Restriction map for
pFN21ASDA0833
(Circular) (Six-base) MAPSORT of: pFN21ASDA0833.seq Check: 2512 from: 1 to: 9748
pFN21ASDA0833 9748 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5526 5526
Size: 9748
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7558 278
Size: 53 83 186 936 6022 2468
Fragments arranged by size:
6022 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6014 7091 1937
Size: 4077 1077 4594
Fragments arranged by size:
4594 4077 1077
Acc65I G'GTAC_C
Cuts at: 7069 7069
Size: 9748
AclI AA'CG_TT
Cuts at: 1563 7875 8248 1563
Size: 6312 373 3063
Fragments arranged by size:
6312 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2513 3571 3763 3802 4312 5893 7811 9195
Size: 881 1058 192 39 510 1581 1918 1384
Cuts at: 9195 9463 1632
Size: 268 1917
Fragments arranged by size:
1918 1917 1581 1384 1058 881 510 268
192 39
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9551
AflIII A'CryG_T
Cuts at: 1203 2943 3399 3807 8647 9624 1203
Size: 1740 456 408 4840 977 1327
Fragments arranged by size:
4840 1740 1327 977 456 408
AgeI A'CCGG_T
Cuts at: 8579 8579
Size: 9748
AhdI GACnn_n'nnGTC
Cuts at: 3808 4924 8477 3808
Size: 1116 3553 5079
Fragments arranged by size:
5079 3553 1116
AleI CACnn'nnGTG
Cuts at: 1166 3161 3398 3947 1166
Size: 1995 237 549 6967
Fragments arranged by size:
6967 1995 549 237
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4259 4495 1184
Size: 3075 236 6437
Fragments arranged by size:
6437 3075 236
AlwNI CAG_nnn'CTG
Cuts at: 3993 4757 5942 6311 7491 9063 3993
Size: 764 1185 369 1180 1572 4678
Fragments arranged by size:
4678 1572 1185 1180 764 369
ApaI G_GGCC'C
Cuts at: 4459 4459
Size: 9748
ApaLI G'TGCA_C
Cuts at: 2552 7805 8961 2552
Size: 5253 1156 3339
Fragments arranged by size:
5253 3339 1156
ApoI r'AATT_y
Cuts at: 1493 5986 6268 6512 6761 6788 7057 7283 7435
Size: 4493 282 244 249 27 269 226 152
Cuts at: 7435 9544 1493
Size: 2109 1697
Fragments arranged by size:
4493 2109 1697 282 269 249 244 226
152 27
AseI AT'TA_AT
Cuts at: 160 1032 8302 160
Size: 872 7270 1606
Fragments arranged by size:
7270 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9748
AvaI C'yCGr_G
Cuts at: 1870 1943 3572 3862 4231 4884 5873 7073 1870
Size: 73 1629 290 369 653 989 1200 4545
Fragments arranged by size:
4545 1629 1200 989 653 369 290 73
AvrII C'CTAG_G
Cuts at: 4603 5380 4603
Size: 777 8971
BamHI G'GATC_C
Cuts at: 7078 7445 7078
Size: 367 9381
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2235 3941 4398 4598 7069
Size: 325 217 627 448 1706 457 200 2471
Cuts at: 7069 8524 618
Size: 1455 1842
Fragments arranged by size:
2471 1842 1706 1455 627 457 448 325
217 200
BanII G_rGCy'C
Cuts at: 1391 2664 2984 3095 3120 3332 4302 4459 4891
Size: 1273 320 111 25 212 970 157 432
Cuts at: 4891 5586 6443 7067 1391
Size: 695 857 624 4072
Fragments arranged by size:
4072 1273 970 857 695 624 432 320
212 157 111 25
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5695 928
Size: 971 3796 4981
Fragments arranged by size:
4981 3796 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2173 2207 7939 7973 1903
Size: 34 236 34 5732 34 3678
Fragments arranged by size:
5732 3678 236 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7640 8850 7640
Size: 1210 8538
BclI T'GATC_A
Cuts at: 1548 2140 4350 6574 1548
Size: 592 2210 2224 4722
Fragments arranged by size:
4722 2224 2210 592
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3828 4076 4589
Size: 107 122 71 631 605 2156 248 513
Cuts at: 4589 5174 6447 8359 136
Size: 585 1273 1912 1525
Fragments arranged by size:
2156 1912 1525 1273 631 605 585 513
248 122 107 71
BglII A'GATC_T
Cuts at: 4746 5995 9743 4746
Size: 1249 3748 4751
Fragments arranged by size:
4751 3748 1249
BlpI GC'TnA_GC
Cuts at: 4301 4520 5538 6558 7165 4301
Size: 219 1018 1020 607 6884
Fragments arranged by size:
6884 1020 1018 607 219
Bme1580I G_kGCm'C
Cuts at: 1790 2556 3944 4401 4459 4601 5380 5388 6279
Size: 766 1388 457 58 142 779 8 891
Cuts at: 6279 7809 8965 1790
Size: 1530 1156 2573
Fragments arranged by size:
2573 1530 1388 1156 891 779 766 457
142 58 8
BmgBI CAC'GTC
Cuts at: 1318 2944 3810 4564 1318
Size: 1626 866 754 6502
Fragments arranged by size:
6502 1626 866 754
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5112 5602 5883
Size: 416 53 83 399 24 3695 490 281
Cuts at: 5883 6369 7479 8437 442
Size: 486 1110 958 1753
Fragments arranged by size:
3695 1753 1110 958 490 486 416 399
281 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9748
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3070 3649 4594 5709 3070
Size: 579 945 1115 7109
Fragments arranged by size:
7109 1115 945 579
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2968 3703 3785 4991 5531 5619 5919 8408
Size: 1729 735 82 1206 540 88 300 2489
Cuts at: 8408 1239
Size: 2579
Fragments arranged by size:
2579 2489 1729 1206 735 540 300 88
82
Bpu10I CC'TnA_GC
Cuts at: 2728 2728
Size: 9748
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2426 4121 6457 7873 8738 9036 2426
Size: 1695 2336 1416 865 298 3138
Fragments arranged by size:
3138 2336 1695 1416 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3055 4094 4181 4582 8411 882
Size: 617 1556 1039 87 401 3829 2219
Fragments arranged by size:
3829 2219 1556 1039 617 401 87
BsaAI yAC'GTr
Cuts at: 493 1204 4006 7045 7539 493
Size: 711 2802 3039 494 2702
Fragments arranged by size:
3039 2802 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 4955 9742 4955
Size: 4787 4961
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3300 3882 4218 7555
Size: 53 83 186 936 1767 582 336 3337
Cuts at: 7555 7937 8572 275
Size: 382 635 1451
Fragments arranged by size:
3337 1767 1451 936 635 582 382 336
186 83 53
BsaWI w'CCGG_w
Cuts at: 2508 3167 6627 8181 8579 8853 9000 9411 2508
Size: 659 3460 1554 398 274 147 411 2845
Fragments arranged by size:
3460 2845 1554 659 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2450 2480 3656 3686 3962
Size: 30 165 30 1047 30 1176 30 276
Cuts at: 3962 3992 4325 4349 4355 4379 4671 4701 5333
Size: 30 333 24 6 24 292 30 632
Cuts at: 5333 5363 5643 5673 6476 6506 1178
Size: 30 280 30 803 30 4420
Fragments arranged by size:
4420 1176 1047 803 632 333 292 280
276 165 30 30 30 30 30 30
30 30 30 24 24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3043 3278 3658 3661 4692 4999 5349 1186
Size: 1857 235 380 3 1031 307 350 5585
Fragments arranged by size:
5585 1857 1031 380 350 307 235 3
BseYI C'CCAG_C
Cuts at: 2787 3752 4266 4271 4500 4798 4911 5010 5172
Size: 965 514 5 229 298 113 99 162
Cuts at: 5172 5542 5585 6142 8951 9447 2787
Size: 370 43 557 2809 496 3088
Fragments arranged by size:
3088 2809 965 557 514 496 370 298
229 162 113 99 43 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2774 2797 2951 3014 4265 5796 6183
Size: 573 1038 23 154 63 1251 1531 387
Cuts at: 6183 1163
Size: 4728
Fragments arranged by size:
4728 1531 1251 1038 573 387 154 63
23
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4584 7198 7959 8987 1540
Size: 275 186 2583 2614 761 1028 2301
Fragments arranged by size:
2614 2583 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2556 2664 2967 3027 3095 3332 4173 4302 5517
Size: 108 303 60 68 237 841 129 1215
Cuts at: 5517 6819 7809 7894 8965 2556
Size: 1302 990 85 1071 3339
Fragments arranged by size:
3339 1302 1215 1071 990 841 303 237
129 108 85 68 60
BsiWI C'GTAC_G
Cuts at: 3513 3513
Size: 9748
BsmI GAATG_Cn'
Cuts at: 6182 7259 7352 6182
Size: 1077 93 8578
Fragments arranged by size:
8578 1077 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4004 4004
Size: 9748
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2556 2664 2967 2984 3027 3095 3120
Size: 399 766 108 303 17 43 68 25
Cuts at: 3120 3332 3944 4173 4302 4401 4459 4601 4891
Size: 212 612 229 129 99 58 142 290
Cuts at: 4891 5380 5388 5517 5586 6279 6443 6819 7067
Size: 489 8 129 69 693 164 376 248
Cuts at: 7067 7809 7894 8965 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 766 742 693 612 489 399
376 303 290 248 229 212 164 142
129 129 108 99 85 69 68 58
43 25 17 8
BspEI T'CCGG_A
Cuts at: 6627 9411 6627
Size: 2784 6964
BspHI T'CATG_A
Cuts at: 6577 7637 9367 6577
Size: 1060 1730 6958
Fragments arranged by size:
6958 1730 1060
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2714 4185 5526 7103 844
Size: 1870 1471 1341 1577 3489
Fragments arranged by size:
3489 1870 1577 1471 1341
BsrBI CCG'CTC
Cuts at: 1394 1823 3580 4623 5031 5064 7635 1394
Size: 429 1757 1043 408 33 2571 3507
Fragments arranged by size:
3507 2571 1757 1043 429 408 33
BsrDI GCAATG_nn'
Cuts at: 65 1248 2719 8243 8417 65
Size: 1183 1471 5524 174 1396
Fragments arranged by size:
5524 1471 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2569 2816 3713 4211 8392 8579 1700
Size: 110 759 247 897 498 4181 187 2869
Fragments arranged by size:
4181 2869 897 759 498 247 187 110
BsrGI T'GTAC_A
Cuts at: 96 3249 5409 6889 7002 96
Size: 3153 2160 1480 113 2842
Fragments arranged by size:
3153 2842 2160 1480 113
BssHII G'CGCG_C
Cuts at: 1925 2029 1925
Size: 104 9644
BssSI C'ACGA_G
Cuts at: 2960 7808 8820 2960
Size: 4848 1012 3888
Fragments arranged by size:
4848 3888 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2595 4177 5090 794
Size: 1801 1582 913 5452
Fragments arranged by size:
5452 1801 1582 913
BstBI TT'CG_AA
Cuts at: 2007 8552 2007
Size: 6545 3203
BstEII G'GTnAC_C
Cuts at: 1184 3232 4090 4639 1184
Size: 2048 858 549 6293
Fragments arranged by size:
6293 2048 858 549
BstXI CCAn_nnnn'nTGG
Cuts at: 3067 4549 4811 4864 3067
Size: 1482 262 53 7951
Fragments arranged by size:
7951 1482 262 53
BstYI r'GATC_y
Cuts at: 1972 2760 4746 5963 5995 6387 7078 7445 7830
Size: 788 1986 1217 32 392 691 367 385
Cuts at: 7830 7847 9299 9385 9397 9743 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1986 1977 1452 1217 788 691 392 385
367 346 86 32 17 12
Bsu36I CC'TnA_GG
Cuts at: 6289 6289
Size: 9748
BtgI C'CryG_G
Cuts at: 513 1065 2689 2792 2897 3213 3360 3675 3957
Size: 552 1624 103 105 316 147 315 282
Cuts at: 3957 4095 4260 4368 4548 4703 5212 6086 6189
Size: 138 165 108 180 155 509 874 103
Cuts at: 6189 6279 6498 513
Size: 90 219 3763
Fragments arranged by size:
3763 1624 874 552 509 316 315 282
219 180 165 155 147 138 108 105
103 103 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 9009
Fragments arranged by size:
9009 683 56
BtsI GCAGTG_nn'
Cuts at: 5050 5728 5808 7264 8058 8078 5050
Size: 678 80 1456 794 20 6720
Fragments arranged by size:
6720 1456 794 678 80 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9748
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9713
DraI TTT'AAA
Cuts at: 7052 7052
Size: 9748
DraIII CAC_nnn'GTG
Cuts at: 5090 5090
Size: 9748
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8755 809
Size: 507 7439 1802
Fragments arranged by size:
7439 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2571 3012 3321
Size: 54 1425 50 275 18 741 441 309
Cuts at: 3321 3337 3439 3651 4581 4992 5235 6181 6342
Size: 16 102 212 930 411 243 946 161
Cuts at: 6342 7195 8084 9713 8
Size: 853 889 1629 43
Fragments arranged by size:
1629 1425 946 930 889 853 741 441
411 309 275 243 212 161 102 54
50 43 18 16
EagI C'GGCC_G
Cuts at: 1537 1812 4581 7195 1537
Size: 275 2769 2614 4090
Fragments arranged by size:
4090 2769 2614 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2060 2652 2990 3402 5574 5820 7678 1353
Size: 707 592 338 412 2172 246 1858 3423
Fragments arranged by size:
3423 2172 1858 707 592 412 338 246
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4130 6484 8335 8709 8855 387
Size: 1064 138 2541 2354 1851 374 146 1280
Fragments arranged by size:
2541 2354 1851 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2662 3093 3330 4300 2662
Size: 431 237 970 8110
Fragments arranged by size:
8110 970 431 237
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2513 2968 3571 3703 3763 3785 3802
Size: 393 881 455 603 132 60 22 17
Cuts at: 3802 4312 4991 5531 5619 5893 5919 7811 8408
Size: 510 679 540 88 274 26 1892 597
Cuts at: 8408 9195 9463 1239
Size: 787 268 1524
Fragments arranged by size:
1892 1524 881 787 679 603 597 540
510 455 393 274 268 132 88 60
26 22 17
EcoNI CCTnn'n_nnAGG
Cuts at: 3608 3608
Size: 9748
EcoO109I rG'GnC_Cy
Cuts at: 3310 4129 4456 4687 5007 5106 6147 3310
Size: 819 327 231 320 99 1041 6911
Fragments arranged by size:
6911 1041 819 327 320 231 99
EcoRI G'AATT_C
Cuts at: 5986 6512 7057 7435 5986
Size: 526 545 378 8299
Fragments arranged by size:
8299 545 526 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3367 5478 3367
Size: 2111 7637
FspI TGC'GCA
Cuts at: 8254 8254
Size: 9748
HaeII r_GCGC'y
Cuts at: 1678 2865 3213 3786 4212 4430 8895 1678
Size: 1187 348 573 426 218 4465 2531
Fragments arranged by size:
4465 2531 1187 573 426 348 218
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3365 3397 3794 3826 3962
Size: 32 135 32 1793 32 397 32 136
Cuts at: 3962 3994 4349 4381 4671 4703 4733 4765 4910
Size: 32 355 32 290 32 30 32 145
Cuts at: 4910 4942 5333 5365 6019 6051 6131 6163 8389
Size: 32 391 32 654 32 80 32 2226
Cuts at: 8389 8421 8463 8495 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2226 1793 654 397 391 355 290
145 136 135 80 42 32 32 32
32 32 32 32 32 32 32 32
32 32 32 30
HincII GTy'rAC
Cuts at: 669 1657 1938 5326 7092 7338 669
Size: 988 281 3388 1766 246 3079
Fragments arranged by size:
3388 3079 1766 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9748
HpaI GTT'AAC
Cuts at: 7338 7338
Size: 9748
KpnI G_GTAC'C
Cuts at: 7073 7073
Size: 9748
MfeI C'AATT_G
Cuts at: 7347 7347
Size: 9748
MluI A'CGCG_T
Cuts at: 9624 9624
Size: 9748
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2928 3576 3819 4314 4368 4881 4911 8862
Size: 1623 648 243 495 54 513 30 3951
Cuts at: 8862 9046 1305
Size: 184 2007
Fragments arranged by size:
3951 2007 1623 648 513 495 243 184
54 30
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3014 3339 3653 6344 10
Size: 54 1425 343 1182 325 314 2691 3414
Fragments arranged by size:
3414 2691 1425 1182 343 325 314 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3161 3398 3947 4367 6008 6203 7706
Size: 648 1995 237 549 420 1641 195 1503
Cuts at: 7706 8065 8224 518
Size: 359 159 2042
Fragments arranged by size:
2042 1995 1641 1503 648 549 420 359
237 195 159
MspA1I CmG'CkG
Cuts at: 1606 2241 2674 2749 2794 3114 3269 3677 3999
Size: 635 433 75 45 320 155 408 322
Cuts at: 3999 4405 4519 4731 5058 5214 5240 5542 6500
Size: 406 114 212 327 156 26 302 958
Cuts at: 6500 6969 7164 7841 8989 9234 1606
Size: 469 195 677 1148 245 2120
Fragments arranged by size:
2120 1148 958 677 635 469 433 408
406 327 322 320 302 245 212 195
156 155 114 75 45 26
NaeI GCC'GGC
Cuts at: 2571 3715 4213 2571
Size: 1144 498 8106
Fragments arranged by size:
8106 1144 498
NcoI C'CATG_G
Cuts at: 513 1065 2689 4095 4548 6189 6279 513
Size: 552 1624 1406 453 1641 90 3982
Fragments arranged by size:
3982 1641 1624 1406 552 453 90
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9748
NgoMIV G'CCGG_C
Cuts at: 2569 3713 4211 2569
Size: 1144 498 8106
Fragments arranged by size:
8106 1144 498
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9748
NotI GC'GGCC_GC
Cuts at: 7195 7195
Size: 9748
NspI r_CATG'y
Cuts at: 1132 1229 4561 4612 7106 8651 1132
Size: 97 3332 51 2494 1545 2229
Fragments arranged by size:
3332 2494 2229 1545 97 51
PasI CC'CwG_GG
Cuts at: 1348 4594 5245 1348
Size: 3246 651 5851
Fragments arranged by size:
5851 3246 651
PciI A'CATG_T
Cuts at: 8647 8647
Size: 9748
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2333 2455 6190 1108
Size: 1225 122 3735 4666
Fragments arranged by size:
4666 3735 1225 122
PfoI T'CCnGG_A
Cuts at: 4959 4959
Size: 9748
PmeI GTTT'AAAC
Cuts at: 7052 7052
Size: 9748
PmlI CAC'GTG
Cuts at: 4006 4006
Size: 9748
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2711 2743 7817 7849 9330 9362 2711
Size: 32 5074 32 1481 32 3097
Fragments arranged by size:
5074 3097 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4129 4687 5007 6147 4129
Size: 558 320 1140 7730
Fragments arranged by size:
7730 1140 558 320
PshAI GACnn'nnGTC
Cuts at: 1613 4812 1613
Size: 3199 6549
PsiI TTA'TAA
Cuts at: 6723 6843 7318 6723
Size: 120 475 9153
Fragments arranged by size:
9153 475 120
PspOMI G'GGCC_C
Cuts at: 4455 4455
Size: 9748
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5759 5791 5759
Size: 32 9716
PstI C_TGCA'G
Cuts at: 830 2711 3879 3998 4155 4182 4518 4617 5523
Size: 1881 1168 119 157 27 336 99 906
Cuts at: 5523 5947 7100 830
Size: 424 1153 3478
Fragments arranged by size:
3478 1881 1168 1153 906 424 336 157
119 99 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9748
PvuII CAG'CTG
Cuts at: 2674 2749 4405 4519 4731 5542 2674
Size: 75 1656 114 212 811 6880
Fragments arranged by size:
6880 1656 811 212 114 75
SacI G_AGCT'C
Cuts at: 2664 3095 3332 4302 2664
Size: 431 237 970 8110
Fragments arranged by size:
8110 970 431 237
SacII CC_GC'GG
Cuts at: 2795 3678 5215 6501 2795
Size: 883 1537 1286 6042
Fragments arranged by size:
6042 1537 1286 883
SalI G'TCGA_C
Cuts at: 1936 7090 1936
Size: 5154 4594
SanDI GG'GwC_CC
Cuts at: 5007 5007
Size: 9748
SapI GCTCTTCn'nnn_
Cuts at: 2060 2652 3402 5574 5820 2060
Size: 592 750 2172 246 5988
Fragments arranged by size:
5988 2172 750 592 246
SbfI CC_TGCA'GG
Cuts at: 3879 7100 3879
Size: 3221 6527
ScaI AGT'ACT
Cuts at: 5777 6830 7996 9708 5777
Size: 1053 1166 1712 5817
Fragments arranged by size:
5817 1712 1166 1053
SexAI A'CCwGG_T
Cuts at: 3063 3063
Size: 9748
SfcI C'TryA_G
Cuts at: 826 1045 2130 2707 3875 3994 4151 4178 4492
Size: 219 1085 577 1168 119 157 27 314
Cuts at: 4492 4514 4613 4726 5519 5716 5943 5973 6015
Size: 22 99 113 793 197 227 30 42
Cuts at: 6015 7096 8231 8912 9103 826
Size: 1081 1135 681 191 1471
Fragments arranged by size:
1471 1168 1135 1085 1081 793 681 577
314 227 219 197 191 157 119 113
99 42 30 27 22
SfiI GGCCn_nnn'nGGCC
Cuts at: 4589 4589
Size: 9748
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9748
SgrAI Cr'CCGG_yG
Cuts at: 1700 4211 1700
Size: 2511 7237
SmaI CCC'GGG
Cuts at: 1872 3574 4233 4886 7075 1872
Size: 1702 659 653 2189 4545
Fragments arranged by size:
4545 2189 1702 659 653
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2441 4136 6436 7852 8753 9015
Size: 197 926 498 1695 2300 1416 901 262
Cuts at: 9015 820
Size: 1553
Fragments arranged by size:
2300 1695 1553 1416 926 901 498 262
197
SnaBI TAC'GTA
Cuts at: 493 7045 7539 493
Size: 6552 494 2702
Fragments arranged by size:
6552 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9748
SphI G_CATG'C
Cuts at: 1132 4612 7106 1132
Size: 3480 2494 3774
Fragments arranged by size:
3774 3480 2494
SrfI GCCC'GGGC
Cuts at: 4886 4886
Size: 9748
SspI AAT'ATT
Cuts at: 5 52 7672 5
Size: 47 7620 2081
Fragments arranged by size:
7620 2081 47
StuI AGG'CCT
Cuts at: 1517 3528 3974 1517
Size: 2011 446 7291
Fragments arranged by size:
7291 2011 446
StyI C'CwwG_G
Cuts at: 513 1065 2689 2886 3609 4095 4548 4603 5109
Size: 552 1624 197 723 486 453 55 506
Cuts at: 5109 5380 5646 6189 6219 6279 7033 7187 513
Size: 271 266 543 30 60 754 154 3074
Fragments arranged by size:
3074 1624 754 723 552 543 506 486
453 271 266 197 154 60 55 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3101 3638 3929 4481 4658 6267 7775
Size: 1255 1521 537 291 552 177 1609 1508
Cuts at: 7775 7792 7945 8130 8575 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1609 1521 1508 1498 1255 552 537 445
291 185 177 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3249 4875 5354
Size: 274 80 33 51 1443 1272 1626 479
Cuts at: 5354 5409 5775 6019 6326 6828 6889 7002 7994
Size: 55 366 244 307 502 61 113 992
Cuts at: 7994 9706 96
Size: 1712 138
Fragments arranged by size:
1712 1626 1443 1272 992 502 479 366
307 274 244 138 113 80 61 55
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 9708 9740 9708
Size: 32 9716
Tth111I GACn'n_nGTC
Cuts at: 1316 2543 5005 1316
Size: 1227 2462 6059
Fragments arranged by size:
6059 2462 1227
XbaI T'CTAG_A
Cuts at: 7084 7084
Size: 9748
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3064 3760 3064
Size: 696 9052
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9748
XmaI C'CCGG_G
Cuts at: 1870 3572 4231 4884 7073 1870
Size: 1702 659 653 2189 4545
Fragments arranged by size:
4545 2189 1702 659 653
XmnI GAAnn'nnTTC
Cuts at: 7877 7877
Size: 9748
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7556 276
Size: 53 83 186 936 6022 2468
Fragments arranged by size:
6022 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BbvCI BfrBI BstZ17I
EcoRV FseI FspAI KasI NarI NruI NsiI PacI
PspXI RsrII SfoI SwaI