Restriction map for
pFN21ASDA0862
(Circular) (Six-base) MAPSORT of: pFN21ASDA0862.seq Check: 1862 from: 1 to: 6475
pFN21ASDA0862 6475 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4285 278
Size: 53 83 186 936 2749 2468
Fragments arranged by size:
2749 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3768 3818 1937
Size: 1831 50 4594
Fragments arranged by size:
4594 1831 50
Acc65I G'GTAC_C
Cuts at: 3796 3796
Size: 6475
AclI AA'CG_TT
Cuts at: 1563 4602 4975 1563
Size: 3039 373 3063
Fragments arranged by size:
3063 3039 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2562 3573 4538 5922 6190 1632
Size: 930 1011 965 1384 268 1917
Fragments arranged by size:
1917 1384 1011 965 930 268
AflII C'TTAA_G
Cuts at: 820 1017 3431 820
Size: 197 2414 3864
Fragments arranged by size:
3864 2414 197
AflIII A'CryG_T
Cuts at: 1203 5374 6351 1203
Size: 4171 977 1327
Fragments arranged by size:
4171 1327 977
AgeI A'CCGG_T
Cuts at: 5306 5306
Size: 6475
AhdI GACnn_n'nnGTC
Cuts at: 5204 5204
Size: 6475
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6475
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6475
AlwNI CAG_nnn'CTG
Cuts at: 2258 4218 5790 2258
Size: 1960 1572 2943
Fragments arranged by size:
2943 1960 1572
ApaLI G'TGCA_C
Cuts at: 2943 4532 5688 2943
Size: 1589 1156 3730
Fragments arranged by size:
3730 1589 1156
ApoI r'AATT_y
Cuts at: 1493 2323 2488 2571 2959 3129 3141 3244 3784
Size: 830 165 83 388 170 12 103 540
Cuts at: 3784 4010 4162 6271 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 830 540 388 226 170 165
152 103 83 12
AseI AT'TA_AT
Cuts at: 160 1032 5029 160
Size: 872 3997 1606
Fragments arranged by size:
3997 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6475
AvaI C'yCGr_G
Cuts at: 1870 1943 3800 1870
Size: 73 1857 4545
Fragments arranged by size:
4545 1857 73
BamHI G'GATC_C
Cuts at: 3805 4172 3805
Size: 367 6108
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3796 5251 618
Size: 325 217 627 2009 1455 1842
Fragments arranged by size:
2009 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2304 3794 1391
Size: 913 1490 4072
Fragments arranged by size:
4072 1490 913
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5504
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4666 4700 1903
Size: 34 2729 34 3678
Fragments arranged by size:
3678 2729 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3069 4367 5577 3069
Size: 1298 1210 3967
Fragments arranged by size:
3967 1298 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6475
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5086 136
Size: 107 122 71 631 605 3414 1525
Fragments arranged by size:
3414 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2351 6470 2351
Size: 4119 2356
BlpI GC'TnA_GC
Cuts at: 2681 3892 2681
Size: 1211 5264
Bme1580I G_kGCm'C
Cuts at: 1790 2947 4536 5692 1790
Size: 1157 1589 1156 2573
Fragments arranged by size:
2573 1589 1157 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6475
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4206 5164 442
Size: 416 53 83 399 24 2789 958 1753
Fragments arranged by size:
2789 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6475
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2136 2176 3133 3590 5135 1239
Size: 897 40 957 457 1545 2579
Fragments arranged by size:
2579 1545 957 897 457 40
Bpu10I CC'TnA_GC
Cuts at: 3034 3034
Size: 6475
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3323 3647 4600 5465 5763 3323
Size: 324 953 865 298 4035
Fragments arranged by size:
4035 953 865 324 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3296 5138 882
Size: 617 1797 1842 2219
Fragments arranged by size:
2219 1842 1797 617
BsaAI yAC'GTr
Cuts at: 493 1204 3772 4266 493
Size: 711 2568 494 2702
Fragments arranged by size:
2702 2568 711 494
BsaBI GATnn'nnATC
Cuts at: 6469 6469
Size: 6475
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4282 4664 5299 275
Size: 53 83 186 936 2749 382 635 1451
Fragments arranged by size:
2749 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4908 5306 5580 5727 6138 4908
Size: 398 274 147 411 5245
Fragments arranged by size:
5245 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6250
Fragments arranged by size:
6250 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6475
BseYI C'CCAG_C
Cuts at: 2251 2437 3707 5678 6174 2251
Size: 186 1270 1971 496 2552
Fragments arranged by size:
2552 1971 1270 496 186
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5902
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3925 4686 5714 1540
Size: 275 186 1924 761 1028 2301
Fragments arranged by size:
2301 1924 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2304 2947 4536 4621 5692 2304
Size: 643 1589 85 1071 3087
Fragments arranged by size:
3087 1589 1071 643 85
BsmI GAATG_Cn'
Cuts at: 2685 3986 4079 2685
Size: 1301 93 5081
Fragments arranged by size:
5081 1301 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2304 2947 3794 4536 4621 5692 1391
Size: 399 514 643 847 742 85 1071 2174
Fragments arranged by size:
2174 1071 847 742 643 514 399 85
BspEI T'CCGG_A
Cuts at: 6138 6138
Size: 6475
BspHI T'CATG_A
Cuts at: 2466 3657 4364 6094 2466
Size: 1191 707 1730 2847
Fragments arranged by size:
2847 1730 1191 707
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2722 3618 3830 844
Size: 1878 896 212 3489
Fragments arranged by size:
3489 1878 896 212
BsrBI CCG'CTC
Cuts at: 1394 1823 4362 1394
Size: 429 2539 3507
Fragments arranged by size:
3507 2539 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2287 4970 5144 65
Size: 1183 1039 2683 174 1396
Fragments arranged by size:
2683 1396 1183 1039 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5119 5306 1700
Size: 110 3309 187 2869
Fragments arranged by size:
3309 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2801 96
Size: 2705 3770
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6475
BssSI C'ACGA_G
Cuts at: 4535 5547 4535
Size: 1012 5463
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6475
BstBI TT'CG_AA
Cuts at: 2007 5279 2007
Size: 3272 3203
BstEII G'GTnAC_C
Cuts at: 1184 3494 1184
Size: 2310 4165
BstXI CCAn_nnnn'nTGG
Cuts at: 3353 3353
Size: 6475
BstYI r'GATC_y
Cuts at: 1972 2065 2351 3270 3382 3805 4172 4557 4574
Size: 93 286 919 112 423 367 385 17
Cuts at: 4574 6026 6112 6124 6470 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 919 423 385 367 346 286
112 93 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3276 3362 3276
Size: 86 6389
BtgI C'CryG_G
Cuts at: 513 1065 3346 3762 513
Size: 552 2281 416 3226
Fragments arranged by size:
3226 2281 552 416
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5736
Fragments arranged by size:
5736 683 56
BtsI GCAGTG_nn'
Cuts at: 2947 3991 4785 4805 2947
Size: 1044 794 20 4617
Fragments arranged by size:
4617 1044 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6475
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6440
DraI TTT'AAA
Cuts at: 3779 3779
Size: 6475
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5482 809
Size: 507 4166 1802
Fragments arranged by size:
4166 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2243 3922 4811
Size: 54 1425 50 275 18 413 1679 889
Cuts at: 4811 6440 8
Size: 1629 43
Fragments arranged by size:
1679 1629 1425 889 413 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3922 1537
Size: 275 2110 4090
Fragments arranged by size:
4090 2110 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2313 3384 3483 4405 1353
Size: 960 1071 99 922 3423
Fragments arranged by size:
3423 1071 960 922 99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5062 5436 5582 387
Size: 1064 138 3473 374 146 1280
Fragments arranged by size:
3473 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2302 2302
Size: 6475
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2136 2176 2562 3133 3573 3590 4538
Size: 393 504 40 386 571 440 17 948
Cuts at: 4538 5135 5922 6190 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 948 787 597 571 504 440 393
386 268 40 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2783 2783
Size: 6475
EcoO109I rG'GnC_Cy
Cuts at: 3713 3713
Size: 6475
EcoRI G'AATT_C
Cuts at: 2323 3784 4162 2323
Size: 1461 378 4636
Fragments arranged by size:
4636 1461 378
FspI TGC'GCA
Cuts at: 4981 4981
Size: 6475
HaeII r_GCGC'y
Cuts at: 1678 5622 1678
Size: 3944 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2461 2493 3269 3301 3733
Size: 32 135 32 889 32 776 32 432
Cuts at: 3733 3765 5116 5148 5190 5222 1373
Size: 32 1351 32 42 32 2626
Fragments arranged by size:
2626 1351 889 776 432 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2227 2388 3195 3471 3819 4065
Size: 988 281 289 161 807 276 348 246
Cuts at: 4065 669
Size: 3079
Fragments arranged by size:
3079 988 807 348 289 281 276 246
161
HindIII A'AGCT_T
Cuts at: 748 3338 3647 748
Size: 2590 309 3576
Fragments arranged by size:
3576 2590 309
HpaI GTT'AAC
Cuts at: 3195 4065 3195
Size: 870 5605
KpnI G_GTAC'C
Cuts at: 3800 3800
Size: 6475
MfeI C'AATT_G
Cuts at: 4074 4074
Size: 6475
MluI A'CGCG_T
Cuts at: 6351 6351
Size: 6475
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3360 5589 5773 1305
Size: 2055 2229 184 2007
Fragments arranged by size:
2229 2055 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4653
Fragments arranged by size:
4653 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4433 4792 4951 518
Size: 648 3267 359 159 2042
Fragments arranged by size:
3267 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3891 4568 5716 5961 1606
Size: 2285 677 1148 245 2120
Fragments arranged by size:
2285 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3346 3762 513
Size: 552 2281 416 3226
Fragments arranged by size:
3226 2281 552 416
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6475
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6475
NotI GC'GGCC_GC
Cuts at: 3922 3922
Size: 6475
NspI r_CATG'y
Cuts at: 1132 1229 2680 3833 5378 1132
Size: 97 1451 1153 1545 2229
Fragments arranged by size:
2229 1545 1451 1153 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6475
PciI A'CATG_T
Cuts at: 5374 5374
Size: 6475
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2258 3068 1108
Size: 1150 810 4515
Fragments arranged by size:
4515 1150 810
PmeI GTTT'AAAC
Cuts at: 3779 3779
Size: 6475
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4544 4576 6057 6089 4544
Size: 32 1481 32 4930
Fragments arranged by size:
4930 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6475
PsiI TTA'TAA
Cuts at: 4045 4045
Size: 6475
PstI C_TGCA'G
Cuts at: 830 3644 3827 830
Size: 2814 183 3478
Fragments arranged by size:
3478 2814 183
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6475
SacI G_AGCT'C
Cuts at: 2304 2304
Size: 6475
SalI G'TCGA_C
Cuts at: 1936 3817 1936
Size: 1881 4594
SbfI CC_TGCA'GG
Cuts at: 3827 3827
Size: 6475
ScaI AGT'ACT
Cuts at: 4723 6435 4723
Size: 1712 4763
SfcI C'TryA_G
Cuts at: 826 1045 2859 3640 3823 4958 5639 5830 826
Size: 219 1814 781 183 1135 681 191 1471
Fragments arranged by size:
1814 1471 1135 781 681 219 191 183
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6475
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6475
SmaI CCC'GGG
Cuts at: 1872 3802 1872
Size: 1930 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3302 3431 3626 4579 5480 5742
Size: 197 926 1359 129 195 953 901 262
Cuts at: 5742 820
Size: 1553
Fragments arranged by size:
1553 1359 953 926 901 262 197 195
129
SnaBI TAC'GTA
Cuts at: 493 3772 4266 493
Size: 3279 494 2702
Fragments arranged by size:
3279 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6475
SphI G_CATG'C
Cuts at: 1132 2680 3833 1132
Size: 1548 1153 3774
Fragments arranged by size:
3774 1548 1153
SspI AAT'ATT
Cuts at: 5 52 4399 5
Size: 47 4347 2081
Fragments arranged by size:
4347 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6475
StyI C'CwwG_G
Cuts at: 513 1065 2085 3207 3346 3523 3762 3914 513
Size: 552 1020 1122 139 177 239 152 3074
Fragments arranged by size:
3074 1122 1020 552 239 177 152 139
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3056 4502 4519 4672 4857 5302 325
Size: 1255 1476 1446 17 153 185 445 1498
Fragments arranged by size:
1498 1476 1446 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2801 4721 6433
Size: 274 80 33 51 1443 824 1920 1712
Cuts at: 6433 96
Size: 138
Fragments arranged by size:
1920 1712 1443 824 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6435 6467 6435
Size: 32 6443
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6475
XbaI T'CTAG_A
Cuts at: 3385 3811 3385
Size: 426 6049
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6475
XmaI C'CCGG_G
Cuts at: 1870 3800 1870
Size: 1930 4545
XmnI GAAnn'nnTTC
Cuts at: 2778 4604 2778
Size: 1826 4649
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4283 276
Size: 53 83 186 936 2749 2468
Fragments arranged by size:
2749 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI