Restriction map for
pFN21ASDA0845
(Circular) (Six-base) MAPSORT of: pFN21ASDA0845.seq Check: 2470 from: 1 to: 7807
pFN21ASDA0845 7807 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2550 2550
Size: 7807
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2183 2828 5617 278
Size: 53 83 186 936 647 645 2789 2468
Fragments arranged by size:
2789 2468 936 647 645 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5150 1937
Size: 3213 4594
Acc65I G'GTAC_C
Cuts at: 5128 5128
Size: 7807
AclI AA'CG_TT
Cuts at: 1563 5934 6307 1563
Size: 4371 373 3063
Fragments arranged by size:
4371 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2831 3444 3456 4469 4752 5870 7254 7522
Size: 1199 613 12 1013 283 1118 1384 268
Cuts at: 7522 1632
Size: 1917
Fragments arranged by size:
1917 1384 1199 1118 1013 613 283 268
12
AfeI AGC'GCT
Cuts at: 3059 3059
Size: 7807
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7610
AflIII A'CryG_T
Cuts at: 1203 6706 7683 1203
Size: 5503 977 1327
Fragments arranged by size:
5503 1327 977
AgeI A'CCGG_T
Cuts at: 6638 6638
Size: 7807
AhdI GACnn_n'nnGTC
Cuts at: 2824 2920 6536 2824
Size: 96 3616 4095
Fragments arranged by size:
4095 3616 96
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7807
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7807
AlwNI CAG_nnn'CTG
Cuts at: 2332 2689 3121 3184 5550 7122 2332
Size: 357 432 63 2366 1572 3017
Fragments arranged by size:
3017 2366 1572 432 357 63
ApaLI G'TGCA_C
Cuts at: 5864 7020 5864
Size: 1156 6651
ApoI r'AATT_y
Cuts at: 1493 3306 4379 5116 5342 5494 7603 1493
Size: 1813 1073 737 226 152 2109 1697
Fragments arranged by size:
2109 1813 1697 1073 737 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6361 160
Size: 872 5329 1606
Fragments arranged by size:
5329 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7807
AvaI C'yCGr_G
Cuts at: 1870 1943 2546 3764 4238 5132 1870
Size: 73 603 1218 474 894 4545
Fragments arranged by size:
4545 1218 894 603 474 73
BamHI G'GATC_C
Cuts at: 5137 5504 5137
Size: 367 7440
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2057 2228 2715 2768 4630
Size: 325 217 627 270 171 487 53 1862
Cuts at: 4630 5128 6583 618
Size: 498 1455 1842
Fragments arranged by size:
1862 1842 1455 627 498 487 325 270
217 171 53
BanII G_rGCy'C
Cuts at: 1391 2192 2765 4554 5126 1391
Size: 801 573 1789 572 4072
Fragments arranged by size:
4072 1789 801 573 572
BbeI G_GCGC'C
Cuts at: 2061 2232 2719 2772 2061
Size: 171 487 53 7096
Fragments arranged by size:
7096 487 171 53
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4800 5085 928
Size: 971 2901 285 3650
Fragments arranged by size:
3650 2901 971 285
BbvCI CC'TCA_GC
Cuts at: 3134 3606 3750 3810 3870 3912 3134
Size: 472 144 60 60 42 7029
Fragments arranged by size:
7029 472 144 60 60 42
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5998 6032 1903
Size: 34 4061 34 3678
Fragments arranged by size:
4061 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5699 6909 5699
Size: 1210 6597
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7807
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2400 2612 2654
Size: 107 122 71 631 605 728 212 42
Cuts at: 2654 2777 6418 136
Size: 123 3641 1525
Fragments arranged by size:
3641 1525 728 631 605 212 123 122
107 71 42
BglII A'GATC_T
Cuts at: 7802 7802
Size: 7807
BlpI GC'TnA_GC
Cuts at: 2261 2959 3129 5224 2261
Size: 698 170 2095 4844
Fragments arranged by size:
4844 2095 698 170
Bme1580I G_kGCm'C
Cuts at: 1790 5868 7024 1790
Size: 4078 1156 2573
Fragments arranged by size:
4078 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2821 1318
Size: 1503 6304
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3836 3878 4040
Size: 416 53 83 399 24 2419 42 162
Cuts at: 4040 4082 4124 4166 4208 4250 4502 5538 6496
Size: 42 42 42 42 42 252 1036 958
Cuts at: 6496 442
Size: 1753
Fragments arranged by size:
2419 1753 1036 958 416 399 252 162
83 53 42 42 42 42 42 42
24
BmtI G_CTAG'C
Cuts at: 1056 2108 1056
Size: 1052 6755
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2137 2396 2417 2549 3038 3068 3089 3663
Size: 898 259 21 132 489 30 21 574
Cuts at: 3663 3729 3807 3891 4314 4329 5017 5046 6467
Size: 66 78 84 423 15 688 29 1421
Cuts at: 6467 1239
Size: 2579
Fragments arranged by size:
2579 1421 898 688 574 489 423 259
132 84 78 66 30 29 21 21
15
Bpu10I CC'TnA_GC
Cuts at: 3134 3606 3750 3810 3870 3912 3134
Size: 472 144 60 60 42 7029
Fragments arranged by size:
7029 472 144 60 60 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2221 5932 6797 7095 2221
Size: 3711 865 298 2933
Fragments arranged by size:
3711 2933 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3404 6470 882
Size: 617 1905 3066 2219
Fragments arranged by size:
3066 2219 1905 617
BsaAI yAC'GTr
Cuts at: 493 1204 2563 5104 5598 493
Size: 711 1359 2541 494 2702
Fragments arranged by size:
2702 2541 1359 711 494
BsaBI GATnn'nnATC
Cuts at: 7801 7801
Size: 7807
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2058 2180 2229 2716
Size: 53 83 186 936 525 122 49 487
Cuts at: 2716 2769 2805 2825 2989 5614 5996 6631 275
Size: 53 36 20 164 2625 382 635 1451
Fragments arranged by size:
2625 1451 936 635 525 487 382 186
164 122 83 53 53 49 36 20
BsaWI w'CCGG_w
Cuts at: 4894 6240 6638 6912 7059 7470 4894
Size: 1346 398 274 147 411 5231
Fragments arranged by size:
5231 1346 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3080 3110 3514 3544 4406
Size: 30 165 30 1677 30 404 30 862
Cuts at: 4406 4436 1178
Size: 30 4549
Fragments arranged by size:
4549 1677 862 404 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2140 2615 2711 2906 2984 3422 3458 3476
Size: 954 475 96 195 78 438 36 18
Cuts at: 3476 3479 3512 4427 4532 4535 4616 4640 4679
Size: 3 33 915 105 3 81 24 39
Cuts at: 4679 4931 4952 1186
Size: 252 21 4041
Fragments arranged by size:
4041 954 915 475 438 252 195 105
96 81 78 39 36 33 24 21
18 3 3
BseYI C'CCAG_C
Cuts at: 2053 2207 3162 3535 3643 3781 4862 7010 7506
Size: 154 955 373 108 138 1081 2148 496
Cuts at: 7506 2053
Size: 2354
Fragments arranged by size:
2354 2148 1081 955 496 373 154 138
108
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2891 1163
Size: 573 1155 6079
Fragments arranged by size:
6079 1155 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2341 5257 6018 7046 1540
Size: 275 186 340 2916 761 1028 2301
Fragments arranged by size:
2916 2301 1028 761 340 275 186
BsiHKAI G_wGCw'C
Cuts at: 2192 2541 2879 5868 5953 7024 2192
Size: 349 338 2989 85 1071 2975
Fragments arranged by size:
2989 2975 1071 349 338 85
BsmI GAATG_Cn'
Cuts at: 5318 5411 5318
Size: 93 7714
BsmBI CGTCTCn'nnnn_
Cuts at: 2789 2789
Size: 7807
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2192 2541 2765 2879 4554 5126 5868
Size: 399 402 349 224 114 1675 572 742
Cuts at: 5868 5953 7024 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1675 1071 742 572 402 399 349
224 114 85
BspEI T'CCGG_A
Cuts at: 7470 7470
Size: 7807
BspHI T'CATG_A
Cuts at: 5696 7426 5696
Size: 1730 6077
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2550 2676 2718 3192 4687 5162 844
Size: 1706 126 42 474 1495 475 3489
Fragments arranged by size:
3489 1706 1495 475 474 126 42
BsrBI CCG'CTC
Cuts at: 1394 1823 2439 5694 1394
Size: 429 616 3255 3507
Fragments arranged by size:
3507 3255 616 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3090 6302 6476 65
Size: 1183 1842 3212 174 1396
Fragments arranged by size:
3212 1842 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2985 3601 3805 3865 6451 6638 1700
Size: 110 1175 616 204 60 2586 187 2869
Fragments arranged by size:
2869 2586 1175 616 204 187 110 60
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7807
BssHII G'CGCG_C
Cuts at: 1925 2510 2622 2630 2657 2847 1925
Size: 585 112 8 27 190 6885
Fragments arranged by size:
6885 585 190 112 27 8
BssSI C'ACGA_G
Cuts at: 2167 5867 6879 2167
Size: 3700 1012 3095
Fragments arranged by size:
3700 3095 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2689 794
Size: 1895 5912
BstBI TT'CG_AA
Cuts at: 2007 6611 2007
Size: 4604 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7807
BstXI CCAn_nnnn'nTGG
Cuts at: 3016 3016
Size: 7807
BstYI r'GATC_y
Cuts at: 1972 2754 4479 5137 5504 5889 5906 7358 7444
Size: 782 1725 658 367 385 17 1452 86
Cuts at: 7444 7456 7802 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1725 1452 782 658 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3708 4935 5007 3708
Size: 1227 72 6508
Fragments arranged by size:
6508 1227 72
BtgI C'CryG_G
Cuts at: 513 1065 2218 513
Size: 552 1153 6102
Fragments arranged by size:
6102 1153 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2539 487
Size: 683 56 1313 5755
Fragments arranged by size:
5755 1313 683 56
BtsI GCAGTG_nn'
Cuts at: 2314 5323 6117 6137 2314
Size: 3009 794 20 3984
Fragments arranged by size:
3984 3009 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7807
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7772
DraI TTT'AAA
Cuts at: 5111 5111
Size: 7807
DraIII CAC_nnn'GTG
Cuts at: 2309 2869 3147 2309
Size: 560 278 6969
Fragments arranged by size:
6969 560 278
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2179 6814 809
Size: 507 863 4635 1802
Fragments arranged by size:
4635 1802 863 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2295 2749 3156
Size: 54 1425 50 275 18 465 454 407
Cuts at: 3156 4824 5254 6143 7772 8
Size: 1668 430 889 1629 43
Fragments arranged by size:
1668 1629 1425 889 465 454 430 407
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5254 1537
Size: 275 3442 4090
Fragments arranged by size:
4090 3442 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2724 3243 3339 3435 3489 3537 3582 3684
Size: 1371 519 96 96 54 48 45 102
Cuts at: 3684 3786 4008 4488 4566 4719 5737 1353
Size: 102 222 480 78 153 1018 3423
Fragments arranged by size:
3423 1371 1018 519 480 222 153 102
102 96 96 78 54 48 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2128 2188 6394 6768 6914 387
Size: 1064 138 539 60 4206 374 146 1280
Fragments arranged by size:
4206 1280 1064 539 374 146 138 60
EcoICRI GAG'CTC
Cuts at: 2190 2190
Size: 7807
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2137 2396 2417 2549 2831 3038 3068
Size: 393 505 259 21 132 282 207 30
Cuts at: 3068 3089 3444 3456 3663 3729 3807 3891 4314
Size: 21 355 12 207 66 78 84 423
Cuts at: 4314 4329 4469 4752 5017 5046 5870 6467 7254
Size: 15 140 283 265 29 824 597 787
Cuts at: 7254 7522 1239
Size: 268 1524
Fragments arranged by size:
1524 824 787 597 505 423 393 355
283 282 268 265 259 207 207 140
132 84 78 66 30 29 21 21
15 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2531 2546 4451 2531
Size: 15 1905 5887
Fragments arranged by size:
5887 1905 15
EcoO109I rG'GnC_Cy
Cuts at: 3181 4365 4465 3181
Size: 1184 100 6523
Fragments arranged by size:
6523 1184 100
EcoRI G'AATT_C
Cuts at: 5116 5494 5116
Size: 378 7429
FspI TGC'GCA
Cuts at: 6313 6313
Size: 7807
HaeII r_GCGC'y
Cuts at: 1678 2061 2232 2333 2417 2575 2690 2719 2772
Size: 383 171 101 84 158 115 29 53
Cuts at: 2772 2834 3061 6954 1678
Size: 62 227 3893 2531
Fragments arranged by size:
3893 2531 383 227 171 158 115 101
84 62 53 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2810 2842 2906 2938 3080
Size: 32 135 32 1238 32 64 32 142
Cuts at: 3080 3112 3536 3568 3581 3599 3613 3631 3683
Size: 32 424 32 13 18 14 18 52
Cuts at: 3683 3701 3715 3733 3743 3775 3785 3803 3817
Size: 18 14 18 10 32 10 18 14
Cuts at: 3817 3835 3863 3895 3905 3937 4007 4039 4310
Size: 18 28 32 10 32 70 32 271
Cuts at: 4310 4342 4406 4438 4487 4519 6448 6480 6522
Size: 32 64 32 49 32 1929 32 42
Cuts at: 6522 6554 1373
Size: 32 2626
Fragments arranged by size:
2626 1929 1238 424 271 142 135 70
64 64 52 49 42 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 28 18 18 18
18 18 18 14 14 14 13 10
10 10
HincII GTy'rAC
Cuts at: 669 1657 1938 5151 5397 669
Size: 988 281 3213 246 3079
Fragments arranged by size:
3213 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7807
HpaI GTT'AAC
Cuts at: 5397 5397
Size: 7807
KasI G'GCGC_C
Cuts at: 2057 2228 2715 2768 2057
Size: 171 487 53 7096
Fragments arranged by size:
7096 487 171 53
KpnI G_GTAC'C
Cuts at: 5132 5132
Size: 7807
MfeI C'AATT_G
Cuts at: 5406 5406
Size: 7807
MluI A'CGCG_T
Cuts at: 7683 7683
Size: 7807
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2904 3236 3968 6921 7105 1305
Size: 1599 332 732 2953 184 2007
Fragments arranged by size:
2953 2007 1599 732 332 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2297 4826 10
Size: 54 1425 343 465 2529 2991
Fragments arranged by size:
2991 2529 1425 465 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3332 5765 6124 6283 518
Size: 648 2166 2433 359 159 2042
Fragments arranged by size:
2433 2166 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2074 2144 2157 2323 2374 2995 3121 3166
Size: 468 70 13 166 51 621 126 45
Cuts at: 3166 3749 3911 5223 5900 7048 7293 1606
Size: 583 162 1312 677 1148 245 2120
Fragments arranged by size:
2120 1312 1148 677 621 583 468 245
166 162 126 70 51 45 13
NarI GG'CG_CC
Cuts at: 2058 2229 2716 2769 2058
Size: 171 487 53 7096
Fragments arranged by size:
7096 487 171 53
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7255
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7807
NheI G'CTAG_C
Cuts at: 1052 2104 1052
Size: 1052 6755
NotI GC'GGCC_GC
Cuts at: 5254 5254
Size: 7807
NspI r_CATG'y
Cuts at: 1132 1229 5165 6710 1132
Size: 97 3936 1545 2229
Fragments arranged by size:
3936 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7807
PciI A'CATG_T
Cuts at: 6706 6706
Size: 7807
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7807
PfoI T'CCnGG_A
Cuts at: 3237 3237
Size: 7807
PmeI GTTT'AAAC
Cuts at: 5111 5111
Size: 7807
PmlI CAC'GTG
Cuts at: 2563 2563
Size: 7807
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2882 2914 5876 5908 7389 7421 2882
Size: 32 2962 32 1481 32 3268
Fragments arranged by size:
3268 2962 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3181 4365 3181
Size: 1184 6623
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7807
PsiI TTA'TAA
Cuts at: 5377 5377
Size: 7807
PspXI vC'TCGA_Gb
Cuts at: 2546 2546
Size: 7807
PstI C_TGCA'G
Cuts at: 830 2328 2673 2694 2886 3000 4375 5159 830
Size: 1498 345 21 192 114 1375 784 3478
Fragments arranged by size:
3478 1498 1375 784 345 192 114 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7807
PvuII CAG'CTG
Cuts at: 2323 2374 2995 3121 3166 3749 3911 2323
Size: 51 621 126 45 583 162 6219
Fragments arranged by size:
6219 621 583 162 126 51 45
SacI G_AGCT'C
Cuts at: 2192 2192
Size: 7807
SalI G'TCGA_C
Cuts at: 1936 5149 1936
Size: 3213 4594
SbfI CC_TGCA'GG
Cuts at: 5159 5159
Size: 7807
ScaI AGT'ACT
Cuts at: 6055 7767 6055
Size: 1712 6095
SfcI C'TryA_G
Cuts at: 826 1045 2324 2669 2690 2882 2996 4371 5155
Size: 219 1279 345 21 192 114 1375 784
Cuts at: 5155 6290 6971 7162 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1375 1279 1135 784 681 345 219
192 191 114 21
SfoI GGC'GCC
Cuts at: 2059 2230 2717 2770 2059
Size: 171 487 53 7096
Fragments arranged by size:
7096 487 171 53
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7807
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7807
SmaI CCC'GGG
Cuts at: 1872 5134 1872
Size: 3262 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2236 2546 5911 6812 7074 820
Size: 197 926 293 310 3365 901 262 1553
Fragments arranged by size:
3365 1553 926 901 310 293 262 197
SnaBI TAC'GTA
Cuts at: 493 5104 5598 493
Size: 4611 494 2702
Fragments arranged by size:
4611 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7807
SphI G_CATG'C
Cuts at: 1132 5165 1132
Size: 4033 3774
SspI AAT'ATT
Cuts at: 5 52 5731 5
Size: 47 5679 2081
Fragments arranged by size:
5679 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7807
StyI C'CwwG_G
Cuts at: 513 1065 2934 3036 4461 4656 4776 4848 4854
Size: 552 1869 102 1425 195 120 72 6
Cuts at: 4854 4911 4965 5091 5246 513
Size: 57 54 126 155 3074
Fragments arranged by size:
3074 1869 1425 552 195 155 126 120
102 72 57 54 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2931 4648 5834 5851 6004 6189 6634
Size: 1255 1351 1717 1186 17 153 185 445
Cuts at: 6634 325
Size: 1498
Fragments arranged by size:
1717 1498 1351 1255 1186 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6053 7765 96
Size: 274 80 33 51 1443 4076 1712 138
Fragments arranged by size:
4076 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7767 7799 7767
Size: 32 7775
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7807
XbaI T'CTAG_A
Cuts at: 5143 5143
Size: 7807
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3043 3118 3151 3043
Size: 75 33 7699
Fragments arranged by size:
7699 75 33
XhoI C'TCGA_G
Cuts at: 1943 2546 1943
Size: 603 7204
XmaI C'CCGG_G
Cuts at: 1870 5132 1870
Size: 3262 4545
XmnI GAAnn'nnTTC
Cuts at: 3111 5936 3111
Size: 2825 4982
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2181 2826 5615 276
Size: 53 83 186 936 647 645 2789 2468
Fragments arranged by size:
2789 2468 936 647 645 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PspOMI PsrI RsrII SacII SanDI SapI
SexAI SfiI SrfI SwaI