Restriction map for
pFN21ASDA0814
(Circular) (Six-base) MAPSORT of: pFN21ASDA0814.seq Check: 9738 from: 1 to: 9298
pFN21ASDA0814 9298 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4904 4937 5060 5327 5456 5566 4904
Size: 33 123 267 129 110 8636
Fragments arranged by size:
8636 267 129 123 110 33
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7108 278
Size: 53 83 186 936 5572 2468
Fragments arranged by size:
5572 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6641 1937
Size: 4704 4594
Acc65I G'GTAC_C
Cuts at: 6619 6619
Size: 9298
AclI AA'CG_TT
Cuts at: 1563 7425 7798 1563
Size: 5862 373 3063
Fragments arranged by size:
5862 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2366 3041 4390 4560 5317 7361 8745 9013
Size: 734 675 1349 170 757 2044 1384 268
Cuts at: 9013 1632
Size: 1917
Fragments arranged by size:
2044 1917 1384 1349 757 734 675 268
170
AfeI AGC'GCT
Cuts at: 2559 2559
Size: 9298
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9101
AflIII A'CryG_T
Cuts at: 1203 2805 3213 4413 5076 6189 8197 9174 1203
Size: 1602 408 1200 663 1113 2008 977 1327
Fragments arranged by size:
2008 1602 1327 1200 1113 977 663 408
AgeI A'CCGG_T
Cuts at: 8129 8129
Size: 9298
AhdI GACnn_n'nnGTC
Cuts at: 6380 8027 6380
Size: 1647 7651
AleI CACnn'nnGTG
Cuts at: 1166 2495 3566 4938 5054 1166
Size: 1329 1071 1372 116 5410
Fragments arranged by size:
5410 1372 1329 1071 116
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4403 5447 1184
Size: 3219 1044 5035
Fragments arranged by size:
5035 3219 1044
AlwNI CAG_nnn'CTG
Cuts at: 2344 3082 3226 3589 3778 5770 5998 7041 8613
Size: 738 144 363 189 1992 228 1043 1572
Cuts at: 8613 2344
Size: 3029
Fragments arranged by size:
3029 1992 1572 1043 738 363 228 189
144
ApaI G_GGCC'C
Cuts at: 3409 5284 5419 6474 3409
Size: 1875 135 1055 6233
Fragments arranged by size:
6233 1875 1055 135
ApaLI G'TGCA_C
Cuts at: 3809 4207 6523 7355 8511 3809
Size: 398 2316 832 1156 4596
Fragments arranged by size:
4596 2316 1156 832 398
ApoI r'AATT_y
Cuts at: 1493 6607 6833 6985 9094 1493
Size: 5114 226 152 2109 1697
Fragments arranged by size:
5114 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7852 160
Size: 872 6820 1606
Fragments arranged by size:
6820 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9298
AvaI C'yCGr_G
Cuts at: 1870 1943 2030 2190 2467 3539 6126 6623 1870
Size: 73 87 160 277 1072 2587 497 4545
Fragments arranged by size:
4545 2587 1072 497 277 160 87 73
AvrII C'CTAG_G
Cuts at: 4085 4085
Size: 9298
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5065 5098 5065
Size: 33 9265
BamHI G'GATC_C
Cuts at: 2463 6628 6995 2463
Size: 4165 367 4766
Fragments arranged by size:
4766 4165 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2048 2330 4089 4925 6439
Size: 325 217 627 261 282 1759 836 1514
Cuts at: 6439 6619 8074 618
Size: 180 1455 1842
Fragments arranged by size:
1842 1759 1514 1455 836 627 325 282
261 217 180
BanII G_rGCy'C
Cuts at: 1391 2274 2832 3288 3409 4190 4251 4689 5284
Size: 883 558 456 121 781 61 438 595
Cuts at: 5284 5419 5445 6312 6474 6617 1391
Size: 135 26 867 162 143 4072
Fragments arranged by size:
4072 883 867 781 595 558 456 438
162 143 135 121 61 26
BbeI G_GCGC'C
Cuts at: 2052 2334 2052
Size: 282 9016
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2307 4607 6508 928
Size: 971 408 2300 1901 3718
Fragments arranged by size:
3718 2300 1901 971 408
BbvCI CC'TCA_GC
Cuts at: 3857 4454 3857
Size: 597 8701
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2872 2906 5027 5061 7489 7523 1903
Size: 34 935 34 2121 34 2428 34 3678
Fragments arranged by size:
3678 2428 2121 935 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7190 8400 7190
Size: 1210 8088
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9298
BfrBI ATG'CAT
Cuts at: 5937 6416 5937
Size: 479 8819
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2069 3837 4980
Size: 107 122 71 631 605 397 1768 1143
Cuts at: 4980 5328 5463 6093 6330 7909 136
Size: 348 135 630 237 1579 1525
Fragments arranged by size:
1768 1579 1525 1143 631 630 605 397
348 237 135 122 107 71
BglII A'GATC_T
Cuts at: 9293 9293
Size: 9298
BlpI GC'TnA_GC
Cuts at: 2211 4184 6259 6715 2211
Size: 1973 2075 456 4794
Fragments arranged by size:
4794 2075 1973 456
Bme1580I G_kGCm'C
Cuts at: 1790 2813 3409 3539 3563 3813 4211 4326 4887
Size: 1023 596 130 24 250 398 115 561
Cuts at: 4887 5284 5419 5906 6444 6474 6527 7359 8515
Size: 397 135 487 538 30 53 832 1156
Cuts at: 8515 1790
Size: 2573
Fragments arranged by size:
2573 1156 1023 832 596 561 538 487
398 397 250 135 130 115 53 30
24
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9298
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3001 5278 5413
Size: 416 53 83 399 24 1584 2277 135
Cuts at: 5413 6186 6469 7029 7987 442
Size: 773 283 560 958 1753
Fragments arranged by size:
2277 1753 1584 958 773 560 416 399
283 135 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9298
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2124 5706 2124
Size: 3582 5716
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2094 3048 3362 3683 3821 4771 5660 5801
Size: 855 954 314 321 138 950 889 141
Cuts at: 5801 7958 1239
Size: 2157 2579
Fragments arranged by size:
2579 2157 954 950 889 855 321 314
141 138
Bpu10I CC'TnA_GC
Cuts at: 3478 3857 4367 4439 4454 4683 5142 3478
Size: 379 510 72 15 229 459 7634
Fragments arranged by size:
7634 510 459 379 229 72 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2258 4094 7423 8288 8586 2258
Size: 1836 3329 865 298 2970
Fragments arranged by size:
3329 2970 1836 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3797 7961 882
Size: 617 2298 4164 2219
Fragments arranged by size:
4164 2298 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2806 2877 5077 5527 5659 6595 7089
Size: 711 1602 71 2200 450 132 936 494
Cuts at: 7089 493
Size: 2702
Fragments arranged by size:
2702 2200 1602 936 711 494 450 132
71
BsaBI GATnn'nnATC
Cuts at: 4121 9292 4121
Size: 5171 4127
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2049 2331 7105 7487
Size: 53 83 186 936 516 282 4774 382
Cuts at: 7487 8122 275
Size: 635 1451
Fragments arranged by size:
4774 1451 936 635 516 382 282 186
83 53
BsaWI w'CCGG_w
Cuts at: 7731 8129 8403 8550 8961 7731
Size: 398 274 147 411 8068
Fragments arranged by size:
8068 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2792 2822 2840 2870 3156
Size: 30 165 30 1389 30 18 30 286
Cuts at: 3156 3186 3230 3260 1178
Size: 30 44 30 7216
Fragments arranged by size:
7216 1389 286 165 44 30 30 30
30 30 30 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3164 3167 3874 4181 6526 1186
Size: 1978 3 707 307 2345 3958
Fragments arranged by size:
3958 2345 1978 707 307 3
BseYI C'CCAG_C
Cuts at: 2170 2267 2812 4582 8501 8997 2170
Size: 97 545 1770 3919 496 2471
Fragments arranged by size:
3919 2471 1770 545 496 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2810 2857 2899 3791 4861 5453 6010
Size: 573 1074 47 42 892 1070 592 557
Cuts at: 6010 1163
Size: 4451
Fragments arranged by size:
4451 1074 1070 892 592 573 557 47
42
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6748 7509 8537 1540
Size: 275 186 4747 761 1028 2301
Fragments arranged by size:
4747 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2417 3813 4211 6348 6527 7359 7444 8515 2417
Size: 1396 398 2137 179 832 85 1071 3200
Fragments arranged by size:
3200 2137 1396 1071 832 398 179 85
BsmI GAATG_Cn'
Cuts at: 6809 6902 6809
Size: 93 9205
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2274 2417 2813 2832 3288 3409 3539
Size: 399 484 143 396 19 456 121 130
Cuts at: 3539 3563 3813 4190 4211 4251 4326 4689 4887
Size: 24 250 377 21 40 75 363 198
Cuts at: 4887 5284 5419 5445 5906 6312 6348 6444 6474
Size: 397 135 26 461 406 36 96 30
Cuts at: 6474 6527 6617 7359 7444 8515 1391
Size: 53 90 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 484 461 456 406 399
397 396 377 363 250 198 143 135
130 121 96 90 85 75 53 40
36 30 26 24 21 19
BspEI T'CCGG_A
Cuts at: 8961 8961
Size: 9298
BspHI T'CATG_A
Cuts at: 7187 8917 7187
Size: 1730 7568
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2147 2514 3234 3492 3639 3705 4904 4937
Size: 1303 367 720 258 147 66 1199 33
Cuts at: 4937 5060 5327 5456 5566 6653 844
Size: 123 267 129 110 1087 3489
Fragments arranged by size:
3489 1303 1199 1087 720 367 267 258
147 129 123 110 66 33
BsrBI CCG'CTC
Cuts at: 1394 1823 6054 7185 1394
Size: 429 4231 1131 3507
Fragments arranged by size:
4231 3507 1131 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2848 4525 4780 7793 7967 65
Size: 1183 1600 1677 255 3013 174 1396
Fragments arranged by size:
3013 1677 1600 1396 1183 255 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3899 4449 5471 5879 5910 6101 7942
Size: 110 2089 550 1022 408 31 191 1841
Cuts at: 7942 8129 1700
Size: 187 2869
Fragments arranged by size:
2869 2089 1841 1022 550 408 191 187
110 31
BsrGI T'GTAC_A
Cuts at: 96 5703 96
Size: 5607 3691
BssHII G'CGCG_C
Cuts at: 1925 2059 1925
Size: 134 9164
BssSI C'ACGA_G
Cuts at: 3530 7358 8370 3530
Size: 3828 1012 4458
Fragments arranged by size:
4458 3828 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3516 4779 4875 6024 6033 6422 794
Size: 2722 1263 96 1149 9 389 3670
Fragments arranged by size:
3670 2722 1263 1149 389 96 9
BstBI TT'CG_AA
Cuts at: 2007 8102 2007
Size: 6095 3203
BstEII G'GTnAC_C
Cuts at: 1184 2614 1184
Size: 1430 7868
BstXI CCAn_nnnn'nTGG
Cuts at: 4955 5056 4955
Size: 101 9197
BstYI r'GATC_y
Cuts at: 1972 2454 2463 2574 2640 6628 6995 7380 7397
Size: 482 9 111 66 3988 367 385 17
Cuts at: 7397 8849 8935 8947 9293 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
3988 1977 1452 482 385 367 346 111
86 66 17 12 9
BtgI C'CryG_G
Cuts at: 513 1065 2022 2164 2482 3826 4513 4689 5275
Size: 552 957 142 318 1344 687 176 586
Cuts at: 5275 5364 5929 6057 6157 6277 513
Size: 89 565 128 100 120 3534
Fragments arranged by size:
3534 1344 957 687 586 565 552 318
176 142 128 120 100 89
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3081 4246 4648 487
Size: 683 56 1855 1165 402 5137
Fragments arranged by size:
5137 1855 1165 683 402 56
BtsI GCAGTG_nn'
Cuts at: 4211 5838 6290 6346 6814 7608 7628 4211
Size: 1627 452 56 468 794 20 5881
Fragments arranged by size:
5881 1627 794 468 452 56 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9298
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9263
DraI TTT'AAA
Cuts at: 6602 6602
Size: 9298
DraIII CAC_nnn'GTG
Cuts at: 2133 5137 5743 6033 2133
Size: 3004 606 290 5398
Fragments arranged by size:
5398 3004 606 290
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2309 8305 809
Size: 507 993 5996 1802
Fragments arranged by size:
5996 1802 993 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2731 3366 4134
Size: 54 1425 50 275 18 901 635 768
Cuts at: 4134 4764 5580 6142 6745 7634 9263 8
Size: 630 816 562 603 889 1629 43
Fragments arranged by size:
1629 1425 901 889 816 768 635 630
603 562 275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6745 1537
Size: 275 4933 4090
Fragments arranged by size:
4933 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3293 4055 6543 7228 1353
Size: 1940 762 2488 685 3423
Fragments arranged by size:
3423 2488 1940 762 685
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2798 4081 4327 5079 5530 6386
Size: 1064 138 1209 1283 246 752 451 856
Cuts at: 6386 6520 7885 8259 8405 387
Size: 134 1365 374 146 1280
Fragments arranged by size:
1365 1283 1280 1209 1064 856 752 451
374 246 146 138 134
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2094 2366 3041 3048 3362 3683 3821
Size: 393 462 272 675 7 314 321 138
Cuts at: 3821 4390 4560 4771 5317 5660 5801 7361 7958
Size: 569 170 211 546 343 141 1560 597
Cuts at: 7958 8745 9013 1239
Size: 787 268 1524
Fragments arranged by size:
1560 1524 787 675 597 569 546 462
393 343 321 314 272 268 211 170
141 138 7
EcoO109I rG'GnC_Cy
Cuts at: 2341 3079 3223 3405 3963 4822 5280 5415 5980
Size: 738 144 182 558 859 458 135 565
Cuts at: 5980 6130 6180 6471 2341
Size: 150 50 291 5168
Fragments arranged by size:
5168 859 738 565 558 458 291 182
150 144 135 50
EcoRI G'AATT_C
Cuts at: 6607 6985 6607
Size: 378 8920
EcoRV GAT'ATC
Cuts at: 3051 3051
Size: 9298
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2278 3304 2278
Size: 1026 8272
FspI TGC'GCA
Cuts at: 5951 7804 5951
Size: 1853 7445
FspAI rTGC'GCAy
Cuts at: 5951 5951
Size: 9298
HaeII r_GCGC'y
Cuts at: 1678 2052 2075 2087 2218 2334 2561 4583 8445
Size: 374 23 12 131 116 227 2022 3862
Cuts at: 8445 1678
Size: 2531
Fragments arranged by size:
3862 2531 2022 374 227 131 116 23
12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2632 2664 2819 2851 4547
Size: 32 135 32 1060 32 155 32 1696
Cuts at: 4547 4579 6366 6378 6398 6410 6521 6553 7939
Size: 32 1787 12 20 12 111 32 1386
Cuts at: 7939 7971 8013 8045 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1787 1696 1386 1060 155 135 111
42 32 32 32 32 32 32 32
32 20 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 5503 6642 6888 669
Size: 988 281 3565 1139 246 3079
Fragments arranged by size:
3565 3079 1139 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9298
HpaI GTT'AAC
Cuts at: 6888 6888
Size: 9298
KasI G'GCGC_C
Cuts at: 2048 2330 2048
Size: 282 9016
KpnI G_GTAC'C
Cuts at: 6623 6623
Size: 9298
MfeI C'AATT_G
Cuts at: 6897 6897
Size: 9298
MluI A'CGCG_T
Cuts at: 9174 9174
Size: 9298
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2688 3603 4032 4369 4608 5575 5717 8412
Size: 1383 915 429 337 239 967 142 2695
Cuts at: 8412 8596 1305
Size: 184 2007
Fragments arranged by size:
2695 2007 1383 967 915 429 337 239
184 142
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2733 4136 4766 5582 10
Size: 54 1425 343 901 1403 630 816 3726
Fragments arranged by size:
3726 1425 1403 901 816 630 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2255 2495 3340 3566 4214 4385 4518
Size: 648 1089 240 845 226 648 171 133
Cuts at: 4518 4938 4953 5054 5429 6190 7256 7615 7774
Size: 420 15 101 375 761 1066 359 159
Cuts at: 7774 518
Size: 2042
Fragments arranged by size:
2042 1089 1066 845 761 648 648 420
375 359 240 226 171 159 133 101
15
MspA1I CmG'CkG
Cuts at: 1606 2147 2484 2532 3169 3411 3474 3799 4183
Size: 541 337 48 637 242 63 325 384
Cuts at: 4183 4671 4842 4965 4996 5199 5223 5454 6714
Size: 488 171 123 31 203 24 231 1260
Cuts at: 6714 7391 8539 8784 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1260 1148 677 637 541 488 384
337 325 245 242 231 203 171 123
63 48 31 24
NaeI GCC'GGC
Cuts at: 3901 5473 3901
Size: 1572 7726
NarI GG'CG_CC
Cuts at: 2049 2331 2049
Size: 282 9016
NcoI C'CATG_G
Cuts at: 513 1065 2022 4513 4689 5364 6157 6277 513
Size: 552 957 2491 176 675 793 120 3534
Fragments arranged by size:
3534 2491 957 793 675 552 176 120
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9298
NgoMIV G'CCGG_C
Cuts at: 3899 5471 3899
Size: 1572 7726
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9298
NotI GC'GGCC_GC
Cuts at: 6745 6745
Size: 9298
NsiI A_TGCA'T
Cuts at: 5939 6418 5939
Size: 479 8819
NspI r_CATG'y
Cuts at: 1132 1229 2635 4273 4390 4417 4815 5316 6193
Size: 97 1406 1638 117 27 398 501 877
Cuts at: 6193 6656 8201 1132
Size: 463 1545 2229
Fragments arranged by size:
2229 1638 1545 1406 877 501 463 398
117 97 27
PasI CC'CwG_GG
Cuts at: 1348 3959 5326 5998 1348
Size: 2611 1367 672 4648
Fragments arranged by size:
4648 2611 1367 672
PciI A'CATG_T
Cuts at: 4413 6189 8197 4413
Size: 1776 2008 5514
Fragments arranged by size:
5514 2008 1776
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2170 3589 4132 5290 1108
Size: 1062 1419 543 1158 5116
Fragments arranged by size:
5116 1419 1158 1062 543
PfoI T'CCnGG_A
Cuts at: 2337 3378 4125 4140 2337
Size: 1041 747 15 7495
Fragments arranged by size:
7495 1041 747 15
PmeI GTTT'AAAC
Cuts at: 6602 6602
Size: 9298
PmlI CAC'GTG
Cuts at: 2877 5659 2877
Size: 2782 6516
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7367 7399 8880 8912 7367
Size: 32 1481 32 7753
Fragments arranged by size:
7753 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2341 3223 4822 6130 6180 2341
Size: 882 1599 1308 50 5459
Fragments arranged by size:
5459 1599 1308 882 50
PshAI GACnn'nnGTC
Cuts at: 1613 3922 4611 1613
Size: 2309 689 6300
Fragments arranged by size:
6300 2309 689
PsiI TTA'TAA
Cuts at: 6868 6868
Size: 9298
PspOMI G'GGCC_C
Cuts at: 3405 5280 5415 6470 3405
Size: 1875 135 1055 6233
Fragments arranged by size:
6233 1875 1055 135
PstI C_TGCA'G
Cuts at: 830 2995 3231 3270 3303 3416 4613 4676 5379
Size: 2165 236 39 33 113 1197 63 703
Cuts at: 5379 5577 5970 6650 830
Size: 198 393 680 3478
Fragments arranged by size:
3478 2165 1197 703 680 393 236 198
113 63 39 33
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9298
PvuII CAG'CTG
Cuts at: 2532 3169 3799 4183 4965 5199 2532
Size: 637 630 384 782 234 6631
Fragments arranged by size:
6631 782 637 630 384 234
RsrII CG'GwC_CG
Cuts at: 5908 5908
Size: 9298
SacII CC_GC'GG
Cuts at: 2485 2485
Size: 9298
SalI G'TCGA_C
Cuts at: 1936 6640 1936
Size: 4704 4594
SanDI GG'GwC_CC
Cuts at: 6130 6130
Size: 9298
SapI GCTCTTCn'nnn_
Cuts at: 3293 3293
Size: 9298
SbfI CC_TGCA'GG
Cuts at: 2995 5577 6650 2995
Size: 2582 1073 5643
Fragments arranged by size:
5643 2582 1073
ScaI AGT'ACT
Cuts at: 7546 9258 7546
Size: 1712 7586
SexAI A'CCwGG_T
Cuts at: 2307 2307
Size: 9298
SfcI C'TryA_G
Cuts at: 826 1045 2991 3164 3227 3266 3299 3412 4609
Size: 219 1946 173 63 39 33 113 1197
Cuts at: 4609 4672 4855 5218 5375 5573 5966 6646 7781
Size: 63 183 363 157 198 393 680 1135
Cuts at: 7781 8462 8653 826
Size: 681 191 1471
Fragments arranged by size:
1946 1471 1197 1135 681 680 393 363
219 198 191 183 173 157 113 63
63 39 33
SfiI GGCCn_nnn'nGGCC
Cuts at: 3837 3837
Size: 9298
SfoI GGC'GCC
Cuts at: 2050 2332 2050
Size: 282 9016
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9298
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9298
SmaI CCC'GGG
Cuts at: 1872 2032 6128 6625 1872
Size: 160 4096 497 4545
Fragments arranged by size:
4545 4096 497 160
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2273 4109 7402 8303 8565 820
Size: 197 926 330 1836 3293 901 262 1553
Fragments arranged by size:
3293 1836 1553 926 901 330 262 197
SnaBI TAC'GTA
Cuts at: 493 6595 7089 493
Size: 6102 494 2702
Fragments arranged by size:
6102 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9298
SphI G_CATG'C
Cuts at: 1132 4273 4390 6656 1132
Size: 3141 117 2266 3774
Fragments arranged by size:
3774 3141 2266 117
SspI AAT'ATT
Cuts at: 5 52 7222 5
Size: 47 7170 2081
Fragments arranged by size:
7170 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9298
StyI C'CwwG_G
Cuts at: 513 1065 2022 2079 3135 4085 4275 4513 4689
Size: 552 957 57 1056 950 190 238 176
Cuts at: 4689 5364 5544 5857 6157 6277 6301 6435 6737
Size: 675 180 313 300 120 24 134 302
Cuts at: 6737 513
Size: 3074
Fragments arranged by size:
3074 1056 957 950 675 552 313 302
300 238 190 180 176 134 120 57
24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3020 4301 4624 4832 5377 7325 7342
Size: 1255 1440 1281 323 208 545 1948 17
Cuts at: 7342 7495 7680 8125 325
Size: 153 185 445 1498
Fragments arranged by size:
1948 1498 1440 1281 1255 545 445 323
208 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2673 3009 5703
Size: 274 80 33 51 1443 696 336 2694
Cuts at: 5703 7544 9256 96
Size: 1841 1712 138
Fragments arranged by size:
2694 1841 1712 1443 696 336 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2792 2824 3518 3550 4060 4092 5218 5250 5352
Size: 32 694 32 510 32 1126 32 102
Cuts at: 5352 5384 6153 6185 9258 9290 2792
Size: 32 769 32 3073 32 2800
Fragments arranged by size:
3073 2800 1126 769 694 510 102 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9298
XbaI T'CTAG_A
Cuts at: 6634 6634
Size: 9298
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3586 4129 5333 3586
Size: 543 1204 7551
Fragments arranged by size:
7551 1204 543
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9298
XmaI C'CCGG_G
Cuts at: 1870 2030 6126 6623 1870
Size: 160 4096 497 4545
Fragments arranged by size:
4545 4096 497 160
XmnI GAAnn'nnTTC
Cuts at: 2477 7427 2477
Size: 4950 4348
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7106 276
Size: 53 83 186 936 5572 2468
Fragments arranged by size:
5572 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI
FseI NruI PacI PspXI PsrI SacI SrfI SwaI