Restriction map for
pFN21ASDA0832
(Circular) (Six-base) MAPSORT of: pFN21ASDA0832.seq Check: 445 from: 1 to: 6103
pFN21ASDA0832 6103 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3913 278
Size: 53 83 186 936 2377 2468
Fragments arranged by size:
2468 2377 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2964 3396 3446 1937
Size: 1027 432 50 4594
Fragments arranged by size:
4594 1027 432 50
Acc65I G'GTAC_C
Cuts at: 2433 3424 2433
Size: 991 5112
AclI AA'CG_TT
Cuts at: 1563 4230 4603 1563
Size: 2667 373 3063
Fragments arranged by size:
3063 2667 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2190 3179 4166 5550 5818 1632
Size: 558 989 987 1384 268 1917
Fragments arranged by size:
1917 1384 989 987 558 268
AfeI AGC'GCT
Cuts at: 3216 3216
Size: 6103
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5906
AflIII A'CryG_T
Cuts at: 1203 5002 5979 1203
Size: 3799 977 1327
Fragments arranged by size:
3799 1327 977
AgeI A'CCGG_T
Cuts at: 2967 4934 2967
Size: 1967 4136
AhdI GACnn_n'nnGTC
Cuts at: 4832 4832
Size: 6103
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6103
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6103
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2212 2244 2212
Size: 32 6071
AlwNI CAG_nnn'CTG
Cuts at: 3846 5418 3846
Size: 1572 4531
ApaLI G'TGCA_C
Cuts at: 4160 5316 4160
Size: 1156 4947
ApoI r'AATT_y
Cuts at: 1493 2943 3116 3412 3638 3790 5899 1493
Size: 1450 173 296 226 152 2109 1697
Fragments arranged by size:
2109 1697 1450 296 226 173 152
AseI AT'TA_AT
Cuts at: 160 1032 4657 160
Size: 872 3625 1606
Fragments arranged by size:
3625 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6103
AvaI C'yCGr_G
Cuts at: 1870 1943 3428 1870
Size: 73 1485 4545
Fragments arranged by size:
4545 1485 73
BamHI G'GATC_C
Cuts at: 3433 3800 3433
Size: 367 5736
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2433 3424 4879 618
Size: 325 217 627 646 991 1455 1842
Fragments arranged by size:
1842 1455 991 646 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3422 1391
Size: 2031 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3030 3255 928
Size: 971 1131 225 3776
Fragments arranged by size:
3776 1131 971 225
BbvCI CC'TCA_GC
Cuts at: 2694 2694
Size: 6103
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4294 4328 1903
Size: 34 2357 34 3678
Fragments arranged by size:
3678 2357 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3995 5205 3995
Size: 1210 4893
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6103
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2199 2468 4714
Size: 107 122 71 631 605 527 269 2246
Cuts at: 4714 136
Size: 1525
Fragments arranged by size:
2246 1525 631 605 527 269 122 107
71
BglII A'GATC_T
Cuts at: 2766 6098 2766
Size: 3332 2771
BlpI GC'TnA_GC
Cuts at: 3520 3520
Size: 6103
Bme1580I G_kGCm'C
Cuts at: 1790 4164 5320 1790
Size: 2374 1156 2573
Fragments arranged by size:
2573 2374 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6103
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3834 4792 442
Size: 416 53 83 399 24 2417 958 1753
Fragments arranged by size:
2417 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6103
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2320 4763 1239
Size: 1081 2443 2579
Fragments arranged by size:
2579 2443 1081
Bpu10I CC'TnA_GC
Cuts at: 2694 2694
Size: 6103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4228 5093 5391 4228
Size: 865 298 4940
Fragments arranged by size:
4940 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2976 4766 882
Size: 617 1477 1790 2219
Fragments arranged by size:
2219 1790 1477 617
BsaAI yAC'GTr
Cuts at: 493 1204 2633 3400 3894 493
Size: 711 1429 767 494 2702
Fragments arranged by size:
2702 1429 767 711 494
BsaBI GATnn'nnATC
Cuts at: 6097 6097
Size: 6103
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2211 3910 4292 4927
Size: 53 83 186 936 678 1699 382 635
Cuts at: 4927 275
Size: 1451
Fragments arranged by size:
1699 1451 936 678 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2758 2967 4536 4934 5208 5355 5766 2758
Size: 209 1569 398 274 147 411 3095
Fragments arranged by size:
3095 1569 411 398 274 209 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2212 2242 3273 3303 1178
Size: 30 165 30 809 30 1031 30 3978
Fragments arranged by size:
3978 1031 809 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6103
BseYI C'CCAG_C
Cuts at: 2158 5306 5802 2158
Size: 3148 496 2459
Fragments arranged by size:
3148 2459 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2048 3338 1163
Size: 573 312 1290 3928
Fragments arranged by size:
3928 1290 573 312
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2842 3553 4314 5342 1540
Size: 275 186 841 711 761 1028 2301
Fragments arranged by size:
2301 1028 841 761 711 275 186
BsiHKAI G_wGCw'C
Cuts at: 4164 4249 5320 4164
Size: 85 1071 4947
Fragments arranged by size:
4947 1071 85
BsmI GAATG_Cn'
Cuts at: 2471 3614 3707 2471
Size: 1143 93 4867
Fragments arranged by size:
4867 1143 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3422 4164 4249 5320 1391
Size: 399 1632 742 85 1071 2174
Fragments arranged by size:
2174 1632 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5766 5766
Size: 6103
BspHI T'CATG_A
Cuts at: 2577 3992 5722 2577
Size: 1415 1730 2958
Fragments arranged by size:
2958 1730 1415
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3458 844
Size: 2614 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3990 1394
Size: 429 2167 3507
Fragments arranged by size:
3507 2167 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4598 4772 65
Size: 1183 3350 174 1396
Fragments arranged by size:
3350 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2967 4747 4934 1700
Size: 110 1157 1780 187 2869
Fragments arranged by size:
2869 1780 1157 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6103
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6103
BssSI C'ACGA_G
Cuts at: 4163 5175 4163
Size: 1012 5091
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6103
BstBI TT'CG_AA
Cuts at: 2007 4907 2007
Size: 2900 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6103
BstXI CCAn_nnnn'nTGG
Cuts at: 2195 2195
Size: 6103
BstYI r'GATC_y
Cuts at: 1972 2353 2766 3433 3800 4185 4202 5654 5740
Size: 381 413 667 367 385 17 1452 86
Cuts at: 5740 5752 6098 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 667 413 385 381 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 2965 2965
Size: 6103
Bsu36I CC'TnA_GG
Cuts at: 3293 3293
Size: 6103
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4594
Fragments arranged by size:
4594 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2407 487
Size: 683 56 1181 4183
Fragments arranged by size:
4183 1181 683 56
BtsI GCAGTG_nn'
Cuts at: 3281 3619 4413 4433 3281
Size: 338 794 20 4951
Fragments arranged by size:
4951 794 338 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6103
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6068
DraI TTT'AAA
Cuts at: 2589 3407 2589
Size: 818 5285
DraIII CAC_nnn'GTG
Cuts at: 2828 2828
Size: 6103
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2789 5110 809
Size: 507 1473 2321 1802
Fragments arranged by size:
2321 1802 1473 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2242 2835 3235
Size: 54 1425 50 275 18 412 593 400
Cuts at: 3235 3550 4439 6068 8
Size: 315 889 1629 43
Fragments arranged by size:
1629 1425 889 593 412 400 315 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3550 1537
Size: 275 1738 4090
Fragments arranged by size:
4090 1738 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2070 2209 4033 1353
Size: 717 139 1824 3423
Fragments arranged by size:
3423 1824 717 139
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2921 4690 5064 5210 387
Size: 1064 138 1332 1769 374 146 1280
Fragments arranged by size:
1769 1332 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2190 2320 3179 4166 4763 5550 5818
Size: 393 558 130 859 987 597 787 268
Cuts at: 5818 1239
Size: 1524
Fragments arranged by size:
1524 987 859 787 597 558 393 268
130
EcoRI G'AATT_C
Cuts at: 3412 3790 3412
Size: 378 5725
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2491 2491
Size: 6103
FspI TGC'GCA
Cuts at: 4609 4609
Size: 6103
HaeII r_GCGC'y
Cuts at: 1678 3218 5250 1678
Size: 1540 2032 2531
Fragments arranged by size:
2531 2032 1540
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2318 2350 4744 4776 4818
Size: 32 135 32 746 32 2394 32 42
Cuts at: 4818 4850 1373
Size: 32 2626
Fragments arranged by size:
2626 2394 746 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2182 3447 3693 669
Size: 988 281 244 1265 246 3079
Fragments arranged by size:
3079 1265 988 281 246 244
HindIII A'AGCT_T
Cuts at: 748 3191 748
Size: 2443 3660
HpaI GTT'AAC
Cuts at: 3693 3693
Size: 6103
KpnI G_GTAC'C
Cuts at: 2437 3428 2437
Size: 991 5112
MfeI C'AATT_G
Cuts at: 3702 3702
Size: 6103
MluI A'CGCG_T
Cuts at: 5979 5979
Size: 6103
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3363 5217 5401 1305
Size: 2058 1854 184 2007
Fragments arranged by size:
2058 2007 1854 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2244 3237 10
Size: 54 1425 343 412 993 2876
Fragments arranged by size:
2876 1425 993 412 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4061 4420 4579 518
Size: 648 2895 359 159 2042
Fragments arranged by size:
2895 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2124 2511 2698 3076 3519 4196 5344 5589
Size: 518 387 187 378 443 677 1148 245
Cuts at: 5589 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 518 443 387 378 245
187
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4594
Fragments arranged by size:
4594 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6103
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6103
NotI GC'GGCC_GC
Cuts at: 3550 3550
Size: 6103
NspI r_CATG'y
Cuts at: 1132 1229 2380 2602 3461 5006 1132
Size: 97 1151 222 859 1545 2229
Fragments arranged by size:
2229 1545 1151 859 222 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6103
PciI A'CATG_T
Cuts at: 5002 5002
Size: 6103
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3244 1108
Size: 2136 3967
PmeI GTTT'AAAC
Cuts at: 3407 3407
Size: 6103
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2212 2244 4172 4204 5685 5717 2212
Size: 32 1928 32 1481 32 2598
Fragments arranged by size:
2598 1928 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3255 1613
Size: 1642 4461
PsiI TTA'TAA
Cuts at: 3673 3673
Size: 6103
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2666 2698 2666
Size: 32 6071
PstI C_TGCA'G
Cuts at: 830 2090 2928 3075 3222 3455 830
Size: 1260 838 147 147 233 3478
Fragments arranged by size:
3478 1260 838 233 147 147
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6103
PvuII CAG'CTG
Cuts at: 2124 2511 2698 3076 2124
Size: 387 187 378 5151
Fragments arranged by size:
5151 387 378 187
SalI G'TCGA_C
Cuts at: 1936 3445 1936
Size: 1509 4594
SapI GCTCTTCn'nnn_
Cuts at: 2070 2209 2070
Size: 139 5964
SbfI CC_TGCA'GG
Cuts at: 3455 3455
Size: 6103
ScaI AGT'ACT
Cuts at: 4351 6063 4351
Size: 1712 4391
SfcI C'TryA_G
Cuts at: 826 1045 2086 2224 2924 3071 3135 3218 3451
Size: 219 1041 138 700 147 64 83 233
Cuts at: 3451 4586 5267 5458 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1041 700 681 233 219 191
147 138 83 64
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6103
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6103
SmaI CCC'GGG
Cuts at: 1872 3430 1872
Size: 1558 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4207 5108 5370 820
Size: 197 926 2264 901 262 1553
Fragments arranged by size:
2264 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3400 3894 493
Size: 2907 494 2702
Fragments arranged by size:
2907 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6103
SphI G_CATG'C
Cuts at: 1132 2602 3461 1132
Size: 1470 859 3774
Fragments arranged by size:
3774 1470 859
SspI AAT'ATT
Cuts at: 5 52 4027 5
Size: 47 3975 2081
Fragments arranged by size:
3975 2081 47
StuI AGG'CCT
Cuts at: 1517 3045 1517
Size: 1528 4575
StyI C'CwwG_G
Cuts at: 513 1065 2022 2953 3309 3390 3542 513
Size: 552 957 931 356 81 152 3074
Fragments arranged by size:
3074 957 931 552 356 152 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2856 4130 4147 4300 4485 4930 325
Size: 1255 1276 1274 17 153 185 445 1498
Fragments arranged by size:
1498 1276 1274 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2649 4349 6061
Size: 274 80 33 51 1443 672 1700 1712
Cuts at: 6061 96
Size: 138
Fragments arranged by size:
1712 1700 1443 672 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2102 2134 3273 3305 6063 6095 2102
Size: 32 1139 32 2758 32 2110
Fragments arranged by size:
2758 2110 1139 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2177 1316
Size: 861 5242
XbaI T'CTAG_A
Cuts at: 3439 3439
Size: 6103
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2192 2195 2899 2192
Size: 3 704 5396
Fragments arranged by size:
5396 704 3
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6103
XmaI C'CCGG_G
Cuts at: 1870 3428 1870
Size: 1558 4545
XmnI GAAnn'nnTTC
Cuts at: 2079 3183 4232 2079
Size: 1104 1049 3950
Fragments arranged by size:
3950 1104 1049
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3911 276
Size: 53 83 186 936 2377 2468
Fragments arranged by size:
2468 2377 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PsrI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI EcoICRI EcoNI EcoO109I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI