Restriction map for
pFN21ASDA0823
(Circular) (Six-base) MAPSORT of: pFN21ASDA0823.seq Check: 1883 from: 1 to: 6430
pFN21ASDA0823 6430 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2459 2459
Size: 6430
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4240 278
Size: 53 83 186 936 2704 2468
Fragments arranged by size:
2704 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3773 1937
Size: 1836 4594
Acc65I G'GTAC_C
Cuts at: 3751 3751
Size: 6430
AclI AA'CG_TT
Cuts at: 1563 4557 4930 1563
Size: 2994 373 3063
Fragments arranged by size:
3063 2994 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2144 2306 3491 4493 5877 6145 1632
Size: 512 162 1185 1002 1384 268 1917
Fragments arranged by size:
1917 1384 1185 1002 512 268 162
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6233
AflIII A'CryG_T
Cuts at: 1203 5329 6306 1203
Size: 4126 977 1327
Fragments arranged by size:
4126 1327 977
AgeI A'CCGG_T
Cuts at: 5261 5261
Size: 6430
AhdI GACnn_n'nnGTC
Cuts at: 5159 5159
Size: 6430
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6430
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2467 2664 2844 3153 3501 1184
Size: 1283 197 180 309 348 4113
Fragments arranged by size:
4113 1283 348 309 197 180
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2924 2956 2924
Size: 32 6398
AlwNI CAG_nnn'CTG
Cuts at: 2062 2122 2686 4173 5745 2062
Size: 60 564 1487 1572 2747
Fragments arranged by size:
2747 1572 1487 564 60
ApaI G_GGCC'C
Cuts at: 2116 2116
Size: 6430
ApaLI G'TGCA_C
Cuts at: 4487 5643 4487
Size: 1156 5274
ApoI r'AATT_y
Cuts at: 1493 3251 3739 3965 4117 6226 1493
Size: 1758 488 226 152 2109 1697
Fragments arranged by size:
2109 1758 1697 488 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4984 160
Size: 872 3952 1606
Fragments arranged by size:
3952 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6430
AvaI C'yCGr_G
Cuts at: 1870 1943 3755 1870
Size: 73 1812 4545
Fragments arranged by size:
4545 1812 73
BamHI G'GATC_C
Cuts at: 3760 4127 3760
Size: 367 6063
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2071 2393 2708 3370 3751
Size: 325 217 627 284 322 315 662 381
Cuts at: 3751 5206 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 662 627 381 325 322 315
284 217
BanII G_rGCy'C
Cuts at: 1391 2116 2763 2817 3313 3452 3749 1391
Size: 725 647 54 496 139 297 4072
Fragments arranged by size:
4072 725 647 496 297 139 54
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5459
BbvCI CC'TCA_GC
Cuts at: 2252 2999 3144 2252
Size: 747 145 5538
Fragments arranged by size:
5538 747 145
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2486 2520 4621 4655 1903
Size: 34 549 34 2101 34 3678
Fragments arranged by size:
3678 2101 549 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2735 4322 5532 2735
Size: 1587 1210 3633
Fragments arranged by size:
3633 1587 1210
BclI T'GATC_A
Cuts at: 1548 2982 1548
Size: 1434 4996
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2231 5041 136
Size: 107 122 71 631 605 559 2810 1525
Fragments arranged by size:
2810 1525 631 605 559 122 107 71
BglII A'GATC_T
Cuts at: 6425 6425
Size: 6430
BlpI GC'TnA_GC
Cuts at: 2757 3500 3847 2757
Size: 743 347 5340
Fragments arranged by size:
5340 743 347
Bme1580I G_kGCm'C
Cuts at: 1790 2076 2116 2137 4491 5647 1790
Size: 286 40 21 2354 1156 2573
Fragments arranged by size:
2573 2354 1156 286 40 21
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6430
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2809 4161 5119
Size: 416 53 83 399 24 1392 1352 958
Cuts at: 5119 442
Size: 1753
Fragments arranged by size:
1753 1392 1352 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6430
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2900 2900
Size: 6430
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2084 2102 2261 2751 2978 3016 3248 3448
Size: 845 18 159 490 227 38 232 200
Cuts at: 3448 3485 5090 1239
Size: 37 1605 2579
Fragments arranged by size:
2579 1605 845 490 232 227 200 159
38 37 18
Bpu10I CC'TnA_GC
Cuts at: 2252 2634 2999 3144 3515 2252
Size: 382 365 145 371 5167
Fragments arranged by size:
5167 382 371 365 145
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3037 4555 5420 5718 3037
Size: 1518 865 298 3749
Fragments arranged by size:
3749 1518 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2567 3179 5093 882
Size: 617 1068 612 1914 2219
Fragments arranged by size:
2219 1914 1068 617 612
BsaAI yAC'GTr
Cuts at: 493 1204 2035 3727 4221 493
Size: 711 831 1692 494 2702
Fragments arranged by size:
2702 1692 831 711 494
BsaBI GATnn'nnATC
Cuts at: 6424 6424
Size: 6430
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2262 3414 4237 4619
Size: 53 83 186 936 729 1152 823 382
Cuts at: 4619 5254 275
Size: 635 1451
Fragments arranged by size:
1451 1152 936 823 729 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3313 4863 5261 5535 5682 6093 3313
Size: 1550 398 274 147 411 3650
Fragments arranged by size:
3650 1550 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2769 2799 2924 2954 1178
Size: 30 165 30 1366 30 125 30 4654
Fragments arranged by size:
4654 1366 165 125 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2372 2753 2945 3698 3707 1186
Size: 1186 381 192 753 9 3909
Fragments arranged by size:
3909 1186 753 381 192 9
BseYI C'CCAG_C
Cuts at: 2115 2808 5633 6129 2115
Size: 693 2825 496 2416
Fragments arranged by size:
2825 2416 693 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2705 3159 1163
Size: 573 969 454 4434
Fragments arranged by size:
4434 969 573 454
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2200 3880 4641 5669 1540
Size: 275 186 199 1680 761 1028 2301
Fragments arranged by size:
2301 1680 1028 761 275 199 186
BsiHKAI G_wGCw'C
Cuts at: 2763 4491 4576 5647 2763
Size: 1728 85 1071 3546
Fragments arranged by size:
3546 1728 1071 85
BsmI GAATG_Cn'
Cuts at: 3941 4034 3941
Size: 93 6337
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2116 2137 2763 2817 3313 3452
Size: 399 286 40 21 626 54 496 139
Cuts at: 3452 3749 4491 4576 5647 1391
Size: 297 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 626 496 399 297 286
139 85 54 40 21
BspEI T'CCGG_A
Cuts at: 6093 6093
Size: 6430
BspHI T'CATG_A
Cuts at: 2985 4319 6049 2985
Size: 1334 1730 3366
Fragments arranged by size:
3366 1730 1334
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2049 2459 2585 2754 3785 844
Size: 1205 410 126 169 1031 3489
Fragments arranged by size:
3489 1205 1031 410 169 126
BsrBI CCG'CTC
Cuts at: 1394 1823 2086 2179 4317 1394
Size: 429 263 93 2138 3507
Fragments arranged by size:
3507 2138 429 263 93
BsrDI GCAATG_nn'
Cuts at: 65 1248 2320 3619 4925 5099 65
Size: 1183 1072 1299 1306 174 1396
Fragments arranged by size:
1396 1306 1299 1183 1072 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5074 5261 1700
Size: 110 3264 187 2869
Fragments arranged by size:
3264 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6430
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6430
BssSI C'ACGA_G
Cuts at: 3270 4490 5502 3270
Size: 1220 1012 4198
Fragments arranged by size:
4198 1220 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2152 3370 794
Size: 1358 1218 3854
Fragments arranged by size:
3854 1358 1218
BstBI TT'CG_AA
Cuts at: 2007 5234 2007
Size: 3227 3203
BstEII G'GTnAC_C
Cuts at: 1184 3637 1184
Size: 2453 3977
BstXI CCAn_nnnn'nTGG
Cuts at: 2035 2035
Size: 6430
BstYI r'GATC_y
Cuts at: 1972 2475 3264 3649 3760 4127 4512 4529 5981
Size: 503 789 385 111 367 385 17 1452
Cuts at: 5981 6067 6079 6425 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 789 503 385 385 367 346
111 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2389 2773 2872 2901 3046 513
Size: 552 957 367 384 99 29 145 3897
Fragments arranged by size:
3897 957 552 384 367 145 99 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5691
Fragments arranged by size:
5691 683 56
BtsI GCAGTG_nn'
Cuts at: 2659 2714 3568 3946 4740 4760 2659
Size: 55 854 378 794 20 4329
Fragments arranged by size:
4329 854 794 378 55 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6430
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6395
DraI TTT'AAA
Cuts at: 3734 3734
Size: 6430
DraIII CAC_nnn'GTG
Cuts at: 3275 3275
Size: 6430
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5437 809
Size: 507 4121 1802
Fragments arranged by size:
4121 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2197 2455
Size: 54 1425 50 275 18 195 172 258
Cuts at: 2455 3339 3408 3540 3877 4766 6395 8
Size: 884 69 132 337 889 1629 43
Fragments arranged by size:
1629 1425 889 884 337 275 258 195
172 132 69 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2197 3877 1537
Size: 275 385 1680 4090
Fragments arranged by size:
4090 1680 385 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2931 4360 1353
Size: 1578 1429 3423
Fragments arranged by size:
3423 1578 1429
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5017 5391 5537 387
Size: 1064 138 3428 374 146 1280
Fragments arranged by size:
3428 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2761 2761
Size: 6430
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2102 2144 2261 2306 2751 2978
Size: 393 452 18 42 117 45 445 227
Cuts at: 2978 3016 3248 3448 3485 3491 4493 5090 5877
Size: 38 232 200 37 6 1002 597 787
Cuts at: 5877 6145 1239
Size: 268 1524
Fragments arranged by size:
1524 1002 787 597 452 445 393 268
232 227 200 117 45 42 38 37
18 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2240 2251 2543 2927 2240
Size: 11 292 384 5743
Fragments arranged by size:
5743 384 292 11
EcoO109I rG'GnC_Cy
Cuts at: 2683 2947 3027 3211 3574 3618 3673 2683
Size: 264 80 184 363 44 55 5440
Fragments arranged by size:
5440 363 264 184 80 55 44
EcoRI G'AATT_C
Cuts at: 3739 4117 3739
Size: 378 6052
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3031 3595 3031
Size: 564 5866
FspI TGC'GCA
Cuts at: 4936 4936
Size: 6430
HaeII r_GCGC'y
Cuts at: 1678 2110 5577 1678
Size: 432 3467 2531
Fragments arranged by size:
3467 2531 432
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3105 3137 5071 5103 5145
Size: 32 135 32 1533 32 1934 32 42
Cuts at: 5145 5177 1373
Size: 32 2626
Fragments arranged by size:
2626 1934 1533 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2512 3774 4020 669
Size: 988 281 574 1262 246 3079
Fragments arranged by size:
3079 1262 988 574 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6430
HpaI GTT'AAC
Cuts at: 4020 4020
Size: 6430
KpnI G_GTAC'C
Cuts at: 3755 3755
Size: 6430
MfeI C'AATT_G
Cuts at: 4029 4029
Size: 6430
MluI A'CGCG_T
Cuts at: 6306 6306
Size: 6430
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3057 5544 5728 1305
Size: 1752 2487 184 2007
Fragments arranged by size:
2487 2007 1752 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2027 3341 3542 10
Size: 54 1425 343 195 1314 201 2898
Fragments arranged by size:
2898 1425 1314 343 201 195 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2973 4388 4747 4906 518
Size: 648 1807 1415 359 159 2042
Fragments arranged by size:
2042 1807 1415 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2059 2122 2675 2756 2828 2998 3133 3143
Size: 453 63 553 81 72 170 135 10
Cuts at: 3143 3154 3481 3645 3846 4523 5671 5916 1606
Size: 11 327 164 201 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 553 453 327 245 201
170 164 135 81 72 63 11 10
NcoI C'CATG_G
Cuts at: 513 1065 2022 2389 2773 2872 2901 513
Size: 552 957 367 384 99 29 4042
Fragments arranged by size:
4042 957 552 384 367 99 29
NdeI CA'TA_TG
Cuts at: 387 2533 387
Size: 2146 4284
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6430
NotI GC'GGCC_GC
Cuts at: 2197 3877 2197
Size: 1680 4750
NspI r_CATG'y
Cuts at: 1132 1229 2530 2825 3289 3788 5333 1132
Size: 97 1301 295 464 499 1545 2229
Fragments arranged by size:
2229 1545 1301 499 464 295 97
PasI CC'CwG_GG
Cuts at: 1348 2704 1348
Size: 1356 5074
PciI A'CATG_T
Cuts at: 5329 5329
Size: 6430
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2686 3126 1108
Size: 1578 440 4412
Fragments arranged by size:
4412 1578 440
PfoI T'CCnGG_A
Cuts at: 2766 2766
Size: 6430
PmeI GTTT'AAAC
Cuts at: 3734 3734
Size: 6430
PmlI CAC'GTG
Cuts at: 2035 2035
Size: 6430
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2924 2956 4499 4531 6012 6044 2924
Size: 32 1543 32 1481 32 3310
Fragments arranged by size:
3310 1543 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2683 2947 3027 3211 3618 2683
Size: 264 80 184 407 5495
Fragments arranged by size:
5495 407 264 184 80
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6430
PsiI TTA'TAA
Cuts at: 4000 4000
Size: 6430
PspOMI G'GGCC_C
Cuts at: 2112 2112
Size: 6430
PstI C_TGCA'G
Cuts at: 830 2058 2446 3490 3782 830
Size: 1228 388 1044 292 3478
Fragments arranged by size:
3478 1228 1044 388 292
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6430
PvuII CAG'CTG
Cuts at: 2059 2122 2756 2828 2998 3143 2059
Size: 63 634 72 170 145 5346
Fragments arranged by size:
5346 634 170 145 72 63
SacI G_AGCT'C
Cuts at: 2763 2763
Size: 6430
SalI G'TCGA_C
Cuts at: 1936 3772 1936
Size: 1836 4594
SbfI CC_TGCA'GG
Cuts at: 3782 3782
Size: 6430
ScaI AGT'ACT
Cuts at: 4678 6390 4678
Size: 1712 4718
SexAI A'CCwGG_T
Cuts at: 2466 2466
Size: 6430
SfcI C'TryA_G
Cuts at: 826 1045 2054 2442 3379 3486 3778 4913 5594
Size: 219 1009 388 937 107 292 1135 681
Cuts at: 5594 5785 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1009 937 681 388 292 219
191 107
SfiI GGCCn_nnn'nGGCC
Cuts at: 2231 2231
Size: 6430
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6430
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6430
SmaI CCC'GGG
Cuts at: 1872 3757 1872
Size: 1885 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3016 4534 5435 5697 820
Size: 197 926 1073 1518 901 262 1553
Fragments arranged by size:
1553 1518 1073 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3727 4221 493
Size: 3234 494 2702
Fragments arranged by size:
3234 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6430
SphI G_CATG'C
Cuts at: 1132 2825 3788 1132
Size: 1693 963 3774
Fragments arranged by size:
3774 1693 963
SspI AAT'ATT
Cuts at: 5 52 4354 5
Size: 47 4302 2081
Fragments arranged by size:
4302 2081 47
StuI AGG'CCT
Cuts at: 1517 2246 1517
Size: 729 5701
StyI C'CwwG_G
Cuts at: 513 1065 2022 2389 2409 2773 2872 2901 3869
Size: 552 957 367 20 364 99 29 968
Cuts at: 3869 513
Size: 3074
Fragments arranged by size:
3074 968 957 552 367 364 99 29
20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2615 4457 4474 4627 4812 5257 325
Size: 1255 1035 1842 17 153 185 445 1498
Fragments arranged by size:
1842 1498 1255 1035 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4676 6388 96
Size: 274 80 33 51 1443 2699 1712 138
Fragments arranged by size:
2699 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2769 2801 2996 3028 6390 6422 2769
Size: 32 195 32 3362 32 2777
Fragments arranged by size:
3362 2777 195 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6430
XbaI T'CTAG_A
Cuts at: 3766 3766
Size: 6430
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3685 3685
Size: 6430
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6430
XmaI C'CCGG_G
Cuts at: 1870 3755 1870
Size: 1885 4545
XmnI GAAnn'nnTTC
Cuts at: 4559 4559
Size: 6430
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4238 276
Size: 53 83 186 936 2704 2468
Fragments arranged by size:
2704 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII
SanDI SapI SfoI SrfI SwaI