Restriction map for
pFN21ASDA0844
(Circular) (Six-base) MAPSORT of: pFN21ASDA0844.seq Check: 7081 from: 1 to: 5950
pFN21ASDA0844 5950 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3760 278
Size: 53 83 186 936 2224 2468
Fragments arranged by size:
2468 2224 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3293 1937
Size: 1356 4594
Acc65I G'GTAC_C
Cuts at: 3271 3271
Size: 5950
AclI AA'CG_TT
Cuts at: 1563 4077 4450 1563
Size: 2514 373 3063
Fragments arranged by size:
3063 2514 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4013 5397 5665 1632
Size: 2381 1384 268 1917
Fragments arranged by size:
2381 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5753
AflIII A'CryG_T
Cuts at: 1203 2500 4849 5826 1203
Size: 1297 2349 977 1327
Fragments arranged by size:
2349 1327 1297 977
AgeI A'CCGG_T
Cuts at: 4781 4781
Size: 5950
AhdI GACnn_n'nnGTC
Cuts at: 4679 4679
Size: 5950
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5950
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5950
AlwNI CAG_nnn'CTG
Cuts at: 3693 5265 3693
Size: 1572 4378
ApaI G_GGCC'C
Cuts at: 2158 2158
Size: 5950
ApaLI G'TGCA_C
Cuts at: 4007 5163 4007
Size: 1156 4794
ApoI r'AATT_y
Cuts at: 1493 2513 3259 3485 3637 5746 1493
Size: 1020 746 226 152 2109 1697
Fragments arranged by size:
2109 1697 1020 746 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4504 160
Size: 872 3472 1606
Fragments arranged by size:
3472 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5950
AvaI C'yCGr_G
Cuts at: 1870 1943 2107 3275 1870
Size: 73 164 1168 4545
Fragments arranged by size:
4545 1168 164 73
BamHI G'GATC_C
Cuts at: 3280 3647 3280
Size: 367 5583
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2994 3271 4726 618
Size: 325 217 627 1207 277 1455 1842
Fragments arranged by size:
1842 1455 1207 627 325 277 217
BanII G_rGCy'C
Cuts at: 1391 2158 3168 3269 1391
Size: 767 1010 101 4072
Fragments arranged by size:
4072 1010 767 101
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4979
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4141 4175 1903
Size: 34 2204 34 3678
Fragments arranged by size:
3678 2204 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3842 5052 3842
Size: 1210 4740
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5950
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3186 4561 136
Size: 107 122 71 631 605 1514 1375 1525
Fragments arranged by size:
1525 1514 1375 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5945 5945
Size: 5950
BlpI GC'TnA_GC
Cuts at: 3367 3367
Size: 5950
Bme1580I G_kGCm'C
Cuts at: 1790 2158 2943 2997 4011 5167 1790
Size: 368 785 54 1014 1156 2573
Fragments arranged by size:
2573 1156 1014 785 368 54
BmgBI CAC'GTC
Cuts at: 1318 2692 1318
Size: 1374 4576
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3681 4639 442
Size: 416 53 83 399 24 2264 958 1753
Fragments arranged by size:
2264 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5950
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2183 2819 4610 1239
Size: 944 636 1791 2579
Fragments arranged by size:
2579 1791 944 636
Bpu10I CC'TnA_GC
Cuts at: 2984 2984
Size: 5950
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2170 2909 3011 3115 4075 4940 5238 2170
Size: 739 102 104 960 865 298 2882
Fragments arranged by size:
2882 960 865 739 298 104 102
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2112 3129 4613 882
Size: 617 613 1017 1484 2219
Fragments arranged by size:
2219 1484 1017 617 613
BsaAI yAC'GTr
Cuts at: 493 1204 2944 3247 3741 493
Size: 711 1740 303 494 2702
Fragments arranged by size:
2702 1740 711 494 303
BsaBI GATnn'nnATC
Cuts at: 5944 5944
Size: 5950
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3757 4139 4774 275
Size: 53 83 186 936 2224 382 635 1451
Fragments arranged by size:
2224 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4383 4781 5055 5202 5613 4383
Size: 398 274 147 411 4720
Fragments arranged by size:
4720 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5725
Fragments arranged by size:
5725 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2756 2759 2950 1186
Size: 1570 3 191 4186
Fragments arranged by size:
4186 1570 191 3
BseYI C'CCAG_C
Cuts at: 5153 5649 5153
Size: 496 5454
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2420 2648 1163
Size: 573 684 228 4465
Fragments arranged by size:
4465 684 573 228
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2453 3400 4161 5189 1540
Size: 275 186 452 947 761 1028 2301
Fragments arranged by size:
2301 1028 947 761 452 275 186
BsiHKAI G_wGCw'C
Cuts at: 3168 4011 4096 5167 3168
Size: 843 85 1071 3951
Fragments arranged by size:
3951 1071 843 85
BsmI GAATG_Cn'
Cuts at: 3001 3461 3554 3001
Size: 460 93 5397
Fragments arranged by size:
5397 460 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2653 2653
Size: 5950
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2158 2943 2997 3168 3269 4011 4096
Size: 399 368 785 54 171 101 742 85
Cuts at: 4096 5167 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 785 742 399 368 171 101
85 54
BspEI T'CCGG_A
Cuts at: 5613 5613
Size: 5950
BspHI T'CATG_A
Cuts at: 3839 5569 3839
Size: 1730 4220
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3305 844
Size: 2461 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2668 3837 1394
Size: 429 845 1169 3507
Fragments arranged by size:
3507 1169 845 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4445 4619 65
Size: 1183 3197 174 1396
Fragments arranged by size:
3197 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4594 4781 1700
Size: 110 2784 187 2869
Fragments arranged by size:
2869 2784 187 110
BsrGI T'GTAC_A
Cuts at: 96 2325 96
Size: 2229 3721
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5950
BssSI C'ACGA_G
Cuts at: 4010 5022 4010
Size: 1012 4938
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5950
BstBI TT'CG_AA
Cuts at: 2007 4754 2007
Size: 2747 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5950
BstXI CCAn_nnnn'nTGG
Cuts at: 2581 2581
Size: 5950
BstYI r'GATC_y
Cuts at: 1972 3280 3647 4032 4049 5501 5587 5599 5945
Size: 1308 367 385 17 1452 86 12 346
Cuts at: 5945 1972
Size: 1977
Fragments arranged by size:
1977 1452 1308 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2415 2697 513
Size: 552 1350 282 3766
Fragments arranged by size:
3766 1350 552 282
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5211
Fragments arranged by size:
5211 683 56
BtsI GCAGTG_nn'
Cuts at: 2290 2409 3466 4260 4280 2290
Size: 119 1057 794 20 3960
Fragments arranged by size:
3960 1057 794 119 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5950
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5915
DraI TTT'AAA
Cuts at: 3254 3254
Size: 5950
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4957 809
Size: 507 3641 1802
Fragments arranged by size:
3641 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2694 3178 3397
Size: 54 1425 50 275 18 864 484 219
Cuts at: 3397 4286 5915 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 864 484 275 219 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3397 1537
Size: 275 1585 4090
Fragments arranged by size:
4090 1585 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2549 3880 1353
Size: 1196 1331 3423
Fragments arranged by size:
3423 1331 1196
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2891 4537 4911 5057 387
Size: 1064 138 1302 1646 374 146 1280
Fragments arranged by size:
1646 1302 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3166 3166
Size: 5950
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2183 2819 4013 4610 5397 5665 1239
Size: 393 551 636 1194 597 787 268 1524
Fragments arranged by size:
1524 1194 787 636 597 551 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2965 3065 2965
Size: 100 5850
EcoO109I rG'GnC_Cy
Cuts at: 2154 2456 2154
Size: 302 5648
EcoRI G'AATT_C
Cuts at: 3259 3637 3259
Size: 378 5572
EcoRV GAT'ATC
Cuts at: 2054 2054
Size: 5950
FspI TGC'GCA
Cuts at: 4456 4456
Size: 5950
HaeII r_GCGC'y
Cuts at: 1678 5097 1678
Size: 3419 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2143 2175 2945 2977 3014
Size: 32 135 32 571 32 770 32 37
Cuts at: 3014 3046 4591 4623 4665 4697 1373
Size: 32 1545 32 42 32 2626
Fragments arranged by size:
2626 1545 770 571 135 42 37 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2320 3294 3540 669
Size: 988 281 382 974 246 3079
Fragments arranged by size:
3079 988 974 382 281 246
HindIII A'AGCT_T
Cuts at: 748 2137 2484 748
Size: 1389 347 4214
Fragments arranged by size:
4214 1389 347
HpaI GTT'AAC
Cuts at: 2320 3540 2320
Size: 1220 4730
KpnI G_GTAC'C
Cuts at: 3275 3275
Size: 5950
MfeI C'AATT_G
Cuts at: 3549 3549
Size: 5950
MluI A'CGCG_T
Cuts at: 5826 5826
Size: 5950
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2586 5064 5248 1305
Size: 1281 2478 184 2007
Fragments arranged by size:
2478 2007 1281 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4128
Fragments arranged by size:
4128 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2414 3908 4267 4426 518
Size: 648 1248 1494 359 159 2042
Fragments arranged by size:
2042 1494 1248 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2632 2804 2863 2937 3366 4043 5191 5436
Size: 1026 172 59 74 429 677 1148 245
Cuts at: 5436 1606
Size: 2120
Fragments arranged by size:
2120 1148 1026 677 429 245 172 74
59
NcoI C'CATG_G
Cuts at: 513 1065 2415 513
Size: 552 1350 4048
Fragments arranged by size:
4048 1350 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5950
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5950
NotI GC'GGCC_GC
Cuts at: 3397 3397
Size: 5950
NspI r_CATG'y
Cuts at: 1132 1229 2504 3094 3308 4853 1132
Size: 97 1275 590 214 1545 2229
Fragments arranged by size:
2229 1545 1275 590 214 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5950
PciI A'CATG_T
Cuts at: 2500 4849 2500
Size: 2349 3601
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2791 1108
Size: 1683 4267
PfoI T'CCnGG_A
Cuts at: 2458 2790 2835 2458
Size: 332 45 5573
Fragments arranged by size:
5573 332 45
PmeI GTTT'AAAC
Cuts at: 3254 3254
Size: 5950
PmlI CAC'GTG
Cuts at: 2944 2944
Size: 5950
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4019 4051 5532 5564 4019
Size: 32 1481 32 4405
Fragments arranged by size:
4405 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2456 2456
Size: 5950
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5950
PsiI TTA'TAA
Cuts at: 3520 3520
Size: 5950
PspOMI G'GGCC_C
Cuts at: 2154 2154
Size: 5950
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3227 3259 3227
Size: 32 5918
PstI C_TGCA'G
Cuts at: 830 3146 3302 830
Size: 2316 156 3478
Fragments arranged by size:
3478 2316 156
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5950
PvuII CAG'CTG
Cuts at: 2632 2804 2632
Size: 172 5778
SacI G_AGCT'C
Cuts at: 3168 3168
Size: 5950
SalI G'TCGA_C
Cuts at: 1936 3292 1936
Size: 1356 4594
SbfI CC_TGCA'GG
Cuts at: 3302 3302
Size: 5950
ScaI AGT'ACT
Cuts at: 2894 4198 5910 2894
Size: 1304 1712 2934
Fragments arranged by size:
2934 1712 1304
SfcI C'TryA_G
Cuts at: 826 1045 3142 3298 4433 5114 5305 826
Size: 219 2097 156 1135 681 191 1471
Fragments arranged by size:
2097 1471 1135 681 219 191 156
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5950
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5950
SmaI CCC'GGG
Cuts at: 1872 3277 1872
Size: 1405 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2149 2888 3026 3130 4054 4955
Size: 197 926 206 739 138 104 924 901
Cuts at: 4955 5217 820
Size: 262 1553
Fragments arranged by size:
1553 926 924 901 739 262 206 197
138 104
SnaBI TAC'GTA
Cuts at: 493 3247 3741 493
Size: 2754 494 2702
Fragments arranged by size:
2754 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5950
SphI G_CATG'C
Cuts at: 1132 3094 3308 1132
Size: 1962 214 3774
Fragments arranged by size:
3774 1962 214
SspI AAT'ATT
Cuts at: 5 52 3874 5
Size: 47 3822 2081
Fragments arranged by size:
3822 2081 47
StuI AGG'CCT
Cuts at: 1517 2386 2912 1517
Size: 869 526 4555
Fragments arranged by size:
4555 869 526
StyI C'CwwG_G
Cuts at: 513 1065 2349 2415 3072 3102 3389 513
Size: 552 1284 66 657 30 287 3074
Fragments arranged by size:
3074 1284 657 552 287 66 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3057 3977 3994 4147 4332 4777 325
Size: 1255 1477 920 17 153 185 445 1498
Fragments arranged by size:
1498 1477 1255 920 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2325 2713 2892
Size: 274 80 33 51 1443 348 388 179
Cuts at: 2892 4196 5908 96
Size: 1304 1712 138
Fragments arranged by size:
1712 1443 1304 388 348 274 179 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5910 5942 5910
Size: 32 5918
Tth111I GACn'n_nGTC
Cuts at: 1316 2240 2449 1316
Size: 924 209 4817
Fragments arranged by size:
4817 924 209
XbaI T'CTAG_A
Cuts at: 3286 3286
Size: 5950
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2912 2912
Size: 5950
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5950
XmaI C'CCGG_G
Cuts at: 1870 3275 1870
Size: 1405 4545
XmnI GAAnn'nnTTC
Cuts at: 4079 4079
Size: 5950
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3758 276
Size: 53 83 186 936 2224 2468
Fragments arranged by size:
2468 2224 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstZ17I Bsu36I DraIII FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI