Restriction map for
pFN21ASDA0821
(Circular) (Six-base) MAPSORT of: pFN21ASDA0821.seq Check: 3043 from: 1 to: 9151
pFN21ASDA0821 9151 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3943 5583 3943
Size: 1640 7511
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2187 3732 3984 6961
Size: 53 83 186 936 651 1545 252 2977
Cuts at: 6961 278
Size: 2468
Fragments arranged by size:
2977 2468 1545 936 651 252 186 83
53
AccI GT'mk_AC
Cuts at: 1937 2676 3568 4472 6494 1937
Size: 739 892 904 2022 4594
Fragments arranged by size:
4594 2022 904 892 739
Acc65I G'GTAC_C
Cuts at: 5558 6472 5558
Size: 914 8237
AclI AA'CG_TT
Cuts at: 1563 7278 7651 1563
Size: 5715 373 3063
Fragments arranged by size:
5715 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 7214 8598 8866 1632
Size: 5582 1384 268 1917
Fragments arranged by size:
5582 1917 1384 268
AfeI AGC'GCT
Cuts at: 2960 2960
Size: 9151
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8954
AflIII A'CryG_T
Cuts at: 1203 3439 3441 4517 8050 9027 1203
Size: 2236 2 1076 3533 977 1327
Fragments arranged by size:
3533 2236 1327 1076 977 2
AgeI A'CCGG_T
Cuts at: 7982 7982
Size: 9151
AhdI GACnn_n'nnGTC
Cuts at: 7880 7880
Size: 9151
AleI CACnn'nnGTG
Cuts at: 1166 2305 4616 1166
Size: 1139 2311 5701
Fragments arranged by size:
5701 2311 1139
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5372 1184
Size: 4188 4963
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4094 4126 4094
Size: 32 9119
AlwNI CAG_nnn'CTG
Cuts at: 3615 3763 3793 4105 6894 8466 3615
Size: 148 30 312 2789 1572 4300
Fragments arranged by size:
4300 2789 1572 312 148 30
ApaI G_GGCC'C
Cuts at: 3602 5819 5930 5997 6070 6237 6408 3602
Size: 2217 111 67 73 167 171 6345
Fragments arranged by size:
6345 2217 171 167 111 73 67
ApaLI G'TGCA_C
Cuts at: 3956 4859 5333 5888 7208 8364 3956
Size: 903 474 555 1320 1156 4743
Fragments arranged by size:
4743 1320 1156 903 555 474
ApoI r'AATT_y
Cuts at: 1493 5466 6460 6686 6838 8947 1493
Size: 3973 994 226 152 2109 1697
Fragments arranged by size:
3973 2109 1697 994 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7705 160
Size: 872 6673 1606
Fragments arranged by size:
6673 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9151
AvaI C'yCGr_G
Cuts at: 1870 1943 3468 3543 3594 3705 4129 4279 5390
Size: 73 1525 75 51 111 424 150 1111
Cuts at: 5390 5804 5829 5996 6063 6476 1870
Size: 414 25 167 67 413 4545
Fragments arranged by size:
4545 1525 1111 424 414 413 167 150
111 75 73 67 51 25
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5542 5575 5542
Size: 33 9118
BamHI G'GATC_C
Cuts at: 2559 5405 6481 6848 2559
Size: 2846 1076 367 4862
Fragments arranged by size:
4862 2846 1076 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2169 2684 3331 3356 3995
Size: 325 217 627 382 515 647 25 639
Cuts at: 3995 4295 4991 5558 5609 6128 6472 7927 618
Size: 300 696 567 51 519 344 1455 1842
Fragments arranged by size:
1842 1455 696 647 639 627 567 519
515 382 344 325 300 217 51 25
BanII G_rGCy'C
Cuts at: 1391 2112 2472 3456 3468 3585 3602 3713 5819
Size: 721 360 984 12 117 17 111 2106
Cuts at: 5819 5930 5946 5997 6070 6237 6408 6470 1391
Size: 111 16 51 73 167 171 62 4072
Fragments arranged by size:
4072 2106 984 721 360 171 167 117
111 111 73 62 51 17 16 12
BbeI G_GCGC'C
Cuts at: 3999 4299 6132 3999
Size: 300 1833 7018
Fragments arranged by size:
7018 1833 300
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2425 2683 4195 4351 5287 5665 928
Size: 971 526 258 1512 156 936 378 4414
Fragments arranged by size:
4414 1512 971 936 526 378 258 156
BbvCI CC'TCA_GC
Cuts at: 3254 3608 5419 3254
Size: 354 1811 6986
Fragments arranged by size:
6986 1811 354
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3209 3243 7342 7376 1903
Size: 34 1272 34 4099 34 3678
Fragments arranged by size:
4099 3678 1272 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7043 8253 7043
Size: 1210 7941
BclI T'GATC_A
Cuts at: 1548 5871 1548
Size: 4323 4828
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4284 4293 4854
Size: 107 122 71 631 605 2612 9 561
Cuts at: 4854 5852 6185 6317 6369 7762 136
Size: 998 333 132 52 1393 1525
Fragments arranged by size:
2612 1525 1393 998 631 605 561 333
132 122 107 71 52 9
BglII A'GATC_T
Cuts at: 4569 9146 4569
Size: 4577 4574
BlpI GC'TnA_GC
Cuts at: 4740 6568 4740
Size: 1828 7323
Bme1580I G_kGCm'C
Cuts at: 1790 2174 2444 2936 3334 3602 3960 4863 5337
Size: 384 270 492 398 268 358 903 474
Cuts at: 5337 5399 5612 5819 5892 5930 5997 6070 6237
Size: 62 213 207 73 38 67 73 167
Cuts at: 6237 6408 7212 8368 1790
Size: 171 804 1156 2573
Fragments arranged by size:
2573 1156 903 804 492 474 398 384
358 270 268 213 207 171 167 73
73 67 62 38
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9151
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2317 3219 3344
Size: 416 53 83 399 24 900 902 125
Cuts at: 3344 3395 6064 6231 6882 7840 442
Size: 51 2669 167 651 958 1753
Fragments arranged by size:
2669 1753 958 902 900 651 416 399
167 125 83 53 51 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9151
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2491 2611 2491
Size: 120 9031
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2408 2486 3224 3372 3941 4007 4085 4418
Size: 1169 78 738 148 569 66 78 333
Cuts at: 4418 4871 5056 5125 6062 7811 1239
Size: 453 185 69 937 1749 2579
Fragments arranged by size:
2579 1749 1169 937 738 569 453 333
185 148 78 78 69 66
Bpu10I CC'TnA_GC
Cuts at: 2096 3254 3450 3608 5419 5820 5940 2096
Size: 1158 196 158 1811 401 120 5307
Fragments arranged by size:
5307 1811 1158 401 196 158 120
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5114 5396 6413 7276 8141 8439 5114
Size: 282 1017 863 865 298 5826
Fragments arranged by size:
5826 1017 865 863 298 282
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7814 882
Size: 617 6315 2219
Fragments arranged by size:
6315 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2548 2611 2937 3019 3442 4518 6448
Size: 711 1344 63 326 82 423 1076 1930
Cuts at: 6448 6942 493
Size: 494 2702
Fragments arranged by size:
2702 1930 1344 1076 711 494 423 326
82 63
BsaBI GATnn'nnATC
Cuts at: 5873 9145 5873
Size: 3272 5879
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2184 2283 3729 3981
Size: 53 83 186 936 651 99 1446 252
Cuts at: 3981 3996 4296 6129 6355 6958 7340 7975 275
Size: 15 300 1833 226 603 382 635 1451
Fragments arranged by size:
1833 1451 1446 936 651 635 603 382
300 252 226 186 99 83 53 15
BsaWI w'CCGG_w
Cuts at: 7584 7982 8256 8403 8814 7584
Size: 398 274 147 411 7921
Fragments arranged by size:
7921 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2126 2156 2381 2411 3605
Size: 30 165 30 723 30 225 30 1194
Cuts at: 3605 3635 3719 3749 4094 4124 5762 5792 6191
Size: 30 84 30 345 30 1638 30 399
Cuts at: 6191 6221 1178
Size: 30 4108
Fragments arranged by size:
4108 1638 1194 723 399 345 225 165
84 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3101 3121 3724 3727 3965 4004 4136 5216
Size: 1915 20 603 3 238 39 132 1080
Cuts at: 5216 5650 6059 6139 1186
Size: 434 409 80 4198
Fragments arranged by size:
4198 1915 1080 603 434 409 238 132
80 39 20 3
BseYI C'CCAG_C
Cuts at: 2805 3235 3370 3407 3424 3501 3780 4459 5173
Size: 430 135 37 17 77 279 679 714
Cuts at: 5173 5200 8354 8850 2805
Size: 27 3154 496 3106
Fragments arranged by size:
3154 3106 714 679 496 430 279 135
77 37 27 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2285 2414 3601 4118 4796 5084 6097
Size: 573 549 129 1187 517 678 288 1013
Cuts at: 6097 1163
Size: 4217
Fragments arranged by size:
4217 1187 1013 678 573 549 517 288
129
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4696 6120 6601 7362 8390 1540
Size: 275 186 2695 1424 481 761 1028 2301
Fragments arranged by size:
2695 2301 1424 1028 761 481 275 186
BsiHKAI G_wGCw'C
Cuts at: 2046 2496 3456 3960 4554 4863 5130 5337 5892
Size: 450 960 504 594 309 267 207 555
Cuts at: 5892 7212 7297 8368 2046
Size: 1320 85 1071 2829
Fragments arranged by size:
2829 1320 1071 960 594 555 504 450
309 267 207 85
BsmI GAATG_Cn'
Cuts at: 2206 3946 4252 6662 6755 2206
Size: 1740 306 2410 93 4602
Fragments arranged by size:
4602 2410 1740 306 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2753 2936 3736 2753
Size: 183 800 8168
Fragments arranged by size:
8168 800 183
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2112 2174 2444 2472 2496 2936
Size: 399 256 66 62 270 28 24 440
Cuts at: 2936 3334 3456 3468 3585 3602 3713 3960 4554
Size: 398 122 12 117 17 111 247 594
Cuts at: 4554 4863 5130 5337 5399 5612 5819 5892 5930
Size: 309 267 207 62 213 207 73 38
Cuts at: 5930 5946 5997 6070 6237 6408 6470 7212 7297
Size: 16 51 73 167 171 62 742 85
Cuts at: 7297 8368 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 594 440 399 398 309
270 267 256 247 213 207 207 171
167 122 117 111 85 73 73 66
62 62 62 51 38 28 24 17
16 12
BspEI T'CCGG_A
Cuts at: 8814 8814
Size: 9151
BspHI T'CATG_A
Cuts at: 7040 8770 7040
Size: 1730 7421
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2283 3943 4878 5583 5907 6201 6506 844
Size: 1439 1660 935 705 324 294 305 3489
Fragments arranged by size:
3489 1660 1439 935 705 324 305 294
BsrBI CCG'CTC
Cuts at: 1394 1823 2754 3322 5382 7038 1394
Size: 429 931 568 2060 1656 3507
Fragments arranged by size:
3507 2060 1656 931 568 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4189 7646 7820 65
Size: 1183 2941 3457 174 1396
Fragments arranged by size:
3457 2941 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2339 3493 3818 4265 4802 6138 7795
Size: 110 529 1154 325 447 537 1336 1657
Cuts at: 7795 7982 1700
Size: 187 2869
Fragments arranged by size:
2869 1657 1336 1154 537 529 447 325
187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9151
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9151
BssSI C'ACGA_G
Cuts at: 2483 2596 6365 7211 8223 2483
Size: 113 3769 846 1012 3411
Fragments arranged by size:
3769 3411 1012 846 113
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2325 2461 3511 3793 794
Size: 1531 136 1050 282 6152
Fragments arranged by size:
6152 1531 1050 282 136
BstBI TT'CG_AA
Cuts at: 2007 7955 2007
Size: 5948 3203
BstEII G'GTnAC_C
Cuts at: 1184 2074 2534 5092 6433 1184
Size: 890 460 2558 1341 3902
Fragments arranged by size:
3902 2558 1341 890 460
BstXI CCAn_nnnn'nTGG
Cuts at: 2653 3691 5149 2653
Size: 1038 1458 6655
Fragments arranged by size:
6655 1458 1038
BstYI r'GATC_y
Cuts at: 1972 2559 4569 5405 6481 6848 7233 7250 8702
Size: 587 2010 836 1076 367 385 17 1452
Cuts at: 8702 8788 8800 9146 1972
Size: 86 12 346 1977
Fragments arranged by size:
2010 1977 1452 1076 836 587 385 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 6228 6228
Size: 9151
BtgI C'CryG_G
Cuts at: 513 1065 2022 3879 3945 4239 4386 5562 5602
Size: 552 957 1857 66 294 147 1176 40
Cuts at: 5602 513
Size: 4062
Fragments arranged by size:
4062 1857 1176 957 552 294 147 66
40
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2250 3664 4309 4945 5167 5617
Size: 683 56 1024 1414 645 636 222 450
Cuts at: 5617 487
Size: 4021
Fragments arranged by size:
4021 1414 1024 683 645 636 450 222
56
BtsI GCAGTG_nn'
Cuts at: 2046 2273 2918 5311 6667 7461 7481 2046
Size: 227 645 2393 1356 794 20 3716
Fragments arranged by size:
3716 2393 1356 794 645 227 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9151
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9116
DraI TTT'AAA
Cuts at: 6455 6455
Size: 9151
DraIII CAC_nnn'GTG
Cuts at: 2325 3352 2325
Size: 1027 8124
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2485 8158 809
Size: 507 1169 5673 1802
Fragments arranged by size:
5673 1802 1169 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2082 2613 3491
Size: 54 1425 50 275 18 252 531 878
Cuts at: 3491 3495 3672 3908 3963 4008 4263 4647 5919
Size: 4 177 236 55 45 255 384 1272
Cuts at: 5919 6117 6160 6598 7487 9116 8
Size: 198 43 438 889 1629 43
Fragments arranged by size:
1629 1425 1272 889 878 531 438 384
275 255 252 236 198 177 55 54
50 45 43 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 6117 6598 1537
Size: 275 4305 481 4090
Fragments arranged by size:
4305 4090 481 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3461 4235 4736 4754 5234 5270 5976 7081
Size: 2108 774 501 18 480 36 706 1105
Cuts at: 7081 1353
Size: 3423
Fragments arranged by size:
3423 2108 1105 774 706 501 480 36
18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2826 3314 7738 8112 8258 387
Size: 1064 138 1237 488 4424 374 146 1280
Fragments arranged by size:
4424 1280 1237 1064 488 374 146 138
EcoICRI GAG'CTC
Cuts at: 3454 3454
Size: 9151
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2408 2486 3224 3372 3941 4007 4085
Size: 393 776 78 738 148 569 66 78
Cuts at: 4085 4418 4871 5056 5125 6062 7214 7811 8598
Size: 333 453 185 69 937 1152 597 787
Cuts at: 8598 8866 1239
Size: 268 1524
Fragments arranged by size:
1524 1152 937 787 776 738 597 569
453 393 333 268 185 148 78 78
69 66
EcoNI CCTnn'n_nnAGG
Cuts at: 2456 3989 5961 2456
Size: 1533 1972 5646
Fragments arranged by size:
5646 1972 1533
EcoO109I rG'GnC_Cy
Cuts at: 2373 2448 2518 2992 3374 3525 3598 3599 3790
Size: 75 70 474 382 151 73 1 191
Cuts at: 3790 5454 5815 5816 5926 5927 6037 6233 6244
Size: 1664 361 1 110 1 110 196 11
Cuts at: 6244 6404 2373
Size: 160 5120
Fragments arranged by size:
5120 1664 474 382 361 196 191 160
151 110 110 75 73 70 11 1
1 1
EcoRI G'AATT_C
Cuts at: 5466 6460 6838 5466
Size: 994 378 7779
Fragments arranged by size:
7779 994 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4429 5728 4429
Size: 1299 7852
FseI GG_CCGG'CC
Cuts at: 3497 3497
Size: 9151
FspI TGC'GCA
Cuts at: 7657 7657
Size: 9151
HaeII r_GCGC'y
Cuts at: 1678 2962 3999 4299 5174 6132 8298 1678
Size: 1284 1037 300 875 958 2166 2531
Fragments arranged by size:
2531 2166 1284 1037 958 875 300
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2483 2515 2945 2977 3719
Size: 32 135 32 911 32 430 32 742
Cuts at: 3719 3751 3797 3829 6191 6223 7792 7824 7866
Size: 32 46 32 2362 32 1569 32 42
Cuts at: 7866 7898 1373
Size: 32 2626
Fragments arranged by size:
2626 2362 1569 911 742 430 135 46
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3139 3634 4473 5074 6495 6741
Size: 988 281 1201 495 839 601 1421 246
Cuts at: 6741 669
Size: 3079
Fragments arranged by size:
3079 1421 1201 988 839 601 495 281
246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9151
HpaI GTT'AAC
Cuts at: 6741 6741
Size: 9151
KasI G'GCGC_C
Cuts at: 3995 4295 6128 3995
Size: 300 1833 7018
Fragments arranged by size:
7018 1833 300
KpnI G_GTAC'C
Cuts at: 5562 6476 5562
Size: 914 8237
MfeI C'AATT_G
Cuts at: 6750 6750
Size: 9151
MluI A'CGCG_T
Cuts at: 9027 9027
Size: 9151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2994 4524 6084 8265 8449 1305
Size: 1689 1530 1560 2181 184 2007
Fragments arranged by size:
2181 2007 1689 1560 1530 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4649 10
Size: 54 1425 343 2817 4512
Fragments arranged by size:
4512 2817 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2192 2305 2546 3515 4416 4616 7109
Size: 648 1026 113 241 969 901 200 2493
Cuts at: 7109 7468 7627 518
Size: 359 159 2042
Fragments arranged by size:
2493 2042 1026 969 901 648 359 241
200 159 113
MspA1I CmG'CkG
Cuts at: 1606 2524 2805 2905 3163 3370 3760 3784 4150
Size: 918 281 100 258 207 390 24 366
Cuts at: 4150 6183 6430 6567 7244 8392 8637 1606
Size: 2033 247 137 677 1148 245 2120
Fragments arranged by size:
2120 2033 1148 918 677 390 366 281
258 247 245 207 137 100 24
NaeI GCC'GGC
Cuts at: 2341 3495 3820 4267 4804 6140 2341
Size: 1154 325 447 537 1336 5352
Fragments arranged by size:
5352 1336 1154 537 447 325
NarI GG'CG_CC
Cuts at: 3996 4296 6129 3996
Size: 300 1833 7018
Fragments arranged by size:
7018 1833 300
NcoI C'CATG_G
Cuts at: 513 1065 2022 5562 513
Size: 552 957 3540 4102
Fragments arranged by size:
4102 3540 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9151
NgoMIV G'CCGG_C
Cuts at: 2339 3493 3818 4265 4802 6138 2339
Size: 1154 325 447 537 1336 5352
Fragments arranged by size:
5352 1336 1154 537 447 325
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9151
NotI GC'GGCC_GC
Cuts at: 6598 6598
Size: 9151
NruI TCG'CGA
Cuts at: 3153 3153
Size: 9151
NspI r_CATG'y
Cuts at: 1132 1229 3514 6509 8054 1132
Size: 97 2285 2995 1545 2229
Fragments arranged by size:
2995 2285 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2444 3627 4289 5684 1348
Size: 1096 1183 662 1395 4815
Fragments arranged by size:
4815 1395 1183 1096 662
PciI A'CATG_T
Cuts at: 8050 8050
Size: 9151
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2329 1108
Size: 1221 7930
PfoI T'CCnGG_A
Cuts at: 3771 3926 3984 4062 3771
Size: 155 58 78 8860
Fragments arranged by size:
8860 155 78 58
PmeI GTTT'AAAC
Cuts at: 6455 6455
Size: 9151
PmlI CAC'GTG
Cuts at: 2937 3442 4518 2937
Size: 505 1076 7570
Fragments arranged by size:
7570 1076 505
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4094 4126 7220 7252 8733 8765 4094
Size: 32 3094 32 1481 32 4480
Fragments arranged by size:
4480 3094 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2373 2448 2518 2992 3374 5454 2373
Size: 75 70 474 382 2080 6070
Fragments arranged by size:
6070 2080 474 382 75 70
PshAI GACnn'nnGTC
Cuts at: 1613 2364 1613
Size: 751 8400
PsiI TTA'TAA
Cuts at: 6721 6721
Size: 9151
PspOMI G'GGCC_C
Cuts at: 3598 5815 5926 5993 6066 6233 6404 3598
Size: 2217 111 67 73 167 171 6345
Fragments arranged by size:
6345 2217 171 167 111 73 67
PspXI vC'TCGA_Gb
Cuts at: 3543 3543
Size: 9151
PstI C_TGCA'G
Cuts at: 830 2457 2529 3789 4529 4689 5598 6336 6503
Size: 1627 72 1260 740 160 909 738 167
Cuts at: 6503 830
Size: 3478
Fragments arranged by size:
3478 1627 1260 909 740 738 167 160
72
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9151
PvuII CAG'CTG
Cuts at: 2905 3163 3370 3760 3784 4150 6430 2905
Size: 258 207 390 24 366 2280 5626
Fragments arranged by size:
5626 2280 390 366 258 207 24
SacI G_AGCT'C
Cuts at: 3456 3456
Size: 9151
SalI G'TCGA_C
Cuts at: 1936 4471 6493 1936
Size: 2535 2022 4594
Fragments arranged by size:
4594 2535 2022
SanDI GG'GwC_CC
Cuts at: 2448 5454 2448
Size: 3006 6145
SapI GCTCTTCn'nnn_
Cuts at: 3461 4754 3461
Size: 1293 7858
SbfI CC_TGCA'GG
Cuts at: 6336 6503 6336
Size: 167 8984
ScaI AGT'ACT
Cuts at: 4916 7399 9111 4916
Size: 2483 1712 4956
Fragments arranged by size:
4956 2483 1712
SexAI A'CCwGG_T
Cuts at: 3636 5076 6345 3636
Size: 1440 1269 6442
Fragments arranged by size:
6442 1440 1269
SfcI C'TryA_G
Cuts at: 826 1045 2453 2525 3196 3785 4525 4685 5594
Size: 219 1408 72 671 589 740 160 909
Cuts at: 5594 6332 6499 7634 8315 8506 826
Size: 738 167 1135 681 191 1471
Fragments arranged by size:
1471 1408 1135 909 740 738 681 671
589 219 191 167 160 72
SfiI GGCCn_nnn'nGGCC
Cuts at: 4284 4284
Size: 9151
SfoI GGC'GCC
Cuts at: 3997 4297 6130 3997
Size: 300 1833 7018
Fragments arranged by size:
7018 1833 300
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9151
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9151
SmaI CCC'GGG
Cuts at: 1872 3596 3707 4281 5392 5806 5998 6065 6478
Size: 1724 111 574 1111 414 192 67 413
Cuts at: 6478 1872
Size: 4545
Fragments arranged by size:
4545 1724 1111 574 414 413 192 111
67
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3543 5129 5411 6392 7255 8156
Size: 197 926 1600 1586 282 981 863 901
Cuts at: 8156 8418 820
Size: 262 1553
Fragments arranged by size:
1600 1586 1553 981 926 901 863 282
262 197
SnaBI TAC'GTA
Cuts at: 493 6448 6942 493
Size: 5955 494 2702
Fragments arranged by size:
5955 2702 494
SpeI A'CTAG_T
Cuts at: 152 4876 152
Size: 4724 4427
SphI G_CATG'C
Cuts at: 1132 3514 6509 1132
Size: 2382 2995 3774
Fragments arranged by size:
3774 2995 2382
SspI AAT'ATT
Cuts at: 5 52 7075 5
Size: 47 7023 2081
Fragments arranged by size:
7023 2081 47
StuI AGG'CCT
Cuts at: 1517 2094 5003 6390 1517
Size: 577 2909 1387 4278
Fragments arranged by size:
4278 2909 1387 577
StyI C'CwwG_G
Cuts at: 513 1065 2022 2089 3534 3576 4047 4477 5562
Size: 552 957 67 1445 42 471 430 1085
Cuts at: 5562 5712 5922 6590 513
Size: 150 210 668 3074
Fragments arranged by size:
3074 1445 1085 957 668 552 471 430
210 150 67 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2102 2336 2834 3344 3363 3515 4594
Size: 1255 522 234 498 510 19 152 1079
Cuts at: 4594 4606 4706 4916 7178 7195 7348 7533 7978
Size: 12 100 210 2262 17 153 185 445
Cuts at: 7978 325
Size: 1498
Fragments arranged by size:
2262 1498 1255 1079 522 510 498 445
234 210 185 153 152 100 19 17
12
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4914 5343 7397
Size: 274 80 33 51 1443 2937 429 2054
Cuts at: 7397 9109 96
Size: 1712 138
Fragments arranged by size:
2937 2054 1712 1443 429 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2041 2073 2126 2158 9111 9143 2041
Size: 32 53 32 6953 32 2049
Fragments arranged by size:
6953 2049 53 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2405 4016 4373 5506 1316
Size: 1089 1611 357 1133 4961
Fragments arranged by size:
4961 1611 1133 1089 357
XbaI T'CTAG_A
Cuts at: 6487 6487
Size: 9151
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2977 3349 3367 4054 4258 5710 2977
Size: 372 18 687 204 1452 6418
Fragments arranged by size:
6418 1452 687 372 204 18
XhoI C'TCGA_G
Cuts at: 1943 3543 1943
Size: 1600 7551
XmaI C'CCGG_G
Cuts at: 1870 3594 3705 4279 5390 5804 5996 6063 6476
Size: 1724 111 574 1111 414 192 67 413
Cuts at: 6476 1870
Size: 4545
Fragments arranged by size:
4545 1724 1111 574 414 413 192 111
67
XmnI GAAnn'nnTTC
Cuts at: 2432 4412 7280 2432
Size: 1980 2868 4303
Fragments arranged by size:
4303 2868 1980
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2185 3730 3982 6959
Size: 53 83 186 936 651 1545 252 2977
Cuts at: 6959 276
Size: 2468
Fragments arranged by size:
2977 2468 1545 936 651 252 186 83
53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BfrBI BsiWI BstZ17I EcoRV FspAI NsiI
PacI PsrI RsrII SacII SrfI SwaI