Restriction map for
pFN21ASDA0846
(Circular) (Six-base) MAPSORT of: pFN21ASDA0846.seq Check: 881 from: 1 to: 6796
pFN21ASDA0846 6796 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4606 278
Size: 53 83 186 936 3070 2468
Fragments arranged by size:
3070 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4020 4139 1937
Size: 2083 119 4594
Fragments arranged by size:
4594 2083 119
Acc65I G'GTAC_C
Cuts at: 3217 4117 3217
Size: 900 5896
AclI AA'CG_TT
Cuts at: 1563 4923 5296 1563
Size: 3360 373 3063
Fragments arranged by size:
3360 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2291 2705 2799 3150 4859 6243 6511 1632
Size: 659 414 94 351 1709 1384 268 1917
Fragments arranged by size:
1917 1709 1384 659 414 351 268 94
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6599
AflIII A'CryG_T
Cuts at: 1203 2362 5695 6672 1203
Size: 1159 3333 977 1327
Fragments arranged by size:
3333 1327 1159 977
AgeI A'CCGG_T
Cuts at: 5627 5627
Size: 6796
AhdI GACnn_n'nnGTC
Cuts at: 3904 5525 3904
Size: 1621 5175
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6796
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6796
AlwNI CAG_nnn'CTG
Cuts at: 3887 4539 6111 3887
Size: 652 1572 4572
Fragments arranged by size:
4572 1572 652
ApaLI G'TGCA_C
Cuts at: 2071 4853 6009 2071
Size: 2782 1156 2858
Fragments arranged by size:
2858 2782 1156
ApoI r'AATT_y
Cuts at: 1493 2228 2324 2731 3172 4105 4331 4483 6592
Size: 735 96 407 441 933 226 152 2109
Cuts at: 6592 1493
Size: 1697
Fragments arranged by size:
2109 1697 933 735 441 407 226 152
96
AseI AT'TA_AT
Cuts at: 160 1032 5350 160
Size: 872 4318 1606
Fragments arranged by size:
4318 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6796
AvaI C'yCGr_G
Cuts at: 1870 1943 3663 3901 4121 1870
Size: 73 1720 238 220 4545
Fragments arranged by size:
4545 1720 238 220 73
BamHI G'GATC_C
Cuts at: 4126 4493 4126
Size: 367 6429
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3217 3667 4117 5572 618
Size: 325 217 627 1430 450 450 1455 1842
Fragments arranged by size:
1842 1455 1430 627 450 450 325 217
BanII G_rGCy'C
Cuts at: 1391 2481 4115 1391
Size: 1090 1634 4072
Fragments arranged by size:
4072 1634 1090
BbeI G_GCGC'C
Cuts at: 3671 3671
Size: 6796
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5825
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3533 3567 4987 5021 1903
Size: 34 1596 34 1420 34 3678
Fragments arranged by size:
3678 1596 1420 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2446 3578 4688 5898 2446
Size: 1132 1110 1210 3344
Fragments arranged by size:
3344 1210 1132 1110
BclI T'GATC_A
Cuts at: 1548 3090 3316 1548
Size: 1542 226 5028
Fragments arranged by size:
5028 1542 226
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5407 136
Size: 107 122 71 631 605 3735 1525
Fragments arranged by size:
3735 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2241 3819 6791 2241
Size: 1578 2972 2246
Fragments arranged by size:
2972 2246 1578
BlpI GC'TnA_GC
Cuts at: 2493 4213 2493
Size: 1720 5076
Bme1580I G_kGCm'C
Cuts at: 1790 2075 4031 4857 6013 1790
Size: 285 1956 826 1156 2573
Fragments arranged by size:
2573 1956 1156 826 285
BmgBI CAC'GTC
Cuts at: 1318 2365 1318
Size: 1047 5749
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4527 5485 442
Size: 416 53 83 399 24 3110 958 1753
Fragments arranged by size:
3110 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6796
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2872 2872
Size: 6796
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2232 2495 2531 2597 3182 3245 3771 5456
Size: 993 263 36 66 585 63 526 1685
Cuts at: 5456 1239
Size: 2579
Fragments arranged by size:
2579 1685 993 585 526 263 66 63
36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3512 3697 4921 5786 6084 3512
Size: 185 1224 865 298 4224
Fragments arranged by size:
4224 1224 865 298 185
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2789 2860 4058 5459 882
Size: 617 1290 71 1198 1401 2219
Fragments arranged by size:
2219 1401 1290 1198 617 71
BsaAI yAC'GTr
Cuts at: 493 1204 4093 4587 493
Size: 711 2889 494 2702
Fragments arranged by size:
2889 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 6790 6790
Size: 6796
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3200 3668 4603 4985
Size: 53 83 186 936 1667 468 935 382
Cuts at: 4985 5620 275
Size: 635 1451
Fragments arranged by size:
1667 1451 936 935 635 468 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5229 5627 5901 6048 6459 5229
Size: 398 274 147 411 5566
Fragments arranged by size:
5566 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3005 3035 1178
Size: 30 165 30 1602 30 4939
Fragments arranged by size:
4939 1602 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2848 2999 1186
Size: 1662 151 4983
Fragments arranged by size:
4983 1662 151
BseYI C'CCAG_C
Cuts at: 2667 3721 3891 5999 6495 2667
Size: 1054 170 2108 496 2968
Fragments arranged by size:
2968 2108 1054 496 170
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6223
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4246 5007 6035 1540
Size: 275 186 2245 761 1028 2301
Fragments arranged by size:
2301 2245 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2075 2499 4857 4942 6013 2075
Size: 424 2358 85 1071 2858
Fragments arranged by size:
2858 2358 1071 424 85
BsmI GAATG_Cn'
Cuts at: 2388 3061 4307 4400 2388
Size: 673 1246 93 4784
Fragments arranged by size:
4784 1246 673 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2075 2481 2499 4031 4115 4857 4942
Size: 399 285 406 18 1532 84 742 85
Cuts at: 4942 6013 1391
Size: 1071 2174
Fragments arranged by size:
2174 1532 1071 742 406 399 285 85
84 18
BspEI T'CCGG_A
Cuts at: 6459 6459
Size: 6796
BspHI T'CATG_A
Cuts at: 2253 4685 6415 2253
Size: 2432 1730 2634
Fragments arranged by size:
2634 2432 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3105 4151 844
Size: 2261 1046 3489
Fragments arranged by size:
3489 2261 1046
BsrBI CCG'CTC
Cuts at: 1394 1823 4683 1394
Size: 429 2860 3507
Fragments arranged by size:
3507 2860 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2230 5291 5465 65
Size: 1183 982 3061 174 1396
Fragments arranged by size:
3061 1396 1183 982 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5440 5627 1700
Size: 110 3630 187 2869
Fragments arranged by size:
3630 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6796
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6796
BssSI C'ACGA_G
Cuts at: 4856 5868 4856
Size: 1012 5784
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2874 794
Size: 2080 4716
BstBI TT'CG_AA
Cuts at: 2007 5600 2007
Size: 3593 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6796
BstXI CCAn_nnnn'nTGG
Cuts at: 2173 2488 3796 2173
Size: 315 1308 5173
Fragments arranged by size:
5173 1308 315
BstYI r'GATC_y
Cuts at: 1972 2241 2296 2532 3819 4126 4493 4878 4895
Size: 269 55 236 1287 307 367 385 17
Cuts at: 4895 6347 6433 6445 6791 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1287 385 367 346 307 269
236 86 55 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2566 3261 513
Size: 552 957 544 695 4048
Fragments arranged by size:
4048 957 695 552 544
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2919 487
Size: 683 56 1693 4364
Fragments arranged by size:
4364 1693 683 56
BtsI GCAGTG_nn'
Cuts at: 2757 3688 3695 3756 4312 5106 5126 2757
Size: 931 7 61 556 794 20 4427
Fragments arranged by size:
4427 931 794 556 61 20 7
ClaI AT'CG_AT
Cuts at: 1554 2603 1554
Size: 1049 5747
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3583 3618 529
Size: 35 3019 35 3707
Fragments arranged by size:
3707 3019 35 35
DraI TTT'AAA
Cuts at: 4100 4100
Size: 6796
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3263 5803 809
Size: 507 1947 2540 1802
Fragments arranged by size:
2540 1947 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4243 5132 6761
Size: 54 1425 50 275 18 2413 889 1629
Cuts at: 6761 8
Size: 43
Fragments arranged by size:
2413 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4243 1537
Size: 275 2431 4090
Fragments arranged by size:
4090 2431 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2407 3814 4056 4726 1353
Size: 1054 1407 242 670 3423
Fragments arranged by size:
3423 1407 1054 670 242
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5383 5757 5903 387
Size: 1064 138 3794 374 146 1280
Fragments arranged by size:
3794 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2232 2291 2495 2531 2597 2705 2799
Size: 393 600 59 204 36 66 108 94
Cuts at: 2799 3150 3182 3245 3771 4859 5456 6243 6511
Size: 351 32 63 526 1088 597 787 268
Cuts at: 6511 1239
Size: 1524
Fragments arranged by size:
1524 1088 787 600 597 526 393 351
268 204 108 94 66 63 59 36
32
EcoO109I rG'GnC_Cy
Cuts at: 3906 3906
Size: 6796
EcoRI G'AATT_C
Cuts at: 4105 4483 4105
Size: 378 6418
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3553 3553
Size: 6796
FspI TGC'GCA
Cuts at: 5302 5302
Size: 6796
HaeII r_GCGC'y
Cuts at: 1678 3671 5943 1678
Size: 1993 2272 2531
Fragments arranged by size:
2531 2272 1993
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2226 2258 2504 2536 2788
Size: 32 135 32 654 32 246 32 252
Cuts at: 2788 2820 3508 3540 3670 3702 3890 3922 5437
Size: 32 688 32 130 32 188 32 1515
Cuts at: 5437 5469 5511 5543 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1515 688 654 252 246 188 135
130 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2345 4140 4386 669
Size: 988 281 407 1795 246 3079
Fragments arranged by size:
3079 1795 988 407 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6796
HpaI GTT'AAC
Cuts at: 4386 4386
Size: 6796
KasI G'GCGC_C
Cuts at: 3667 3667
Size: 6796
KpnI G_GTAC'C
Cuts at: 3221 4121 3221
Size: 900 5896
MfeI C'AATT_G
Cuts at: 3610 4395 3610
Size: 785 6011
MluI A'CGCG_T
Cuts at: 6672 6672
Size: 6796
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2612 2886 5910 6094 1305
Size: 1307 274 3024 184 2007
Fragments arranged by size:
3024 2007 1307 274 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4974
Fragments arranged by size:
4974 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2084 2744 3324 4005 4754 5113 5272
Size: 648 918 660 580 681 749 359 159
Cuts at: 5272 518
Size: 2042
Fragments arranged by size:
2042 918 749 681 660 648 580 359
159
MspA1I CmG'CkG
Cuts at: 1606 4212 4889 6037 6282 1606
Size: 2606 677 1148 245 2120
Fragments arranged by size:
2606 2120 1148 677 245
NarI GG'CG_CC
Cuts at: 3668 3668
Size: 6796
NcoI C'CATG_G
Cuts at: 513 1065 2022 2566 513
Size: 552 957 544 4743
Fragments arranged by size:
4743 957 552 544
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6796
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6796
NotI GC'GGCC_GC
Cuts at: 4243 4243
Size: 6796
NspI r_CATG'y
Cuts at: 1132 1229 2197 4004 4154 5699 1132
Size: 97 968 1807 150 1545 2229
Fragments arranged by size:
2229 1807 1545 968 150 97
PasI CC'CwG_GG
Cuts at: 1348 3993 1348
Size: 2645 4151
PciI A'CATG_T
Cuts at: 5695 5695
Size: 6796
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2644 1108
Size: 1536 5260
PmeI GTTT'AAAC
Cuts at: 4100 4100
Size: 6796
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4865 4897 6378 6410 4865
Size: 32 1481 32 5251
Fragments arranged by size:
5251 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3906 3906
Size: 6796
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6796
PsiI TTA'TAA
Cuts at: 4366 4366
Size: 6796
PstI C_TGCA'G
Cuts at: 830 2284 3054 3653 4148 830
Size: 1454 770 599 495 3478
Fragments arranged by size:
3478 1454 770 599 495
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6796
SalI G'TCGA_C
Cuts at: 1936 4138 1936
Size: 2202 4594
SanDI GG'GwC_CC
Cuts at: 3906 3906
Size: 6796
SapI GCTCTTCn'nnn_
Cuts at: 3814 3814
Size: 6796
SbfI CC_TGCA'GG
Cuts at: 3653 4148 3653
Size: 495 6301
ScaI AGT'ACT
Cuts at: 5044 6756 5044
Size: 1712 5084
SfcI C'TryA_G
Cuts at: 826 1045 2280 3050 3649 3833 4144 5279 5960
Size: 219 1235 770 599 184 311 1135 681
Cuts at: 5960 6151 826
Size: 191 1471
Fragments arranged by size:
1471 1235 1135 770 681 599 311 219
191 184
SfoI GGC'GCC
Cuts at: 3669 3669
Size: 6796
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6796
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6796
SmaI CCC'GGG
Cuts at: 1872 3665 4123 1872
Size: 1793 458 4545
Fragments arranged by size:
4545 1793 458
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3527 3676 4900 5801 6063 820
Size: 197 926 1584 149 1224 901 262 1553
Fragments arranged by size:
1584 1553 1224 926 901 262 197 149
SnaBI TAC'GTA
Cuts at: 493 4093 4587 493
Size: 3600 494 2702
Fragments arranged by size:
3600 2702 494
SpeI A'CTAG_T
Cuts at: 152 3040 152
Size: 2888 3908
SphI G_CATG'C
Cuts at: 1132 4004 4154 1132
Size: 2872 150 3774
Fragments arranged by size:
3774 2872 150
SrfI GCCC'GGGC
Cuts at: 3665 3665
Size: 6796
SspI AAT'ATT
Cuts at: 5 52 4720 5
Size: 47 4668 2081
Fragments arranged by size:
4668 2081 47
StuI AGG'CCT
Cuts at: 1517 2763 2891 1517
Size: 1246 128 5422
Fragments arranged by size:
5422 1246 128
StyI C'CwwG_G
Cuts at: 513 1065 2022 2038 2566 2592 2926 4235 513
Size: 552 957 16 528 26 334 1309 3074
Fragments arranged by size:
3074 1309 957 552 528 334 26 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3875 4823 4840 4993 5178 5623 325
Size: 1255 2295 948 17 153 185 445 1498
Fragments arranged by size:
2295 1498 1255 948 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2932 5042 6754
Size: 274 80 33 51 1443 955 2110 1712
Cuts at: 6754 96
Size: 138
Fragments arranged by size:
2110 1712 1443 955 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3005 3037 6756 6788 3005
Size: 32 3719 32 3013
Fragments arranged by size:
3719 3013 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6796
XbaI T'CTAG_A
Cuts at: 4132 4132
Size: 6796
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3211 3211
Size: 6796
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6796
XmaI C'CCGG_G
Cuts at: 1870 3663 4121 1870
Size: 1793 458 4545
Fragments arranged by size:
4545 1793 458
XmnI GAAnn'nnTTC
Cuts at: 2184 4925 2184
Size: 2741 4055
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4604 276
Size: 53 83 186 936 3070 2468
Fragments arranged by size:
3070 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SexAI SfiI SwaI