Restriction map for
pFN21ASDA0851
(Circular) (Six-base) MAPSORT of: pFN21ASDA0851.seq Check: 3652 from: 1 to: 6490
pFN21ASDA0851 6490 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4300 278
Size: 53 83 186 936 2764 2468
Fragments arranged by size:
2764 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2200 3833 1937
Size: 263 1633 4594
Fragments arranged by size:
4594 1633 263
Acc65I G'GTAC_C
Cuts at: 3811 3811
Size: 6490
AclI AA'CG_TT
Cuts at: 1563 4617 4990 1563
Size: 3054 373 3063
Fragments arranged by size:
3063 3054 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2069 2157 3230 3242 4553 5937 6205 1632
Size: 437 88 1073 12 1311 1384 268 1917
Fragments arranged by size:
1917 1384 1311 1073 437 268 88 12
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6293
AflIII A'CryG_T
Cuts at: 1203 5389 6366 1203
Size: 4186 977 1327
Fragments arranged by size:
4186 1327 977
AgeI A'CCGG_T
Cuts at: 5321 5321
Size: 6490
AhdI GACnn_n'nnGTC
Cuts at: 2128 5219 2128
Size: 3091 3399
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6490
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3000 1184
Size: 1816 4674
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3158 3190 3464 3496 3158
Size: 32 274 32 6152
Fragments arranged by size:
6152 274 32 32
AlwNI CAG_nnn'CTG
Cuts at: 4233 5805 4233
Size: 1572 4918
ApaLI G'TGCA_C
Cuts at: 2753 4547 5703 2753
Size: 1794 1156 3540
Fragments arranged by size:
3540 1794 1156
ApoI r'AATT_y
Cuts at: 1493 2072 2270 2477 3308 3581 3799 4025 4177
Size: 579 198 207 831 273 218 226 152
Cuts at: 4177 6286 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 831 579 273 226 218 207
198 152
AseI AT'TA_AT
Cuts at: 160 1032 2625 5044 160
Size: 872 1593 2419 1606
Fragments arranged by size:
2419 1606 1593 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6490
AvaI C'yCGr_G
Cuts at: 1870 1943 2656 2790 3815 1870
Size: 73 713 134 1025 4545
Fragments arranged by size:
4545 1025 713 134 73
BamHI G'GATC_C
Cuts at: 3820 4187 3820
Size: 367 6123
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3380 3811 5266 618
Size: 325 217 627 1593 431 1455 1842
Fragments arranged by size:
1842 1593 1455 627 431 325 217
BanII G_rGCy'C
Cuts at: 1391 2960 3809 1391
Size: 1569 849 4072
Fragments arranged by size:
4072 1569 849
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2333 2991 3395 928
Size: 971 434 658 404 4023
Fragments arranged by size:
4023 971 658 434 404
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3168 3202 4681 4715 1903
Size: 34 1231 34 1479 34 3678
Fragments arranged by size:
3678 1479 1231 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4382 5592 4382
Size: 1210 5280
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6490
BfrBI ATG'CAT
Cuts at: 2611 2611
Size: 6490
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5101 136
Size: 107 122 71 631 605 3429 1525
Fragments arranged by size:
3429 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6485 6485
Size: 6490
BlpI GC'TnA_GC
Cuts at: 3907 3907
Size: 6490
Bme1580I G_kGCm'C
Cuts at: 1790 2210 2757 4551 5707 1790
Size: 420 547 1794 1156 2573
Fragments arranged by size:
2573 1794 1156 547 420
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6490
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2213 4221 5179
Size: 416 53 83 399 24 796 2008 958
Cuts at: 5179 442
Size: 1753
Fragments arranged by size:
2008 1753 958 796 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6490
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5150 1239
Size: 3911 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2816 2993 4615 5480 5778 2816
Size: 177 1622 865 298 3528
Fragments arranged by size:
3528 1622 865 298 177
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5153 882
Size: 617 3654 2219
Fragments arranged by size:
3654 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3787 4281 493
Size: 711 2583 494 2702
Fragments arranged by size:
2702 2583 711 494
BsaBI GATnn'nnATC
Cuts at: 2651 6484 2651
Size: 3833 2657
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4297 4679 5314 275
Size: 53 83 186 936 2764 382 635 1451
Fragments arranged by size:
2764 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4923 5321 5595 5742 6153 4923
Size: 398 274 147 411 5260
Fragments arranged by size:
5260 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2653 2683 1178
Size: 30 165 30 1250 30 4985
Fragments arranged by size:
4985 1250 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2674 2677 1186
Size: 1488 3 4999
Fragments arranged by size:
4999 1488 3
BseYI C'CCAG_C
Cuts at: 5693 6189 5693
Size: 496 5994
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2538 1163
Size: 573 802 5115
Fragments arranged by size:
5115 802 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3940 4701 5729 1540
Size: 275 186 1939 761 1028 2301
Fragments arranged by size:
2301 1939 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2757 3684 4551 4636 5707 2757
Size: 927 867 85 1071 3540
Fragments arranged by size:
3540 1071 927 867 85
BsmI GAATG_Cn'
Cuts at: 2611 4001 4094 2611
Size: 1390 93 5007
Fragments arranged by size:
5007 1390 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2210 2757 2960 3684 3809 4551 4636
Size: 399 420 547 203 724 125 742 85
Cuts at: 4636 5707 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 724 547 420 399 203
125 85
BspEI T'CCGG_A
Cuts at: 6153 6153
Size: 6490
BspHI T'CATG_A
Cuts at: 4379 6109 4379
Size: 1730 4760
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2216 3845 844
Size: 1372 1629 3489
Fragments arranged by size:
3489 1629 1372
BsrBI CCG'CTC
Cuts at: 1394 1823 4377 1394
Size: 429 2554 3507
Fragments arranged by size:
3507 2554 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3027 4985 5159 65
Size: 1183 1779 1958 174 1396
Fragments arranged by size:
1958 1779 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5134 5321 1700
Size: 110 3324 187 2869
Fragments arranged by size:
3324 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2782 96
Size: 2686 3804
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6490
BssSI C'ACGA_G
Cuts at: 4550 5562 4550
Size: 1012 5478
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6490
BstBI TT'CG_AA
Cuts at: 2007 5294 2007
Size: 3287 3203
BstEII G'GTnAC_C
Cuts at: 1184 2140 1184
Size: 956 5534
BstXI CCAn_nnnn'nTGG
Cuts at: 3576 3576
Size: 6490
BstYI r'GATC_y
Cuts at: 1972 3820 4187 4572 4589 6041 6127 6139 6485
Size: 1848 367 385 17 1452 86 12 346
Cuts at: 6485 1972
Size: 1977
Fragments arranged by size:
1977 1848 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2201 2201
Size: 6490
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4981
Fragments arranged by size:
4981 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2396 487
Size: 683 56 1170 4581
Fragments arranged by size:
4581 1170 683 56
BtsI GCAGTG_nn'
Cuts at: 2843 4006 4800 4820 2843
Size: 1163 794 20 4513
Fragments arranged by size:
4513 1163 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6490
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6455
DraI TTT'AAA
Cuts at: 3794 3794
Size: 6490
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5497 809
Size: 507 4181 1802
Fragments arranged by size:
4181 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3145 3937 4826
Size: 54 1425 50 275 18 1315 792 889
Cuts at: 4826 6455 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1315 889 792 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3937 1537
Size: 275 2125 4090
Fragments arranged by size:
4090 2125 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3689 4420 1353
Size: 2336 731 3423
Fragments arranged by size:
3423 2336 731
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5077 5451 5597 387
Size: 1064 138 3488 374 146 1280
Fragments arranged by size:
3488 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 2157 3230 3242 4553 5150 5937
Size: 393 437 88 1073 12 1311 597 787
Cuts at: 5937 6205 1239
Size: 268 1524
Fragments arranged by size:
1524 1311 1073 787 597 437 393 268
88 12
EcoRI G'AATT_C
Cuts at: 2477 3799 4177 2477
Size: 1322 378 4790
Fragments arranged by size:
4790 1322 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3412 3493 3412
Size: 81 6409
FspI TGC'GCA
Cuts at: 4996 4996
Size: 6490
HaeII r_GCGC'y
Cuts at: 1678 5637 1678
Size: 3959 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2114 2146 2814 2846 3055
Size: 32 135 32 542 32 668 32 209
Cuts at: 3055 3087 5131 5163 5205 5237 1373
Size: 32 2044 32 42 32 2626
Fragments arranged by size:
2626 2044 668 542 209 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3834 4080 669
Size: 988 281 1896 246 3079
Fragments arranged by size:
3079 1896 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2085 2772 3290 748
Size: 1337 687 518 3948
Fragments arranged by size:
3948 1337 687 518
HpaI GTT'AAC
Cuts at: 4080 4080
Size: 6490
KpnI G_GTAC'C
Cuts at: 3815 3815
Size: 6490
MfeI C'AATT_G
Cuts at: 3184 4089 3184
Size: 905 5585
MluI A'CGCG_T
Cuts at: 6366 6366
Size: 6490
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2487 5604 5788 1305
Size: 1182 3117 184 2007
Fragments arranged by size:
3117 2007 1182 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3147 10
Size: 54 1425 343 1315 3353
Fragments arranged by size:
3353 1425 1315 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2760 4448 4807 4966 518
Size: 648 1594 1688 359 159 2042
Fragments arranged by size:
2042 1688 1594 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2047 2177 2455 2515 3906 4583 5731 5976
Size: 441 130 278 60 1391 677 1148 245
Cuts at: 5976 1606
Size: 2120
Fragments arranged by size:
2120 1391 1148 677 441 278 245 130
60
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4981
Fragments arranged by size:
4981 957 552
NdeI CA'TA_TG
Cuts at: 387 2431 387
Size: 2044 4446
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6490
NotI GC'GGCC_GC
Cuts at: 3937 3937
Size: 6490
NsiI A_TGCA'T
Cuts at: 2613 2613
Size: 6490
NspI r_CATG'y
Cuts at: 1132 1229 3281 3848 5393 1132
Size: 97 2052 567 1545 2229
Fragments arranged by size:
2229 2052 1545 567 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6490
PciI A'CATG_T
Cuts at: 5389 5389
Size: 6490
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2195 2221 3154 1108
Size: 1087 26 933 4444
Fragments arranged by size:
4444 1087 933 26
PmeI GTTT'AAAC
Cuts at: 3794 3794
Size: 6490
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4559 4591 6072 6104 4559
Size: 32 1481 32 4945
Fragments arranged by size:
4945 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6490
PsiI TTA'TAA
Cuts at: 2322 2933 3434 3468 4060 2322
Size: 611 501 34 592 4752
Fragments arranged by size:
4752 611 592 501 34
PspXI vC'TCGA_Gb
Cuts at: 2790 2790
Size: 6490
PstI C_TGCA'G
Cuts at: 830 3842 830
Size: 3012 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6490
PvuII CAG'CTG
Cuts at: 2047 2177 2047
Size: 130 6360
SalI G'TCGA_C
Cuts at: 1936 3832 1936
Size: 1896 4594
SapI GCTCTTCn'nnn_
Cuts at: 3689 3689
Size: 6490
SbfI CC_TGCA'GG
Cuts at: 3842 3842
Size: 6490
ScaI AGT'ACT
Cuts at: 4738 6450 4738
Size: 1712 4778
SfcI C'TryA_G
Cuts at: 826 1045 3838 4973 5654 5845 826
Size: 219 2793 1135 681 191 1471
Fragments arranged by size:
2793 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6490
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6490
SmaI CCC'GGG
Cuts at: 1872 3817 1872
Size: 1945 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2656 2790 2831 2972 4594 5495
Size: 197 926 713 134 41 141 1622 901
Cuts at: 5495 5757 820
Size: 262 1553
Fragments arranged by size:
1622 1553 926 901 713 262 197 141
134 41
SnaBI TAC'GTA
Cuts at: 493 3787 4281 493
Size: 3294 494 2702
Fragments arranged by size:
3294 2702 494
SpeI A'CTAG_T
Cuts at: 152 2468 152
Size: 2316 4174
SphI G_CATG'C
Cuts at: 1132 3848 1132
Size: 2716 3774
SspI AAT'ATT
Cuts at: 5 52 2193 4414 5
Size: 47 2141 2221 2081
Fragments arranged by size:
2221 2141 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6490
StyI C'CwwG_G
Cuts at: 513 1065 2022 3003 3569 3929 513
Size: 552 957 981 566 360 3074
Fragments arranged by size:
3074 981 957 566 552 360
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4517 4534 4687 4872 5317 325
Size: 1255 2937 17 153 185 445 1498
Fragments arranged by size:
2937 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2782 2835 4736
Size: 274 80 33 51 1443 805 53 1901
Cuts at: 4736 6448 96
Size: 1712 138
Fragments arranged by size:
1901 1712 1443 805 274 138 80 53
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6450 6482 6450
Size: 32 6458
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6490
XbaI T'CTAG_A
Cuts at: 3199 3826 3199
Size: 627 5863
XhoI C'TCGA_G
Cuts at: 1943 2656 2790 1943
Size: 713 134 5643
Fragments arranged by size:
5643 713 134
XmaI C'CCGG_G
Cuts at: 1870 3815 1870
Size: 1945 4545
XmnI GAAnn'nnTTC
Cuts at: 2707 3526 4619 2707
Size: 819 1093 4578
Fragments arranged by size:
4578 1093 819
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4298 276
Size: 53 83 186 936 2764 2468
Fragments arranged by size:
2764 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspOMI PsrI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI