Restriction map for
pFN21ASDA0843
(Circular) (Six-base) MAPSORT of: pFN21ASDA0843.seq Check: 7959 from: 1 to: 6778
pFN21ASDA0843 6778 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2117 2117
Size: 6778
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4588 278
Size: 53 83 186 936 3052 2468
Fragments arranged by size:
3052 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3383 3942 4121 1937
Size: 1446 559 179 4594
Fragments arranged by size:
4594 1446 559 179
Acc65I G'GTAC_C
Cuts at: 4099 4099
Size: 6778
AclI AA'CG_TT
Cuts at: 1563 4905 5278 1563
Size: 3342 373 3063
Fragments arranged by size:
3342 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2374 3623 4070 4841 6225 6493 1632
Size: 742 1249 447 771 1384 268 1917
Fragments arranged by size:
1917 1384 1249 771 742 447 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6581
AflIII A'CryG_T
Cuts at: 1203 3027 5677 6654 1203
Size: 1824 2650 977 1327
Fragments arranged by size:
2650 1824 1327 977
AgeI A'CCGG_T
Cuts at: 5609 5609
Size: 6778
AhdI GACnn_n'nnGTC
Cuts at: 5507 5507
Size: 6778
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6778
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6778
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3067 3099 3067
Size: 32 6746
AlwNI CAG_nnn'CTG
Cuts at: 4521 6093 4521
Size: 1572 5206
ApaI G_GGCC'C
Cuts at: 3231 3231
Size: 6778
ApaLI G'TGCA_C
Cuts at: 2132 4835 5991 2132
Size: 2703 1156 2919
Fragments arranged by size:
2919 2703 1156
ApoI r'AATT_y
Cuts at: 1493 4087 4313 4465 6574 1493
Size: 2594 226 152 2109 1697
Fragments arranged by size:
2594 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5332 160
Size: 872 4300 1606
Fragments arranged by size:
4300 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6778
AvaI C'yCGr_G
Cuts at: 1870 1943 2817 3142 3230 3513 4103 1870
Size: 73 874 325 88 283 590 4545
Fragments arranged by size:
4545 874 590 325 283 88 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2659 2692 2659
Size: 33 6745
BamHI G'GATC_C
Cuts at: 2876 4108 4475 2876
Size: 1232 367 5179
Fragments arranged by size:
5179 1232 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2258 2793 4099 5554 618
Size: 325 217 627 471 535 1306 1455 1842
Fragments arranged by size:
1842 1455 1306 627 535 471 325 217
BanII G_rGCy'C
Cuts at: 1391 3231 3248 3632 3665 4097 1391
Size: 1840 17 384 33 432 4072
Fragments arranged by size:
4072 1840 432 384 33 17
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3417 928
Size: 971 1518 4289
Fragments arranged by size:
4289 1518 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2612 2646 2889 2923 4969 5003 1903
Size: 34 675 34 243 34 2046 34 3678
Fragments arranged by size:
3678 2046 675 243 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4670 5880 4670
Size: 1210 5568
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6778
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3177 5389 136
Size: 107 122 71 631 605 1505 2212 1525
Fragments arranged by size:
2212 1525 1505 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3879 6773 3879
Size: 2894 3884
BlpI GC'TnA_GC
Cuts at: 4195 4195
Size: 6778
Bme1580I G_kGCm'C
Cuts at: 1790 2136 3231 3513 4839 5995 1790
Size: 346 1095 282 1326 1156 2573
Fragments arranged by size:
2573 1326 1156 1095 346 282
BmgBI CAC'GTC
Cuts at: 1318 2539 1318
Size: 1221 5557
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2188 2419 2629
Size: 416 53 83 399 24 771 231 210
Cuts at: 2629 3868 4509 5467 442
Size: 1239 641 958 1753
Fragments arranged by size:
1753 1239 958 771 641 416 399 231
210 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6778
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3065 3121 3348 3555 5438 1239
Size: 1826 56 227 207 1883 2579
Fragments arranged by size:
2579 1883 1826 227 207 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4903 5768 6066 4903
Size: 865 298 5615
Fragments arranged by size:
5615 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5441 882
Size: 617 3942 2219
Fragments arranged by size:
3942 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4075 4569 493
Size: 711 2871 494 2702
Fragments arranged by size:
2871 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 6772 6772
Size: 6778
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4585 4967 5602 275
Size: 53 83 186 936 3052 382 635 1451
Fragments arranged by size:
3052 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3345 5211 5609 5883 6030 6441 3345
Size: 1866 398 274 147 411 3682
Fragments arranged by size:
3682 1866 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2367 2397 3067 3097 3140
Size: 30 165 30 964 30 670 30 43
Cuts at: 3140 3170 3464 3494 1178
Size: 30 294 30 4462
Fragments arranged by size:
4462 964 670 294 165 43 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2504 3552 3557 1186
Size: 1318 1048 5 4407
Fragments arranged by size:
4407 1318 1048 5
BseYI C'CCAG_C
Cuts at: 3070 3322 3815 5981 6477 3070
Size: 252 493 2166 496 3371
Fragments arranged by size:
3371 2166 496 493 252
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2215 2365 2374 3671 3788 1163
Size: 573 479 150 9 1297 117 4153
Fragments arranged by size:
4153 1297 573 479 150 117 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3626 3731 4228 4989 6017 1540
Size: 275 186 1625 105 497 761 1028 2301
Fragments arranged by size:
2301 1625 1028 761 497 275 186 105
BsiHKAI G_wGCw'C
Cuts at: 2136 2423 3248 3632 3665 4839 4924 5995 2136
Size: 287 825 384 33 1174 85 1071 2919
Fragments arranged by size:
2919 1174 1071 825 384 287 85 33
BsmI GAATG_Cn'
Cuts at: 2034 4289 4382 2034
Size: 2255 93 4430
Fragments arranged by size:
4430 2255 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3121 3121
Size: 6778
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2136 2423 3231 3248 3513 3632 3665
Size: 399 346 287 808 17 265 119 33
Cuts at: 3665 4097 4839 4924 5995 1391
Size: 432 742 85 1071 2174
Fragments arranged by size:
2174 1071 808 742 432 399 346 287
265 119 85 33 17
BspEI T'CCGG_A
Cuts at: 6441 6441
Size: 6778
BspHI T'CATG_A
Cuts at: 4667 6397 4667
Size: 1730 5048
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2117 2570 4133 844
Size: 1273 453 1563 3489
Fragments arranged by size:
3489 1563 1273 453
BsrBI CCG'CTC
Cuts at: 1394 1823 3201 3222 4665 1394
Size: 429 1378 21 1443 3507
Fragments arranged by size:
3507 1443 1378 429 21
BsrDI GCAATG_nn'
Cuts at: 65 1248 5273 5447 65
Size: 1183 4025 174 1396
Fragments arranged by size:
4025 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3171 3591 4020 5422 5609 1700
Size: 110 1361 420 429 1402 187 2869
Fragments arranged by size:
2869 1402 1361 429 420 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6778
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6778
BssSI C'ACGA_G
Cuts at: 4838 5850 4838
Size: 1012 5766
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2352 794
Size: 1558 5220
BstBI TT'CG_AA
Cuts at: 2007 5582 2007
Size: 3575 3203
BstEII G'GTnAC_C
Cuts at: 1184 2383 2889 1184
Size: 1199 506 5073
Fragments arranged by size:
5073 1199 506
BstXI CCAn_nnnn'nTGG
Cuts at: 3995 3995
Size: 6778
BstYI r'GATC_y
Cuts at: 1972 2876 2928 3640 3879 4108 4475 4860 4877
Size: 904 52 712 239 229 367 385 17
Cuts at: 4877 6329 6415 6427 6773 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 904 712 385 367 346 239
229 86 52 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2063 2430 3493 513
Size: 552 998 367 1063 3798
Fragments arranged by size:
3798 1063 998 552 367
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6039
Fragments arranged by size:
6039 683 56
BtsI GCAGTG_nn'
Cuts at: 2537 4294 5088 5108 2537
Size: 1757 794 20 4207
Fragments arranged by size:
4207 1757 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6778
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2440 2475 529
Size: 35 1876 35 4832
Fragments arranged by size:
4832 1876 35 35
DraI TTT'AAA
Cuts at: 4082 4082
Size: 6778
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5785 809
Size: 507 4469 1802
Fragments arranged by size:
4469 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2433 2503 3238
Size: 54 1425 50 275 18 603 70 735
Cuts at: 3238 3589 4225 5114 6743 8
Size: 351 636 889 1629 43
Fragments arranged by size:
1629 1425 889 735 636 603 351 275
70 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4225 1537
Size: 275 2413 4090
Fragments arranged by size:
4090 2413 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3833 3972 4031 4708 1353
Size: 2480 139 59 677 3423
Fragments arranged by size:
3423 2480 677 139 59
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5365 5739 5885 387
Size: 1064 138 3776 374 146 1280
Fragments arranged by size:
3776 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3246 3630 3663 3246
Size: 384 33 6361
Fragments arranged by size:
6361 384 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2374 3065 3121 3348 3555 3623 4070
Size: 393 742 691 56 227 207 68 447
Cuts at: 4070 4841 5438 6225 6493 1239
Size: 771 597 787 268 1524
Fragments arranged by size:
1524 787 771 742 691 597 447 393
268 227 207 68 56
EcoNI CCTnn'n_nnAGG
Cuts at: 2722 3971 2722
Size: 1249 5529
EcoO109I rG'GnC_Cy
Cuts at: 3276 3498 3948 3276
Size: 222 450 6106
Fragments arranged by size:
6106 450 222
EcoRI G'AATT_C
Cuts at: 4087 4465 4087
Size: 378 6400
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2404 2404
Size: 6778
FspI TGC'GCA
Cuts at: 5284 5284
Size: 6778
HaeII r_GCGC'y
Cuts at: 1678 3964 5925 1678
Size: 2286 1961 2531
Fragments arranged by size:
2531 2286 1961
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3260 3292 5419 5451 5493
Size: 32 135 32 1688 32 2127 32 42
Cuts at: 5493 5525 1373
Size: 32 2626
Fragments arranged by size:
2626 2127 1688 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3856 4122 4368 669
Size: 988 281 1918 266 246 3079
Fragments arranged by size:
3079 1918 988 281 266 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6778
HpaI GTT'AAC
Cuts at: 4368 4368
Size: 6778
KpnI G_GTAC'C
Cuts at: 4103 4103
Size: 6778
MfeI C'AATT_G
Cuts at: 4377 4377
Size: 6778
MluI A'CGCG_T
Cuts at: 6654 6654
Size: 6778
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2646 3057 3311 5892 6076 1305
Size: 1341 411 254 2581 184 2007
Fragments arranged by size:
2581 2007 1341 411 254 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2435 10
Size: 54 1425 343 603 4353
Fragments arranged by size:
4353 1425 603 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4736 5095 5254 518
Size: 648 3570 359 159 2042
Fragments arranged by size:
3570 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2097 2265 2274 2394 2544 3326 3695 4194
Size: 491 168 9 120 150 782 369 499
Cuts at: 4194 4871 6019 6264 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 782 677 499 491 369 245
168 150 120 9
NaeI GCC'GGC
Cuts at: 3173 3593 3173
Size: 420 6358
NcoI C'CATG_G
Cuts at: 513 1065 2430 3493 513
Size: 552 1365 1063 3798
Fragments arranged by size:
3798 1365 1063 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6778
NgoMIV G'CCGG_C
Cuts at: 3171 3591 3171
Size: 420 6358
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6778
NotI GC'GGCC_GC
Cuts at: 4225 4225
Size: 6778
NspI r_CATG'y
Cuts at: 1132 1229 3031 3184 3568 3868 4136 5681 1132
Size: 97 1802 153 384 300 268 1545 2229
Fragments arranged by size:
2229 1802 1545 384 300 268 153 97
PasI CC'CwG_GG
Cuts at: 1348 2311 1348
Size: 963 5815
PciI A'CATG_T
Cuts at: 3027 5677 3027
Size: 2650 4128
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2431 1108
Size: 1323 5455
PmeI GTTT'AAAC
Cuts at: 4082 4082
Size: 6778
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3067 3099 4847 4879 6360 6392 3067
Size: 32 1748 32 1481 32 3453
Fragments arranged by size:
3453 1748 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3276 3498 3948 3276
Size: 222 450 6106
Fragments arranged by size:
6106 450 222
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6778
PsiI TTA'TAA
Cuts at: 4348 4348
Size: 6778
PspOMI G'GGCC_C
Cuts at: 3227 3227
Size: 6778
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2207 2239 2207
Size: 32 6746
PstI C_TGCA'G
Cuts at: 830 2567 4130 830
Size: 1737 1563 3478
Fragments arranged by size:
3478 1737 1563
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6778
PvuII CAG'CTG
Cuts at: 2274 2544 3326 3695 2274
Size: 270 782 369 5357
Fragments arranged by size:
5357 782 369 270
SacI G_AGCT'C
Cuts at: 3248 3632 3665 3248
Size: 384 33 6361
Fragments arranged by size:
6361 384 33
SalI G'TCGA_C
Cuts at: 1936 4120 1936
Size: 2184 4594
SanDI GG'GwC_CC
Cuts at: 3498 3498
Size: 6778
SbfI CC_TGCA'GG
Cuts at: 4130 4130
Size: 6778
ScaI AGT'ACT
Cuts at: 3482 5026 6738 3482
Size: 1544 1712 3522
Fragments arranged by size:
3522 1712 1544
SfcI C'TryA_G
Cuts at: 826 1045 2563 3166 3384 4126 5261 5942 6133
Size: 219 1518 603 218 742 1135 681 191
Cuts at: 6133 826
Size: 1471
Fragments arranged by size:
1518 1471 1135 742 681 603 219 218
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6778
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6778
SmaI CCC'GGG
Cuts at: 1872 2819 3144 4105 1872
Size: 947 325 961 4545
Fragments arranged by size:
4545 961 947 325
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4882 5783 6045 820
Size: 197 926 2939 901 262 1553
Fragments arranged by size:
2939 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4075 4569 493
Size: 3582 494 2702
Fragments arranged by size:
3582 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6778
SphI G_CATG'C
Cuts at: 1132 3568 3868 4136 1132
Size: 2436 300 268 3774
Fragments arranged by size:
3774 2436 300 268
SrfI GCCC'GGGC
Cuts at: 2819 2819
Size: 6778
SspI AAT'ATT
Cuts at: 5 52 4702 5
Size: 47 4650 2081
Fragments arranged by size:
4650 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6778
StyI C'CwwG_G
Cuts at: 513 1065 2430 2961 3493 3933 4217 513
Size: 552 1365 531 532 440 284 3074
Fragments arranged by size:
3074 1365 552 532 531 440 284
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2249 2679 2727 2908 3717 3829 4805
Size: 1255 669 430 48 181 809 112 976
Cuts at: 4805 4822 4975 5160 5605 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 976 809 669 445 430 185
181 153 112 48 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2223 3432 3480
Size: 274 80 33 51 1443 246 1209 48
Cuts at: 3480 3873 5024 6736 96
Size: 393 1151 1712 138
Fragments arranged by size:
1712 1443 1209 1151 393 274 246 138
80 51 48 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2020 2052 2367 2399 2516 2548 6738 6770 2020
Size: 32 315 32 117 32 4190 32 2028
Fragments arranged by size:
4190 2028 315 117 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6778
XbaI T'CTAG_A
Cuts at: 4114 4114
Size: 6778
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2252 2636 3584 2252
Size: 384 948 5446
Fragments arranged by size:
5446 948 384
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6778
XmaI C'CCGG_G
Cuts at: 1870 2817 3142 4103 1870
Size: 947 325 961 4545
Fragments arranged by size:
4545 961 947 325
XmnI GAAnn'nnTTC
Cuts at: 2363 4907 2363
Size: 2544 4234
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4586 276
Size: 53 83 186 936 3052 2468
Fragments arranged by size:
3052 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SacI SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I
BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI
NarI NruI NsiI PacI PfoI PmlI PspXI RsrII
SacII SapI SexAI SfiI SfoI SwaI