Restriction map for
pFN21ASDA0839
(Circular) (Six-base) MAPSORT of: pFN21ASDA0839.seq Check: 719 from: 1 to: 8908
pFN21ASDA0839 8908 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2103 2926 2103
Size: 823 8085
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6718 278
Size: 53 83 186 936 5182 2468
Fragments arranged by size:
5182 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2794 6251 1937
Size: 857 3457 4594
Fragments arranged by size:
4594 3457 857
Acc65I G'GTAC_C
Cuts at: 3294 6229 3294
Size: 2935 5973
AclI AA'CG_TT
Cuts at: 1563 3630 3829 7035 7408 1563
Size: 2067 199 3206 373 3063
Fragments arranged by size:
3206 3063 2067 373 199
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2223 2490 4703 4858 4902 5327 5561 6093
Size: 591 267 2213 155 44 425 234 532
Cuts at: 6093 6971 8355 8623 1632
Size: 878 1384 268 1917
Fragments arranged by size:
2213 1917 1384 878 591 532 425 268
267 234 155 44
AflII C'TTAA_G
Cuts at: 820 1017 4061 820
Size: 197 3044 5667
Fragments arranged by size:
5667 3044 197
AflIII A'CryG_T
Cuts at: 1203 5164 7807 8784 1203
Size: 3961 2643 977 1327
Fragments arranged by size:
3961 2643 1327 977
AgeI A'CCGG_T
Cuts at: 7739 7739
Size: 8908
AhdI GACnn_n'nnGTC
Cuts at: 7637 7637
Size: 8908
AleI CACnn'nnGTG
Cuts at: 1166 6074 1166
Size: 4908 4000
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4578 5086 1184
Size: 3394 508 5006
Fragments arranged by size:
5006 3394 508
AlwNI CAG_nnn'CTG
Cuts at: 3608 4726 5935 6651 8223 3608
Size: 1118 1209 716 1572 4293
Fragments arranged by size:
4293 1572 1209 1118 716
ApaLI G'TGCA_C
Cuts at: 6965 8121 6965
Size: 1156 7752
ApoI r'AATT_y
Cuts at: 1493 3382 3441 3979 4598 5644 6217 6443 6595
Size: 1889 59 538 619 1046 573 226 152
Cuts at: 6595 8704 1493
Size: 2109 1697
Fragments arranged by size:
2109 1889 1697 1046 619 573 538 226
152 59
AseI AT'TA_AT
Cuts at: 160 1032 3764 7462 160
Size: 872 2732 3698 1606
Fragments arranged by size:
3698 2732 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8908
AvaI C'yCGr_G
Cuts at: 1870 1943 2073 2298 6233 1870
Size: 73 130 225 3935 4545
Fragments arranged by size:
4545 3935 225 130 73
BamHI G'GATC_C
Cuts at: 5638 6238 6605 5638
Size: 600 367 7941
Fragments arranged by size:
7941 600 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2117 3294 5386 6229 7684
Size: 325 217 627 330 1177 2092 843 1455
Cuts at: 7684 618
Size: 1842
Fragments arranged by size:
2092 1842 1455 1177 843 627 330 325
217
BanII G_rGCy'C
Cuts at: 1391 5049 6227 1391
Size: 3658 1178 4072
Fragments arranged by size:
4072 3658 1178
BbeI G_GCGC'C
Cuts at: 2121 2121
Size: 8908
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2873 4394 5549 928
Size: 971 974 1521 1155 4287
Fragments arranged by size:
4287 1521 1155 974 971
BbvCI CC'TCA_GC
Cuts at: 2111 4320 2111
Size: 2209 6699
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7099 7133 1903
Size: 34 5162 34 3678
Fragments arranged by size:
5162 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2444 3528 5392 6800 8010 2444
Size: 1084 1864 1408 1210 3342
Fragments arranged by size:
3342 1864 1408 1210 1084
BclI T'GATC_A
Cuts at: 1548 2818 2857 1548
Size: 1270 39 7599
Fragments arranged by size:
7599 1270 39
BfrBI ATG'CAT
Cuts at: 5588 5588
Size: 8908
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7519 136
Size: 107 122 71 631 605 5847 1525
Fragments arranged by size:
5847 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3585 5346 8903 3585
Size: 1761 3557 3590
Fragments arranged by size:
3590 3557 1761
BlpI GC'TnA_GC
Cuts at: 4779 6325 4779
Size: 1546 7362
Bme1580I G_kGCm'C
Cuts at: 1790 6969 8125 1790
Size: 5179 1156 2573
Fragments arranged by size:
5179 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8908
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2130 2161 2475
Size: 416 53 83 399 24 713 31 314
Cuts at: 2475 2567 6639 7597 442
Size: 92 4072 958 1753
Fragments arranged by size:
4072 1753 958 713 416 399 314 92
83 53 31 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8908
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5512 5553 5512
Size: 41 8867
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2048 2401 4052 4721 7568 1239
Size: 809 353 1651 669 2847 2579
Fragments arranged by size:
2847 2579 1651 809 669 353
Bpu10I CC'TnA_GC
Cuts at: 2111 4320 4424 2111
Size: 2209 104 6595
Fragments arranged by size:
6595 2209 104
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3917 4982 5063 7033 7898 8196 3917
Size: 1065 81 1970 865 298 4629
Fragments arranged by size:
4629 1970 1065 865 298 81
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4445 7571 882
Size: 617 2946 3126 2219
Fragments arranged by size:
3126 2946 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 6205 6699 493
Size: 711 5001 494 2702
Fragments arranged by size:
5001 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 8902 8902
Size: 8908
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2118 2470 3175 6715
Size: 53 83 186 936 585 352 705 3540
Cuts at: 6715 7097 7732 275
Size: 382 635 1451
Fragments arranged by size:
3540 1451 936 705 635 585 382 352
186 83 53
BsaWI w'CCGG_w
Cuts at: 7341 7739 8013 8160 8571 7341
Size: 398 274 147 411 7678
Fragments arranged by size:
7678 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3709 3739 5737 5767 1178
Size: 30 165 30 2306 30 1998 30 4319
Fragments arranged by size:
4319 2306 1998 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2117 2470 1186
Size: 931 353 7624
Fragments arranged by size:
7624 931 353
BseYI C'CCAG_C
Cuts at: 5073 8111 8607 5073
Size: 3038 496 5374
Fragments arranged by size:
5374 3038 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4551 5709 1163
Size: 573 2815 1158 4362
Fragments arranged by size:
4362 2815 1158 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6358 7119 8147 1540
Size: 275 186 4357 761 1028 2301
Fragments arranged by size:
4357 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2428 2544 3461 4194 5049 5493 5510 5943 6969
Size: 116 917 733 855 444 17 433 1026
Cuts at: 6969 7054 8125 2428
Size: 85 1071 3211
Fragments arranged by size:
3211 1071 1026 917 855 733 444 433
116 85 17
BsmI GAATG_Cn'
Cuts at: 4555 6419 6512 4555
Size: 1864 93 6951
Fragments arranged by size:
6951 1864 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2428 2544 3461 4194 5049 5493 5510
Size: 399 638 116 917 733 855 444 17
Cuts at: 5510 5943 6227 6969 7054 8125 1391
Size: 433 284 742 85 1071 2174
Fragments arranged by size:
2174 1071 917 855 742 733 638 444
433 399 284 116 85 17
BspEI T'CCGG_A
Cuts at: 8571 8571
Size: 8908
BspHI T'CATG_A
Cuts at: 5526 5905 6797 8527 5526
Size: 379 892 1730 5907
Fragments arranged by size:
5907 1730 892 379
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2103 2492 2926 6263 844
Size: 1259 389 434 3337 3489
Fragments arranged by size:
3489 3337 1259 434 389
BsrBI CCG'CTC
Cuts at: 1394 1823 4532 6795 1394
Size: 429 2709 2263 3507
Fragments arranged by size:
3507 2709 2263 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4833 5065 5178 5313 6051 7403 7577
Size: 1183 3585 232 113 135 738 1352 174
Cuts at: 7577 65
Size: 1396
Fragments arranged by size:
3585 1396 1352 1183 738 232 174 135
113
BsrFI r'CCGG_y
Cuts at: 1700 1810 7552 7739 1700
Size: 110 5742 187 2869
Fragments arranged by size:
5742 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8908
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8908
BssSI C'ACGA_G
Cuts at: 6968 7980 6968
Size: 1012 7896
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2735 4492 794
Size: 1941 1757 5210
Fragments arranged by size:
5210 1941 1757
BstBI TT'CG_AA
Cuts at: 2007 7712 2007
Size: 5705 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8908
BstYI r'GATC_y
Cuts at: 1972 2106 3585 5101 5335 5346 5638 5728 6238
Size: 134 1479 1516 234 11 292 90 510
Cuts at: 6238 6605 6990 7007 8459 8545 8557 8903 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1516 1479 1452 510 385 367 346
292 234 134 90 86 17 12 11
BtgI C'CryG_G
Cuts at: 513 1065 2022 3178 4478 5421 513
Size: 552 957 1156 1300 943 4000
Fragments arranged by size:
4000 1300 1156 957 943 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8169
Fragments arranged by size:
8169 683 56
BtsI GCAGTG_nn'
Cuts at: 3502 4769 6424 7218 7238 3502
Size: 1267 1655 794 20 5172
Fragments arranged by size:
5172 1655 1267 794 20
ClaI AT'CG_AT
Cuts at: 1554 4446 1554
Size: 2892 6016
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2866 2901 5740 5775 529
Size: 35 2302 35 2839 35 3662
Fragments arranged by size:
3662 2839 2302 35 35 35
DraI TTT'AAA
Cuts at: 3543 3882 4864 6212 3543
Size: 339 982 1348 6239
Fragments arranged by size:
6239 1348 982 339
DraIII CAC_nnn'GTG
Cuts at: 3653 3691 6027 3653
Size: 38 2336 6534
Fragments arranged by size:
6534 2336 38
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5915 7915 809
Size: 507 4599 2000 1802
Fragments arranged by size:
4599 2000 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4440 5777 6355
Size: 54 1425 50 275 18 2610 1337 578
Cuts at: 6355 7244 8873 8
Size: 889 1629 43
Fragments arranged by size:
2610 1629 1425 1337 889 578 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6355 1537
Size: 275 4543 4090
Fragments arranged by size:
4543 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2634 2709 3656 4209 5733 5880 6195 6838
Size: 1281 75 947 553 1524 147 315 643
Cuts at: 6838 1353
Size: 3423
Fragments arranged by size:
3423 1524 1281 947 643 553 315 147
75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7495 7869 8015 387
Size: 1064 138 5906 374 146 1280
Fragments arranged by size:
5906 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5047 5047
Size: 8908
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2048 2223 2401 2490 4052 4703 4721
Size: 393 416 175 178 89 1562 651 18
Cuts at: 4721 4858 4902 5327 5561 6093 6971 7568 8355
Size: 137 44 425 234 532 878 597 787
Cuts at: 8355 8623 1239
Size: 268 1524
Fragments arranged by size:
1562 1524 878 787 651 597 532 425
416 393 268 234 178 175 137 89
44 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2099 2125 3477 2099
Size: 26 1352 7530
Fragments arranged by size:
7530 1352 26
EcoO109I rG'GnC_Cy
Cuts at: 2059 2131 2569 3334 3470 4633 5917 6058 2059
Size: 72 438 765 136 1163 1284 141 4909
Fragments arranged by size:
4909 1284 1163 765 438 141 136 72
EcoRI G'AATT_C
Cuts at: 6217 6595 6217
Size: 378 8530
EcoRV GAT'ATC
Cuts at: 4575 4575
Size: 8908
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4076 5936 4076
Size: 1860 7048
FspI TGC'GCA
Cuts at: 4496 4545 7414 4496
Size: 49 2869 5990
Fragments arranged by size:
5990 2869 49
FspAI rTGC'GCAy
Cuts at: 4496 4496
Size: 8908
HaeII r_GCGC'y
Cuts at: 1678 2121 8055 1678
Size: 443 5934 2531
Fragments arranged by size:
5934 2531 443
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3590 3622 4451 4483 7549
Size: 32 135 32 2018 32 829 32 3066
Cuts at: 7549 7581 7623 7655 1373
Size: 32 42 32 2626
Fragments arranged by size:
3066 2626 2018 829 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4948 5872 6252 6498 669
Size: 988 281 3010 924 380 246 3079
Fragments arranged by size:
3079 3010 988 924 380 281 246
HindIII A'AGCT_T
Cuts at: 748 3801 748
Size: 3053 5855
HpaI GTT'AAC
Cuts at: 4948 6498 4948
Size: 1550 7358
KasI G'GCGC_C
Cuts at: 2117 2117
Size: 8908
KpnI G_GTAC'C
Cuts at: 3298 6233 3298
Size: 2935 5973
MfeI C'AATT_G
Cuts at: 6081 6507 6081
Size: 426 8482
MluI A'CGCG_T
Cuts at: 8784 8784
Size: 8908
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2297 3056 5230 8022 8206 1305
Size: 992 759 2174 2792 184 2007
Fragments arranged by size:
2792 2174 2007 992 759 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4442 5779 10
Size: 54 1425 343 2610 1337 3139
Fragments arranged by size:
3139 2610 1425 1337 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2492 2826 5306 6074 6866 7225 7384
Size: 648 1326 334 2480 768 792 359 159
Cuts at: 7384 518
Size: 2042
Fragments arranged by size:
2480 2042 1326 792 768 648 359 334
159
MspA1I CmG'CkG
Cuts at: 1606 2115 2663 4324 4699 5693 5932 6324 7001
Size: 509 548 1661 375 994 239 392 677
Cuts at: 7001 8149 8394 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1661 1148 994 677 548 509 392
375 245 239
NarI GG'CG_CC
Cuts at: 2118 2118
Size: 8908
NcoI C'CATG_G
Cuts at: 513 1065 2022 3178 4478 513
Size: 552 957 1156 1300 4943
Fragments arranged by size:
4943 1300 1156 957 552
NdeI CA'TA_TG
Cuts at: 387 3191 387
Size: 2804 6104
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8908
NotI GC'GGCC_GC
Cuts at: 6355 6355
Size: 8908
NsiI A_TGCA'T
Cuts at: 5590 5590
Size: 8908
NspI r_CATG'y
Cuts at: 1132 1229 3286 4550 5063 5168 6266 7811 1132
Size: 97 2057 1264 513 105 1098 1545 2229
Fragments arranged by size:
2229 2057 1545 1264 1098 513 105 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8908
PciI A'CATG_T
Cuts at: 5164 7807 5164
Size: 2643 6265
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2960 3066 3608 1108
Size: 1852 106 542 6408
Fragments arranged by size:
6408 1852 542 106
PfoI T'CCnGG_A
Cuts at: 2055 2055
Size: 8908
PmeI GTTT'AAAC
Cuts at: 6212 6212
Size: 8908
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6977 7009 8490 8522 6977
Size: 32 1481 32 7363
Fragments arranged by size:
7363 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2059 2131 2569 3334 3470 5917 6058 2059
Size: 72 438 765 136 2447 141 4909
Fragments arranged by size:
4909 2447 765 438 141 136 72
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8908
PsiI TTA'TAA
Cuts at: 2781 6478 2781
Size: 3697 5211
PstI C_TGCA'G
Cuts at: 830 2740 3509 4776 6260 830
Size: 1910 769 1267 1484 3478
Fragments arranged by size:
3478 1910 1484 1267 769
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8908
PvuII CAG'CTG
Cuts at: 2663 4324 4699 5693 5932 2663
Size: 1661 375 994 239 5639
Fragments arranged by size:
5639 1661 994 375 239
SacI G_AGCT'C
Cuts at: 5049 5049
Size: 8908
SalI G'TCGA_C
Cuts at: 1936 6250 1936
Size: 4314 4594
SanDI GG'GwC_CC
Cuts at: 2131 2131
Size: 8908
SbfI CC_TGCA'GG
Cuts at: 6260 6260
Size: 8908
ScaI AGT'ACT
Cuts at: 2577 4676 7156 8868 2577
Size: 2099 2480 1712 2617
Fragments arranged by size:
2617 2480 2099 1712
SfcI C'TryA_G
Cuts at: 826 1045 2736 2940 3327 3505 3920 4772 5897
Size: 219 1691 204 387 178 415 852 1125
Cuts at: 5897 6256 7391 8072 8263 826
Size: 359 1135 681 191 1471
Fragments arranged by size:
1691 1471 1135 1125 852 681 415 387
359 219 204 191 178
SfoI GGC'GCC
Cuts at: 2119 2119
Size: 8908
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8908
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8908
SmaI CCC'GGG
Cuts at: 1872 2075 6235 1872
Size: 203 4160 4545
Fragments arranged by size:
4545 4160 203
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2298 3896 4061 4961 5042 7012
Size: 197 926 355 1598 165 900 81 1970
Cuts at: 7012 7913 8175 820
Size: 901 262 1553
Fragments arranged by size:
1970 1598 1553 926 901 900 355 262
197 165 81
SnaBI TAC'GTA
Cuts at: 493 6205 6699 493
Size: 5712 494 2702
Fragments arranged by size:
5712 2702 494
SpeI A'CTAG_T
Cuts at: 152 6100 152
Size: 5948 2960
SphI G_CATG'C
Cuts at: 1132 5063 6266 1132
Size: 3931 1203 3774
Fragments arranged by size:
3931 3774 1203
SspI AAT'ATT
Cuts at: 5 52 4169 5154 6832 5
Size: 47 4117 985 1678 2081
Fragments arranged by size:
4117 2081 1678 985 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8908
StyI C'CwwG_G
Cuts at: 513 1065 2022 3178 3675 4478 4790 6347 513
Size: 552 957 1156 497 803 312 1557 3074
Fragments arranged by size:
3074 1557 1156 957 803 552 497 312
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4607 5764 5993 6034 6935 6952 7105
Size: 1255 3027 1157 229 41 901 17 153
Cuts at: 7105 7290 7735 325
Size: 185 445 1498
Fragments arranged by size:
3027 1498 1255 1157 901 445 229 185
153 41 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2575 4674 6029
Size: 274 80 33 51 1443 598 2099 1355
Cuts at: 6029 7154 8866 96
Size: 1125 1712 138
Fragments arranged by size:
2099 1712 1443 1355 1125 598 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2326 2358 2455 2487 5737 5769 8868 8900 2326
Size: 32 97 32 3250 32 3099 32 2334
Fragments arranged by size:
3250 3099 2334 97 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2845 1316
Size: 1529 7379
XbaI T'CTAG_A
Cuts at: 3170 6244 3170
Size: 3074 5834
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2284 3736 5446 2284
Size: 1452 1710 5746
Fragments arranged by size:
5746 1710 1452
XhoI C'TCGA_G
Cuts at: 1943 2298 1943
Size: 355 8553
XmaI C'CCGG_G
Cuts at: 1870 2073 6233 1870
Size: 203 4160 4545
Fragments arranged by size:
4545 4160 203
XmnI GAAnn'nnTTC
Cuts at: 3765 5201 7037 3765
Size: 1436 1836 5636
Fragments arranged by size:
5636 1836 1436
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6716 276
Size: 53 83 186 936 5182 2468
Fragments arranged by size:
5182 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BsiWI BsmBI
BstXI BstZ17I Bsu36I FseI NaeI NgoMIV NruI PacI
PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI
SfiI SrfI SwaI