Restriction map for
pFN21ASDA0794
(Circular) (Six-base) MAPSORT of: pFN21ASDA0794.seq Check: 5082 from: 1 to: 6196
pFN21ASDA0794 6196 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4006 278
Size: 53 83 186 936 2470 2468
Fragments arranged by size:
2470 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3539 1937
Size: 1602 4594
Acc65I G'GTAC_C
Cuts at: 3517 3517
Size: 6196
AclI AA'CG_TT
Cuts at: 1563 3373 4323 4696 1563
Size: 1810 950 373 3063
Fragments arranged by size:
3063 1810 950 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2081 2211 4259 5643 5911 1632
Size: 449 130 2048 1384 268 1917
Fragments arranged by size:
2048 1917 1384 449 268 130
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5999
AflIII A'CryG_T
Cuts at: 1203 5095 6072 1203
Size: 3892 977 1327
Fragments arranged by size:
3892 1327 977
AgeI A'CCGG_T
Cuts at: 2093 5027 2093
Size: 2934 3262
AhdI GACnn_n'nnGTC
Cuts at: 4925 4925
Size: 6196
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6196
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2581 1184
Size: 1397 4799
AlwNI CAG_nnn'CTG
Cuts at: 3939 5511 3939
Size: 1572 4624
ApaLI G'TGCA_C
Cuts at: 4253 5409 4253
Size: 1156 5040
ApoI r'AATT_y
Cuts at: 1493 3505 3731 3883 5992 1493
Size: 2012 226 152 2109 1697
Fragments arranged by size:
2109 2012 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4750 160
Size: 872 3718 1606
Fragments arranged by size:
3718 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6196
AvaI C'yCGr_G
Cuts at: 1870 1943 3521 1870
Size: 73 1578 4545
Fragments arranged by size:
4545 1578 73
BamHI G'GATC_C
Cuts at: 3526 3893 3526
Size: 367 5829
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2748 3517 4972 618
Size: 325 217 627 961 769 1455 1842
Fragments arranged by size:
1842 1455 961 769 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2042 2199 3515 1391
Size: 651 157 1316 4072
Fragments arranged by size:
4072 1316 651 157
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2886 928
Size: 971 987 4238
Fragments arranged by size:
4238 987 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4387 4421 1903
Size: 34 2450 34 3678
Fragments arranged by size:
3678 2450 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3288 3353 4088 5298 3288
Size: 65 735 1210 4186
Fragments arranged by size:
4186 1210 735 65
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6196
BfrBI ATG'CAT
Cuts at: 2470 2470
Size: 6196
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4807 136
Size: 107 122 71 631 605 3135 1525
Fragments arranged by size:
3135 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2440 6191 2440
Size: 3751 2445
BlpI GC'TnA_GC
Cuts at: 3613 3613
Size: 6196
Bme1580I G_kGCm'C
Cuts at: 1790 2260 2851 4257 5413 1790
Size: 470 591 1406 1156 2573
Fragments arranged by size:
2573 1406 1156 591 470
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6196
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2193 3927 4885
Size: 416 53 83 399 24 776 1734 958
Cuts at: 4885 442
Size: 1753
Fragments arranged by size:
1753 1734 958 776 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3332 1056
Size: 2276 3920
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3218 4856 1239
Size: 1979 1638 2579
Fragments arranged by size:
2579 1979 1638
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3444 4321 5186 5484 3444
Size: 877 865 298 4156
Fragments arranged by size:
4156 877 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4859 882
Size: 617 3360 2219
Fragments arranged by size:
3360 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3346 3493 3987 493
Size: 711 2142 147 494 2702
Fragments arranged by size:
2702 2142 711 494 147
BsaBI GATnn'nnATC
Cuts at: 6190 6190
Size: 6196
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2048 4003 4385 5020
Size: 53 83 186 936 515 1955 382 635
Cuts at: 5020 275
Size: 1451
Fragments arranged by size:
1955 1451 936 635 515 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2093 4629 5027 5301 5448 5859 2093
Size: 2536 398 274 147 411 2430
Fragments arranged by size:
2536 2430 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2024 2054 1178
Size: 30 165 30 621 30 5320
Fragments arranged by size:
5320 621 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2421 3017 3116 1186
Size: 1235 596 99 4266
Fragments arranged by size:
4266 1235 596 99
BseYI C'CCAG_C
Cuts at: 5399 5895 5399
Size: 496 5700
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2316 3368 1163
Size: 573 580 1052 3991
Fragments arranged by size:
3991 1052 580 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3646 4407 5435 1540
Size: 275 186 1645 761 1028 2301
Fragments arranged by size:
2301 1645 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2314 4257 4342 5413 2314
Size: 1943 85 1071 3097
Fragments arranged by size:
3097 1943 1071 85
BsmI GAATG_Cn'
Cuts at: 3707 3800 3707
Size: 93 6103
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2042 2199 2260 2314 2851 3515 4257
Size: 399 252 157 61 54 537 664 742
Cuts at: 4257 4342 5413 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 664 537 399 252 157
85 61 54
BspEI T'CCGG_A
Cuts at: 5859 5859
Size: 6196
BspHI T'CATG_A
Cuts at: 2644 4085 5815 2644
Size: 1441 1730 3025
Fragments arranged by size:
3025 1730 1441
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2626 3551 844
Size: 1782 925 3489
Fragments arranged by size:
3489 1782 925
BsrBI CCG'CTC
Cuts at: 1394 1823 2961 4083 1394
Size: 429 1138 1122 3507
Fragments arranged by size:
3507 1138 1122 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3434 4691 4865 65
Size: 1183 2186 1257 174 1396
Fragments arranged by size:
2186 1396 1257 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2093 4840 5027 1700
Size: 110 283 2747 187 2869
Fragments arranged by size:
2869 2747 283 187 110
BsrGI T'GTAC_A
Cuts at: 96 3475 96
Size: 3379 2817
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6196
BssSI C'ACGA_G
Cuts at: 4256 5268 4256
Size: 1012 5184
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6196
BstBI TT'CG_AA
Cuts at: 2007 5000 2007
Size: 2993 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6196
BstYI r'GATC_y
Cuts at: 1972 2440 3526 3893 4278 4295 5747 5833 5845
Size: 468 1086 367 385 17 1452 86 12
Cuts at: 5845 6191 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1086 468 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2032 2043 2720 513
Size: 552 957 10 11 677 3989
Fragments arranged by size:
3989 957 677 552 11 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2592 487
Size: 683 56 1366 4091
Fragments arranged by size:
4091 1366 683 56
BtsI GCAGTG_nn'
Cuts at: 3004 3712 4506 4526 3004
Size: 708 794 20 4674
Fragments arranged by size:
4674 794 708 20
ClaI AT'CG_AT
Cuts at: 1554 2415 1554
Size: 861 5335
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2482 2501 2517 2536 529
Size: 35 1918 19 16 19 4189
Fragments arranged by size:
4189 1918 35 19 19 16
DraI TTT'AAA
Cuts at: 3500 3500
Size: 6196
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5203 809
Size: 507 3887 1802
Fragments arranged by size:
3887 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2450 2503 3643
Size: 54 1425 50 275 18 620 53 1140
Cuts at: 3643 4532 6161 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1140 889 620 275 54 53
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3643 1537
Size: 275 1831 4090
Fragments arranged by size:
4090 1831 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2187 3033 3312 4126 1353
Size: 834 846 279 814 3423
Fragments arranged by size:
3423 846 834 814 279
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4783 5157 5303 387
Size: 1064 138 3194 374 146 1280
Fragments arranged by size:
3194 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 2211 3218 4259 4856 5643 5911
Size: 393 449 130 1007 1041 597 787 268
Cuts at: 5911 1239
Size: 1524
Fragments arranged by size:
1524 1041 1007 787 597 449 393 268
130
EcoRI G'AATT_C
Cuts at: 3505 3883 3505
Size: 378 5818
FspI TGC'GCA
Cuts at: 4702 4702
Size: 6196
HaeII r_GCGC'y
Cuts at: 1678 2060 5343 1678
Size: 382 3283 2531
Fragments arranged by size:
3283 2531 382
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2397 2429 2945 2957 2977
Size: 32 135 32 825 32 516 12 20
Cuts at: 2977 2989 2996 3028 4837 4869 4911 4943 1373
Size: 12 7 32 1809 32 42 32 2626
Fragments arranged by size:
2626 1809 825 516 135 42 32 32
32 32 32 32 20 12 12 7
HincII GTy'rAC
Cuts at: 669 1657 1938 3540 3786 669
Size: 988 281 1602 246 3079
Fragments arranged by size:
3079 1602 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6196
HpaI GTT'AAC
Cuts at: 3786 3786
Size: 6196
KpnI G_GTAC'C
Cuts at: 3521 3521
Size: 6196
MfeI C'AATT_G
Cuts at: 3795 3795
Size: 6196
MluI A'CGCG_T
Cuts at: 6072 6072
Size: 6196
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5310 5494 1305
Size: 4005 184 2007
Fragments arranged by size:
4005 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2505 10
Size: 54 1425 343 673 3701
Fragments arranged by size:
3701 1425 673 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2563 2649 4154 4513 4672 518
Size: 648 1397 86 1505 359 159 2042
Fragments arranged by size:
2042 1505 1397 648 359 159 86
MspA1I CmG'CkG
Cuts at: 1606 2045 3118 3179 3265 3612 4289 5437 5682
Size: 439 1073 61 86 347 677 1148 245
Cuts at: 5682 1606
Size: 2120
Fragments arranged by size:
2120 1148 1073 677 439 347 245 86
61
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4687
Fragments arranged by size:
4687 957 552
NdeI CA'TA_TG
Cuts at: 387 2121 387
Size: 1734 4462
NheI G'CTAG_C
Cuts at: 1052 3328 1052
Size: 2276 3920
NotI GC'GGCC_GC
Cuts at: 3643 3643
Size: 6196
NsiI A_TGCA'T
Cuts at: 2472 2472
Size: 6196
NspI r_CATG'y
Cuts at: 1132 1229 2562 3554 5099 1132
Size: 97 1333 992 1545 2229
Fragments arranged by size:
2229 1545 1333 992 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6196
PciI A'CATG_T
Cuts at: 5095 5095
Size: 6196
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2306 1108
Size: 1198 4998
PfoI T'CCnGG_A
Cuts at: 2355 2613 3145 2355
Size: 258 532 5406
Fragments arranged by size:
5406 532 258
PmeI GTTT'AAAC
Cuts at: 3500 3500
Size: 6196
PmlI CAC'GTG
Cuts at: 3346 3346
Size: 6196
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4265 4297 5778 5810 4265
Size: 32 1481 32 4651
Fragments arranged by size:
4651 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6196
PsiI TTA'TAA
Cuts at: 2682 3766 2682
Size: 1084 5112
PstI C_TGCA'G
Cuts at: 830 3548 830
Size: 2718 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6196
PvuII CAG'CTG
Cuts at: 3179 3265 3179
Size: 86 6110
SacII CC_GC'GG
Cuts at: 2046 2046
Size: 6196
SalI G'TCGA_C
Cuts at: 1936 3538 1936
Size: 1602 4594
SapI GCTCTTCn'nnn_
Cuts at: 2187 2187
Size: 6196
SbfI CC_TGCA'GG
Cuts at: 3548 3548
Size: 6196
ScaI AGT'ACT
Cuts at: 4444 6156 4444
Size: 1712 4484
SfcI C'TryA_G
Cuts at: 826 1045 3544 4679 5360 5551 826
Size: 219 2499 1135 681 191 1471
Fragments arranged by size:
2499 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6196
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6196
SmaI CCC'GGG
Cuts at: 1872 3523 1872
Size: 1651 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3459 4300 5201 5463 820
Size: 197 926 1516 841 901 262 1553
Fragments arranged by size:
1553 1516 926 901 841 262 197
SnaBI TAC'GTA
Cuts at: 493 3493 3987 493
Size: 3000 494 2702
Fragments arranged by size:
3000 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6196
SphI G_CATG'C
Cuts at: 1132 3554 1132
Size: 2422 3774
SspI AAT'ATT
Cuts at: 5 52 2608 4120 5
Size: 47 2556 1512 2081
Fragments arranged by size:
2556 2081 1512 47
StuI AGG'CCT
Cuts at: 1517 3450 1517
Size: 1933 4263
StyI C'CwwG_G
Cuts at: 513 1065 2022 3166 3635 513
Size: 552 957 1144 469 3074
Fragments arranged by size:
3074 1144 957 552 469
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2470 2786 4223 4240 4393 4578 5023
Size: 1255 890 316 1437 17 153 185 445
Cuts at: 5023 325
Size: 1498
Fragments arranged by size:
1498 1437 1255 890 445 316 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3475 4442 6154
Size: 274 80 33 51 1443 1498 967 1712
Cuts at: 6154 96
Size: 138
Fragments arranged by size:
1712 1498 1443 967 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2024 2056 3379 3411 6156 6188 2024
Size: 32 1323 32 2745 32 2032
Fragments arranged by size:
2745 2032 1323 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6196
XbaI T'CTAG_A
Cuts at: 3532 3532
Size: 6196
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6196
XmaI C'CCGG_G
Cuts at: 1870 3521 1870
Size: 1651 4545
XmnI GAAnn'nnTTC
Cuts at: 2250 2376 4325 2250
Size: 126 1949 4121
Fragments arranged by size:
4121 1949 126
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4004 276
Size: 53 83 186 936 2470 2468
Fragments arranged by size:
2470 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII
SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PpuMI PspOMI
PspXI PsrI RsrII SacI SanDI SexAI SfiI SfoI
SrfI SwaI XcmI