Restriction map for
pFN21ASDA0863
(Circular) (Six-base) MAPSORT of: pFN21ASDA0863.seq Check: 1664 from: 1 to: 8122
pFN21ASDA0863 8122 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4658 4658
Size: 8122
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5932 278
Size: 53 83 186 936 4396 2468
Fragments arranged by size:
4396 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4143 4539 4785 5465 1937
Size: 2206 396 246 680 4594
Fragments arranged by size:
4594 2206 680 396 246
Acc65I G'GTAC_C
Cuts at: 5443 5443
Size: 8122
AclI AA'CG_TT
Cuts at: 1563 6249 6622 1563
Size: 4686 373 3063
Fragments arranged by size:
4686 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2147 2377 4766 5114 6185 7569 7837 1632
Size: 515 230 2389 348 1071 1384 268 1917
Fragments arranged by size:
2389 1917 1384 1071 515 348 268 230
AfeI AGC'GCT
Cuts at: 2875 2875
Size: 8122
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7925
AflIII A'CryG_T
Cuts at: 1203 2489 7021 7998 1203
Size: 1286 4532 977 1327
Fragments arranged by size:
4532 1327 1286 977
AgeI A'CCGG_T
Cuts at: 6953 6953
Size: 8122
AhdI GACnn_n'nnGTC
Cuts at: 5111 6851 5111
Size: 1740 6382
AleI CACnn'nnGTG
Cuts at: 1166 4776 4896 1166
Size: 3610 120 4392
Fragments arranged by size:
4392 3610 120
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8122
AlwNI CAG_nnn'CTG
Cuts at: 2951 3952 5865 7437 2951
Size: 1001 1913 1572 3636
Fragments arranged by size:
3636 1913 1572 1001
ApaLI G'TGCA_C
Cuts at: 4227 6179 7335 4227
Size: 1952 1156 5014
Fragments arranged by size:
5014 1952 1156
ApoI r'AATT_y
Cuts at: 1493 2031 3637 5431 5657 5809 7918 1493
Size: 538 1606 1794 226 152 2109 1697
Fragments arranged by size:
2109 1794 1697 1606 538 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6676 160
Size: 872 5644 1606
Fragments arranged by size:
5644 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8122
AvaI C'yCGr_G
Cuts at: 1870 1943 4622 5038 5447 1870
Size: 73 2679 416 409 4545
Fragments arranged by size:
4545 2679 416 409 73
BamHI G'GATC_C
Cuts at: 5452 5819 5452
Size: 367 7755
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2963 3737 4398 4579 4616
Size: 325 217 627 1176 774 661 181 37
Cuts at: 4616 4713 5443 6898 618
Size: 97 730 1455 1842
Fragments arranged by size:
1842 1455 1176 774 730 661 627 325
217 181 97 37
BanII G_rGCy'C
Cuts at: 1391 3290 4128 5441 1391
Size: 1899 838 1313 4072
Fragments arranged by size:
4072 1899 1313 838
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4109 4992 928
Size: 971 2210 883 4058
Fragments arranged by size:
4058 2210 971 883
BbvCI CC'TCA_GC
Cuts at: 3078 3607 4611 3078
Size: 529 1004 6589
Fragments arranged by size:
6589 1004 529
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5047 5081 6313 6347 1903
Size: 34 3110 34 1232 34 3678
Fragments arranged by size:
3678 3110 1232 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4554 6014 7224 4554
Size: 1460 1210 5452
Fragments arranged by size:
5452 1460 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8122
BfrBI ATG'CAT
Cuts at: 4324 4930 4324
Size: 606 7516
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3703 6733 136
Size: 107 122 71 631 605 2031 3030 1525
Fragments arranged by size:
3030 2031 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2765 8117 2765
Size: 5352 2770
BlpI GC'TnA_GC
Cuts at: 5539 5539
Size: 8122
Bme1580I G_kGCm'C
Cuts at: 1790 2968 3740 4231 4619 6183 7339 1790
Size: 1178 772 491 388 1564 1156 2573
Fragments arranged by size:
2573 1564 1178 1156 772 491 388
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8122
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5853 6811 442
Size: 416 53 83 399 24 4436 958 1753
Fragments arranged by size:
4436 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8122
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2862 3010 3381 3927 3993 6782 1239
Size: 1623 148 371 546 66 2789 2579
Fragments arranged by size:
2789 2579 1623 546 371 148 66
Bpu10I CC'TnA_GC
Cuts at: 3078 3607 4611 3078
Size: 529 1004 6589
Fragments arranged by size:
6589 1004 529
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3163 4223 6247 7112 7410 3163
Size: 1060 2024 865 298 3875
Fragments arranged by size:
3875 2024 1060 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3028 3763 4294 6785 882
Size: 617 1529 735 531 2491 2219
Fragments arranged by size:
2491 2219 1529 735 617 531
BsaAI yAC'GTr
Cuts at: 493 1204 5419 5913 493
Size: 711 4215 494 2702
Fragments arranged by size:
4215 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 3941 8116 3941
Size: 4175 3947
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5929 6311 6946 275
Size: 53 83 186 936 4396 382 635 1451
Fragments arranged by size:
4396 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2415 6555 6953 7227 7374 7785 2415
Size: 4140 398 274 147 411 2752
Fragments arranged by size:
4140 2752 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3910 3940 1178
Size: 30 165 30 2507 30 5360
Fragments arranged by size:
5360 2507 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3592 3595 3996 4042 4045 5018 1186
Size: 2406 3 401 46 3 973 4290
Fragments arranged by size:
4290 2406 973 401 46 3 3
BseYI C'CCAG_C
Cuts at: 2929 3070 3234 4556 4875 7325 7821 2929
Size: 141 164 1322 319 2450 496 3230
Fragments arranged by size:
3230 2450 1322 496 319 164 141
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2832 3954 3984 1163
Size: 573 1096 1122 30 5301
Fragments arranged by size:
5301 1122 1096 573 30
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5572 6333 7361 1540
Size: 275 186 3571 761 1028 2301
Fragments arranged by size:
3571 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2869 4083 4128 4231 4386 4631 4846 6183 6268
Size: 1214 45 103 155 245 215 1337 85
Cuts at: 6268 7339 2869
Size: 1071 3652
Fragments arranged by size:
3652 1337 1214 1071 245 215 155 103
85 45
BsmI GAATG_Cn'
Cuts at: 4027 5633 5726 4027
Size: 1606 93 6423
Fragments arranged by size:
6423 1606 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2869 2968 3290 3740 4083 4128 4231
Size: 399 1079 99 322 450 343 45 103
Cuts at: 4231 4386 4619 4631 4846 5441 6183 6268 7339
Size: 155 233 12 215 595 742 85 1071
Cuts at: 7339 1391
Size: 2174
Fragments arranged by size:
2174 1079 1071 742 595 450 399 343
322 233 215 155 103 99 85 45
12
BspEI T'CCGG_A
Cuts at: 2415 7785 2415
Size: 5370 2752
BspHI T'CATG_A
Cuts at: 6011 7741 6011
Size: 1730 6392
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4115 4482 4658 4875 5477 844
Size: 3271 367 176 217 602 3489
Fragments arranged by size:
3489 3271 602 367 217 176
BsrBI CCG'CTC
Cuts at: 1394 1823 4531 6009 1394
Size: 429 2708 1478 3507
Fragments arranged by size:
3507 2708 1478 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2817 6617 6791 65
Size: 1183 1569 3800 174 1396
Fragments arranged by size:
3800 1569 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3895 3980 4631 6766 6953 1700
Size: 110 2085 85 651 2135 187 2869
Fragments arranged by size:
2869 2135 2085 651 187 110 85
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8122
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8122
BssSI C'ACGA_G
Cuts at: 6182 7194 6182
Size: 1012 7110
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4226 4773 794
Size: 3432 547 4143
Fragments arranged by size:
4143 3432 547
BstBI TT'CG_AA
Cuts at: 2007 6926 2007
Size: 4919 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8122
BstXI CCAn_nnnn'nTGG
Cuts at: 2380 4576 4778 4997 2380
Size: 2196 202 219 5505
Fragments arranged by size:
5505 2196 219 202
BstYI r'GATC_y
Cuts at: 1972 2646 2765 5146 5452 5819 6204 6221 7673
Size: 674 119 2381 306 367 385 17 1452
Cuts at: 7673 7759 7771 8117 1972
Size: 86 12 346 1977
Fragments arranged by size:
2381 1977 1452 674 385 367 346 306
119 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4605 4605
Size: 8122
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 7570
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2487 4279 4798 487
Size: 683 56 1261 1792 519 3811
Fragments arranged by size:
3811 1792 1261 683 519 56
BtsI GCAGTG_nn'
Cuts at: 2869 4103 4771 5638 6432 6452 2869
Size: 1234 668 867 794 20 4539
Fragments arranged by size:
4539 1234 867 794 668 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8122
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8087
DraI TTT'AAA
Cuts at: 5074 5426 5074
Size: 352 7770
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3502 7129 809
Size: 507 2186 3627 1802
Fragments arranged by size:
3627 2186 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3001 3457 3978
Size: 54 1425 50 275 18 1171 456 521
Cuts at: 3978 4969 5569 6458 8087 8
Size: 991 600 889 1629 43
Fragments arranged by size:
1629 1425 1171 991 889 600 521 456
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5569 1537
Size: 275 3757 4090
Fragments arranged by size:
4090 3757 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4293 5133 6052 1353
Size: 2940 840 919 3423
Fragments arranged by size:
3423 2940 919 840
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3918 6709 7083 7229 387
Size: 1064 138 2329 2791 374 146 1280
Fragments arranged by size:
2791 2329 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4126 4126
Size: 8122
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2147 2377 2862 3010 3381 3927 3993
Size: 393 515 230 485 148 371 546 66
Cuts at: 3993 4766 5114 6185 6782 7569 7837 1239
Size: 773 348 1071 597 787 268 1524
Fragments arranged by size:
1524 1071 787 773 597 546 515 485
393 371 348 268 230 148 66
EcoO109I rG'GnC_Cy
Cuts at: 2131 3245 3307 5105 2131
Size: 1114 62 1798 5148
Fragments arranged by size:
5148 1798 1114 62
EcoRI G'AATT_C
Cuts at: 3637 5431 5809 3637
Size: 1794 378 5950
Fragments arranged by size:
5950 1794 378
EcoRV GAT'ATC
Cuts at: 2225 4366 2225
Size: 2141 5981
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2105 2105
Size: 8122
FspI TGC'GCA
Cuts at: 6628 6628
Size: 8122
HaeII r_GCGC'y
Cuts at: 1678 2877 7269 1678
Size: 1199 4392 2531
Fragments arranged by size:
4392 2531 1199
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3813 3845 3910 3942 4172
Size: 32 135 32 2241 32 65 32 230
Cuts at: 4172 4204 4844 4876 5097 5129 6763 6795 6837
Size: 32 640 32 221 32 1634 32 42
Cuts at: 6837 6869 1373
Size: 32 2626
Fragments arranged by size:
2626 2241 1634 640 230 221 135 65
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5466 5712 669
Size: 988 281 3528 246 3079
Fragments arranged by size:
3528 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2759 4520 748
Size: 2011 1761 4350
Fragments arranged by size:
4350 2011 1761
HpaI GTT'AAC
Cuts at: 5712 5712
Size: 8122
KpnI G_GTAC'C
Cuts at: 5447 5447
Size: 8122
MfeI C'AATT_G
Cuts at: 5721 5721
Size: 8122
MluI A'CGCG_T
Cuts at: 7998 7998
Size: 8122
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3136 3157 3884 4161 7236 7420 1305
Size: 1831 21 727 277 3075 184 2007
Fragments arranged by size:
3075 2007 1831 727 277 184 21
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3003 3459 10
Size: 54 1425 343 1171 456 4673
Fragments arranged by size:
4673 1425 1171 456 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2313 4776 4896 6080 6439 6598 518
Size: 648 1147 2463 120 1184 359 159 2042
Fragments arranged by size:
2463 2042 1184 1147 648 359 159 120
MspA1I CmG'CkG
Cuts at: 1606 2112 3611 3968 4993 5538 6215 7363 7608
Size: 506 1499 357 1025 545 677 1148 245
Cuts at: 7608 1606
Size: 2120
Fragments arranged by size:
2120 1499 1148 1025 677 545 506 357
245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7570
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8122
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8122
NotI GC'GGCC_GC
Cuts at: 5569 5569
Size: 8122
NsiI A_TGCA'T
Cuts at: 4326 4932 4326
Size: 606 7516
NspI r_CATG'y
Cuts at: 1132 1229 2493 2543 4000 5480 7025 1132
Size: 97 1264 50 1457 1480 1545 2229
Fragments arranged by size:
2229 1545 1480 1457 1264 97 50
PacI TTA_AT'TAA
Cuts at: 2571 2571
Size: 8122
PasI CC'CwG_GG
Cuts at: 1348 2959 3215 3302 3422 1348
Size: 1611 256 87 120 6048
Fragments arranged by size:
6048 1611 256 120 87
PciI A'CATG_T
Cuts at: 2489 7021 2489
Size: 4532 3590
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2332 2858 3241 3733 4098 1108
Size: 1224 526 383 492 365 5132
Fragments arranged by size:
5132 1224 526 492 383 365
PfoI T'CCnGG_A
Cuts at: 2149 2149
Size: 8122
PmeI GTTT'AAAC
Cuts at: 5426 5426
Size: 8122
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6191 6223 7704 7736 6191
Size: 32 1481 32 6577
Fragments arranged by size:
6577 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2131 3245 3307 5105 2131
Size: 1114 62 1798 5148
Fragments arranged by size:
5148 1798 1114 62
PshAI GACnn'nnGTC
Cuts at: 1613 3020 1613
Size: 1407 6715
PsiI TTA'TAA
Cuts at: 5692 5692
Size: 8122
PstI C_TGCA'G
Cuts at: 830 3066 3315 3376 3394 3412 3556 3654 4063
Size: 2236 249 61 18 18 144 98 409
Cuts at: 4063 4838 4872 5474 830
Size: 775 34 602 3478
Fragments arranged by size:
3478 2236 775 602 409 249 144 98
61 34 18 18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8122
PvuII CAG'CTG
Cuts at: 2112 3611 3968 2112
Size: 1499 357 6266
Fragments arranged by size:
6266 1499 357
SacI G_AGCT'C
Cuts at: 4128 4128
Size: 8122
SalI G'TCGA_C
Cuts at: 1936 5464 1936
Size: 3528 4594
SanDI GG'GwC_CC
Cuts at: 3245 3245
Size: 8122
SapI GCTCTTCn'nnn_
Cuts at: 4293 5133 4293
Size: 840 7282
SbfI CC_TGCA'GG
Cuts at: 3315 3376 3394 3412 4872 5474 3315
Size: 61 18 18 1460 602 5963
Fragments arranged by size:
5963 1460 602 61 18 18
ScaI AGT'ACT
Cuts at: 6370 8082 6370
Size: 1712 6410
SexAI A'CCwGG_T
Cuts at: 3772 4074 4146 3772
Size: 302 72 7748
Fragments arranged by size:
7748 302 72
SfcI C'TryA_G
Cuts at: 826 1045 2524 2637 3062 3311 3372 3390 3408
Size: 219 1479 113 425 249 61 18 18
Cuts at: 3408 3552 3650 3834 4059 4834 4868 5470 6605
Size: 144 98 184 225 775 34 602 1135
Cuts at: 6605 7286 7477 826
Size: 681 191 1471
Fragments arranged by size:
1479 1471 1135 775 681 602 425 249
225 219 191 184 144 113 98 61
34 18 18
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8122
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8122
SmaI CCC'GGG
Cuts at: 1872 4624 5040 5449 1872
Size: 2752 416 409 4545
Fragments arranged by size:
4545 2752 416 409
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3142 4202 6226 7127 7389 820
Size: 197 926 1199 1060 2024 901 262 1553
Fragments arranged by size:
2024 1553 1199 1060 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5419 5913 493
Size: 4926 494 2702
Fragments arranged by size:
4926 2702 494
SpeI A'CTAG_T
Cuts at: 152 3182 152
Size: 3030 5092
SphI G_CATG'C
Cuts at: 1132 4000 5480 1132
Size: 2868 1480 3774
Fragments arranged by size:
3774 2868 1480
SspI AAT'ATT
Cuts at: 5 52 2443 2666 6046 5
Size: 47 2391 223 3380 2081
Fragments arranged by size:
3380 2391 2081 223 47
StuI AGG'CCT
Cuts at: 1517 3706 3912 4049 4603 4688 1517
Size: 2189 206 137 554 85 4951
Fragments arranged by size:
4951 2189 554 206 137 85
StyI C'CwwG_G
Cuts at: 513 1065 4848 5561 513
Size: 552 3783 713 3074
Fragments arranged by size:
3783 3074 713 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2594 3248 5254 6149 6166 6319 6504
Size: 1255 1014 654 2006 895 17 153 185
Cuts at: 6504 6949 325
Size: 445 1498
Fragments arranged by size:
2006 1498 1255 1014 895 654 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2520 5347 6368
Size: 274 80 33 51 1443 543 2827 1021
Cuts at: 6368 8080 96
Size: 1712 138
Fragments arranged by size:
2827 1712 1443 1021 543 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2202 2234 2931 2963 4166 4198 8082 8114 2202
Size: 32 697 32 1203 32 3884 32 2210
Fragments arranged by size:
3884 2210 1203 697 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8122
XbaI T'CTAG_A
Cuts at: 5458 5458
Size: 8122
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2966 4239 2966
Size: 1273 6849
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8122
XmaI C'CCGG_G
Cuts at: 1870 4622 5038 5447 1870
Size: 2752 416 409 4545
Fragments arranged by size:
4545 2752 416 409
XmnI GAAnn'nnTTC
Cuts at: 6251 6251
Size: 8122
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5930 276
Size: 53 83 186 936 4396 2468
Fragments arranged by size:
4396 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI
BsmBI BstZ17I DraIII EcoNI FseI FspAI KasI NaeI
NarI NgoMIV NruI PmlI PspOMI PspXI PsrI RsrII
SacII SfiI SfoI SrfI SwaI