Restriction map for
pFN21ASDA0792
(Circular) (Six-base) MAPSORT of: pFN21ASDA0792.seq Check: 3251 from: 1 to: 7150
pFN21ASDA0792 7150 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2881 4175 2881
Size: 1294 5856
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2196 4960 278
Size: 53 83 186 936 660 2764 2468
Fragments arranged by size:
2764 2468 936 660 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3471 3540 3981 4493 1937
Size: 1534 69 441 512 4594
Fragments arranged by size:
4594 1534 512 441 69
Acc65I G'GTAC_C
Cuts at: 4471 4471
Size: 7150
AclI AA'CG_TT
Cuts at: 1563 5277 5650 1563
Size: 3714 373 3063
Fragments arranged by size:
3714 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3131 5213 6597 6865 1632
Size: 1499 2082 1384 268 1917
Fragments arranged by size:
2082 1917 1499 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6953
AflIII A'CryG_T
Cuts at: 1203 2772 2783 6049 7026 1203
Size: 1569 11 3266 977 1327
Fragments arranged by size:
3266 1569 1327 977 11
AgeI A'CCGG_T
Cuts at: 5981 5981
Size: 7150
AhdI GACnn_n'nnGTC
Cuts at: 2290 5879 2290
Size: 3589 3561
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7150
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3752 1184
Size: 2568 4582
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2021 2053 3030 3062 2021
Size: 32 977 32 6109
Fragments arranged by size:
6109 977 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2407 3061 3289 4893 6465 2407
Size: 654 228 1604 1572 3092
Fragments arranged by size:
3092 1604 1572 654 228
ApaLI G'TGCA_C
Cuts at: 5207 6363 5207
Size: 1156 5994
ApoI r'AATT_y
Cuts at: 1493 4459 4685 4837 6946 1493
Size: 2966 226 152 2109 1697
Fragments arranged by size:
2966 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5704 160
Size: 872 4672 1606
Fragments arranged by size:
4672 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7150
AvaI C'yCGr_G
Cuts at: 1870 1943 2090 4475 1870
Size: 73 147 2385 4545
Fragments arranged by size:
4545 2385 147 73
BamHI G'GATC_C
Cuts at: 4008 4480 4847 4008
Size: 472 367 6311
Fragments arranged by size:
6311 472 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2658 3680 4355 4471 5926
Size: 325 217 627 871 1022 675 116 1455
Cuts at: 5926 618
Size: 1842
Fragments arranged by size:
1842 1455 1022 871 675 627 325 217
116
BanII G_rGCy'C
Cuts at: 1391 3165 3192 4469 1391
Size: 1774 27 1277 4072
Fragments arranged by size:
4072 1774 1277 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2861 2903 2982 3655 3865 928
Size: 971 962 42 79 673 210 4213
Fragments arranged by size:
4213 971 962 673 210 79 42
BbvCI CC'TCA_GC
Cuts at: 4190 4190
Size: 7150
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5341 5375 1903
Size: 34 3404 34 3678
Fragments arranged by size:
3678 3404 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2787 5042 6252 2787
Size: 2255 1210 3685
Fragments arranged by size:
3685 2255 1210
BclI T'GATC_A
Cuts at: 1548 3777 1548
Size: 2229 4921
BfrBI ATG'CAT
Cuts at: 3857 3857
Size: 7150
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3791 5761 136
Size: 107 122 71 631 605 2119 1970 1525
Fragments arranged by size:
2119 1970 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7145 7145
Size: 7150
BlpI GC'TnA_GC
Cuts at: 4567 4567
Size: 7150
Bme1580I G_kGCm'C
Cuts at: 1790 5211 6367 1790
Size: 3421 1156 2573
Fragments arranged by size:
3421 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3046 1318
Size: 1728 5422
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4881 5839 442
Size: 416 53 83 399 24 3464 958 1753
Fragments arranged by size:
3464 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7150
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2063 2132 3038 3509 4019 5810 1239
Size: 824 69 906 471 510 1791 2579
Fragments arranged by size:
2579 1791 906 824 510 471 69
Bpu10I CC'TnA_GC
Cuts at: 4190 4190
Size: 7150
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3929 5275 6140 6438 3929
Size: 1346 865 298 4641
Fragments arranged by size:
4641 1346 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3590 3758 5813 882
Size: 617 2091 168 2055 2219
Fragments arranged by size:
2219 2091 2055 617 168
BsaAI yAC'GTr
Cuts at: 493 1204 2784 4279 4447 4941 493
Size: 711 1580 1495 168 494 2702
Fragments arranged by size:
2702 1580 1495 711 494 168
BsaBI GATnn'nnATC
Cuts at: 2311 7144 2311
Size: 4833 2317
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2193 2427 2979 4957
Size: 53 83 186 936 660 234 552 1978
Cuts at: 4957 5339 5974 275
Size: 382 635 1451
Fragments arranged by size:
1978 1451 936 660 635 552 382 234
186 83 53
BsaWI w'CCGG_w
Cuts at: 3609 5583 5981 6255 6402 6813 3609
Size: 1974 398 274 147 411 3946
Fragments arranged by size:
3946 1974 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6925
Fragments arranged by size:
6925 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2314 2702 1186
Size: 1128 388 5634
Fragments arranged by size:
5634 1128 388
BseYI C'CCAG_C
Cuts at: 2083 2353 2959 3165 3289 3988 6353 6849 2083
Size: 270 606 206 124 699 2365 496 2384
Fragments arranged by size:
2384 2365 699 606 496 270 206 124
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2591 2822 1163
Size: 573 855 231 5491
Fragments arranged by size:
5491 855 573 231
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4600 5361 6389 1540
Size: 275 186 2599 761 1028 2301
Fragments arranged by size:
2599 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2148 3192 5211 5296 6367 2148
Size: 1044 2019 85 1071 2931
Fragments arranged by size:
2931 2019 1071 1044 85
BsmI GAATG_Cn'
Cuts at: 4661 4754 4661
Size: 93 7057
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2148 3165 3192 4469 5211 5296 6367
Size: 399 358 1017 27 1277 742 85 1071
Cuts at: 6367 1391
Size: 2174
Fragments arranged by size:
2174 1277 1071 1017 742 399 358 85
27
BspEI T'CCGG_A
Cuts at: 6813 6813
Size: 7150
BspHI T'CATG_A
Cuts at: 3525 5039 6769 3525
Size: 1514 1730 3906
Fragments arranged by size:
3906 1730 1514
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2881 2895 4175 4505 844
Size: 2037 14 1280 330 3489
Fragments arranged by size:
3489 2037 1280 330 14
BsrBI CCG'CTC
Cuts at: 1394 1823 5037 1394
Size: 429 3214 3507
Fragments arranged by size:
3507 3214 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2262 3730 5645 5819 65
Size: 1183 1014 1468 1915 174 1396
Fragments arranged by size:
1915 1468 1396 1183 1014 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2097 3960 5794 5981 1700
Size: 110 287 1863 1834 187 2869
Fragments arranged by size:
2869 1863 1834 287 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7150
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7150
BssSI C'ACGA_G
Cuts at: 5210 6222 5210
Size: 1012 6138
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7150
BstBI TT'CG_AA
Cuts at: 2007 5954 2007
Size: 3947 3203
BstEII G'GTnAC_C
Cuts at: 1184 2155 1184
Size: 971 6179
BstXI CCAn_nnnn'nTGG
Cuts at: 3652 3652
Size: 7150
BstYI r'GATC_y
Cuts at: 1972 2403 4008 4480 4847 5232 5249 6701 6787
Size: 431 1605 472 367 385 17 1452 86
Cuts at: 6787 6799 7145 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1605 1452 472 431 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2326 3231 2326
Size: 905 6245
BtgI C'CryG_G
Cuts at: 513 1065 3096 3357 4410 513
Size: 552 2031 261 1053 3253
Fragments arranged by size:
3253 2031 1053 552 261
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3688 3898 487
Size: 683 56 2462 210 3739
Fragments arranged by size:
3739 2462 683 210 56
BtsI GCAGTG_nn'
Cuts at: 2070 3482 4423 4666 5460 5480 2070
Size: 1412 941 243 794 20 3740
Fragments arranged by size:
3740 1412 941 794 243 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7150
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2914 2949 529
Size: 35 2350 35 4730
Fragments arranged by size:
4730 2350 35 35
DraI TTT'AAA
Cuts at: 4454 4454
Size: 7150
DraIII CAC_nnn'GTG
Cuts at: 3955 3955
Size: 7150
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4321 6157 809
Size: 507 3005 1836 1802
Fragments arranged by size:
3005 1836 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2127 2257 2820
Size: 54 1425 50 275 18 297 130 563
Cuts at: 2820 2935 3099 3783 4597 5486 7115 8
Size: 115 164 684 814 889 1629 43
Fragments arranged by size:
1629 1425 889 814 684 563 297 275
164 130 115 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4597 1537
Size: 275 2785 4090
Fragments arranged by size:
4090 2785 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2639 2858 3314 4064 4209 5080 1353
Size: 1286 219 456 750 145 871 3423
Fragments arranged by size:
3423 1286 871 750 456 219 145
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2719 5737 6111 6257 387
Size: 1064 138 1130 3018 374 146 1280
Fragments arranged by size:
3018 1280 1130 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3190 3190
Size: 7150
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2063 2132 3038 3131 3509 4019 5213
Size: 393 431 69 906 93 378 510 1194
Cuts at: 5213 5810 6597 6865 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1194 906 787 597 510 431 393
378 268 93 69
EcoNI CCTnn'n_nnAGG
Cuts at: 2449 2840 3646 4309 2449
Size: 391 806 663 5290
Fragments arranged by size:
5290 806 663 391
EcoO109I rG'GnC_Cy
Cuts at: 3265 4102 3265
Size: 837 6313
EcoRI G'AATT_C
Cuts at: 4459 4837 4459
Size: 378 6772
FspI TGC'GCA
Cuts at: 5656 5656
Size: 7150
HaeII r_GCGC'y
Cuts at: 1678 4410 6297 1678
Size: 2732 1887 2531
Fragments arranged by size:
2732 2531 1887
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2276 2308 2969 3001 5791
Size: 32 135 32 704 32 661 32 2790
Cuts at: 5791 5823 5865 5897 1373
Size: 32 42 32 2626
Fragments arranged by size:
2790 2626 704 661 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2333 2515 4494 4740 669
Size: 988 281 395 182 1979 246 3079
Fragments arranged by size:
3079 1979 988 395 281 246 182
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7150
HpaI GTT'AAC
Cuts at: 4740 4740
Size: 7150
KpnI G_GTAC'C
Cuts at: 4475 4475
Size: 7150
MfeI C'AATT_G
Cuts at: 4749 4749
Size: 7150
MluI A'CGCG_T
Cuts at: 2772 7026 2772
Size: 4254 2896
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3633 6264 6448 1305
Size: 2328 2631 184 2007
Fragments arranged by size:
2631 2328 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2822 2937 3101 3785 10
Size: 54 1425 343 990 115 164 684 3375
Fragments arranged by size:
3375 1425 990 684 343 164 115 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2999 3779 5108 5467 5626 518
Size: 648 1833 780 1329 359 159 2042
Fragments arranged by size:
2042 1833 1329 780 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2083 2199 2806 2908 3273 4022 4374 4566
Size: 477 116 607 102 365 749 352 192
Cuts at: 4566 5243 6391 6636 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 749 677 607 477 365 352
245 192 116 102
NcoI C'CATG_G
Cuts at: 513 1065 3096 3357 513
Size: 552 2031 261 4306
Fragments arranged by size:
4306 2031 552 261
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7150
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7150
NotI GC'GGCC_GC
Cuts at: 4597 4597
Size: 7150
NsiI A_TGCA'T
Cuts at: 3859 3859
Size: 7150
NspI r_CATG'y
Cuts at: 1132 1229 4258 4508 6053 1132
Size: 97 3029 250 1545 2229
Fragments arranged by size:
3029 2229 1545 250 97
PasI CC'CwG_GG
Cuts at: 1348 3061 3580 1348
Size: 1713 519 4918
Fragments arranged by size:
4918 1713 519
PciI A'CATG_T
Cuts at: 6049 6049
Size: 7150
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2407 3061 4036 1108
Size: 1299 654 975 4222
Fragments arranged by size:
4222 1299 975 654
PfoI T'CCnGG_A
Cuts at: 2040 2406 2766 3048 3084 2040
Size: 366 360 282 36 6106
Fragments arranged by size:
6106 366 360 282 36
PmeI GTTT'AAAC
Cuts at: 4454 4454
Size: 7150
PmlI CAC'GTG
Cuts at: 2784 2784
Size: 7150
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5219 5251 6732 6764 5219
Size: 32 1481 32 5605
Fragments arranged by size:
5605 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3536 1613
Size: 1923 5227
PsiI TTA'TAA
Cuts at: 2234 4720 2234
Size: 2486 4664
PstI C_TGCA'G
Cuts at: 830 2892 4502 830
Size: 2062 1610 3478
Fragments arranged by size:
3478 2062 1610
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7150
PvuII CAG'CTG
Cuts at: 2083 2199 2806 2083
Size: 116 607 6427
Fragments arranged by size:
6427 607 116
SacI G_AGCT'C
Cuts at: 3192 3192
Size: 7150
SalI G'TCGA_C
Cuts at: 1936 4492 1936
Size: 2556 4594
SapI GCTCTTCn'nnn_
Cuts at: 2858 4209 2858
Size: 1351 5799
SbfI CC_TGCA'GG
Cuts at: 2892 4502 2892
Size: 1610 5540
ScaI AGT'ACT
Cuts at: 5398 7110 5398
Size: 1712 5438
SexAI A'CCwGG_T
Cuts at: 2694 2694
Size: 7150
SfcI C'TryA_G
Cuts at: 826 1045 2579 2888 3281 3892 4498 5633 6314
Size: 219 1534 309 393 611 606 1135 681
Cuts at: 6314 6505 826
Size: 191 1471
Fragments arranged by size:
1534 1471 1135 681 611 606 393 309
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7150
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7150
SmaI CCC'GGG
Cuts at: 1872 4477 1872
Size: 2605 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3944 5254 6155 6417 820
Size: 197 926 2001 1310 901 262 1553
Fragments arranged by size:
2001 1553 1310 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4447 4941 493
Size: 3954 494 2702
Fragments arranged by size:
3954 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7150
SphI G_CATG'C
Cuts at: 1132 4508 1132
Size: 3376 3774
SspI AAT'ATT
Cuts at: 5 52 5074 5
Size: 47 5022 2081
Fragments arranged by size:
5022 2081 47
StuI AGG'CCT
Cuts at: 1517 3641 3827 4034 4442 1517
Size: 2124 186 207 408 4225
Fragments arranged by size:
4225 2124 408 207 186
StyI C'CwwG_G
Cuts at: 513 1065 2058 3096 3357 4035 4589 513
Size: 552 993 1038 261 678 554 3074
Fragments arranged by size:
3074 1038 993 678 554 552 261
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5177 5194 5347 5532 5977 325
Size: 1255 3597 17 153 185 445 1498
Fragments arranged by size:
3597 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2679 5396 7108
Size: 274 80 33 51 1443 702 2717 1712
Cuts at: 7108 96
Size: 138
Fragments arranged by size:
2717 1712 1443 702 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2426 2458 2654 2686 3853 3885 7110 7142 2426
Size: 32 196 32 1167 32 3225 32 2434
Fragments arranged by size:
3225 2434 1167 196 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3505 1316
Size: 2189 4961
XbaI T'CTAG_A
Cuts at: 3595 4486 3595
Size: 891 6259
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2788 3839 4417 2788
Size: 1051 578 5521
Fragments arranged by size:
5521 1051 578
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7150
XmaI C'CCGG_G
Cuts at: 1870 4475 1870
Size: 2605 4545
XmnI GAAnn'nnTTC
Cuts at: 3119 5279 3119
Size: 2160 4990
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2194 4958 276
Size: 53 83 186 936 660 2764 2468
Fragments arranged by size:
2764 2468 936 660 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BplI BsiWI
BsmBI BstZ17I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SfiI SfoI SrfI SwaI