Restriction map for
pFN21ASDA0857
(Circular) (Six-base) MAPSORT of: pFN21ASDA0857.seq Check: 9962 from: 1 to: 6688
pFN21ASDA0857 6688 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4498 278
Size: 53 83 186 936 2962 2468
Fragments arranged by size:
2962 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2382 4031 1937
Size: 445 1649 4594
Fragments arranged by size:
4594 1649 445
Acc65I G'GTAC_C
Cuts at: 4009 4009
Size: 6688
AclI AA'CG_TT
Cuts at: 1563 4815 5188 1563
Size: 3252 373 3063
Fragments arranged by size:
3252 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2575 2904 2978 3797 4751 6135 6403 1632
Size: 943 329 74 819 954 1384 268 1917
Fragments arranged by size:
1917 1384 954 943 819 329 268 74
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6491
AflIII A'CryG_T
Cuts at: 1203 2151 5587 6564 1203
Size: 948 3436 977 1327
Fragments arranged by size:
3436 1327 977 948
AgeI A'CCGG_T
Cuts at: 5519 5519
Size: 6688
AhdI GACnn_n'nnGTC
Cuts at: 5417 5417
Size: 6688
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6688
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2756 1184
Size: 1572 5116
AlwNI CAG_nnn'CTG
Cuts at: 3898 4431 6003 3898
Size: 533 1572 4583
Fragments arranged by size:
4583 1572 533
ApaI G_GGCC'C
Cuts at: 2107 2266 2306 2983 3074 3301 3974 2107
Size: 159 40 677 91 227 673 4821
Fragments arranged by size:
4821 677 673 227 159 91 40
ApaLI G'TGCA_C
Cuts at: 4745 5901 4745
Size: 1156 5532
ApoI r'AATT_y
Cuts at: 1493 3997 4223 4375 6484 1493
Size: 2504 226 152 2109 1697
Fragments arranged by size:
2504 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5242 160
Size: 872 4210 1606
Fragments arranged by size:
4210 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6688
AvaI C'yCGr_G
Cuts at: 1870 1943 2128 2222 2976 3097 3967 4013 1870
Size: 73 185 94 754 121 870 46 4545
Fragments arranged by size:
4545 870 754 185 121 94 73 46
BamHI G'GATC_C
Cuts at: 2677 4018 4385 2677
Size: 1341 367 4980
Fragments arranged by size:
4980 1341 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2308 2834 3081 4009 5464
Size: 325 217 627 521 526 247 928 1455
Cuts at: 5464 618
Size: 1842
Fragments arranged by size:
1842 1455 928 627 526 521 325 247
217
BanII G_rGCy'C
Cuts at: 1391 2107 2129 2266 2306 2327 2783 2983 3066
Size: 716 22 137 40 21 456 200 83
Cuts at: 3066 3074 3104 3301 3382 3837 3974 4007 1391
Size: 8 30 197 81 455 137 33 4072
Fragments arranged by size:
4072 716 456 455 200 197 137 137
83 81 40 33 30 22 21 8
BbeI G_GCGC'C
Cuts at: 2312 2838 2312
Size: 526 6162
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2213 2416 928
Size: 971 314 203 5200
Fragments arranged by size:
5200 971 314 203
BbvCI CC'TCA_GC
Cuts at: 2800 2800
Size: 6688
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4879 4913 1903
Size: 34 2942 34 3678
Fragments arranged by size:
3678 2942 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4580 5790 4580
Size: 1210 5478
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6688
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2408 3269 3746
Size: 107 122 71 631 605 736 861 477
Cuts at: 3746 5299 136
Size: 1553 1525
Fragments arranged by size:
1553 1525 861 736 631 605 477 122
107 71
BglII A'GATC_T
Cuts at: 6683 6683
Size: 6688
BlpI GC'TnA_GC
Cuts at: 3869 4105 3869
Size: 236 6452
Bme1580I G_kGCm'C
Cuts at: 1790 2107 2266 2306 2983 3074 3301 3974 4749
Size: 317 159 40 677 91 227 673 775
Cuts at: 4749 5905 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 775 677 673 317 227 159
91 40
BmgBI CAC'GTC
Cuts at: 1318 2083 1318
Size: 765 5923
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2755 3582 4419
Size: 416 53 83 399 24 1338 827 837
Cuts at: 4419 5377 442
Size: 958 1753
Fragments arranged by size:
1753 1338 958 837 827 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6688
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2257 2257
Size: 6688
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3303 3643 3710 3920 5348 1239
Size: 2064 340 67 210 1428 2579
Fragments arranged by size:
2579 2064 1428 340 210 67
Bpu10I CC'TnA_GC
Cuts at: 2540 2800 3246 3677 2540
Size: 260 446 431 5551
Fragments arranged by size:
5551 446 431 260
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3577 4813 5678 5976 3577
Size: 1236 865 298 4289
Fragments arranged by size:
4289 1236 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2589 3416 3932 5351 882
Size: 617 1090 827 516 1419 2219
Fragments arranged by size:
2219 1419 1090 827 617 516
BsaAI yAC'GTr
Cuts at: 493 1204 3985 4479 493
Size: 711 2781 494 2702
Fragments arranged by size:
2781 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 6682 6682
Size: 6688
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2309 2379 2835 4495
Size: 53 83 186 936 776 70 456 1660
Cuts at: 4495 4877 5512 275
Size: 382 635 1451
Fragments arranged by size:
1660 1451 936 776 635 456 382 186
83 70 53
BsaWI w'CCGG_w
Cuts at: 5121 5519 5793 5940 6351 5121
Size: 398 274 147 411 5458
Fragments arranged by size:
5458 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3074 3104 1178
Size: 30 165 30 1671 30 4762
Fragments arranged by size:
4762 1671 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2249 2834 2961 2964 3074 3167 3248 3302
Size: 1063 585 127 3 110 93 81 54
Cuts at: 3302 3305 3340 1186
Size: 3 35 4534
Fragments arranged by size:
4534 1063 585 127 110 93 81 54
35 3 3
BseYI C'CCAG_C
Cuts at: 2442 2776 5891 6387 2442
Size: 334 3115 496 2743
Fragments arranged by size:
3115 2743 496 334
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2450 2886 3911 1163
Size: 573 714 436 1025 3940
Fragments arranged by size:
3940 1025 714 573 436
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3916 4138 4899 5927 1540
Size: 275 186 1915 222 761 1028 2301
Fragments arranged by size:
2301 1915 1028 761 275 222 186
BsiHKAI G_wGCw'C
Cuts at: 2129 2243 2327 2352 3247 3837 4749 4834 5905
Size: 114 84 25 895 590 912 85 1071
Cuts at: 5905 2129
Size: 2912
Fragments arranged by size:
2912 1071 912 895 590 114 85 84
25
BsmI GAATG_Cn'
Cuts at: 3720 4199 4292 3720
Size: 479 93 6116
Fragments arranged by size:
6116 479 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2107 2129 2243 2266 2306 2327 2352
Size: 399 317 22 114 23 40 21 25
Cuts at: 2352 2783 2983 3066 3074 3104 3247 3301 3382
Size: 431 200 83 8 30 143 54 81
Cuts at: 3382 3837 3974 4007 4749 4834 5905 1391
Size: 455 137 33 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 455 431 399 317 200
143 137 114 85 83 81 54 40
33 30 25 23 22 21 8
BspEI T'CCGG_A
Cuts at: 6351 6351
Size: 6688
BspHI T'CATG_A
Cuts at: 4577 6307 4577
Size: 1730 4958
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4043 844
Size: 3199 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2135 2367 2853 3021 3657 3862 4575
Size: 429 312 232 486 168 636 205 713
Cuts at: 4575 1394
Size: 3507
Fragments arranged by size:
3507 713 636 486 429 312 232 205
168
BsrDI GCAATG_nn'
Cuts at: 65 1248 5183 5357 65
Size: 1183 3935 174 1396
Fragments arranged by size:
3935 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2047 3915 5332 5519 1700
Size: 110 237 1868 1417 187 2869
Fragments arranged by size:
2869 1868 1417 237 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6688
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6688
BssSI C'ACGA_G
Cuts at: 4748 5760 4748
Size: 1012 5676
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6688
BstBI TT'CG_AA
Cuts at: 2007 5492 2007
Size: 3485 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6688
BstXI CCAn_nnnn'nTGG
Cuts at: 2029 2831 2029
Size: 802 5886
BstYI r'GATC_y
Cuts at: 1972 2677 3960 4018 4385 4770 4787 6239 6325
Size: 705 1283 58 367 385 17 1452 86
Cuts at: 6325 6337 6683 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1283 705 385 367 346 86
58 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2107 2403 513
Size: 552 957 85 296 4798
Fragments arranged by size:
4798 957 552 296 85
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2958 487
Size: 683 56 1732 4217
Fragments arranged by size:
4217 1732 683 56
BtsI GCAGTG_nn'
Cuts at: 2459 3225 3688 4204 4998 5018 2459
Size: 766 463 516 794 20 4129
Fragments arranged by size:
4129 794 766 516 463 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6688
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6653
DraI TTT'AAA
Cuts at: 3992 3992
Size: 6688
DraIII CAC_nnn'GTG
Cuts at: 2454 3601 2454
Size: 1147 5541
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5695 809
Size: 507 4379 1802
Fragments arranged by size:
4379 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3751 3804 4135
Size: 54 1425 50 275 18 1921 53 331
Cuts at: 4135 5024 6653 8
Size: 889 1629 43
Fragments arranged by size:
1921 1629 1425 889 331 275 54 53
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4135 1537
Size: 275 2323 4090
Fragments arranged by size:
4090 2323 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3028 3142 3419 4618 1353
Size: 1675 114 277 1199 3423
Fragments arranged by size:
3423 1675 1199 277 114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2317 5275 5649 5795 387
Size: 1064 138 728 2958 374 146 1280
Fragments arranged by size:
2958 1280 1064 728 374 146 138
EcoICRI GAG'CTC
Cuts at: 2127 2325 3835 2127
Size: 198 1510 4980
Fragments arranged by size:
4980 1510 198
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2575 2904 2978 3303 3643 3710 3797
Size: 393 943 329 74 325 340 67 87
Cuts at: 3797 3920 4751 5348 6135 6403 1239
Size: 123 831 597 787 268 1524
Fragments arranged by size:
1524 943 831 787 597 393 340 329
325 268 123 87 74 67
EcoNI CCTnn'n_nnAGG
Cuts at: 2396 2396
Size: 6688
EcoO109I rG'GnC_Cy
Cuts at: 2057 2262 2263 2828 2934 2980 3070 3071 3158
Size: 205 1 565 106 46 90 1 87
Cuts at: 3158 3297 3485 3498 3571 3739 3971 2057
Size: 139 188 13 73 168 232 4774
Fragments arranged by size:
4774 565 232 205 188 168 139 106
90 87 73 46 13 1 1
EcoRI G'AATT_C
Cuts at: 3997 4375 3997
Size: 378 6310
FspI TGC'GCA
Cuts at: 2744 5194 2744
Size: 2450 4238
HaeII r_GCGC'y
Cuts at: 1678 2312 2414 2547 2670 2838 5835 1678
Size: 634 102 133 123 168 2997 2531
Fragments arranged by size:
2997 2531 634 168 133 123 102
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2624 2656 2999 3031 5329
Size: 32 135 32 1052 32 343 32 2298
Cuts at: 5329 5361 5403 5435 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2298 1052 343 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2383 4032 4278 669
Size: 988 281 445 1649 246 3079
Fragments arranged by size:
3079 1649 988 445 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6688
HpaI GTT'AAC
Cuts at: 4278 4278
Size: 6688
KasI G'GCGC_C
Cuts at: 2308 2834 2308
Size: 526 6162
KpnI G_GTAC'C
Cuts at: 4013 4013
Size: 6688
MfeI C'AATT_G
Cuts at: 4287 4287
Size: 6688
MluI A'CGCG_T
Cuts at: 2151 6564 2151
Size: 4413 2275
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2790 3422 5802 5986 1305
Size: 1485 632 2380 184 2007
Fragments arranged by size:
2380 2007 1485 632 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3753 10
Size: 54 1425 343 1921 2945
Fragments arranged by size:
2945 1921 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2357 3006 4646 5005 5164 518
Size: 648 1191 649 1640 359 159 2042
Fragments arranged by size:
2042 1640 1191 649 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2067 2109 2808 2871 3788 3856 3877 4104
Size: 461 42 699 63 917 68 21 227
Cuts at: 4104 4781 5929 6174 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 917 699 677 461 245 227
68 63 42 21
NaeI GCC'GGC
Cuts at: 2049 2049
Size: 6688
NarI GG'CG_CC
Cuts at: 2309 2835 2309
Size: 526 6162
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5179
Fragments arranged by size:
5179 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6688
NgoMIV G'CCGG_C
Cuts at: 2047 2047
Size: 6688
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6688
NotI GC'GGCC_GC
Cuts at: 4135 4135
Size: 6688
NspI r_CATG'y
Cuts at: 1132 1229 3890 4046 5591 1132
Size: 97 2661 156 1545 2229
Fragments arranged by size:
2661 2229 1545 156 97
PasI CC'CwG_GG
Cuts at: 1348 2313 3067 3266 1348
Size: 965 754 199 4770
Fragments arranged by size:
4770 965 754 199
PciI A'CATG_T
Cuts at: 5587 5587
Size: 6688
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3116 3760 1108
Size: 2008 644 4036
Fragments arranged by size:
4036 2008 644
PmeI GTTT'AAAC
Cuts at: 3992 3992
Size: 6688
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4757 4789 6270 6302 4757
Size: 32 1481 32 5143
Fragments arranged by size:
5143 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2828 2934 3485 3571 2828
Size: 106 551 86 5945
Fragments arranged by size:
5945 551 106 86
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6688
PsiI TTA'TAA
Cuts at: 4258 4258
Size: 6688
PspOMI G'GGCC_C
Cuts at: 2103 2262 2302 2979 3070 3297 3970 2103
Size: 159 40 677 91 227 673 4821
Fragments arranged by size:
4821 677 673 227 159 91 40
PstI C_TGCA'G
Cuts at: 830 3855 3960 4040 830
Size: 3025 105 80 3478
Fragments arranged by size:
3478 3025 105 80
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6688
PvuII CAG'CTG
Cuts at: 2871 3788 2871
Size: 917 5771
SacI G_AGCT'C
Cuts at: 2129 2327 3837 2129
Size: 198 1510 4980
Fragments arranged by size:
4980 1510 198
SacII CC_GC'GG
Cuts at: 2110 2110
Size: 6688
SalI G'TCGA_C
Cuts at: 1936 2381 4030 1936
Size: 445 1649 4594
Fragments arranged by size:
4594 1649 445
SanDI GG'GwC_CC
Cuts at: 3485 3485
Size: 6688
SapI GCTCTTCn'nnn_
Cuts at: 3028 3028
Size: 6688
SbfI CC_TGCA'GG
Cuts at: 4040 4040
Size: 6688
ScaI AGT'ACT
Cuts at: 3812 4936 6648 3812
Size: 1124 1712 3852
Fragments arranged by size:
3852 1712 1124
SfcI C'TryA_G
Cuts at: 826 1045 2938 3851 3956 4036 5171 5852 6043
Size: 219 1893 913 105 80 1135 681 191
Cuts at: 6043 826
Size: 1471
Fragments arranged by size:
1893 1471 1135 913 681 219 191 105
80
SfiI GGCCn_nnn'nGGCC
Cuts at: 3269 3269
Size: 6688
SfoI GGC'GCC
Cuts at: 2310 2836 2310
Size: 526 6162
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6688
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6688
SmaI CCC'GGG
Cuts at: 1872 3969 4015 1872
Size: 2097 46 4545
Fragments arranged by size:
4545 2097 46
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3556 4792 5693 5955 820
Size: 197 926 1613 1236 901 262 1553
Fragments arranged by size:
1613 1553 1236 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3985 4479 493
Size: 3492 494 2702
Fragments arranged by size:
3492 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6688
SphI G_CATG'C
Cuts at: 1132 4046 1132
Size: 2914 3774
SspI AAT'ATT
Cuts at: 5 52 4612 5
Size: 47 4560 2081
Fragments arranged by size:
4560 2081 47
StuI AGG'CCT
Cuts at: 1517 3435 1517
Size: 1918 4770
StyI C'CwwG_G
Cuts at: 513 1065 2022 2579 3430 3567 4127 513
Size: 552 957 557 851 137 560 3074
Fragments arranged by size:
3074 957 851 560 557 552 137
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2939 3352 3961 4715 4732 4885 5070
Size: 1255 1359 413 609 754 17 153 185
Cuts at: 5070 5515 325
Size: 445 1498
Fragments arranged by size:
1498 1359 1255 754 609 445 413 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2184 3810 4934
Size: 274 80 33 51 1443 207 1626 1124
Cuts at: 4934 6646 96
Size: 1712 138
Fragments arranged by size:
1712 1626 1443 1124 274 207 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3074 3106 6648 6680 3074
Size: 32 3542 32 3082
Fragments arranged by size:
3542 3082 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2420 2761 1316
Size: 1104 341 5243
Fragments arranged by size:
5243 1104 341
XbaI T'CTAG_A
Cuts at: 4024 4024
Size: 6688
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2410 2773 2812 3340 2410
Size: 363 39 528 5758
Fragments arranged by size:
5758 528 363 39
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6688
XmaI C'CCGG_G
Cuts at: 1870 3967 4013 1870
Size: 2097 46 4545
Fragments arranged by size:
4545 2097 46
XmnI GAAnn'nnTTC
Cuts at: 4817 4817
Size: 6688
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4496 276
Size: 53 83 186 936 2962 2468
Fragments arranged by size:
2962 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI
BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI
NsiI PacI PfoI PmlI PspXI PsrI RsrII SexAI
SrfI SwaI