Restriction map for
pFN21ASDA0842
(Circular) (Six-base) MAPSORT of: pFN21ASDA0842.seq Check: 1135 from: 1 to: 7786
pFN21ASDA0842 7786 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3807 3807
Size: 7786
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4461 5596 278
Size: 53 83 186 936 2925 1135 2468
Fragments arranged by size:
2925 2468 1135 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3786 3927 5129 1937
Size: 1849 141 1202 4594
Fragments arranged by size:
4594 1849 1202 141
Acc65I G'GTAC_C
Cuts at: 5107 5107
Size: 7786
AclI AA'CG_TT
Cuts at: 1563 5913 6286 1563
Size: 4350 373 3063
Fragments arranged by size:
4350 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5849 7233 7501 1632
Size: 4217 1384 268 1917
Fragments arranged by size:
4217 1917 1384 268
AfeI AGC'GCT
Cuts at: 3185 3236 3715 3185
Size: 51 479 7256
Fragments arranged by size:
7256 479 51
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7589
AflIII A'CryG_T
Cuts at: 1203 3819 6685 7662 1203
Size: 2616 2866 977 1327
Fragments arranged by size:
2866 2616 1327 977
AgeI A'CCGG_T
Cuts at: 6617 6617
Size: 7786
AhdI GACnn_n'nnGTC
Cuts at: 2596 4003 6515 2596
Size: 1407 2512 3867
Fragments arranged by size:
3867 2512 1407
AleI CACnn'nnGTG
Cuts at: 1166 2882 1166
Size: 1716 6070
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7786
AlwNI CAG_nnn'CTG
Cuts at: 2155 2545 2858 2923 3250 3297 3601 3653 3898
Size: 390 313 65 327 47 304 52 245
Cuts at: 3898 4066 4677 5025 5046 5529 7101 2155
Size: 168 611 348 21 483 1572 2840
Fragments arranged by size:
2840 1572 611 483 390 348 327 313
304 245 168 65 52 47 21
ApaI G_GGCC'C
Cuts at: 4301 4301
Size: 7786
ApaLI G'TGCA_C
Cuts at: 2885 4042 4265 5843 6999 2885
Size: 1157 223 1578 1156 3672
Fragments arranged by size:
3672 1578 1157 1156 223
ApoI r'AATT_y
Cuts at: 1493 4202 4991 5095 5321 5473 7582 1493
Size: 2709 789 104 226 152 2109 1697
Fragments arranged by size:
2709 2109 1697 789 226 152 104
AseI AT'TA_AT
Cuts at: 160 1032 6340 160
Size: 872 5308 1606
Fragments arranged by size:
5308 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7786
AvaI C'yCGr_G
Cuts at: 1870 1943 2951 3347 4325 5064 5111 1870
Size: 73 1008 396 978 739 47 4545
Fragments arranged by size:
4545 1008 978 739 396 73 47
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2525 2558 2525
Size: 33 7753
BamHI G'GATC_C
Cuts at: 2049 4428 5116 5483 2049
Size: 2379 688 367 4352
Fragments arranged by size:
4352 2379 688 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4331 4370 5107 6562 618
Size: 325 217 627 2544 39 737 1455 1842
Fragments arranged by size:
2544 1842 1455 737 627 325 217 39
BanII G_rGCy'C
Cuts at: 1391 2816 2952 3318 3752 4301 5105 1391
Size: 1425 136 366 434 549 804 4072
Fragments arranged by size:
4072 1425 804 549 434 366 136
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2565 4406 928
Size: 971 666 1841 4308
Fragments arranged by size:
4308 1841 971 666
BbvCI CC'TCA_GC
Cuts at: 3717 3717
Size: 7786
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2219 2253 2932 2966 3244 3278 5977
Size: 34 282 34 679 34 278 34 2699
Cuts at: 5977 6011 1903
Size: 34 3678
Fragments arranged by size:
3678 2699 679 282 278 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3571 5678 6888 3571
Size: 2107 1210 4469
Fragments arranged by size:
4469 2107 1210
BclI T'GATC_A
Cuts at: 1548 4128 1548
Size: 2580 5206
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3087 4604 6397
Size: 107 122 71 631 605 1415 1517 1793
Cuts at: 6397 136
Size: 1525
Fragments arranged by size:
1793 1525 1517 1415 631 605 122 107
71
BglII A'GATC_T
Cuts at: 7781 7781
Size: 7786
BlpI GC'TnA_GC
Cuts at: 3312 4418 5203 3312
Size: 1106 785 5895
Fragments arranged by size:
5895 1106 785
Bme1580I G_kGCm'C
Cuts at: 1790 2889 3876 4046 4269 4301 4334 4373 4766
Size: 1099 987 170 223 32 33 39 393
Cuts at: 4766 5847 7003 1790
Size: 1081 1156 2573
Fragments arranged by size:
2573 1156 1099 1081 987 393 223 170
39 33 32
BmgBI CAC'GTC
Cuts at: 1318 4404 1318
Size: 3086 4700
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2224 2737 4279
Size: 416 53 83 399 24 807 513 1542
Cuts at: 4279 5517 6475 442
Size: 1238 958 1753
Fragments arranged by size:
1753 1542 1238 958 807 513 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7786
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2080 3609 2080
Size: 1529 6257
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2075 2657 3623 4595 6446 1239
Size: 836 582 966 972 1851 2579
Fragments arranged by size:
2579 1851 972 966 836 582
Bpu10I CC'TnA_GC
Cuts at: 3251 3594 3717 4877 3251
Size: 343 123 1160 6160
Fragments arranged by size:
6160 1160 343 123
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3665 5026 5911 6776 7074 3665
Size: 1361 885 865 298 4377
Fragments arranged by size:
4377 1361 885 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2733 3681 5061 6449 882
Size: 617 1234 948 1380 1388 2219
Fragments arranged by size:
2219 1388 1380 1234 948 617
BsaAI yAC'GTr
Cuts at: 493 1204 2278 3820 3904 4722 5083 5577 493
Size: 711 1074 1542 84 818 361 494 2702
Fragments arranged by size:
2702 1542 1074 818 711 494 361 84
BsaBI GATnn'nnATC
Cuts at: 7780 7780
Size: 7786
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3560 4458 5593 5975
Size: 53 83 186 936 2027 898 1135 382
Cuts at: 5975 6610 275
Size: 635 1451
Fragments arranged by size:
2027 1451 1135 936 898 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2046 2127 3858 3936 6219 6617 6891 7038 7449
Size: 81 1731 78 2283 398 274 147 411
Cuts at: 7449 2046
Size: 2383
Fragments arranged by size:
2383 2283 1731 411 398 274 147 81
78
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2318 2348 3328 3358 5026
Size: 30 165 30 915 30 980 30 1668
Cuts at: 5026 5056 1178
Size: 30 3908
Fragments arranged by size:
3908 1668 980 915 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2194 2857 2858 2924 3029 3158 3555 3558
Size: 1008 663 1 66 105 129 397 3
Cuts at: 3558 3561 4844 1186
Size: 3 1283 4128
Fragments arranged by size:
4128 1283 1008 663 397 129 105 66
3 3 1
BseYI C'CCAG_C
Cuts at: 2570 2717 2820 3290 3609 3695 4157 4670 4774
Size: 147 103 470 319 86 462 513 104
Cuts at: 4774 6989 7485 2570
Size: 2215 496 2871
Fragments arranged by size:
2871 2215 513 496 470 462 319 147
104 103 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2926 4340 4867 4896 1163
Size: 573 1190 1414 527 29 4053
Fragments arranged by size:
4053 1414 1190 573 527 29
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4564 5056 5236 5997 7025 1540
Size: 275 186 2563 492 180 761 1028 2301
Fragments arranged by size:
2563 2301 1028 761 492 275 186 180
BsiHKAI G_wGCw'C
Cuts at: 2166 2223 2889 2952 3305 3869 4046 4269 4419
Size: 57 666 63 353 564 177 223 150
Cuts at: 4419 5847 5932 7003 2166
Size: 1428 85 1071 2949
Fragments arranged by size:
2949 1428 1071 666 564 353 223 177
150 85 63 57
BsmI GAATG_Cn'
Cuts at: 2407 5297 5390 2407
Size: 2890 93 4803
Fragments arranged by size:
4803 2890 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4798 4798
Size: 7786
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2166 2223 2816 2889 2952 3305 3318
Size: 399 376 57 593 73 63 353 13
Cuts at: 3318 3752 3869 3876 4046 4269 4301 4334 4373
Size: 434 117 7 170 223 32 33 39
Cuts at: 4373 4419 4766 5105 5847 5932 7003 1391
Size: 46 347 339 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 593 434 399 376 353
347 339 223 170 117 85 73 63
57 46 39 33 32 13 7
BspEI T'CCGG_A
Cuts at: 2127 3858 3936 7449 2127
Size: 1731 78 3513 2464
Fragments arranged by size:
3513 2464 1731 78
BspHI T'CATG_A
Cuts at: 3810 5675 7405 3810
Size: 1865 1730 4191
Fragments arranged by size:
4191 1865 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2358 2553 3807 3897 3939 5141 844
Size: 1514 195 1254 90 42 1202 3489
Fragments arranged by size:
3489 1514 1254 1202 195 90 42
BsrBI CCG'CTC
Cuts at: 1394 1823 2769 4755 5673 1394
Size: 429 946 1986 918 3507
Fragments arranged by size:
3507 1986 946 918 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6281 6455 65
Size: 1183 5033 174 1396
Fragments arranged by size:
5033 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2848 3338 4563 6430 6617 1700
Size: 110 1038 490 1225 1867 187 2869
Fragments arranged by size:
2869 1867 1225 1038 490 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7786
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7786
BssSI C'ACGA_G
Cuts at: 2222 3583 5846 6858 2222
Size: 1361 2263 1012 3150
Fragments arranged by size:
3150 2263 1361 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3300 4066 4686 794
Size: 2506 766 620 3894
Fragments arranged by size:
3894 2506 766 620
BstBI TT'CG_AA
Cuts at: 2007 6590 2007
Size: 4583 3203
BstEII G'GTnAC_C
Cuts at: 1184 2965 1184
Size: 1781 6005
BstXI CCAn_nnnn'nTGG
Cuts at: 2290 2635 4207 2290
Size: 345 1572 5869
Fragments arranged by size:
5869 1572 345
BstYI r'GATC_y
Cuts at: 1972 2049 2115 2482 4428 5116 5483 5868 5885
Size: 77 66 367 1946 688 367 385 17
Cuts at: 5885 7337 7423 7435 7781 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1946 1452 688 385 367 367 346
86 77 66 17 12
Bsu36I CC'TnA_GG
Cuts at: 3513 3513
Size: 7786
BtgI C'CryG_G
Cuts at: 513 1065 2022 2581 3168 3228 4179 4524 5050
Size: 552 957 559 587 60 951 345 526
Cuts at: 5050 513
Size: 3249
Fragments arranged by size:
3249 957 951 587 559 552 526 345
60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7047
Fragments arranged by size:
7047 683 56
BtsI GCAGTG_nn'
Cuts at: 2709 4312 4502 5302 6096 6116 2709
Size: 1603 190 800 794 20 4379
Fragments arranged by size:
4379 1603 800 794 190 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7786
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7751
DraI TTT'AAA
Cuts at: 5090 5090
Size: 7786
DraIII CAC_nnn'GTG
Cuts at: 3300 3300
Size: 7786
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2449 3923 6793 809
Size: 507 1133 1474 2870 1802
Fragments arranged by size:
2870 1802 1474 1133 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2280 3460 3484
Size: 54 1425 50 275 18 450 1180 24
Cuts at: 3484 3523 4197 4605 5053 5233 6122 7751 8
Size: 39 674 408 448 180 889 1629 43
Fragments arranged by size:
1629 1425 1180 889 674 450 448 408
275 180 54 50 43 39 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 5053 5233 1537
Size: 275 3241 180 4090
Fragments arranged by size:
4090 3241 275 180
EarI CTCTTCn'nnn_
Cuts at: 1353 2441 3528 3831 3966 5716 1353
Size: 1088 1087 303 135 1750 3423
Fragments arranged by size:
3423 1750 1088 1087 303 135
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6373 6747 6893 387
Size: 1064 138 4784 374 146 1280
Fragments arranged by size:
4784 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2950 2950
Size: 7786
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2657 3623 4595 5849 6446 7233
Size: 393 443 582 966 972 1254 597 787
Cuts at: 7233 7501 1239
Size: 268 1524
Fragments arranged by size:
1524 1254 972 966 787 597 582 443
393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2806 3254 4385 2806
Size: 448 1131 6207
Fragments arranged by size:
6207 1131 448
EcoO109I rG'GnC_Cy
Cuts at: 2143 2920 3632 3657 4276 4298 2143
Size: 777 712 25 619 22 5631
Fragments arranged by size:
5631 777 712 619 25 22
EcoRI G'AATT_C
Cuts at: 5095 5473 5095
Size: 378 7408
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2582 4423 2582
Size: 1841 5945
FspI TGC'GCA
Cuts at: 6292 6292
Size: 7786
HaeII r_GCGC'y
Cuts at: 1678 2827 3187 3238 3717 4091 4791 5025 6933
Size: 1149 360 51 479 374 700 234 1908
Cuts at: 6933 1678
Size: 2531
Fragments arranged by size:
2531 1908 1149 700 479 374 360 234
51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2189 2221 2235 2267 2582
Size: 32 135 32 617 32 14 32 315
Cuts at: 2582 2614 2846 2878 3328 3360 3989 4021 4606
Size: 32 232 32 450 32 629 32 585
Cuts at: 4606 4638 5026 5058 6427 6459 6501 6533 1373
Size: 32 388 32 1369 32 42 32 2626
Fragments arranged by size:
2626 1369 629 617 585 450 388 315
232 135 42 32 32 32 32 32
32 32 32 32 32 32 32 14
HincII GTy'rAC
Cuts at: 669 1657 1938 5130 5376 669
Size: 988 281 3192 246 3079
Fragments arranged by size:
3192 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7786
HpaI GTT'AAC
Cuts at: 5376 5376
Size: 7786
KpnI G_GTAC'C
Cuts at: 5111 5111
Size: 7786
MfeI C'AATT_G
Cuts at: 5385 5385
Size: 7786
MluI A'CGCG_T
Cuts at: 7662 7662
Size: 7786
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2805 4584 5004 6900 7084 1305
Size: 1500 1779 420 1896 184 2007
Fragments arranged by size:
2007 1896 1779 1500 420 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2282 3486 3525 4199 10
Size: 54 1425 343 450 1204 39 674 3597
Fragments arranged by size:
3597 1425 1204 674 450 343 54 39
MslI CAynn'nnrTG
Cuts at: 518 1166 2882 3227 5744 6103 6262 518
Size: 648 1716 345 2517 359 159 2042
Fragments arranged by size:
2517 2042 1716 648 359 345 159
MspA1I CmG'CkG
Cuts at: 1606 3283 3493 3613 3844 3895 5032 5052 5202
Size: 1677 210 120 231 51 1137 20 150
Cuts at: 5202 5879 7027 7272 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1677 1148 1137 677 245 231 210
150 120 51 20
NaeI GCC'GGC
Cuts at: 2850 2850
Size: 7786
NcoI C'CATG_G
Cuts at: 513 1065 2022 3228 4179 513
Size: 552 957 1206 951 4120
Fragments arranged by size:
4120 1206 957 951 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7786
NgoMIV G'CCGG_C
Cuts at: 2848 2848
Size: 7786
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7786
NotI GC'GGCC_GC
Cuts at: 5233 5233
Size: 7786
NspI r_CATG'y
Cuts at: 1132 1229 2106 2521 3500 4354 5144 6689 1132
Size: 97 877 415 979 854 790 1545 2229
Fragments arranged by size:
2229 1545 979 877 854 790 415 97
PasI CC'CwG_GG
Cuts at: 1348 4294 4657 4863 1348
Size: 2946 363 206 4271
Fragments arranged by size:
4271 2946 363 206
PciI A'CATG_T
Cuts at: 6685 6685
Size: 7786
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2923 3101 3297 3653 1108
Size: 1815 178 196 356 5241
Fragments arranged by size:
5241 1815 356 196 178
PfoI T'CCnGG_A
Cuts at: 2052 2361 4968 2052
Size: 309 2607 4870
Fragments arranged by size:
4870 2607 309
PmeI GTTT'AAAC
Cuts at: 5090 5090
Size: 7786
PmlI CAC'GTG
Cuts at: 2278 3820 2278
Size: 1542 6244
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3830 3862 5855 5887 7368 7400 3830
Size: 32 1993 32 1481 32 4216
Fragments arranged by size:
4216 1993 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2143 3657 4276 2143
Size: 1514 619 5653
Fragments arranged by size:
5653 1514 619
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7786
PsiI TTA'TAA
Cuts at: 5356 5356
Size: 7786
PspOMI G'GGCC_C
Cuts at: 4297 4297
Size: 7786
PspXI vC'TCGA_Gb
Cuts at: 2951 2951
Size: 7786
PstI C_TGCA'G
Cuts at: 830 2274 2417 3044 3478 3894 5138 830
Size: 1444 143 627 434 416 1244 3478
Fragments arranged by size:
3478 1444 1244 627 434 416 143
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7786
PvuII CAG'CTG
Cuts at: 3283 3613 3844 3895 3283
Size: 330 231 51 7174
Fragments arranged by size:
7174 330 231 51
RsrII CG'GwC_CG
Cuts at: 3336 4447 3336
Size: 1111 6675
SacI G_AGCT'C
Cuts at: 2952 2952
Size: 7786
SacII CC_GC'GG
Cuts at: 5053 5053
Size: 7786
SalI G'TCGA_C
Cuts at: 1936 5128 1936
Size: 3192 4594
SanDI GG'GwC_CC
Cuts at: 3657 3657
Size: 7786
SapI GCTCTTCn'nnn_
Cuts at: 2441 3831 2441
Size: 1390 6396
SbfI CC_TGCA'GG
Cuts at: 3478 5138 3478
Size: 1660 6126
ScaI AGT'ACT
Cuts at: 2390 4817 6034 7746 2390
Size: 2427 1217 1712 2430
Fragments arranged by size:
2430 2427 1712 1217
SexAI A'CCwGG_T
Cuts at: 3296 3963 3296
Size: 667 7119
SfcI C'TryA_G
Cuts at: 826 1045 2147 2270 2413 3040 3474 3890 5134
Size: 219 1102 123 143 627 434 416 1244
Cuts at: 5134 6269 6950 7141 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1244 1135 1102 681 627 434 416
219 191 143 123
SfiI GGCCn_nnn'nGGCC
Cuts at: 4604 4604
Size: 7786
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7786
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7786
SmaI CCC'GGG
Cuts at: 1872 3349 5113 1872
Size: 1477 1764 4545
Fragments arranged by size:
4545 1764 1477
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2951 3680 5005 5890 6791 7053
Size: 197 926 1008 729 1325 885 901 262
Cuts at: 7053 820
Size: 1553
Fragments arranged by size:
1553 1325 1008 926 901 885 729 262
197
SnaBI TAC'GTA
Cuts at: 493 5083 5577 493
Size: 4590 494 2702
Fragments arranged by size:
4590 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7786
SphI G_CATG'C
Cuts at: 1132 4354 5144 1132
Size: 3222 790 3774
Fragments arranged by size:
3774 3222 790
SspI AAT'ATT
Cuts at: 5 52 5710 5
Size: 47 5658 2081
Fragments arranged by size:
5658 2081 47
StuI AGG'CCT
Cuts at: 1517 2801 1517
Size: 1284 6502
StyI C'CwwG_G
Cuts at: 513 1065 2022 2337 2445 3100 3228 4179 5225
Size: 552 957 315 108 655 128 951 1046
Cuts at: 5225 513
Size: 3074
Fragments arranged by size:
3074 1046 957 951 655 552 315 128
108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2206 2566 2834 4914 5813 5830 5983
Size: 1255 626 360 268 2080 899 17 153
Cuts at: 5983 6168 6613 325
Size: 185 445 1498
Fragments arranged by size:
2080 1498 1255 899 626 445 360 268
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2388 4815 6032
Size: 274 80 33 51 1443 411 2427 1217
Cuts at: 6032 7744 96
Size: 1712 138
Fragments arranged by size:
2427 1712 1443 1217 411 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7746 7778 7746
Size: 32 7754
Tth111I GACn'n_nGTC
Cuts at: 1316 2141 2753 2860 1316
Size: 825 612 107 6242
Fragments arranged by size:
6242 825 612 107
XbaI T'CTAG_A
Cuts at: 5122 5122
Size: 7786
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3235 4186 4942 3235
Size: 951 756 6079
Fragments arranged by size:
6079 951 756
XhoI C'TCGA_G
Cuts at: 1943 2951 1943
Size: 1008 6778
XmaI C'CCGG_G
Cuts at: 1870 3347 5111 1870
Size: 1477 1764 4545
Fragments arranged by size:
4545 1764 1477
XmnI GAAnn'nnTTC
Cuts at: 2834 4991 5915 2834
Size: 2157 924 4705
Fragments arranged by size:
4705 2157 924
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4459 5594 276
Size: 53 83 186 936 2925 1135 2468
Fragments arranged by size:
2925 2468 1135 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BbeI BfrBI BsiWI BstZ17I EcoRV
FseI FspAI KasI NarI NruI NsiI PacI PsrI
SfoI SrfI SwaI