 2156 1102 2229 3774
Fragments arranged by size:
3774 2229 2156 1102 960 82
SrfI GCCC'GGGC
Cuts at: 6357 6763 6357
Size: 406 9897
SspI AAT'ATT
Cuts at: 5 52 4029 4175 4350 8227 5
Size: 47 3977 146 175 3877 2081
Fragments arranged by size:
3977 3877 2081 175 146 47
StuI AGG'CCT
Cuts at: 1517 7021 1517
Size: 5504 4799
StyI C'CwwG_G
Cuts at: 513 1065 2022 2059 3378 4036 4494 6864 7210
Size: 552 957 37 1319 658 458 2370 346
Cuts at: 7210 7742 513
Size: 532 3074
Fragments arranged by size:
3074 2370 1319 957 658 552 532 458
346 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 6888 8330 8347 8500 8685 9130 325
Size: 1255 5308 1442 17 153 185 445 1498
Fragments arranged by size:
5308 1498 1442 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2784 4254 4477
Size: 274 80 33 51 1443 807 1470 223
Cuts at: 4477 4668 6255 6645 8549 10261 96
Size: 191 1587 390 1904 1712 138
Fragments arranged by size:
1904 1712 1587 1470 1443 807 390 274
223 191 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3428 3460 5762 5794 6314 6346 10263 10295 3428
Size: 32 2302 32 520 32 3917 32 3436
Fragments arranged by size:
3917 3436 2302 520 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 10303
XbaI T'CTAG_A
Cuts at: 4990 7639 4990
Size: 2649 7654
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2492 4556 5822 6007 2492
Size: 2064 1266 185 6788
Fragments arranged by size:
6788 2064 1266 185
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10303
XmaI C'CCGG_G
Cuts at: 1870 5646 6134 6160 6188 6302 6355 6761 7628
Size: 3776 488 26 28 114 53 406 867
Cuts at: 7628 1870
Size: 4545
Fragments arranged by size:
4545 3776 867 488 406 114 53 28
26
XmnI GAAnn'nnTTC
Cuts at: 2250 4007 4374 5240 8432 2250
Size: 1757 367 866 3192 4121
Fragments arranged by size:
4121 3192 1757 866 367
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8111 276
Size: 53 83 186 936 6577 2468
Fragments arranged by size:
6577 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BsiWI BstXI BstZ17I FseI FspAI
NruI PacI PmlI PspXI RsrII SexAI SfiI SwaI